Genistein (NPI 031L)

For research use only. Not for use in humans.

目录号:S1342 中文名称:金雀异黄酮,染料木素

Genistein (NPI 031L) Chemical Structure

CAS No. 446-72-0

Genistein (NPI 031L)是一种来源于大豆的植物雌激素,是一种高特异性的蛋白酪氨酸激酶(PTK) 的抑制剂,在NIH-3T3细胞中通过EGF或者胰岛素介导而抑制有丝分裂,IC50分别为12μM和19 μM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 736.52 现货
RMB 569.08 现货
RMB 1417.83 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Genistein (NPI 031L)发表文献20篇:

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Genistein (NPI 031L)是一种来源于大豆的植物雌激素,是一种高特异性的蛋白酪氨酸激酶(PTK) 的抑制剂,在NIH-3T3细胞中通过EGF或者胰岛素介导而抑制有丝分裂,IC50分别为12μM和19 μM。
靶点
EGFR [1] topo II [1]
体外研究

Genistein是一个ATP竞争性抑制剂。在离体酶,受体制剂和包括血小板、淋巴球的整个细胞以及各种各样人工培养的细胞中,Genistein抑制酪氨酸磷酸化。Genistein也会抑制人工培养细胞中EGF受激的磷酸化作用,并且也会抑制Topo II (拓扑异构酶 II)。Genistein抑制人工培养的A431表皮样癌细胞中EGF受激的酪氨酸磷酸化。抑制作用与ATP竞争,而不与基底竞争。[1] Genistein阻断NIH-3T3 细胞中EGF,胰岛素和凝血酶介导的促有丝分裂作用。[2] Genistein也充当GPR30受体的激动剂,结合于PPARγ和雌激素受体。Genistein也结合于PPARγ,作为该受体的激动剂,其Ki为 5.7 μM。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 M{nnPHBzd2yrZnXyZZRqd25iYYPzZZk> M3O5NGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR|Mh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1|MDFOwG0> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd6OUezO{c,OTZ5OEm3N|c9N2F-
BT20 MlnkVJJwdGmoZYLheIlwdiCjc4PhfS=> M2fvSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmSyNEBk\WyuIHzpcoUh[nliTWTUJIF{e2G7LDDJR|UxRTR4IN88US=> NV;MbIJZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3PFk4OzdpPkG2O|g6PzN5PD;hQi=>
ANN-1 NV\rSlJSS3m2b4TvfIlkKGG|c3H5 NFf4dVVEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05|ryP Ml63QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
breast carcinoma cells MULDfZRwfG:6aXOgZZN{[Xl? NY\SdZhRS3m2b4TvfIlkKGWoZnXjeEBwdiCPQ1[tO{BpfW2jbjDidoVie3RiY3HyZ4lvd22jIHPlcIx{NCCLQ{WwQVE2NjIQvF2= NYj1THJ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEKwNVYxOyd-OEKwNVYxOzxxYU6=
3T3 MV;DfZRwfG:6aXOgZZN{[Xl? M4jiO2N6fG:2b4jpZ{Bm\m[nY4Sgc44hO1R|IHPlcIx{NCCLQ{WwQVI1|ryP NF6wWXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96MkCxOlA{Lz56MkCxOlA{RC:jPh?=
colon cells M3nBd2N6fG:2b4jpZ{Bie3OjeR?= MlzsR5l1d3SxeHnjJIVn\mWldDDvckBYcUS{IHj1cYFvKGOxbH;uJINmdGy|LDDJR|UxRTJ5LkhOwG0> MkfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
Hepa1clc7 M{\x[2N6fG:2b4jpZ4l1gSCjc4PhfS=> NGfpdmg4OiCqcoO= M{LhR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGhmeGFzY3zjO{Bk\WyuczDh[pRmeiB5MjDodpMtKEmFNUC9NVHPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
MCF7 MV3GeY5kfGmxbjDhd5NigQ>? M2PFblYhcHK| NVrwOFdtUW6qaXLpeIlwdiCxZjDwbI9z[m:uIHXzeIVzNWmwZIXj[YQhd3KwaYTobY5mKGSnY3HyZo95gWyjc3WgbY4hcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC2JIhzeyxiSVO1NF0zPs7:TR?= M3PY[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEe1O|QzLz5zMEC3OVc1OjxxYU6=
3T3/A31 NFTBTWVEgXSxdH;4bYNqfHliYYPzZZk> NILvW3YyKHSxIEGwNEB2\y:vTB?= NWmzcpVyPzJiaILz M4roRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEKDTFKvZ{Bud3W|ZTDjcI9v\WRiM2SzM2E{OSClZXzsd{BifCBzIITvJFExOCC3Zz;tUEBi\nSncjC3NkBpenNiYomgcoloem:|aX6gZZN{[Xl? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB7Nki2N{c,OTByOU[4OlM9N2F-
SH-SY5Y MmnySpVv[3Srb36gZZN{[Xl? MUPB[49vcXO2IHHjeIl3cXS7IHnuJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJIF{e2G7IHnuJHNJNVO\NWmgcoV2em:kbHHzeI9u[SClZXzsd{BmgHC{ZYPzbY5oKEW|dILv[4VvKHKnY3XweI9zKGKndHGsJGVEPTB;MD6wNFE4|ryP NFHDfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUmwOlI5OCd-MUG5NFYzQDB:L3G+
HeLa MmTNSpVv[3Srb36gZZN{[Xl? MWXBZ5RqfmG2aX;uJI9nKGW|dILv[4VvKHKnc4DvcpNmKGWuZX3lcpQhcW5iSHXMZUBk\WyuczDzeIFjdHlidILhcpNn\WO2ZXSge4l1cCCqdX3hckBGe3S{b3flckBz\WOncITvdkBj\XSjLjygSWM2OD1yLkCwOFHPxE1? NFXEVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUmwOlI5OCd-MUG5NFYzQDB:L3G+
HeLa Mnf6SpVv[3Srb36gZZN{[Xl? NGXEdYpC[3SrdnH0bY9vKG:oIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRiaX6gTIVN[SClZXzsd{B{fGGkbImgeJJidnOoZXP0[YQhf2m2aDDoeY1idiCHc4Tyc4dmdiC{ZXPldJRweiCjbIDoZU4tKEWFNUC9NE4xPDkQvF2= NGPJZXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUmwOlI5OCd-MUG5NFYzQDB:L3G+
Ishikawa Ml;xSZN1em:pZX7pZ{Bie3OjeR?= MVe0JIRigXN? NWPHbFFWTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxs[WyrbnWgdIhwe3CqYYThd4Uh[WO2aY\peJkh[W[2ZYKgOEBl[Xm|IHL5JJBiemFvbnn0do9xcGWwb3ygdoVt\WG|ZTDhd5NigSxiSVO1NF0xNjVzzszN NXjuOXdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK1NFI{ODdpPkGyOVAzOzB5PD;hQi=>
Hepa-1c1c7 MUXGeY5kfGmxbjDhd5NigQ>? M2[2Xmlv\HWldHnvckBw\iCQQVTQTFpyfWmwb37lJJJm\HWldHHz[UBqdiCvb4Xz[UBJ\XCjLUHjNYM4KGOnbHzzJIF{e2W|c3XkJIF{KGS{dXegcIV3\WxicnXxeYlz\WRidH:g[I92[mynIHXufplu\SCjY4Tpeol1gSCkeTDNWHQh[XO|YYmsJGNFRTJ{LkpOwG0> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVzMEW5NEc,OTR3MUC1PVA9N2F-
Hepa-1c1c7 M{i0eGZ2dmO2aX;uJIF{e2G7 NFPqSopKdmirYnn0bY9vKG:oIH3veZNmKEincHGtNYMy[zdiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTR3LkNOwG0> NGewUJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWxNFU6OCd-MUS1NVA2QTB:L3G+
T47D NXHWSG94TXO2cn;n[Y5q[yCjc4PhfS=> MYPFd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJHQ1P0RiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIIP0bY12dGG2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCncYXpeoFt\W62IITvJFExKHCPIHXzeJJi\GmxbDDifUBidGGvYYKgZox2\SCjc4PhfUwhSWO2aY\peJk:OC5yMEJOwG0> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N|Y9N2F-
MCF7 MmPOSZN1em:pZX7pZ{Bie3OjeR?= NH;3PIRGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyIIDNJIV{fHKjZHnvcEBjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MD6wNFPPxE1? Mn[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5OEe0N|YoRjF3N{i3OFM3RC:jPh?=
T47D NFrnfIlGe3S{b3flcolkKGG|c3H5 NXriOHFiTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMECgdG0h\XO2cnHkbY9tKGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlAxQc7:TR?= NVHUfFJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O|g4PDN4PD;hQi=>
MCF7 NGLKOIdGe3S{b3flcolkKGG|c3H5 M4TRXmV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTByIIDNJIV{fHKjZHnvcEBjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MD6wNVPPxE1? NHewNYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe4O|Q{Pid-MUW3PFc1OzZ:L3G+
293T MWPGeY5kfGmxbjDhd5NigQ>? M4XXfGRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gNlk{XCClZXzsd{whUUN3ME2wMlAxOTQQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzOUS2N{c,OTZ{MUm0OlM9N2F-
293T NVH5TZBMTnWwY4Tpc44h[XO|YYm= MVnEbZNxdGGlZX3lcpQhd2ZiW{PIYVE4[mW2YT3ld5Rz[WSrb3yg[pJwdSC{ZXPvcYJqdmGwdDDoeY1idiCHUnHsdIhiKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyPUCuNFI6PM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzOUS2N{c,OTZ{MUm0OlM9N2F-
Vero MnvDR5l1d3SxeHnjbZR6KGG|c3H5 M4rxeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGGocnnjZY4h\3KnZX6gcY9vc2W7IG\ldo8h[2WubIOsJGlEPTB;M{KuPe69VQ>? NEjCdFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkS0NVA3Pid-MU[0OFExPjZ:L3G+
U937 MkPWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmjRO|IhcHK| M4\RdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUmzO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliV2PUMVgh[XO|YYmsJGlEPTB;NElOwG0> MmDBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUiwOVQoRjF5MUW4NFU1RC:jPh?=
RAW 264 MVrGeY5kfGmxbjDhd5NigQ>? MlTKNlQhcHK| NFywdG5KdmirYnn0bY9vKG:oIEGgeYcwdWxiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEh[WOldX31cIF1cW:wIHnuJHJCXyB{NkSgZ4VtdHNiYX\0[ZIhOjRiaILzMEBKSzVyPUG4MlHPxE1? NVjKbVFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNlAzPDZpPkG3N|IxOjR4PD;hQi=>
K562 MnfGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWOyZlk3PSCmYYnz M1uyemdzd3e2aDDpcohq[mm2aX;uJI9nKEt3NkKgZ4VtdHNiYomgXHRVKGG|c3H5JIFnfGW{IEWg[IF6eyxiSVO1NF0yPy53Nt88US=> NUe1RWxjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVExQTJpPkG3OFEyODl{PD;hQi=>
HUVE12 NFfWbXlHfW6ldHnvckBie3OjeR?= M3;sU3JmfmW{c3HsJI9nKGi7ZILv[4VvKHCncn;4bYRmNWmwZIXj[YQhVESKIHHjeIl3cXS7IHnuJIh2dWGwIFjVWmUyOiClZXzsdy=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB4OEm4NEc,OThyNki5PFA9N2F-
HL60 NX74OGlzSW62aXzleYtmdWmlIHHzd4F6 NIDuPW4zPCCqcoO= MmjHRY51cWyndXvlcYlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjCyOEBpenNiYomgZ4xwdm:pZX7pZ{Bie3OjeTygUGM2OD14LkROwG0> NGjCbXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N|U5QSd-MUixOlM2QDl:L3G+
L1210 MWjBcpRqdGW3a3XtbYMh[XO|YYm= MX6yOEBpenN? MonHRY51cWyndXvlcYlkKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgUFEzOTBiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JINtd26xZ3XubYMh[XO|YYmsJGxEPTB;Nj61{txO M{\aVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N|U5QTxxYU6=
MOLT3 MnfQRY51cWyndXvlcYlkKGG|c3H5 M4j2WVI1KGi{cx?= NIrnZpVCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBOV0yWMzDj[YxteyCjZoTldkAzPCCqcoOgZpkh[2yxbn;n[Y5q[yCjc4PhfUwhVEN3ME23MlXPxE1? NGrMR2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N|U5QSd-MUixOlM2QDl:L3G+
MOLT3 Mm\aS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1fOPVQ5KGi{cx?= NVLjSpZ6T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVEOgZ4VtdHNiYX\0[ZIhPDhiaILzMEBKTzVyPUGyMlfPxE1? MkGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
Raji NXvheIlCSW62aXzleYtmdWmlIHHzd4F6 NHPNZYIzPCCqcoO= NI\WV4lCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBT[WqrIHPlcIx{KGGodHXyJFI1KGi{czDifUBkdG:wb3flcolkKGG|c3H5MEBNSzVyPUGzMlfPxE1? M{XDelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N|U5QTxxYU6=
HL60 MlnmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV:0PEBpenN? MV\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IES4JIhzeyxiSVe1NF0yQC5{zszN NHP6cYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N|U5QSd-MUixOlM2QDl:L3G+
L1210 NIrXelJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFe2TGczPCCqcoO= NITyOmRIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBNOTJzMDDj[YxteyCjZoTldkAzPCCqcoOsJGlIPTB;MUiuOe69VQ>? NX7jcHVsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
Raji MnftS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHu3R5c1QCCqcoO= MWTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDSZYpqKGOnbHzzJIFnfGW{IES4JIhzeyxiSVe1NF0yQC54zszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF4M{W4PUc,OThzNkO1PFk9N2F-
KG1a MlHES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NECyUHA1QCCqcoO= NX3ObVZTT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hU0dzYTDj[YxteyCjZoTldkA1QCCqcoOsJGlIPTB;MkROwG0> NU\HW3RDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
L1210 MnTaSpVv[3Srb36gZZN{[Xl? MoLqNUB1dyB{MDD1US=> Ml3nNlQhcHK| NEHKPYxKdmS3Y4Tpc44hd2ZicEG1R2RMVjKEIILlMYV5eHKnc4Ppc44hcW5ibX;1d4UhVDF{MUCgZ4VtdHNiYYSgNUB1dyB{MDD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOU M3nzNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N|U5QTxxYU6=
HEK293 NITkcIJHfW6ldHnvckBie3OjeR?= NUD0XlU5SW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiC{ZXPvcYJqdmGwdDDMXHJj\XSjIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVIz|ryP MkXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|NEOxNlYoRjF6M{SzNVI3RC:jPh?=
HEK293 NGKyS2VHfW6ldHnvckBie3OjeR?= MlvoRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCOWGLhcJBp[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2zNe69VQ>? NVjZUWZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOFMyOjZpPkG4N|Q{OTJ4PD;hQi=>
NIH3T3 MW\GeY5kfGmxbjDhd5NigQ>? NIq1cWdC\2:waYP0JIFkfGm4aYT5JIF1KEOIVGKt[IVtfGGINUC4JI12fGGwdDDlfJBz\XO|ZXSgbY4hVkmKM2SzJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKG[xcoPrc4xqdi2|dHnteYxifGWmIHP1dpJmdnRuIFXDOVA:PC52zszN Mn3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3OUW2PVYoRjF6NUm1Olk3RC:jPh?=
NHEM M4XDU2Z2dmO2aX;uJIF{e2G7 NFTGbYw4OiCqcoO= NYP5XXB{UW6qaXLpeIlwdiCxZjDt[YxidmmwIIP5cpRp\XOrczDpckBPUEWPIHPlcIx{KGG|c3Xzd4VlKGG|IGuxOGNefGirb4XyZYNqdCCrbnPvdpBwemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whUUN3ME2yPU4z|ryP M360b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUOyPVM1Lz5zOUGzNlk{PDxxYU6=
RAW264.7 NIDmeG5HfW6ldHnvckBie3OjeR?= MlPnTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmZidHCqYTDy[Yxm[XOnIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KHC{ZYTy[YF1\WRiMTDodkBj\W[xcnWgUHBUKGOqYXzs[Y5o\SCkeTDlcpp6dWViaX3teY5w[XO|YYmsJGlEPTB;Mk[uOe69VQ>? M37UZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7Mke4PFU1Lz5zOUK3PFg2PDxxYU6=
MDA-kb2 NGHlU4RHfW6ldHnvckBie3OjeR?= MlG4RYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkBqdiCqdX3hckBOTEFva3KyJINmdGy|IHHzd4V{e2WmIHHzJJN1cW23bHH0bY9vKG:oIHz1Z4ln\XKjc3WgZYN1cX[rdImgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|E2OD12LkVOwG0> M333elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUmyNlQ2Lz5zOUW5NlI1PTxxYU6=
MDA-kb2 M3P6NGZ2dmO2aX;uJIF{e2G7 NFvHTpBC\2:waYP0JIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJG1FSS2tYkKgZ4VtdHNiYYPz[ZN{\WRiYYOgd5RqdXWuYYTpc44hd2ZibIXjbYZmemG|ZTDhZ5Rqfmm2eTDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDNVUxRTRwNN88US=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV7MkK0OUc,OTl3OUKyOFU9N2F-
MCF7 MXjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2T6N2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kg[ZhxemW|c3nu[{BpfW2jbjDNR2Y4KGOnbHzzMEBIUTVyPUGw{txO NHfzcJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUixPFYyOid-MUm4NVg3OTJ:L3G+
MDA-MB-436 NFiwd3JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mn33RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcjDlfJBz\XO|aX7nJIh2dWGwIF3ERU1OSi12M{[gZ4VtdHNuIFfJOVA:OTgQvF2= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQThzOE[xNkc,OTl6MUi2NVI9N2F-
FRT NGDN[lFHfW6ldHnvckBie3OjeR?= MXPCbY5lcW6pIHHm[olvcXS7IITvJGNHXFJiZHXseIFHPTB6IH31eIFvfCCneIDy[ZN{\WRiaX6gSnJVKGOnbHzzJINw\XiycnXzd4lv\yCqYXzp[IUhe2Wwc3n0bZZmKFmIUD3INVQ5WS:LMUWyUEBufXSjboSgdJJwfGWrbjDifUBqd2SrZHWgbY5ndHW6IHHzd4F6NCCNZE2xOk44|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh6MEOyN{c,OTl6OECzNlM9N2F-
T47D MorISZN1em:pZX7pZ{Bie3OjeR?= MofEPVYhcHK| Mn;DSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBVPDeGIHPlcIx{KGG|c3Xzd4VlKGG|IHTyeYchdGW4ZXygZ4F2e2mwZzDzeIlufWyjdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iZYH1bZZidGWwdDD0c{AyOCCyTTDld5Rz[WSrb3ygZYZ1\XJiOU[gbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlAy|ryP MmDUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N|IoRjF7OUK4PFMzRC:jPh?=
MCF7 M{fWTGV{fHKxZ3XubYMh[XO|YYm= NIH4TXE6PiCqcoO= NU\LeJZpTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[W[2ZYKgPVYhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBC[3Srdnn0fV0xNjBzzszN MmW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N|IoRjF7OUK4PFMzRC:jPh?=
MCF7 NIrBVpFGe3S{b3flcolkKGG|c3H5 NH;wRZZGe3S{b3flcolkKGGldHn2bZR6KGmwIHz1Z4ln\XKjc3WgeJJidnOoZXP0[YQhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdic4TpcZVt[XSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGWzdXn2ZYxmdnRidH:gNVAheE1iZYP0doFlcW:uIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiQXP0bZZqfHl;MD6wNe69VQ>? M3vq[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUK4PFMzLz5zOUmyPFg{OjxxYU6=
MCF7 M16xS2V{fHKxZ3XubYMh[XO|YYm= NFTZb5BGe3S{b3flcolkKGGldHn2bZR6KGmwIHz1Z4ln\XKjc3WgeJJidnOoZXP0[YQhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdic4TpcZVt[XSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGWzdXn2ZYxmdnRidH:gNVAxKHCPIHXzeJJi\GmxbDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFHjeIl3cXS7PUCuNFHPxE1? M4G2dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUK4PFMzLz5zOUmyPFg{OjxxYU6=
T47D NGTWeINGe3S{b3flcolkKGG|c3H5 M4DQVWV{fHKxZ3XubYMh[WO2aY\peJkhcW5ibIXjbYZmemG|ZTD0doFve2[nY4Tl[EBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yC|dHnteYxifGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h\XG3aY\hcIVvfCC2bzCxNEBxVSCnc4TyZYRqd2xiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCDY4Tpeol1gT1yLkCx{txO MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl{OEizNkc,OTl7Mki4N|I9N2F-
T47D NGrsb5lGe3S{b3flcolkKGG|c3H5 MWC5OkBpenN? MmfKSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBVPDeGIHPlcIx{KGG|c3Xzd4VlKGG|IHTyeYchdGW4ZXygZ4F2e2mwZzDzeIlufWyjdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iZYH1bZZidGWwdDD0c{AyODBicF2g[ZN1emGmaX;sJIFnfGW{IEm2JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygRYN1cX[rdIm9NE4xQM7:TR?= Mkn6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N|IoRjF7OUK4PFMzRC:jPh?=
MCF7 MonvSZN1em:pZX7pZ{Bie3OjeR?= NEPSdHY6PiCqcoO= M1XvUWV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTByIIDNJIV{fHKjZHnvcEBi\nSncjC5OkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGFkfGm4aYT5QVAvOTIQvF2= MkH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N|IoRjF7OUK4PFMzRC:jPh?=
T47D NXzKcIh[TXO2cn;n[Y5q[yCjc4PhfS=> M{LTfmV{fHKxZ3XubYMh[WO2aY\peJkhcW5ibIXjbYZmemG|ZTD0doFve2[nY4Tl[EBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yC|dHnteYxifGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h\XG3aY\hcIVvfCC2bzCxNFAheE1iZYP0doFlcW:uIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiQXP0bZZqfHl;MD62{txO MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl{OEizNkc,OTl7Mki4N|I9N2F-
RAW264.7 M4nEcmFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? M3;qV|I1KGi{cx?= NW\4Tm1sSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDHdolme3NicnXh[4VvfCxiSVO1NF0zPs7:TR?= NVPWXmd{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOlMyPDVpPkKwN|Y{OTR3PD;hQi=>
MCF7 M{TnbmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFLK[no1QCCqcoO= MkPFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUJOwG0> NGPzSI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEixN|UzPCd-MkC4NVM2OjR:L3G+
LoVo MlfHR5l1d3SxeHnjbZR6KGG|c3H5 MXy3NkBpenN? MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUWuPFjPxE1? M2PYNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUK5PVc4Lz5{MUGyPVk4PzxxYU6=
LNCAP NHO0ZWFEgXSxdH;4bYNqfHliYYPzZZk> MWK3NkBpenN? MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUOwMlY2|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF{OUm3O{c,OjFzMkm5O|c9N2F-
HCT116 NUTXVlhxS3m2b4TvfIlkcXS7IHHzd4F6 NGrIV4M4OiCqcoO= NEToeopEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{PC57zszN M4XodVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUK5PVc4Lz5{MUGyPVk4PzxxYU6=
AGS MnLFR5l1d3SxeHnjbZR6KGG|c3H5 MUe3NkBpenN? MlnoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRWdUKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NEGuOlfPxE1? MlvaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMkm5O|coRjJzMUK5PVc4RC:jPh?=
A549 NGDwWnVEgXSxdH;4bYNqfHliYYPzZZk> MUC3NkBpenN? M3zzN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME20N{4xQc7:TR?= M3m0dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUK5PVc4Lz5{MUGyPVk4PzxxYU6=
DU145 NYTSOW1JS3m2b4TvfIlkcXS7IHHzd4F6 MnrkO|IhcHK| M4DyVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFcvOjoQvF2= NF3QVI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGyPVk4Pyd-MkGxNlk6Pzd:L3G+
RAW264.7 NIrOSmVCdnSraX7mcIFudWG2b4L5JIF{e2G7 MV2yOEBpenN? MlPNRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGTOSoFteGijIIDyc4R2[3Srb36geJJm[XSnZDCyJIhzeyCkZX\vdoUhVFCVIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliRVzJV2EtKEmFNUC9NVkvOc7:TR?= NYm2XXRJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFg4OjdpPkKxNlg5PzJ5PD;hQi=>
MDCK Ml\CSpVv[3Srb36gZZN{[Xl? MYTJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJIlvKE2GQ1ugZ4VtdHNidYPpcochUG:nY3jzeEA{OzN2MjDzeIFqdmmwZzygTWM2OD14LkpOwG0> NXvLdHFJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOVQ5ODBpPkKxN|U1QDByPD;hQi=>
MCF-7 MX M1yzUGZ2dmO2aX;uJIF{e2G7 NVTTcZB3UW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CCrbjDNR2YuPyCPWDDj[YxteyC3c3nu[{BJd2WlaIP0JFM{OzR{IIP0ZYlvcW6pLDDJR|UxRThwON88US=> M1TsWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{W0PFAxLz5{MUO1OFgxODxxYU6=
NCI-ADR-RES M2TpV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MljHOFghcHK| NUe5T2l6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUmNKNUGGUj3SSXMh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF0yPi57OEK0{txO NHm3NVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I4PSd-MkG1NVMzPzV:L3G+
MCF12A NF3UV3lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1HmT|Q5KGi{cx?= MnW5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIECuNFYhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVA:OjFwOEe3Ou69VQ>? MnzBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
NCI-ADR-RES NYq0NmVKSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NH\UZXU1QCCqcoO= NYHudYpySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUmNKNUGGUj3SSXMh[2WubIOgbY5kfWKjdHXkJJdqfGhiMUCwJI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVI3NjlzNUVOwG0> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
MCF12A MlvKRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NITLO441QCCqcoO= Mlf0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIEGwNEBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF0{OC5zOUm1{txO M3PDXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN|I4PTxxYU6=
NCI-ADR-RES MYjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2\JWVQ5KGi{cx?= MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAxNjByMzDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODFibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIEWlJIZmfGGuIHLveolv\SC|ZYL1cUwhT0l3ME2zNk4{PTl2zszN MlXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
MCF12A NULQ[49pSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFTuS2g1QCCqcoO= NXLvPWV5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUWNHOTKDIHPlcIx{KGmwY4XiZZRm\CC5aYToJFAvODB|IH7NJI9nKGW|dILh[IlwdCCjbnSgNE4xOSCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiNTWg[oV1[WxiYn;2bY5mKHOncoXtMEBIUTVyPUOyMlM2QTUQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
LNCAP NVj5eXBiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFTrXFM1QCCqcoO= Mk\tRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOTlPBVEBk\WyuczDlfJBz\XO|aX7nJIFv\HKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9N|gvQTB2Nd88US=> M2TtfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN|I4PTxxYU6=
PC3 MkTWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MnjMOFghcHK| M2\2e2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgZY5lem:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIGDDN{Bk\WyuczDpcoN2[mG2ZXSge4l1cCByLk[gcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjdibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUSyMlY2PzoQvF2= NYLJUpVnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF7 MVvBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MluxOFghcHK| NVTGTm1MSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE4xPiCwTTDv[kBme3S{YXTpc4wh[W6mIECuNFchdk1ib3[geIV{fG:|dHXyc45mKGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7IHnuJJBz\XOnbnPlJI9nKG[ndHHsJIJwfmmwZTDz[ZJ2dSCjbnSgUpVU\XK3bTygS2k2OD12NT63NFg5|ryP NVywW5JqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
FRT NHO5bZhHfW6ldHnvckBie3OjeR?= MVGyOEBpenN? MkTjRolv\GmwZzDh[oZqdmm2eTD0c{BETlSUIF[1NFgh\GWuZYTpc44hdXW2YX70JIV5eHKnc4Pl[EBqdiCob4Lzb49tcW5vc4TpcZVt[XSnZDDGVnQh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaX;kbY5mKGmwZnz1fEBu\WG|dYLl[EBieyC\RmCgdZVmdmOqaX7nJJJifGViYX\0[ZIhOjRiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiS3S9PU41|ryP NWnTfHBSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1Olg{OjNpPkKxOVY5OzJ|PD;hQi=>
MCF7 NGHYPG1CdnSrZYP0do9o\W6rYzDhd5NigQ>? M1HxSlEhfG9iMkCgeW0> M1v5TlQ5KGi{cx?= MlLWRY51cWW|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iRWKtdI9{cXSrdnWgUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJFE4[mW2YTDld5Rz[WSrb3ytbY5lfWOnZDDz[YNz\XSnZDDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBifCBzIITvJFIxKHWPIHHmeIVzKDR6IHjyd{BjgSCyaH;zdIhiNWyrZ3j0JJJmeG:{dHXyJINp\W2rbIXtbY5me2OnbnPlJIF{e2G7 NHzJNHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwNFg2QSd-MkG4NFA5PTl:L3G+
Jurkat T NYXsOoFISXCxcITvd4l{KGG|c3H5 MXOzOkBpenN? NHTGTohKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFr1dotifCCWIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJG5mdyCjZoTldkA{PiCqcoOgZpkh\myxdzDjfZRwdWW2com= NHTmcJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG5O|M6Oyd-MkKxPVc{QTN:L3G+
FRT MV7GeY5kfGmxbjDhd5NigQ>? NH7xOYNRd3SnboTpZZRqd25iYXP0bZZqfHliYYSgbJVu[W5iQ1\UVkBl\Wy2YTDGOVA5KG23dHHueE1u\WSrYYTl[EBqd2SrbnWg[ox2gCCneIDy[ZN{\WRiaX6gdoF1KE[UVDDj[YxteyClb3X4dJJme3Orbneg[ox2d3Knc3PlcpQheHKxdHXpckB[TlBvSEG0PHEwOTF3MlygZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCHQ{WwQVfPxE1? NXz4bpFtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyPFEyQDVpPkKyNlgyOTh3PD;hQi=>
Human Huh7.5.1 M4TnZmFvfGm4aYLhcEBie3OjeR?= NEHHSWw4OiCqcoO= NETUfGlCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiSl\IMVEhUjN7OVXNJIlv\mWldHXkJIlvKEi3bXHuJGh2cDdwNT6xJINmdGy|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDd{IHjyd{BjgSCHR1\QJIF{e2G7LDDFR|UxRTF2LkVOwG0> NGCyOoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS0OVMzQCd-MkK0OFU{Ojh:L3G+
CHO NYiwSW5lTnWwY4Tpc44h[XO|YYm= M{[xeGtqKH[jbIXld{Bnd3Jic3;kbZVuKG[udX;y[ZNk\WmwIDixNEB2VSlidYD0ZYtmKGmwIF;BWHAySjNvdILhcpNn\WO2ZXSgR2hQKGOnbHzzMEBMcT1{Lkm2{txO NWC3VnJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|E1OTVpPkKzOVcyPDF3PD;hQi=>
CHO NIDVbZhHfW6ldHnvckBie3OjeR?= M2LRbJBKSzVyII\hcJVmeyCob4Kgd49lcXWvIH\seY9z\XOlZXnuJEgyOCC3TTmgeZB1[WunIHnuJG9CXFBzQkOteJJidnOoZXP0[YQhS0iRIHPlcIx{NCCLQ{WwQVMvQDlyNEZOwG0> NH\5U2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NVQyPSd-MkO1O|E1OTV:L3G+
CHO M3e3UmZ2dmO2aX;uJIF{e2G7 M3y3fmtqKH[jbIXld{Bnd3Jic3;kbZVuKG[udX;y[ZNk\WmwIDixNEB2VSlidYD0ZYtmKGmwIF;BWHAySjFvdILhcpNn\WO2ZXSgR2hQKGOnbHzzMEBMcT13zszN MmPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{G0NVUoRjJ|NUexOFE2RC:jPh?=
CHO M1TxNWZ2dmO2aX;uJIF{e2G7 NYryeGlmeEmFNUCgeoFtfWW|IH\vdkB{d2SrdX2g[ox2d3Knc3PlbY4hMDFyIIXNLUB2eHSja3WgbY4hV0GWUEHCNU11emGwc3\lZ5Rm\CCFSF:gZ4VtdHNuIFnDOVA:QS5zMkCxNe69VQ>? NVTVRolrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|E1OTVpPkKzOVcyPDF3PD;hQi=>
HuH7 M1\2d2FvfGmybHHzcY9lcWGuIHHzd4F6 NHy3WIMzPCCqcoO= NWfnT3R[SW62aYDsZZNud2SrYXygZYN1cX[rdImgZYdicW6|dDDsbZZmeiC|cH;yc5pwcXSnIIP0ZYdmKG:oIGDsZZNud2SrdX2gZoVz\2inaTDBUmtCKGW6cILld5NqdmdiR1\QJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBx[XKjc3n0[UBl\X[nbH;wcYVvfCCjZoTldkAzPCCqcoOgZpkheVKWLWDDVkBidmGueYPpd{whUUN3ME2yNO69VQ>? M2DoU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK1PFQ6Lz5{NEGyOVg1QTxxYU6=
SKBR3 NWXMPJFNS2WubDDjfYNt\SCjc4PhfS=> MlHsOVAhfG9iN{WgeW0> M{e3VVM3KGi{cx?= MVHD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDTT2JTOyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDhZ4N2dXWuYYTpc44h[XRiR{CvS|EheGijc3WgZZQhPTBidH:gO|UhfU1iYX\0[ZIhOzZiaILzJIJ6KHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcocu[mG|ZXSg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m| M1LOSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW2NFA1Lz5{NES1OlAxPDxxYU6=
A431 MlfRR4VtdCCleXPs[UBie3OjeR?= NGLPc5Q2OCC2bzC3OUB2VQ>? M1nQelM3KGi{cx?= NXPGcZB7S2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hSTR|MTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBi[2O3bYXsZZRqd25iYYSgS|AwTzFicHjhd4Uh[XRiNUCgeI8hPzVidV2gZYZ1\XJiM{[gbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz MmXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NU[wNFQoRjJ2NEW2NFA1RC:jPh?=
A431 NFj0O3NE\WyuIHP5Z4xmKGG|c3H5 M2P5bFUxKHSxIEe1JJVO Mnj1N|YhcHK| NGrDb3FE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBCPDNzIHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMj;NJJBp[XOnIHH0JFUxKHSxIEe1JJVOKGGodHXyJFM3KGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJq[yCjbnHsfZNqeyC{ZXzheIl3\SC2bzDjc451em:u NVnjeFQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVYxODRpPkK0OFU3ODB2PD;hQi=>
A431 M3naOGFxd3C2b4Ppd{Bie3OjeR?= NXrYblNKPTBidH:gO|UhfU1? MkHSO|IhcHK| MnroTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCDNEOxJINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEW{YlKgbY4h[2:vcHzleIUhdWWmaYXtJIF{e2W|c3XkJIF{KGyjdHWgZZBweHSxdHnjM45m[3KxdHnjJINmdGy|IHH0JFUxKHSxIEe1JJVOKGGodHXyJFczKGi{czDifUBidm6neHnuJHYuTkmWQz:3MWFCTCC|dHHpcolv\y2kYYPl[EBndG:5IHP5eI9u\XS{aXOgZY5idHm|aYO= MmX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NU[wNFQoRjJ2NEW2NFA1RC:jPh?=
MCF7 NVXsdWt7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVWwMlEhfG9iMkCgeW0> NELDeFk1KGSjeYO= MnW3V5RqdXWuYYTpc44hd2ZiZ4Lve5RpKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYSgNE4yKHSxIEKwJJVOKGGodHXyJFQh\GG7czDifUBOXFRiYYPzZZk> M1L0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEe4N|E1Lz5{NUC3PFMyPDxxYU6=
MCF7 NVmwZmdqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkjBNlAhfG9iMUCwJJVO NFH2[XY1KGSjeYO= NHSwbotIem:5dHigbY5pcWKrdHnvckBqdiCqdX3hckBOS0Z5IHPlcIx{KGG2IEKwJJRwKDFyMDD1UUBi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5 NVH4VYtYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwO|g{OTRpPkK1NFc5OzF2PD;hQi=>
RAW264.7 MVLGeY5kfGmxbjDhd5NigQ>? M1HoNlI3KHWP NX7MbZJsUW6qaXLpeIlwdiCxZjDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCrbjDMVHMwUU[QZ3HtcYEue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BvcXS{aYTld{Bt\X[nbDDheEAzPiC3TTDifUBIemmnc4OgcYV1cG:mIDjSeoIhRSB4LkSgL{8uKDBwNTD1UUktKEGldHn2bZR6RTZwMd88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{N{G1N{c,OjVzMkexOVM9N2F-
RAW264.7 MVvGeY5kfGmxbjDhd5NigQ>? MlPyOk42KHWP NHv6XIxKdmirYnn0bY9vKG:oIH7peJJq[yCxeHnk[UBxem:mdXP0bY9vKGmwIFzQV{9KTk6pYX3tZU1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJI5qfHKrdHXzJIxmfmWuIHH0JFYvPSC3TTDifUBIemmnc4OgcYV1cG:mIDjSeoIhRSB4LkSgL{8uKDBwNTD1UUktKEGldHn2bZR6RTZwM988US=> MkfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkexOVMoRjJ3MUK3NVU{RC:jPh?=
RAW264.7 NH3oXYJHfW6ldHnvckBie3OjeR?= NWXKNplVOTNidV2= M3OwU2lvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgcol1emm2ZYOgcIV3\WxiYYSgNVMhfU1iYomgS5Jq\XO|IH3leIhw\CBqUo\iJF0hPi52IDuvMUAxNjVidV2pMEBC[3Srdnn0fV03NjQQvF2= NXvCfGduRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPTNpPkK1NVI4OTV|PD;hQi=>
J774A1 NEW0UXpHfW6ldHnvckBie3OjeR?= Ml;qOk42KHWP M3;rSmlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgTlc4PEFzIHPlcIx{KGG|c3Xzd4VlKGG|IH7peJJqfGW|IHzleoVtKGG2IE[uOUB2VSCkeTDHdolme3NibXX0bI9lKCiUdnKgQUAyPi53IDuvMUAyNjRidV2pMEBC[3Srdnn0fV0yOy52zszN NH\E[FQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyO|E2Oyd-MkWxNlcyPTN:L3G+
J774A1 NHPMeHpHfW6ldHnvckBie3OjeR?= NIT4fmoyOyC3TR?= MVHJcohq[mm2aX;uJI9nKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIlvKEySUz;JSm5o[W2vYT3zeIlufWyjdHXkJI1wfXOnIFq3O|RCOSClZXzsd{Bie3Onc4Pl[EBieyCwaYTybZRmeyCuZY\lcEBifCBzMzD1UUBjgSCJcnnld5MhdWW2aH;kJEhTfmJiPTCxOk42KCtxLTCxMlQhfU1rLDDBZ5Rqfmm2eU2xN{45|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{N{G1N{c,OjVzMkexOVM9N2F-
J774A1 Mn:zSpVv[3Srb36gZZN{[Xl? NGHw[WUzPiC3TR?= MmfVTY5pcWKrdHnvckBw\iCwaYTybYMhd3irZHWgdJJw\HWldHnvckBqdiCOUGOvTWZP\2GvbXGtd5RqdXWuYYTl[EBud3W|ZTDKO|c1STFiY3XscJMh[XO|ZYPz[YQh[XNibnn0dol1\XNibHX2[Ywh[XRiMk[geW0h[nliR4Lp[ZN{KG2ndHjv[EApWn[kIE2gNVYvPSBtLz2gNU41KHWPKTygRYN1cX[rdIm9NVQvPc7:TR?= NX3mUG1wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPTNpPkK1NVI4OTV|PD;hQi=>
HEK293 NYPubGpRTnWwY4Tpc44h[XO|YYm= MlfZTY5pcWKrdHnvckBw\iCHR1\SJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCyaH;zdIhwenmuYYTpc44h[nliY3jlcYltfW2rbnXzZ4Vv[2ViYYPzZZktKEmFNUC9NlUvOTMQvF2= M{TZflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkC1NVkxLz5{NUKwOVE6ODxxYU6=
Sf9 MYHGeY5kfGmxbjDhd5NigQ>? MVzJcohq[mm2aX;uJI9nKEirc{[teIFo\2WmIHj1cYFvKHKnY3;tZolv[W62IFTOUXQyKGW6cILld5Nm\CCrbjDpcpNm[3RiU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCGTlGgcYV1cHmudILhcpNn\XKjc3WgZYN1cX[rdImgeZNqdmdiNTetZolwfGmweXzheIVlKDR3LXLwJJVvdWW2aInsZZRm\CCxcjDo[Y1qdWW2aInsZZRm\CCxbHnnc452[2ynb4Tp[IUhe3Wkc4TyZZRmeyCjbnSgX|NJZS2DZH;N[ZQh[nlibHnxeYllKHOlaX70bYwtKEmFNUC9N|DPxE1? NGDaNmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwOlk1PCd-MkW0NFY6PDR:L3G+
LNCAP NVnV[mgzS3m2b4TvfIlkcXS7IHHzd4F6 NYfHcFZSOyCmYYnz MnywR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZY5lem:pZX6t[IVx\W6mZX70JGxPS0GSIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[2WubDD2bYFjcWyrdImgZYZ1\XJiMzDkZZl{KGK7IGfTWE0yKGOnbHygdJJwdGmoZYLheIlwdiCjc4PhfUwhUUN3ME2zO{41|ryP NF3jd4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO0NVE{PSd-Mk[zOFEyOzV:L3G+
insect cells M3z5emZ2dmO2aX;uJIF{e2G7 NXvmfWk5OTBibXnudy=> NX75dHRsUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDNRW8uSSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhSlSLIHnud4VkfCClZXzsd{BxemWrbnP1ZoF1\WRiZn;yJFExKG2rboOg[o9tdG:5ZXSgZpkh[WSmaYTpc44hd2Zia4nueZJidWmwZTDhd{B{fWK|dILheIUtKEmFNUC9N{46|ryP NHvwVWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{W3OVQ4Pid-Mke1O|U1PzZ:L3G+
insect cells MnzmSpVv[3Srb36gZZN{[Xl? NWKyUGhUOTBibXnudy=> NUPVW5hbUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDNRW8uSiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhSlSLIHnud4VkfCClZXzsd{BxemWrbnP1ZoF1\WRiZn;yJFExKG2rboOg[o9tdG:5ZXSgZpkh[WSmaYTpc44hd2ZiYnXufplt[W2rbnWgZZMhe3Wkc4TyZZRmNCCLQ{WwQVQvOc7:TR?= NWXYbJN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1O|U1PzZpPkK3OVc2PDd4PD;hQi=>
insect cells NHnl[pBHfW6ldHnvckBie3OjeR?= NWK2fG1SPDBibXnudy=> M3jZd2lvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTVHPMWIh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJGJVUSCrboPlZ5Qh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iYYLibZRz[XK7IHzp[4h1KHWwaYTzJJBz\WmwY4XiZZRm\CCob4KgOFAhdWmwczDmc4xtd3enZDDifUBi\GSrdHnvckBw\iCudXPp[oVzcW5iZHXybZZifGm4ZTDzeYJ{fHKjdHWgcYVie3W{ZXSgZYZ1\XJiMjDodpMh[nliTVHPMWdtd3diYYPzZZktKEmFNUC9Ok45|ryP MkflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3N{W0O|YoRjJ5NUe1OFc3RC:jPh?=
insect cells NWfCN4pkTnWwY4Tpc44h[XO|YYm= MnjlOFAhdWmwcx?= M4\TPWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTVHPMWEh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJGJVUSCrboPlZ5Qh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iYYLibZRz[XK7IHzp[4h1KHWwaYTzJJBz\WmwY4XiZZRm\CCob4KgOFAhdWmwczDmc4xtd3enZDDifUBi\GSrdHnvckBw\iCudXPp[oVzcW5iZHXybZZifGm4ZTDzeYJ{fHKjdHWgcYVie3W{ZXSgZYZ1\XJiMjDodpMh[nliTVHPMWdtd3diYYPzZZktKEmFNUC9PU44|ryP MnHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3N{W0O|YoRjJ5NUe1OFc3RC:jPh?=
RAW264.7 NXnvSFIySW62aX;zeIVweG:{b4TpZ{Bie3OjeR?= MXq0JJRwKDViZHH5dy=> M4PxbGFvfGmxc4Tlc5Bwem:2aXOgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCUQV7LUE1qdmS3Y3XkJI9{fGWxY3zhd5Qh\GmoZnXy[Y51cWG2aX;uJIFnfGW{IESgeI8hPSCmYYnzJIJ6KFSUQWCgZZN{[XluIFnDOVA:OTFwNN88US=> NGrP[HM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG2PVU{Pyd-MkixOlk2Ozd:L3G+
SK-MEL-2 NHH5SWxEgXSxdH;4bYNqfHliYYPzZZk> MnTlOFghcHK| NYjufHUzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOu69VQ>? MoTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
MML1 MWjDfZRwfG:6aXPpeJkh[XO|YYm= NXPIZWY4PDhiaILz NFzz[ZZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOVUxzIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PDMQvF2= NU[zUopSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
MDA-MB-231 MmjRR5l1d3SxeHnjbZR6KGG|c3H5 NFHRN5c1QCCqcoO= NGn4bldEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFPPxE1? NUj6ZY85RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
LN229 MnqyR5l1d3SxeHnjbZR6KGG|c3H5 NVrnN3FzPDhiaILz MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUlIzQSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQ1|ryP NVXzVowyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
NCI-H460 NWnuSnZpS3m2b4TvfIlkcXS7IHHzd4F6 MXi0PEBpenN? NEC1XoZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSES2NEBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUS3{txO M2rPc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
T47D NWnPcotbS3m2b4TvfIlkcXS7IHHzd4F6 MWG0PEBpenN? NF\6bWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PDkQvF2= MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
HepG2 MYjGeY5kfGmxbjDhd5NigQ>? NXzyenZPOSCqch?= NVvHR4xpUW6qaXLpeIlwdiCxZjDJUE03NUmwZIXj[YQhW1SDVEOgZYN1cX[jdHnvckApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCneIDy[ZN{cW6pIIDTWGFVOy2udXPp[oVz[XOnIIDy[U1qdmO3YnH0[YQh\m:{IEGgbJIh[mWob4LlJGlNNTZic4TpcZVt[XSrb36g[o9zKDZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1{ND64{txO NIfUUYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG0NFcxPSd-MkmxOFA4ODV:L3G+
UOCB1 MU\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3yydlczKGi{cx?= NGniPWZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGXPR2IyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxVcXSncj3HcI8h[XO|YYmsJGVEPTB;M{WuOlk2QM7:TR?= NIfGfWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSwO|k4PSd-Mkm0NFc6PzV:L3G+
NB-EBc1 NUX5UYlFeUiWUzDhd5NigQ>? MofydWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LNCAP NGfLcZhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NIXocpRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzMEBKSzVyPUGw{txO M3L6SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEW2NVE{Lz5{OUS1OlEyOzxxYU6=
LNCAP NFz0RYVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3;FXlczKGi{cx?= MnywRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOTlPBVEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTJ2zszN M1Twc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEW2NVE{Lz5{OUS1OlEyOzxxYU6=
LNCAP MkjqRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmfzO|IhcHK| MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyQQ1HQJINmdGy|IHHmeIVzKDd{IHjyd{BqdiCycnXz[Y5k\SCxZjDlcppidHW2YX3p[IUh[nliTWTTJIF{e2G7LDDJR|UxRTNzLkhOwG0> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR3NkGxN{c,Ojl2NU[xNVM9N2F-
LNCAP MmnJSpVv[3Srb36gZZN{[Xl? NGnrR44zNjVidV2= MmrwNlQhcHK| Ml7hTY5pcWKrdHnvckBw\iCKRFHDOk9JW1B7MDDpckBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCDUjDwdo91\WmwIHzleoVtKGG2IEKuOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? NIDxe4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OlEyOyd-Mkm0OVYyOTN:L3G+
BV2 NULmVHRnSW62aXnu[oxidW2jdH;yfUBie3OjeR?= Mk\xRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDCWlIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBvcXS{aXOgc5hq\GVicILv[JVkfGmxbjygTWM2OD1{OD60{txO NUT5[W5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PFI6PDBpPkK5OFgzQTRyPD;hQi=>
MDA-MB-231/beta41 MnnkSZN1em:pZX7pZ{Bie3OjeR?= M1H5elE5KGi{cx?= M1j4T2V{fHKxZ3XubYMh[WO2aY\peJkh[XRiRWLi[ZRiKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKE2GQT3NRk0zOzFxYnX0ZVQyKGOnbHzzJIFnfGW{IEG4JIhzeyCkeTDy[Y5qdGyjIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxOjUQvF2= MnjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NEGyNFYoRjJ7NkSxNlA3RC:jPh?=
Ishikawa NHHZVmdGe3S{b3flcolkKGG|c3H5 NW\RVlNmQTZiaILz MX\Fd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYXzwbIEhcW5iaIXtZY4hUXOqaXvhe4Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JJV{cW6pIICtcol1em:yaHXuc4wh[XNic4Xid5Rz[XSnIITy[YF1\WRiZn;yJFk3KGi{czDmc4xtd3enZDDifUB{fWK|dILheIUh[WSmaYTpc44h[nlic4DlZ5Rzd3Cqb4TvcYV1emmlIH3leIhw\CxiRVO1NF0xNjJ2zszN NF:3VVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0NVIxPid-Mkm2OFEzODZ:L3G+
HEK293 M1vONGZ2dmO2aX;uJIF{e2G7 NE\CZXY1QCCqcoO= M2\6Z2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXIu[mW2YTD0doFve2[nY4Tl[EBqdiCKRVuyPVMh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAyQTl3zszN M3TwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
HEK293 MUXGeY5kfGmxbjDhd5NigQ>? NGjGW481QCCqcoO= NU\QfmFPSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWi2jbIDoZUB1emGwc3\lZ5Rm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjVyMUG5{txO NHzjR489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PC9pPlPoSW1DVDxxYU6=
HT-29 M3\zb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXPHdo94fGhiaX7obYJqfGmxbjDv[kBJd22xIIPhdIlmdnNiKHj1cYFvMSCKVD2yPUBk\WyuczDifUBOXFRiYYPzZZktKEeLPUSuNVHPxE1? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?
HL60 MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1\MdWdzd3e2aDDpcohq[mm2aX;uJI9nKEixbX:gd4FxcWWwczCobJVu[W5rIFjMOlAh[2WubIOgZpkhVVSWIHHzd4F6NCCJSU20Mlgz|ryP M1XvWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
SGC7901 Mk[4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3OxWmdzd3e2aDDpcohq[mm2aX;uJI9nKEixbX:gd4FxcWWwczCobJVu[W5rIGPHR|c6ODFiY3XscJMh[nliTWTUJIF{e2G7LDDHTV02Njd6zszN NULLSnVQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDRxJ{7DbGVOSkx:L3G+
HL60 NFm4eVdHfW6ldHnvckBie3OjeR?= NX3VOI06S2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBIVFWWMTDpckBpfW2jbjDIUFYxKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjCyMXsyNDJvM1jd[IVwgHlvRD3ncJVkd3OnIIXweIFs\SCvZXHzeZJm\CCob4KgN|Ahe2WlczDifUBld3WkbHWgdoVkcXC{b3PhcEBxdG:2IHHuZYx6e2m|LDDLbV04|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?
CHO NF2zPHlHfW6ldHnvckBie3OjeR?= NICxdWxEd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJGdNXVRzIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gNk1cOSx{LUPIYYRmd3i7LVSt[4x2[2:|ZTD1dJRic2VibXXhd5Vz\WRiZn;yJFMxKHOnY4OgZpkh\G:3YnzlJJJm[2mycn;jZYwheGyxdDDhcoFtgXOrczygT4k:P87:TR?= M2rOTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
3T3L1 MXfGeY5kfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKEeOVWS0JIlvKG2xdYPlJFNVO0xzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBqdnO3bHnuMZN1cW23bHH0[YQhOi2mZX;4fU1FNVtzNFPd[4x2[2:|ZTD1dJRic2VuIFnDOVA:OjEQvF2= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
DNMT-1 / DNMT-3a / DNMT-3b ; 

PubMed: 25931004     


Western blot analysis of the effect of physiological concentrations of genistein (0.5–10 µmol/L) on protein expression of the three DNMT subtypes, DNMT1, DNMT3a and DNMT3b in PCa cells.

p-AKT / AKT ; 

PubMed: 27574003     


Genistein inhibits Akt phosphorylation. MCF-7-C3 and T47D cells were treated with genistein at indicated concentrations for 16 h. Phospho-Akt and Akt were detected with western blotting. *P<0.05.

CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; 

PubMed: 27574003     


MCF-7, MCF-7-C3 and T47D cells were treated with genistein (GENI) at indicated concentrations for 48 h. Protein levels of CIP2A and PARP in all three cell lines, and caspase-3, cleaved caspase-3 (C-caspase-3) in MCF-7-C3 and T47D cells were detected with western blot analysis.

Foxo3; 

PubMed: 21639915     


Genistein inhibits EGF-induced translocation of FOXO3. Immunofluorescent staining of experimental monolayers revealed that EGF-induced FOXO3 translocation from the nucleus to the cytosol was inhibited by genistein. This experiment was repeated two times in triplicate.

p-ERK; 

PubMed: 18384126     


Genistein dose-dependently inhibits the phosphorylation of ERK1/2. Cells were treated as above and harvested at day 5 after 5 min exposure to adipogenic cocktail, and whole cell protein were subjected to western blotting analysis.

PPARγ / C/EBPα / αP2 ; 

PubMed: 18384126     


Genistein dose-dependently inhibits adipogenic gene expression. Mouse BMSC cultures were exposed to adipogenic cocktail in the presence of increasing concentrations of genistein. Cells were harvested at day 21, and whole cell proteins were subjected to western blotting analysis. 

25931004 27574003 21639915 18384126
Immunofluorescence
ER-β / pS87 ER-β ; 

PubMed: 25931004     


Cells were treated with DMSO (vehicle) or genistein 1 µmol/L and 10 µmol/L for 4hr. Then, cells were fixed, immunostained with pan ER-β and pS87 antibodies simultaneously and visualized by the confocal microscope. The red fluorescent staining in the first column is for pan ER-β, the green fluorescent staining in the second column is for pS87 and the third column is for both images merged together using image J software.

25931004
体内研究 Genistein对成年动物的乳腺癌,前列腺癌和其它内分泌依赖性肿瘤具有预防作用。Genistein在饮食中剂量依赖性减少了低分化前列腺癌的发病率,下调了雄性激素受体,雌激素受体-α,孕激素受体,表皮生长因子受体,胰岛素样生长因子-I和胞外信号调节激酶-1,但不影响雌激素受体-β和转化生长因子-α的mRNA的表达。膳食genistein可以通过调节特定性激素受体和生长因子信号通路预防乳腺癌和前列腺癌。[4] Genistein与前列腺肿瘤辐射结合更加抑制了原发性肿瘤的生长,并且增加了对其自发性转移到主动脉旁淋巴结的控制,提高了小鼠存活率。奇怪的是,Genistein单独治疗增加了其到淋巴结的转移。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol ''2 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 270.24
化学式

C15H10O5

CAS号 446-72-0
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01982578 Unknown status Dietary Supplement: Genistein|Other: Placebo Alzheimer''s Disease Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia September 1 2017 Not Applicable
NCT02499861 Completed Drug: Decitabine and Genistein Cancer St. Justine''s Hospital July 2015 Phase 1|Phase 2
NCT01628471 Completed Drug: decitabine in combination with genistein Non Small Cell Lung Cancer Uman Pharma|DSM Nutritional Products Inc.|MDEIE Ministry Québec Government|INRS-Institut Armand Frappier Université du Québec November 2012 Phase 1|Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

EGFR Signaling Pathway Map

相关EGFR产品

Tags: 购买Genistein (NPI 031L) | Genistein (NPI 031L)供应商 | 采购Genistein (NPI 031L) | Genistein (NPI 031L)价格 | Genistein (NPI 031L)生产 | 订购Genistein (NPI 031L) | Genistein (NPI 031L)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID