Gefitinib (ZD1839)

目录号:S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。

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客户购买Selleck的此次产品后发表的文献95篇:

客户使用该产品的14个实验数据:

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。
特性 Gefitinib是有效的EGFR酪氨酸激酶抑制剂。
靶点
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外研究

Gefitinib 作用于高和低表达EGFR的细胞系,包括NR6, NR6M 和 NR6W 细胞系,有效抑制EGFR上全部酪氨酸磷酸化位点。磷酸化位点 Tyr1173和Tyr992敏感性较低,需要高浓度Gefitinib才能抑制。Gefitinib作用于NR6W细胞,高效阻断PLC-γ磷酸化, IC50为27nM。NR6wtEGFR 和NR6M细胞系具有低水平PLC-γ磷酸化, IC50 分别为 43 nM和 369 nM。Gefitinib抑制AKT磷酸化,作用于低EGFR和EGFRvIII表达的细胞系, IC50 分别为220 和263nM。Gefitinib按 0.1 到 0.5μM剂量处理,显著促进而不是废除 NR6M细胞形成集落。然而, 2μM Gefitinib 完全抑制 NR6M 集落形成。Gefitinib 作用于高和低EGFR表达的细胞系,EGF 刺激72小时后,快速抑制EGFR 和ERK 磷酸化,这种作用存在剂量依赖性。[1]Gefitinib抑制 EGF驱动的未转化MCF10A 细胞生长,IC50为 20 nM。[2] Gefitinib 作用于MDA-MB-231细胞,抑制EGF信号。Gefitinib严重抑制细胞数的增多,这种作用存在剂量依赖性。 [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NV6wd3ZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwMEW0JO69VQ>? M4TNTVE3PTF4NEez
NIH3T3 NGDLXVRHfW6ldHnvckBCe3OjeR?= NXHoNZc{OC5{IN88US=> NH:xc3ozKGh? MXjEUXNQ NV;EdldUUW6qaXLpeIlwdiCxZjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBmemKEMTDheZRweGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCxOFQh|ryP NFHIXIEyPjR6MEK4OC=>
COS7 MWPGeY5kfGmxbjDBd5NigQ>? Moi2PVYh|ryP MnvMTY5pcWKrdHnvckBw\iCqdX3hckBLSUt{IG[2NVdHKG23dHHueEBmgHC{ZYPz[YQ> MYixO|E4QDd{Mh?=
A549 M1PBUmN6fG:2b4jpZ{BCe3OjeR?= NUXpO4Y5QTZiaB?= NW\OeW44TE2VTx?= MkXqTWM2OD1zMz61PUDPxE1? MlrONVg{OTN6MEe=
K562 NEfkUJREgXSxdH;4bYMhSXO|YYm= MV65OkBp MlLQSG1UVw>? MUPJR|UxRTlwM{[g{txO MmXwNVg{OTN6MEe=
MDA-MB-231 NEL0NI5EgXSxdH;4bYMhSXO|YYm= NXLXSXVmQTZiaB?= M13iO2ROW09? NFHyZZNKSzVyPUO2MlYyKM7:TR?= NX7mcldQOTh|MUO4NFc>
MOLT4 M4OzU2N6fG:2b4jpZ{BCe3OjeR?= M4rjclk3KGh? NV7xZXFuTE2VTx?= NVLBRVhqUUN3ME2xOU4xOiEQvF2= NXezXXUzOTh|MUO4NFc>
MCF7 MVnDfZRwfG:6aXOgRZN{[Xl? M17ifVUxKM7:TR?= MmniO|IhcA>? MlLnTWM2OD1zMj6wOUDPxE1? Mn;3NVg4PzF6MUm=
BT474 MnLHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYi3NkBp NFfVcopCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP NF\IVWwyQTB{OESyOC=>
HN5 NX\rWoptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIS1UIE4OiCq MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiQNTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghTUN3MDDv[kAxNjBzIN88US=> Mn;PNVkxOjh2MkS=
BA/F3 M{jJdmN6fG:2b4jpZ{BCe3OjeR?= M1\tPVczKGh? NVK1W45pS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? MXyxPVI{QTJ{OR?=
NIH3T3 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkK5O|IhcA>? MlzBS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> NEi3[lkyQTZ4NUO3Oy=>
MCF7 MX MVHGeY5kfGmxbjDBd5NigQ>? MlzjN|AhdWmw MVnJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJJdqfGhiSVO1NEBw\iBzLkGg{txO M1:0T|E6QTN{OU[w
A431 MkHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvHZVE{OTBizszN NF;ZSHA4OiCq MoPnS2k2OD1yLkWxOEDPxE1? NYTve3ZnOTl7Nkm0OlU>
A431 NWnrR5EyU2mwYYPlJGF{e2G7 NH7Qe3MxNjFizszN NVLqb5ZwOSCq NGHNeXVKdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v M3y5flE6QTZ7NE[1
A431 NV;GO|V{U2mwYYPlJGF{e2G7 MmPwNVAxKM7:TR?= MXKxNEBucW5? NXf4eI5rWHKxbH;u[4VlKGmwaHnibZRwenliYXP0bZZqfHlib3[gSWdHNWmwZIXj[YQhTUeIUjDwbI9{eGixconsZZRqd25? MY[xPVk3QTR4NR?=
Sf9 NGO1V2RMcW6jc3WgRZN{[Xl? NVrySYoyOTBibXnu MXzEUXNQ NWrPPGdyUW6qaXLpeIlwdiCxZjDFS2ZTKGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHKnY3XweI9zKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODN|IN88US=> NYLvOXRtOjByNU[0NlU>
NCI-H1975 NVf3PWlkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4m2V|Uh|ryP Mk[xO|IhcA>? MoTLTWM2OD16LkK2JO69VQ>? MlzNNlAyPTF4N{C=
NCI-H1975 MoGxT4lv[XOnIFHzd4F6 M1uxTVExKM7:TR?= NEC4fZpKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NYfucmhjOjBzNUG2O|A>
NCI-H1975 M4LLS2Z2dmO2aX;uJGF{e2G7 MlnNNVAh|ryP MoruTY5pcWKrdHnvckBw\iCHUlLCNkBifXSxcHjvd5Bpd3K7bHH0bY9v NHfpVpozODF3MU[3NC=>
NCI-H1975 MXzGeY5kfGmxbjDBd5NigQ>? NHLiV442KM7:TR?= Mn;oOFghcA>? MX3JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ Mlj2NlAyPTF4N{C=
A375 NYXHNWdpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPFbYg1QCCq NGezTnpKSzVyPUKwMlU2KM7:TR?= NHfqW4UzODNyNEWzOy=>
SMMC7721 M{nT[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkezOFghcA>? MYPJR|UxRTN|LkGyJO69VQ>? NFWxSIEzODNyNEWzOy=>
DU145 NH3KVXNEgXSxdH;4bYMhSXO|YYm= Mnq2OUBl MWPEUXNQ MmHtTWM2OD12Mj6xO{DPxE1? MWWyNFQ3PjV3NR?=
MIAPaCa2 MUXDfZRwfG:6aXOgRZN{[Xl? NVW4THBSPSCm MXfEUXNQ MVXJR|UxRTVyLk[yJO69VQ>? NUXp[ZRGOjB2Nk[1OVU>
PANC1 NH\Wb|hEgXSxdH;4bYMhSXO|YYm= MojROUBl NI\qcpJFVVOR MkLwTWM2OD12MD60NkDPxE1? MkHUNlA1PjZ3NUW=
PC9 NWrvUFdJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NYXFS2tYOjF{MEi4NFI>
PC9 M4[4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDxd|VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIdzd3e2aD3y[ZNqe3SjboSgbJVu[W5iUFO5JINmdGy|IHX4dJJme3OrbnegSWdHWiCHN{S2Y2E4PTBxVEe5NG0hdXW2YX70JJdqfGhiRVO1NEBw\iB5IN88US=> NFnDbFkzOTJyOEiwNi=>
PC3 MoHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXWNVAh|ryP MUe3NkBp MWPEUXNQ MY\JR|UxRTdwNDFOwG0> NFzXZ5AzOTN3M{W0Oi=>
MDA-MB-468 NXviWYdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkD1NVAxKM7:TR?= MkexOFghcA>? MlewSG1UVw>? NFnsXGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> NE\BPZczOTR{OU[zNi=>
Plasmodium falciparum M{O1VZFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w Mn65NlE5OTdyNEW=
NCI-H1993 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLXO|IhcA>? NF\QfGxFVVOR NHvhS5NKSzVyPUCuNUDPxE1? MUiyNVkyPjR|Mx?=
NCI-H292 NHjRbpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;NdFczKGh? M{TuUGROW09? NHXE[|ZKSzVyPUCuNFIh|ryP M3OzXlIyQTF4NEOz
A431 M1vJN2tqdmG|ZTDBd5NigQ>? NYTadY16OiCq NF6yeopFVVOR MYjJcohq[mm2aX;uJI9nKEWJRmKgdIhwe3Cqb4L5cIF1cW:wIHnuJGVITi2|dHnteYxifGWmIHj1cYFvKEF2M{Gge4l1cCCLQ{WwJI9nKDBwMECxJO69VQ>? MXOyNlExOTF|Mh?=
MCF7 NYexS201T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWOxNFDDqM7:Zz;tUC=> MVq0PEBp M3XqSWROW09? NWDrZm83SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnIh\2WwZTD3bZRpKEmFNUCgc4YhOTJwMEWg{txO MlfnNlIyOTlzM{C=
BT474 M4HubGN6fG:2b4jpZ{BCe3OjeR?= NH\QUoYyOCEQvF2= MnHEOFghcA>? MYXDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| M3WxSVIzOTN6M{C4
MCF7 MXTDfZRwfG:6aXOgRZN{[Xl? NEL3[nkyOCEQvF2= MVK0PEBp NV;U[nVOS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDNR2Y4KGOnbHzzJI93\XJvZYjwdoV{e2mwZzDFdoJDOiClZXzsdy=> NETReoQzOjF|OEOwPC=>
HCC827 NV\wdlBIU2mwYYPlJGF{e2G7 NHXCeWgxNjF{NTFOwG0> NHzOT5gzPCCq M2PBTGROW09? M3HuZ2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v M4mwUFIzOzN7M{Sy
HCC827 M4TLNGtqdmG|ZTDBd5NigQ>? NF\DbXoxNjF{NTFOwG0> M2j1e|I1KGh? MlzKSG1UVw>? NHfwVIFKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= NEL2d4wzOjN|OUO0Ni=>
HCC827 M4Dsb2tqdmG|ZTDBd5NigQ>? MnLONE4yOjVizszN NEjtUmozPCCq MWjEUXNQ NFXEZnpKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiQXv0JJBpd3OyaH;yfYxifGmxbh?= MlHRNlI{Ozl|NEK=
A431 NX;SfZF2U2mwYYPlJGF{e2G7 NUTq[2NkOTByIN88US=> MnXBN|AhdWmw NIfiWmpKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN NVLw[mhLOjJ4OUi3PFI>
H460 NXvaUnlES3m2b4TvfIlkKEG|c3H5 NHLNZmg4OiCq NEDaZplKSzVyPUWuOVkh|ryP MW[yNlczPzR2OR?=
HT-29 NEniZlZEgXSxdH;4bYMhSXO|YYm= NELWVJM4OiCq M{DVb2lEPTB;Mz6zOkDPxE1? NHvHSIszOjd{N{S0PS=>
SGC7901 MVnDfZRwfG:6aXOgRZN{[Xl? NG\H[Hk4OiCq MkPUTWM2OD1zMD6yOkDPxE1? MmHzNlI4Ojd2NEm=
A498 M1fDfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNEm4JINmdGy|IHX4dJJme3OrbnegSWdHWiCjbnSgV3JEKHerdHigTWM2OCCxZjC4MlYh|ryP NVzyWIY1OjJ6MUi4OFg>
Calu3 MXfDfZRwfG:6aXOgRZN{[Xl? MWexNEDPxGdxbVy= MUG3NkBp NXzoSXl1UUN3ME2xMlYh|ryP M4PXSFI{OTF4MU[4
FADU MYTDfZRwfG:6aXOgRZN{[Xl? M124WFExKM7:Zz;tUC=> MX63NkBp NHWxSHdKSzVyPUCuNFUh|ryP NXOzRoF4OjNzMU[xOlg>
HCC827 NH\lTmREgXSxdH;4bYMhSXO|YYm= MVGxNEDPxGdxbVy= Ml;0O|IhcA>? MV\JR|UxRTBwMECxOUDPxE1? MlPtNlMyOTZzNki=
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HT-1376 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\BR2lEPTB;Mz6yNFUxPiEQvF2= MYnTRW5ITVJ?
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PANC-10-05 NYjNTlRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\pcnUxUUN3ME2zMlczODd{IN88US=> MV3TRW5ITVJ?
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NCI-H1155 NEfz[WxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTaTWM2OD1|Lki0NVU6KM7:TR?= M3zEVnNCVkeHUh?=
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NCI-H69 MnzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nsVmlEPTB;Mz65NFE4QCEQvF2= NX3DRXByW0GQR1XS
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NH-12 M2W1RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTNwOUSxPFch|ryP Mm[zV2FPT0WU
JVM-2 M3PVRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTNwOUS3OVIh|ryP NHT5NmhUSU6JRWK=
EW-16 MoHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfiOmxYUUN3ME2zMlk2PTd5IN88US=> M2OwSXNCVkeHUh?=
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RCC10RGB NITDXpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVL4SFl2UUN3ME20MlAyOjR4IN88US=> MWXTRW5ITVJ?
TCCSUP MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rwUWlEPTB;ND6xNVA2KM7:TR?= MVXTRW5ITVJ?
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LU-139 NHm4[WlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXzTVZKSzVyPUSuN|czPDdizszN MVzTRW5ITVJ?
HCC1937 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPtTWM2OD12LkO3PVk{KM7:TR?= NGfvTHdUSU6JRWK=
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SK-OV-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLkTWM2OD12LkS2NFQzKM7:TR?= MlvwV2FPT0WU
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KYSE-520 M1vBPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPHfIJzUUN3ME20Mlg5ODF6IN88US=> MUjTRW5ITVJ?
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KOSC-2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTBTWM2OD12Lkm5PVU6KM7:TR?= MVPTRW5ITVJ?
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RCM-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTZwMEe3NVkh|ryP M2HoUXNCVkeHUh?=
Mewo NGmxepNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHSTWM2OD14LkGxOFg4KM7:TR?= NYXnPFZFW0GQR1XS
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VA-ES-BJ MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVf2V2JOUUN3ME22MlMxPDZ{IN88US=> NHHrfFdUSU6JRWK=
CAL-120 NYjkWWI1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\KNmlEPTB;Nj60OVE3OiEQvF2= MV7TRW5ITVJ?
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GCT Ml;QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3T4cGlEPTB;Nj61N|M5PyEQvF2= M2XrSnNCVkeHUh?=
SH-4 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XVVGlEPTB;Nj61PFg1PCEQvF2= MnLxV2FPT0WU
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NB12 Ml25S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYflSGtrUUN3ME22Mlc5PDB2IN88US=> Mo\XV2FPT0WU
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TE-11 NYnJR4wzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;ROWlEPTB;Nz6wOFk3PyEQvF2= M2jPSXNCVkeHUh?=
NCI-H2405 MnrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTdwMEizPVMh|ryP NITCS29USU6JRWK=
OVCAR-4 M2fsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjmO4xKSzVyPUeuNVU{PSEQvF2= M{j3N3NCVkeHUh?=
KP-N-YS M3HTeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LWV2lEPTB;Nz6xPFE1KM7:TR?= NGfRcmdUSU6JRWK=
NOS-1 NVW3[oVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1ywUmlEPTB;Nz6yOVgzQCEQvF2= NETlXVlUSU6JRWK=
DMS-114 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\6cVRKSzVyPUeuNlk3OzFizszN NEn1VJNUSU6JRWK=
NCI-H2030 NXz3OFBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXzTWM2OD15LkOzOlk5KM7:TR?= NInsU4RUSU6JRWK=
HEL MlPzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\QTWM2OD15LkO3OVIyKM7:TR?= MXzTRW5ITVJ?
CAL-62 Ml;rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzwXo9xUUN3ME23MlM6QDZ4IN88US=> MkjsV2FPT0WU
COLO-668 M1v5Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTWTWM2OD15LkSzPVY4KM7:TR?= NYDGc3RZW0GQR1XS
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T98G NXXhfVFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTN5Lki2N|Uh|ryP MULTRW5ITVJ?
NB14 MnvWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTN6LkS1OFQh|ryP NXvJPFNwW0GQR1XS
D-423MG M37RZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PFTmlEPTB;M{iuOFg3QSEQvF2= MUnTRW5ITVJ?
NCI-H510A MoHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXF[|lRUUN3ME2zPE43PDVzIN88US=> NFy0[29USU6JRWK=
NMC-G1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfBV4JKSzVyPUO4MlczOjJizszN MmniV2FPT0WU
SW948 MnXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\M[JdKSzVyPUO4Mlg6PDlizszN MV\TRW5ITVJ?
MFM-223 NVnST2NyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPmTWM2OD1|OD65OFMyKM7:TR?= NYDjS5B7W0GQR1XS
PAN1 NYfsPHJtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjoTWM2OD1|OD65PVE6KM7:TR?= NUPTeVljW0GQR1XS
MZ7-mel NWG4ZnptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nUNWlEPTB;M{muNVE6QSEQvF2= MUHTRW5ITVJ?
COLO-679 MkXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG[4fIZKSzVyPUO5Mlk4ODhizszN M{TmZnNCVkeHUh?=
SW872 NV3XUlBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTRyLkKwN|Eh|ryP NETNVG9USU6JRWK=
HT-1197 NVPv[XVIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTRyLkK4Olkh|ryP NGjtTplUSU6JRWK=
ES6 M{\M[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTRyLkS1OVIh|ryP NWXhVlJmW0GQR1XS
SNU-387 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\HW3VKSzVyPUSwMlgzOzhizszN MVzTRW5ITVJ?
T47D NYfzO2p7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELmOVJKSzVyPUSxMlAyQTJizszN MkK2V2FPT0WU
G-402 NETwOZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TIRmlEPTB;NEGuNFgxQSEQvF2= NULIc4VmW0GQR1XS
MZ1-PC MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\5TWM2OD12MT64NUDPxE1? MX3TRW5ITVJ?
SW1417 NFu4[nJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4G1UGlEPTB;NEKuN|A{KM7:TR?= Mo\5V2FPT0WU
RERF-LC-MS NYnzV2ptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;JdppYUUN3ME20Nk42ODh5IN88US=> MoDyV2FPT0WU
SF539 NWLtZ29vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nMfWlEPTB;NEKuOVUzQSEQvF2= MX7TRW5ITVJ?
ESS-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\PT4JKSzVyPUSzMlU2PThizszN NU\td2JCW0GQR1XS
RO82-W-1 NVzHSJhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DwfWlEPTB;NEOuOlE2OyEQvF2= NUDzNpJHW0GQR1XS
S-117 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHzT3dKSzVyPUSzMlY6PThizszN NFrPNGpUSU6JRWK=
SW756 Mk\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHnTWM2OD12Mz64NlQzKM7:TR?= NXPLfFZZW0GQR1XS
CP66-MEL MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzv[GVKSzVyPUS1MlcyPDNizszN NE\YWopUSU6JRWK=
H-EMC-SS NEfQbpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTR3Lke2PVMh|ryP MnXRV2FPT0WU
NCI-H2052 NWraT5g3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTR3LkmwNlch|ryP NVv4RVdCW0GQR1XS
SW684 M3\DSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjV[WRFUUN3ME20O{4xODhzIN88US=> Mly1V2FPT0WU
HCC1569 NHXwWppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDpXo5KSzVyPUS3MlE3PThizszN MVHTRW5ITVJ?
KGN NHPHbYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\XPGlEPTB;NEeuN|MyOSEQvF2= M{Hob3NCVkeHUh?=
LCLC-103H NIPyNVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTtbFd3UUN3ME20O{41PDB2IN88US=> Ml3kV2FPT0WU
SW780 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XFOGlEPTB;NEeuOlM2OSEQvF2= MV\TRW5ITVJ?
NCI-H1703 Mlm5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nB[2lEPTB;NEiuN|M2OiEQvF2= NEHCbmtUSU6JRWK=
HCC1395 NIPvV2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zMOmlEPTB;NEiuO|Q{OiEQvF2= M3\rN3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

体内研究 Gefitinib 作用于无胸腺裸鼠,抑制SKOV3 或MDA-MB-231 肿瘤生长。Gefitinib高效作用于两种类型肿瘤。处理14天后,MDA-MB-231肿瘤和SKOV3肿瘤生长抑制分别为71%和32%。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
+ 展开
  • Cell lines: NR6, NR6M 和 NR6W 细胞
  • Concentrations: 0 μM -2 μM
  • Incubation Time: 72 小时
  • Method: 呈指数生长的细胞,包括 NR6, NR6M, NR6M和NR6W细胞按6倍接种在96孔板上,每孔为2000个细胞, 粘附,随后在PBS 中清洗,然后在含0.5% FCS的培养基上温育过夜。使用不同浓度(0-2 μM) Gefitinib或溶质对照DMSO 和EGF 处理细胞。诱导NR6wtEGFR和NR6W 细胞增殖的EGF最佳浓度已经测定,因此NR6wtEGFR和 NR6W 细胞中分别加入10 nM和 0.1 nM EGF。NR6 和NR6M细胞中不加入EGF。72 小时后,通过MTT 增殖检测测量细胞数。
    (Only for Reference)
动物实验:[3]
+ 展开
  • Animal Models: 携带 SKOV3或MDA-MB-231肿瘤的无胸腺裸鼠
  • Formulation: 0.2% DMSO
  • Dosages: 75 mg/kg
  • Administration: 饲喂处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% CMC Na
30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 446.90
化学式

C22H24ClFN4O3

CAS号 184475-35-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03720873 Recruiting EGFR Gene Mutation Fujian Cancer Hospital October 2018 Phase 2
NCT03599518 Recruiting Non Small Cell Lung Cancer Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. October 10 2018 Phase 1
NCT03653546 Not yet recruiting Non-small Cell Lung Cancer|EGFR Gene Mutation|Brain Metastases Alpha Biopharma (Jiangsu) Co. Ltd. August 31 2018 Phase 2|Phase 3
NCT03602027 Not yet recruiting Non-squamous Non-small Cell Lung Cancer Second Affiliated Hospital of Nanchang University August 1 2018 Not Applicable
NCT03656393 Recruiting NSCLC|EGFR Shenzhen People''s Hospital August 31 2018 Phase 3
NCT03529084 Withdrawn Carcinoma Non-small Cell Lung Novartis Pharmaceuticals|Novartis July 23 2018 Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID