Afatinib (BIBW2992)

目录号:S1011

Afatinib (BIBW2992) Chemical Structure

Molecular Weight(MW): 485.94

Afatinib (BIBW2992)不可逆地抑制EGFR/ErbB,包括EGFR(wt)EGFR(L858R),EGFR(L858R/T790M),ErbB2(HER2)和ErbB4(HER4),在无细胞试验中IC50分别为0.5 nM,0.4 nM,10 nM,14 nM和1 nM。

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RMB 1964.25 现货
RMB 977.68 现货
RMB 1724.11 现货
RMB 4652.69 现货
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客户使用Selleck该产品发表文献38篇:

客户使用该产品的5个实验数据:

  • Immunoblot analysis of serum starved H2030 cells pre-treated with 500 nM or 1 µM of gefitinib, crizotinib, CP-724714, or afatinib for 12 hr, followed by stimulation with EGF, HGF, NRG1, or their combination (50 ng/ml) for 10 min.

    Nature, 2016, 534(7609):647-51. Afatinib (BIBW2992) purchased from Selleck.

    Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Afatinib (BIBW2992)不可逆地抑制EGFR/ErbB,包括EGFR(wt)EGFR(L858R),EGFR(L858R/T790M),ErbB2(HER2)和ErbB4(HER4),在无细胞试验中IC50分别为0.5 nM,0.4 nM,10 nM,14 nM和1 nM。
靶点
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
ErbB4 [2]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 1 nM 10 nM 14 nM
体外研究

BIBW2992不可逆地抑制EGFR/HER2,在体外作用于野生型EGFR ,L858R突变型EGFR, L858R/T790M 双突变型EGFR,及HER2时IC50分别为0.5, 0.4, 10, 14 nM。在体外实验中,BIBW2992不管作用于野生型EGFR/HER2还是突变型EGFR/HER2都显示出强大的活力,与gefitinib作用于L858R突变型EGFR的活力差不多,但是要比gefitinib 作用于L858R-T790M双突变型EGFR的活力大100倍。在体外,BIBW2992有效抑制所有实验类型细胞的EGFR/HER磷酸化作用。比如人类扁平上皮癌A431细胞系表达的野生型EGFR, 鼠类NIH-3T3细胞转染的野生型HER2 , 以及乳腺癌BT-474细胞系和胃癌NCI-N87细胞系表达的内源性HER2。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 NIfCUoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{G3S2lEPTB;MD6wNFU4OiEQvF2= NYDqWYtGW0GQR1XS
KASUMI-1 MmHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7JZYpvUUN3ME2wMlAxQTFzIN88US=> NETyVoZUSU6JRWK=
HCC2218 NWTSTYhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUK1SYtkUUN3ME2wMlAxQTh5IN88US=> NHHlOoJUSU6JRWK=
KARPAS-45 NWXIT4lGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\rfVRIUUN3ME2wMlAyPjdzIN88US=> NH;DOIxUSU6JRWK=
HSC-4 Mli2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPTO2U{UUN3ME2wMlAyPzR6IN88US=> M2jQeHNCVkeHUh?=
CHL-1 NHHVS5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TlfGlEPTB;MD6wNVg{QSEQvF2= MmDJV2FPT0WU
KY821 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoP1TWM2OD1yLkCyNFYh|ryP MXvTRW5ITVJ?
NCI-N87 Mmq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTBwMEK3O|Mh|ryP M1\xZnNCVkeHUh?=
DOK MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHP1TGxKSzVyPUCuNFMyQTRizszN M1TTUHNCVkeHUh?=
DSH1 M2SwN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Xjb2lEPTB;MD6wN|MyPCEQvF2= NGrOR5lUSU6JRWK=
MDA-MB-175-VII NEXtZZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fkTmlEPTB;MD6wN|QyPiEQvF2= NE\BNmRUSU6JRWK=
NCI-H2170 NFrVbFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknFTWM2OD1yLkCzO|I5KM7:TR?= NInWd3JUSU6JRWK=
BT-474 Mne5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTBwMES5PFYh|ryP M4TNcXNCVkeHUh?=
EFO-27 M3jPbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfTd2E{UUN3ME2wMlA2OTh{IN88US=> NHroW5pUSU6JRWK=
NCI-H720 NV;E[mhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnuU2NzUUN3ME2wMlA2OzJ3IN88US=> MkXkV2FPT0WU
PC-14 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjlcpBKSzVyPUCuNFU3PjhizszN NUnEfXFwW0GQR1XS
EC-GI-10 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTBwMEW5NVIh|ryP MWfTRW5ITVJ?
KYSE-450 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPLb5dVUUN3ME2wMlA2QTd6IN88US=> NVHZeHM6W0GQR1XS
BPH-1 NGnwXG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3Xac2lEPTB;MD6wOVk5QCEQvF2= M3qzZ3NCVkeHUh?=
NCI-H292 MlzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTBwME[wPUDPxE1? NVW3UW1pW0GQR1XS
H9 M{Gw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzhTnVKSzVyPUCuNFcyOzJizszN NV[5[W1TW0GQR1XS
NCI-H526 NFfMWW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;aflNmUUN3ME2wMlA4QDJ|IN88US=> Mlv2V2FPT0WU
NCI-H1623 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvwXGJKSzVyPUCuNFgxOjVizszN Mom0V2FPT0WU
KYSE-140 NUnmeoxNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DDbGlEPTB;MD6wPFM{KM7:TR?= M3j3W3NCVkeHUh?=
Ca9-22 M13vPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDRdXJ{UUN3ME2wMlA5OzV|IN88US=> M4LFR3NCVkeHUh?=
AU565 NVTkcpRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzMTWM2OD1yLkC4OVA1KM7:TR?= NIfUbZNUSU6JRWK=
ZR-75-30 NVi0S4J6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4i3WGlEPTB;MD6wPVU1PSEQvF2= MWPTRW5ITVJ?
NCI-H1770 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2P1eWlEPTB;MD6xNFI5KM7:TR?= NGe3R4pUSU6JRWK=
LB2241-RCC MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHS4cmNKSzVyPUCuNVA5QDRizszN NV;BV2FDW0GQR1XS
CAL-39 NHXoTFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\XdJZKSzVyPUCuNVE4QTJizszN MkjKV2FPT0WU
UACC-893 M2Syfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTBwMUG4PFUh|ryP NHW3Nm5USU6JRWK=
LU-65 M2mwb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzCOW9KSzVyPUCuNVI3OzNizszN M1vaZXNCVkeHUh?=
HN Ml7WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PXd2lEPTB;MD6xN|A4PyEQvF2= MmT6V2FPT0WU
TI-73 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfrPW9KSzVyPUCuNVQ4OTVizszN NWK3TZdRW0GQR1XS
KU812 NHW3[pJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInId4xKSzVyPUCuNVU1PTZizszN MYfTRW5ITVJ?
SW13 NIrudYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3[3eGlEPTB;MD6xOlIyPiEQvF2= NXvoSnY2W0GQR1XS
BB30-HNC M2XGZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWe4NlVoUUN3ME2wMlE3PTd4IN88US=> NEm1emVUSU6JRWK=
HSC-2 NYH6bZFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUmyZYhsUUN3ME2wMlE3QDN5IN88US=> MljaV2FPT0WU
BV-173 M1P5c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{THdGlEPTB;MD6xO|U6PiEQvF2= MorYV2FPT0WU
CAL-27 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\6T5UxUUN3ME2wMlE4PjN4IN88US=> MXfTRW5ITVJ?
SBC-1 Mkm3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;JTmlEPTB;MD6xPFk2QSEQvF2= Mn;mV2FPT0WU
CAL-33 M1zPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf1b3BKSzVyPUCuNlAyQDRizszN NV\JT29TW0GQR1XS
NCI-H209 NYT6W2c5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;XTWM2OD1yLkKwOFYh|ryP Mn7LV2FPT0WU
COR-L105 MnLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwMkGwPFQh|ryP NFv1fFZUSU6JRWK=
HSC-3 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwMkGxPVgh|ryP MmrIV2FPT0WU
NCI-H69 MnfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPLTWM2OD1yLkKxOFUyKM7:TR?= M2rWcnNCVkeHUh?=
MOLT-13 M1fqOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnL3TWM2OD1yLkKzPVc2KM7:TR?= NF33b5FUSU6JRWK=
ML-2 NFe3OIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33hfGlEPTB;MD6yOFgxOiEQvF2= M4XJdnNCVkeHUh?=
IGROV-1 M{PpV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTBwMkW5OVIh|ryP NFnHVpVUSU6JRWK=
TK10 MlLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTKTWM2OD1yLkK2O|M5KM7:TR?= MXPTRW5ITVJ?
EoL-1-cell M2m1bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMk[4PVIh|ryP NITBVYJUSU6JRWK=
HO-1-N-1 M4TqPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmP3TWM2OD1yLkK3PFU{KM7:TR?= M2e5c3NCVkeHUh?=
BHY NVXNVGlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHTTWM2OD1yLkK4OlU2KM7:TR?= MYHTRW5ITVJ?
LU-139 Mm\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlL2TWM2OD1yLkOwOVEh|ryP MULTRW5ITVJ?
23132-87 NHjIZ4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWn0SmZjUUN3ME2wMlMyQTJ|IN88US=> M4T3V3NCVkeHUh?=
ACHN NWfxTFlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjxWXRKSzVyPUCuN|E6QTRizszN NWLVfYNmW0GQR1XS
DoTc2-4510 NXr1d2h2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXzTWM2OD1yLkOyNlE6KM7:TR?= M1HZZnNCVkeHUh?=
BB65-RCC MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nXNGlEPTB;MD6zOFU6OyEQvF2= NXzZdnk2W0GQR1XS
HLE MlW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTBwM{WwOFYh|ryP MX3TRW5ITVJ?
HCC1419 NYWxS4N4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILKV3BKSzVyPUCuN|Y1ODhizszN M3qySXNCVkeHUh?=
A388 Mlu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXkTItZUUN3ME2wMlM3QDV5IN88US=> NE[zUWtUSU6JRWK=
NUGC-3 NVXvcZA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LMV2lEPTB;MD6zPFM4OyEQvF2= NVH2emh7W0GQR1XS
KYSE-180 NI\GfVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITrRWNKSzVyPUCuN|kxQDlizszN MYjTRW5ITVJ?
TE-6 NEjyS|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkW3TWM2OD1yLkSwNlQ3KM7:TR?= NVu5UIJUW0GQR1XS
A498 M3\mWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwNEC2O|Qh|ryP MVnTRW5ITVJ?
NCI-H1648 NUP5TG5wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{T2cWlEPTB;MD60OFE5OiEQvF2= NXT2ZotFW0GQR1XS
DU-145 M4nuSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETFOYhKSzVyPUCuOFQ5OzNizszN M4TwW3NCVkeHUh?=
CW-2 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PIW2lEPTB;MD60OlkyPCEQvF2= M3znb3NCVkeHUh?=
A431 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYOxe5VjUUN3ME2wMlQ5OjN|IN88US=> MnPqV2FPT0WU
SAS M1Ljb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnCTWM2OD1yLkS4N|k5KM7:TR?= MV7TRW5ITVJ?
NCI-H2126 NEnteolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjMW3hKSzVyPUCuOVE6OjZizszN NVnjOYVjW0GQR1XS
NCI-H358 M1O1Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzQcIJ5UUN3ME2wMlU{OjFizszN NVjmVZkxW0GQR1XS
FADU M1\3S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j4cGlEPTB;MD61OFQxPCEQvF2= NIrzdZpUSU6JRWK=
ACN M1W2eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2K4d2lEPTB;MD61OVA6PCEQvF2= MX7TRW5ITVJ?
HCC70 NVXjS2RwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTBwNUe2OlEh|ryP M3Tue3NCVkeHUh?=
NCI-H1838 MkCwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{npe2lEPTB;MD62NFI6QSEQvF2= NGT0Z4ZUSU6JRWK=
CAL-54 NXzOOHR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUT1O2Y1UUN3ME2wMlYyPjJzIN88US=> NVi1UWhyW0GQR1XS
NCI-H1975 M3PwUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH2wXoJKSzVyPUCuOlE4OjhizszN NVi3enZQW0GQR1XS
COR-L88 NYH6fpJoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvVTWM2OD1yLk[0NVE{KM7:TR?= Ml;5V2FPT0WU
CTV-1 MnPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzXT3JKSzVyPUCuOlQ{QTJizszN MVLTRW5ITVJ?
EW-18 NFyx[ohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1u5TmlEPTB;MD62PFY6QCEQvF2= NFqyfJRUSU6JRWK=
HCC1806 MlvwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTBwNkm2OlYh|ryP NYnnb5JbW0GQR1XS
HuP-T4 M2LsWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7oc2JHUUN3ME2wMlY6PzZizszN NXvMRmxpW0GQR1XS
MDA-MB-361 NITQeVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLWTWM2OD1yLkeyOlYzKM7:TR?= NVTXbZFFW0GQR1XS
NCI-H1573 NFHsV|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrEWI5SUUN3ME2wMlc{PjNizszN Mnz3V2FPT0WU
KU-19-19 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjpbY15UUN3ME2wMlc4PDZ2IN88US=> MlG1V2FPT0WU
COLO-678 NWnQeZRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\MdZV3UUN3ME2wMlgxQDN2IN88US=> M3jiOHNCVkeHUh?=
C-33-A NUK5WnFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTBwOEGyPVch|ryP MV\TRW5ITVJ?
Calu-3 NV[5VJFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWG1TFY4UUN3ME2wMlgyPDN2IN88US=> MmrLV2FPT0WU
AGS NYfzbZNJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPxbZdKSzVyPUCuPFM3PDhizszN MXrTRW5ITVJ?
SCC-25 NHGzPHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTrTWM2OD1yLki0NVcyKM7:TR?= M3qzTXNCVkeHUh?=
JVM-2 M4PPRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nNUGlEPTB;MD64PFI6QSEQvF2= NFPIWGlUSU6JRWK=
HCE-T NWTrUHNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M376O2lEPTB;MD64PFQzQCEQvF2= NUXK[ph[W0GQR1XS
ES4 NEDLN3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTBwOUe4N|Yh|ryP M1fGTXNCVkeHUh?=
EKVX MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlu4TWM2OD1zLkCxNFY1KM7:TR?= MYLTRW5ITVJ?
D-542MG M1\iWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPHPZlKSzVyPUGuNFExPjRizszN M1vVVXNCVkeHUh?=
SW1116 NI\pSnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLRTWM2OD1zLkCxNlg4KM7:TR?= MornV2FPT0WU
5637 M2HQbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTFwMEG1NUDPxE1? NGCxS4dUSU6JRWK=
HT-1376 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\tbnY1UUN3ME2xMlAyPTF|IN88US=> MUDTRW5ITVJ?
NB69 NF3Mc2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrzOWtGUUN3ME2xMlAzQTR3IN88US=> NFG0WlhUSU6JRWK=
YKG-1 Mn;4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPNU|BKSzVyPUGuNFU1OTlizszN M1PxfnNCVkeHUh?=
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COLO-800 NWrnRoZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnznTWM2OD15LkSxOFQ3KM7:TR?= M2\a[3NCVkeHUh?=
DB M2Tuemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTdwNkGwNFUh|ryP NX\TR3dLW0GQR1XS
Ca-Ski M1OxXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITjSFJKSzVyPUeuOlQzPTlizszN MXHTRW5ITVJ?
HEL NGTaUXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zY[GlEPTB;Nz63NVIxOyEQvF2= MnXVV2FPT0WU
NH-12 M{HVNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEKwRYdKSzVyPUeuO|I5ODhizszN M{nzWXNCVkeHUh?=
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SW900 NGj5eWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DCeGlEPTB;Nz64NlEzOSEQvF2= MnHhV2FPT0WU
JVM-3 MnfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\VTWM2OD15Lki5OVk6KM7:TR?= M{f5O3NCVkeHUh?=
OAW-28 NXPYV|VPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTdwOUS1O|gh|ryP NUXmbZVVW0GQR1XS
KURAMOCHI NYGxR4N{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjOXlJCUUN3ME23Mlk1QDFzIN88US=> NX:2cYk3W0GQR1XS
BE-13 NF;HcopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTDTWM2OD15Lkm4NVEzKM7:TR?= NFvt[YdUSU6JRWK=
VMRC-RCZ NHP6ToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlftTWM2OD16LkCxOlk4KM7:TR?= MUTTRW5ITVJ?
NCI-H727 M3LpVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\ITWM2OD16LkGwOFIh|ryP NInTWnVUSU6JRWK=
SW1710 NHTHbmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrrUHRvUUN3ME24MlE5PTF3IN88US=> NXXVVGd{W0GQR1XS
D-502MG NX7V[25lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4r6[mlEPTB;OD6yPVI5OiEQvF2= NIKxe5VUSU6JRWK=
NCI-H1993 NVLCfWVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjBVnRUUUN3ME24MlM{PjNzIN88US=> NYG0ZWk5W0GQR1XS
SCC-4 MofMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXnc3dKSzVyPUiuOFQxPTlizszN MnPIV2FPT0WU
HAL-01 NIHibHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfkT|dKSzVyPUiuOVE6OSEQvF2= MXjTRW5ITVJ?
GT3TKB MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDTWVc5UUN3ME24MlY4QTZ2IN88US=> NFnLPWtUSU6JRWK=
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COLO-829 M{\tPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M130WmlEPTB;OD64NFk4PiEQvF2= NYq4UoI1W0GQR1XS
OCI-AML2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnKdZZsUUN3ME24Mlk2PDhzIN88US=> NYnJfJd2W0GQR1XS
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EW-11 NImx[mdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTCR3BmUUN3ME25MlE4PjV|IN88US=> Mn36V2FPT0WU
HCE-4 M{Di[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvvTWM2OD17LkKxOVU5KM7:TR?= MYnTRW5ITVJ?
BALL-1 M{\oeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPHUnNVUUN3ME25MlIyQTB5IN88US=> NHP5fXVUSU6JRWK=
SF539 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHX[XVKSzVyPUmuNlM2PjJizszN NGG1d4ZUSU6JRWK=
CAMA-1 NUX3c|R{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXOxVpNSUUN3ME25MlI5OTJ6IN88US=> MXLTRW5ITVJ?
C32 M3fw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlX1TWM2OD17LkOzNFY1KM7:TR?= MVXTRW5ITVJ?
NCI-H1703 NHn3WI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknnTWM2OD17LkO0N|c2KM7:TR?= M3;W[3NCVkeHUh?=
NCI-H1650 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;2cXhKSzVyPUmuOlA6PDZizszN NV7y[GhVW0GQR1XS
NCI-H1563 NH23fWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1q3VmlEPTB;OT63OlA{OSEQvF2= NH7aRWpUSU6JRWK=
CAL-85-1 NInVVIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DVcGlEPTB;OT64O|M5OyEQvF2= M3zvRnNCVkeHUh?=
UM-UC-3 NX[zcpdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7vTWM2OD17Lkm1NFMh|ryP Ml3IV2FPT0WU
BxPC-3 M3TIT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHjTWM2OD1zMD6wPVczKM7:TR?= NUW5UHR5W0GQR1XS
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DEL M4r2Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTFyLkO5OFYh|ryP MV7TRW5ITVJ?
HuCCT1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PCZmlEPTB;MUCuOlA{OyEQvF2= MkizV2FPT0WU
OVCAR-4 NV:0cm5QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFv5NXRKSzVyPUGwMlg5OzVizszN NEPCUXpUSU6JRWK=
CAL-72 MoOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULYN4xnUUN3ME2xNE46OzlzIN88US=> MWjTRW5ITVJ?
HCC1954 MmXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;2TWM2OD1zMT6wO|U1KM7:TR?= NHLRNm5USU6JRWK=
EFO-21 NH7GPZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XLWGlEPTB;MUGuNVA1PSEQvF2= MYjTRW5ITVJ?
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SK-MEL-24 NEToN4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHHTWM2OD1zMT6yN|Y3KM7:TR?= MXXTRW5ITVJ?
UACC-62 NIO5V4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nHSGlEPTB;MUGuNlM3QCEQvF2= M3TrVnNCVkeHUh?=
NCI-H650 NFvlW|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHS2UYtKSzVyPUGxMlI1OThizszN MkPVV2FPT0WU
ALL-PO M2HrUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1u3Z2lEPTB;MUGuNlUyOiEQvF2= MYPTRW5ITVJ?
T84 Ml7NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTFzLkO5NFch|ryP M1;FNHNCVkeHUh?=
IST-MEL1 M3PDXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIroPFJKSzVyPUGxMlQ2PTlizszN M3jDS3NCVkeHUh?=
SW626 MnPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHzTWM2OD1zMT61O|U2KM7:TR?= M3m3eXNCVkeHUh?=
RPMI-2650 NWTXOpcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnj5TWM2OD1zMT61PFg2KM7:TR?= M33ENHNCVkeHUh?=
MC-IXC M2W0fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jJTmlEPTB;MUGuOlczQCEQvF2= M37sfnNCVkeHUh?=
HOP-62 NETudlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUG4cXpsUUN3ME2xNU43PzZ2IN88US=> MUPTRW5ITVJ?
NOS-1 NYTjbm5kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXni[|B[UUN3ME2xNU44OTB7IN88US=> M{XVTXNCVkeHUh?=
SW1573 Ml\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2P3UGlEPTB;MUGuO|I6PSEQvF2= NHTlc4dUSU6JRWK=
WM-115 NWHMT3B7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPlTWM2OD1zMT63OVg5KM7:TR?= MoXaV2FPT0WU
HuO9 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTSTWM2OD1zMT64O|c1KM7:TR?= NHvNem9USU6JRWK=
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MDA-MB-453 NWH4NnF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3[zdWlEPTB;MUGuPVUyPSEQvF2= NF75SplUSU6JRWK=
NCI-H596 M{S5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTF{LkCzOlkh|ryP MkHNV2FPT0WU
A172 MoTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\UbVBKSzVyPUGyMlA4QThizszN NVTsU5JXW0GQR1XS
RS4-11 NVXtRWhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrsUmI{UUN3ME2xNk4yPzJ3IN88US=> NISzUlBUSU6JRWK=
HCC1937 NFzQWlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTF{Lke1OlQh|ryP M1HiTXNCVkeHUh?=
SW837 MlrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjCTWM2OD1zMj65NFI5KM7:TR?= M4TjeXNCVkeHUh?=
NMC-G1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnpSJluUUN3ME2xNk46OjN2IN88US=> MlHZV2FPT0WU
PFSK-1 MoTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTF|LkC2JO69VQ>? NGXqVWpUSU6JRWK=
DBTRG-05MG NXPrNmhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTF|LkC2N|Ih|ryP NYqyOJRPW0GQR1XS
SK-UT-1 NIPGboFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXXTWM2OD1zMz6xNFg{KM7:TR?= NHHvXppUSU6JRWK=
OVCAR-5 NFjvSGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXrfmI{UUN3ME2xN{4yPTl|IN88US=> MYrTRW5ITVJ?
8505C MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzzTWM2OD1zMz6yNFY4KM7:TR?= MlHGV2FPT0WU
SK-MEL-1 MmezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fxRmlEPTB;MUOuO|E{PSEQvF2= MojNV2FPT0WU
LK-2 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH6wcIxKSzVyPUGzMlc3OzlizszN M1ixVHNCVkeHUh?=
OE33 Ml21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTF|Lke4OkDPxE1? MnXCV2FPT0WU
Ramos-2G6-4C10 NWqzT|A{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXEcYNHUUN3ME2xOE4xOjB{IN88US=> MX;TRW5ITVJ?
EW-13 NFz2RpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGmyOYVKSzVyPUG0MlA{PTJizszN MULTRW5ITVJ?
NCI-H1793 M4jCXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHh[FdKSzVyPUG0MlIxQDdizszN M3rkVXNCVkeHUh?=
BHT-101 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTF2LkK0NUDPxE1? MlX4V2FPT0WU
RKO NHLCemdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXXUoJVUUN3ME2xOE42ODd3IN88US=> NVG2RpVZW0GQR1XS
NEC8 M4XDRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWP3ZopbUUN3ME2xOE42PDd{IN88US=> NYLMR2dVW0GQR1XS
PANC-10-05 NGDKd2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfnTWM2OD1zND62NlQ4KM7:TR?= NV7Tc|VuW0GQR1XS
NCI-H2030 NH\TVVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFL6V4pKSzVyPUG0MlY1PDhizszN Mn7GV2FPT0WU
C2BBe1 NFXJXVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTF2Lke2OEDPxE1? NIPHPYtUSU6JRWK=
EM-2 MkWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;3RVhPUUN3ME2xOE45PTd4IN88US=> M4n3O3NCVkeHUh?=
HCC1395 NH;MVphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPUW25KSzVyPUG0Mlk{OzhizszN NUnYUG9nW0GQR1XS
A2058 NFjSbWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTF3LkC0NFkh|ryP NUXO[VZyW0GQR1XS
SW1463 M2TX[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTF3LkC0PFUh|ryP MUTTRW5ITVJ?
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CAL-120 NXj5WFhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoD6TWM2OD1zNT6xOFQ4KM7:TR?= NELVc|BUSU6JRWK=
COLO-824 MnPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTF3LkG1O|kh|ryP NV\mXXhKW0GQR1XS
LNCaP-Clone-FGC Mom2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmmxTWM2OD1zNT60NlQ5KM7:TR?= NFvzW4lUSU6JRWK=
CP66-MEL MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7PTWM2OD1zNT60Olg5KM7:TR?= NFrYdYRUSU6JRWK=
OC-314 Ml6wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnKzTWM2OD1zNT60O|E1KM7:TR?= MYjTRW5ITVJ?
OVCAR-8 M4nCNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF22Rm9KSzVyPUG1Mlg5PjFizszN M4H3PXNCVkeHUh?=
MFE-280 M2m2e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULzPGVFUUN3ME2xOk4xPDh6IN88US=> MUnTRW5ITVJ?
MHH-NB-11 M3XMfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;HfW5KSzVyPUG2MlA5OzlizszN Mn;UV2FPT0WU
SW1990 NFjhWG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTF4LkG3OVUh|ryP MoLKV2FPT0WU
U-266 NFPHU2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XaW2lEPTB;MU[uNlI6QSEQvF2= M2jNUXNCVkeHUh?=
MFM-223 M{P4SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTF4LkO5OlUh|ryP M1;W[XNCVkeHUh?=
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MCF7 NELR[oZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX35[JNGUUN3ME2xOk41PzJ|IN88US=> NXzIeplYW0GQR1XS
SK-MEL-3 NHPodIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTF4LkS3PFch|ryP MlTkV2FPT0WU
HH MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTF4LkWxOlIh|ryP MnPGV2FPT0WU
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HuP-T3 M33xZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrmTWM2OD1zNj62OVc3KM7:TR?= MkXPV2FPT0WU
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NCI-H1092 NWLCbWZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLwNplKSzVyPUG2Mlg{OTZizszN M4K3WnNCVkeHUh?=
MZ2-MEL M{PoeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\Se2lEPTB;MU[uPFQxOyEQvF2= NIHRNGRUSU6JRWK=
ES3 NWrEWpllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nGSmlEPTB;MU[uPVE{PCEQvF2= M1;uZ3NCVkeHUh?=
COLO-684 MlTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTF4Lkm0O|ch|ryP NGXuXGpUSU6JRWK=
NCI-H661 Mo\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TtemlEPTB;MU[uPVY6PCEQvF2= NIXjbY5USU6JRWK=
CP50-MEL-B M3O2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXhb5JmUUN3ME2xO{4xOzR|IN88US=> NEi1ZoRUSU6JRWK=
NCI-H2405 NV3IdXZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLNZmRtUUN3ME2xO{4yQTd2IN88US=> NULSe|JEW0GQR1XS
U-2-OS NIWwfpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPUTWM2OD1zNz61N|A4KM7:TR?= M1\jNnNCVkeHUh?=
RERF-LC-MS M4r0Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkW1TWM2OD1zNz61OVA5KM7:TR?= NX7qeIFxW0GQR1XS
NCI-H2342 NXHEXZd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nuXmlEPTB;MUeuOVU4KM7:TR?= MmTWV2FPT0WU
ES8 M2fMS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTF5Lk[wPFch|ryP MnTSV2FPT0WU
MOLT-16 NGCyXpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTF5Lki5N|Qh|ryP NWXVSG1GW0GQR1XS
RH-18 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\OcoZKSzVyPUG4MlAxOzVizszN NGjWRmlUSU6JRWK=
D-283MED MkTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3ro[2lEPTB;MUiuNFA5QCEQvF2= MlTtV2FPT0WU
RCC10RGB MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTF6LkC1OVkh|ryP MlThV2FPT0WU
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NCI-H2228 NVvCNGk3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PyR2lEPTB;M{KuOlYxOiEQvF2= MVfTRW5ITVJ?
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H-EMC-SS MlH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;pTWM2OD1|Mj65Olc5KM7:TR?= NV7SeWl[W0GQR1XS
ATN-1 NGezV|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk[0TWM2OD1|Mz6xOFE3KM7:TR?= Mm[xV2FPT0WU
D-263MG M1K1[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LufmlEPTB;M{OuPFM4QCEQvF2= MYnTRW5ITVJ?
LU-135 M17QSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYC0fFdLUUN3ME2zN{45QDJ7IN88US=> M3nOWHNCVkeHUh?=
C8166 NWfucoFVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXt[nlKSzVyPUOzMlg5PTRizszN MYfTRW5ITVJ?
T-24 M2LoN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33EcWlEPTB;M{SuNFIxPSEQvF2= MXfTRW5ITVJ?
Mewo MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGq1TnRKSzVyPUO0MlE{PDlizszN M4\CSXNCVkeHUh?=
22RV1 NWjQbYZWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTN2LkG3OVQh|ryP NFT3UpFUSU6JRWK=
KM-H2 M{fp[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEL5[HpKSzVyPUO0MlE4PzlizszN M{mye3NCVkeHUh?=
NCI-H2122 NFHicHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrpTWM2OD1|ND6zOFcyKM7:TR?= NUfYd2hmW0GQR1XS
ABC-1 M3nEd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXe0ZYg6UUN3ME2zOE4{Pjl|IN88US=> MXPTRW5ITVJ?
NCI-H1355 Mn23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfRUVhXUUN3ME2zOE4{QDh7IN88US=> M{PmUXNCVkeHUh?=
NOMO-1 NXf3bmo3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTN2LkW3NFMh|ryP Mk\LV2FPT0WU
MZ7-mel MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfidG9KSzVyPUO0MlY1QDdizszN M3zjSnNCVkeHUh?=
NCI-H810 MmC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4r0cGlEPTB;M{SuO|I4QCEQvF2= NXXJNYlGW0GQR1XS
D-392MG MkjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVX1XZMxUUN3ME2zOU4xPTZ3IN88US=> MVHTRW5ITVJ?
LB771-HNC NFXyWHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYP4V2w4UUN3ME2zOU4yPjV6IN88US=> MWLTRW5ITVJ?
COLO-679 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XuR2lEPTB;M{WuN|g5KM7:TR?= M1LQPHNCVkeHUh?=
SK-PN-DW NFzCSFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTN3LkSzOlkh|ryP NVLwcWNuW0GQR1XS
VA-ES-BJ NF;pVnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfyTWM2OD1|NT60OlQ6KM7:TR?= NFHYUJZUSU6JRWK=
KNS-42 NWm0S4VkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13YPGlEPTB;M{WuOVgzPiEQvF2= NGHtfllUSU6JRWK=
RVH-421 NUXkUHk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTCTlFKSzVyPUO1Mlc3PDVizszN NYr4[o1DW0GQR1XS
P30-OHK M{\6UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TVfWlEPTB;M{WuPFc2OiEQvF2= NUnXbFJHW0GQR1XS
G-402 NUjyUI1{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG[1VWdKSzVyPUO1Mlk5PjdizszN NWjNPY9sW0GQR1XS
S-117 NGDFWIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;k[4YxUUN3ME2zOk4{ODB6IN88US=> NF3Hd2tUSU6JRWK=
RXF393 MmPrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\5UmlEPTB;M{[uN|c5PSEQvF2= NG\WPWNUSU6JRWK=
LCLC-103H NH;vSlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTN4Lke4NFMh|ryP MVXTRW5ITVJ?
U-118-MG MoLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\nWGhJUUN3ME2zOk45QDR5IN88US=> MkLNV2FPT0WU
8-MG-BA MnO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfxdXJuUUN3ME2zOk46PDh|IN88US=> Mk[5V2FPT0WU
MFH-ino M4ewPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1qxNGlEPTB;M{euN|M4OSEQvF2= MVfTRW5ITVJ?
NCI-H23 MoO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFj5O|hKSzVyPUO3MlQyQTZizszN NIj4RnNUSU6JRWK=
NCI-H446 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITPZ|VKSzVyPUO3MlQ5PjJizszN MXHTRW5ITVJ?
LU-99A MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PvU2lEPTB;M{euOVc{OSEQvF2= NFPkflRUSU6JRWK=
KP-N-YN NVmyWoNQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTN5Lk[xPVUh|ryP Mon1V2FPT0WU
SCC-9 NITpdodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml:3TWM2OD1|Nz63PFM5KM7:TR?= NHvwbIpUSU6JRWK=
EFM-19 NELHdZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPRTlU1UUN3ME2zO{44QTZ5IN88US=> MnXQV2FPT0WU
A427 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HsOWlEPTB;M{euPVExPyEQvF2= MoLVV2FPT0WU
LB831-BLC NEPmZ4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILhTmxKSzVyPUO4MlYxOzlizszN NUKzdIVpW0GQR1XS
HOS NHfWWldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nY[GlEPTB;M{muOlI4OyEQvF2= NWT1SmhDW0GQR1XS
LAN-6 NHzIbIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXRTWM2OD12MD6wNVEzKM7:TR?= NWTpZ2JVW0GQR1XS
U251 MnvDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnVfm9QUUN3ME20NE41ODV2IN88US=> Ml\wV2FPT0WU
SNU-387 M1jZSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLuTWM2OD12MD60OVU{KM7:TR?= NGqxU25USU6JRWK=
DMS-79 NGLHe3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M131WmlEPTB;NECuOVg5QSEQvF2= MonOV2FPT0WU
SK-LU-1 Mm\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TnbmlEPTB;NECuOlU3QCEQvF2= NX\YcZkyW0GQR1XS
HT NUXLWHJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVT4SJAxUUN3ME20NE43PjN7IN88US=> MnWwV2FPT0WU
KP-N-YS M2P6PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTRyLk[3Olgh|ryP NXPuU|g6W0GQR1XS
DMS-114 NEj1[mhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGj4PJlKSzVyPUSwMlg{PzRizszN NWLvbZVWW0GQR1XS
HUTU-80 Mn23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DvXmlEPTB;NEGuNFU3QSEQvF2= M3TLUXNCVkeHUh?=
ONS-76 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPxZmNKSzVyPUSxMlQ4ODlizszN M1TEV3NCVkeHUh?=
A673 M4fUNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XJVmlEPTB;NEGuOVE2PyEQvF2= MXTTRW5ITVJ?
HPAF-II NXznfFlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\jTWM2OD12MT62O|A6KM7:TR?= MUfTRW5ITVJ?
UACC-257 NUe4fplxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPZTWM2OD12MT63N|Y6KM7:TR?= MXvTRW5ITVJ?
EW-22 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvVeJRKSzVyPUSxMlg3QTdizszN NIrzOFJUSU6JRWK=
NCI-H1755 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTRzLki3NlUh|ryP MkPDV2FPT0WU
KYSE-150 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\oTWM2OD12Mj6xO|gzKM7:TR?= NXPLVmtoW0GQR1XS
CGTH-W-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTR{LkS5OVUh|ryP NEf4OnZUSU6JRWK=
NB13 M176NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml:0TWM2OD12Mj62NlE4KM7:TR?= M{P0[3NCVkeHUh?=
SW872 M3rwSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLCTWM2OD12Mj62N|MyKM7:TR?= NYfNR|RDW0GQR1XS
KP-4 MnnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEL3OHNKSzVyPUSyMlg5OzJizszN NInFN2JUSU6JRWK=
HCC38 M4LMTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7DXJFKSzVyPUSyMlk3OThizszN MYDTRW5ITVJ?
BB49-HNC MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XIWmlEPTB;NEOuNlM2PiEQvF2= MlTsV2FPT0WU
CAL-62 NIi4OWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHxTWM2OD12Mz60NFI5KM7:TR?= MmfVV2FPT0WU
NCI-H2029 M3n5Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfWd2NKSzVyPUSzMlU2QDFizszN M2XTWXNCVkeHUh?=
M14 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTR2LkGzN|Ih|ryP MV7TRW5ITVJ?
COLO-680N NUW2OHYyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGT2TZlKSzVyPUS0MlM{QSEQvF2= NHfJfFNUSU6JRWK=
Hs-578-T NX70XmZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTKTW9KSzVyPUS1MlAzQTRizszN MkjWV2FPT0WU
MSTO-211H MoPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDLdXZKSzVyPUS1Mlk1QDJizszN MUjTRW5ITVJ?
EW-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjQTWM2OD12NT65Olg6KM7:TR?= MX\TRW5ITVJ?
SW948 M1n2dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnvWoFrUUN3ME20Ok41PDZ{IN88US=> NYW5cGZjW0GQR1XS
NCI-H460 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jyXWlEPTB;NE[uOVI{KM7:TR?= MVvTRW5ITVJ?
CAS-1 NULJVG82T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVW5NJB[UUN3ME20Ok44PzBzIN88US=> MkewV2FPT0WU
TGBC11TKB MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HOUmlEPTB;NEeuNVc4PyEQvF2= M172bnNCVkeHUh?=
HD-MY-Z NIDBRlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHO0d4pKSzVyPUS4Mlc5PiEQvF2= NIfhTndUSU6JRWK=
NCI-H226 MkizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XndGlEPTB;NEmuO|c5PSEQvF2= NXPmbmpiW0GQR1XS
PA-1 MnPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTR7LkizN|Uh|ryP MVLTRW5ITVJ?
LB2518-MEL MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfmendKSzVyPUS5Mlg4OjNizszN NXzUZlQ3W0GQR1XS
A101D Ml;XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDxTWM2OD12OT65OFI2KM7:TR?= M3vpU3NCVkeHUh?=
NCI-H2087 M13DOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPHUVBKSzVyPUS5Mlk3QDJizszN MmOwV2FPT0WU

... Click to View More Cell Line Experimental Data

体内研究 活体研究时按实验对象体重,每千克口服20 mg BIBW2992持续25天,肿瘤产生明显的衰退现象,通过肿瘤切片的免疫组织化学染色观察发现,EGFR和AKT的磷酸化作用收到抑制。因此,正如lapatinib和neratinib, BIBW2992是次代酪氨酸激酶抑制剂,不可逆地抑制人类EGFR2(HER2)和EGFR激酶。BIBW2992不仅能和原代酪氨酸激酶如erlotinib和gefitinib一样有效抑制EGFR突变型,而且还能作用于抗erlotinib和gefitinib的EGFR。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

+ 展开

体外激酶活性实验:

人类EGFR和L858R/T790M双重突变型EGFR 的酪氨酸激酶区域与谷胱甘肽巯基转移酶区域相接,提取酪氨酸激酶区域的方法见补充说明。在有和没有BIBW2992时分别测定酶活。使用杆状病毒系统克隆HER2激酶,用类似提取EGFR激酶域的方法提取HER2激酶域。
细胞实验:

[1]

+ 展开
  • Cell lines: NSCLC细胞
  • Concentrations: 0-10 μM
  • Incubation Time: 1小时
  • Method:

    细胞按1×104密度转移到96孔板上,在无血清培养基上过夜培养,用于EGFR磷酸化实验。第二天添加包含BIBW2992的混合物,培养基在37oC温育1小时。在室温下用100 ng/ml EGF刺激10分钟。细胞用冰冻PBS冲洗,然后每孔用120 μl HEPEX buffer萃取,然后在室温下震荡1小时。每孔中所有2×104细胞都用于HER2磷酸化实验。在涂有链霉亲和素的板上加用封闭液按1:100比例稀释的抗EGFR的生物素和c-erb2/HER2肿瘤蛋白Ab-5-生物素。上述步骤得到的萃取物转移到有抗体包被的孔中,室温下温育1小时。彩色显影的测法见补充说明,吸光度为450纳米。


    (Only for Reference)
动物实验:

[1]

+ 展开
  • Animal Models: 雌性无胸腺NMRI-nu/nu鼠
  • Formulation: 0.5%甲基纤维素-0.4% Tween-80
  • Dosages: 20 mg/kg
  • Administration: 口服处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 485.94
化学式

C24H25ClFN5O3

CAS号 439081-18-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03878524 Not yet recruiting Breast Cancer|Prostate Cancer|Pancreatic Cancer|Acute Myelogenous Leukemia OHSU Knight Cancer Institute|Oregon Health and Science University|Prospect Creek Foundation March 14 2019 Phase 1
NCT03878524 Not yet recruiting Breast Cancer|Prostate Cancer|Pancreatic Cancer|Acute Myelogenous Leukemia OHSU Knight Cancer Institute|Oregon Health and Science University|Prospect Creek Foundation March 14 2019 Phase 1
NCT03827070 Recruiting Non Small Cell Lung Cancer Center Trials & Treatment Europe|Center Trials & Treatment March 5 2019 Phase 1
NCT03827070 Recruiting Non Small Cell Lung Cancer Center Trials & Treatment Europe|Center Trials & Treatment March 5 2019 Phase 1
NCT03727724 Active not recruiting Carcinoma Non-Small-Cell Lung The Netherlands Cancer Institute|Boehringer Ingelheim|Merck Serono International SA December 4 2018 Phase 2
NCT03727724 Active not recruiting Carcinoma Non-Small-Cell Lung The Netherlands Cancer Institute|Boehringer Ingelheim|Merck Serono International SA December 4 2018 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • 回答:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

EGFR Signaling Pathway Map

EGFR Inhibitors with Unique Features

相关EGFR产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID