Gefitinib (ZD1839)

目录号:S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。

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客户使用Selleck该产品发表文献98篇:

客户使用该产品的14个实验数据:

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。
特性 Gefitinib是有效的EGFR酪氨酸激酶抑制剂。
靶点
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外研究

Gefitinib 作用于高和低表达EGFR的细胞系,包括NR6, NR6M 和 NR6W 细胞系,有效抑制EGFR上全部酪氨酸磷酸化位点。磷酸化位点 Tyr1173和Tyr992敏感性较低,需要高浓度Gefitinib才能抑制。Gefitinib作用于NR6W细胞,高效阻断PLC-γ磷酸化, IC50为27nM。NR6wtEGFR 和NR6M细胞系具有低水平PLC-γ磷酸化, IC50 分别为 43 nM和 369 nM。Gefitinib抑制AKT磷酸化,作用于低EGFR和EGFRvIII表达的细胞系, IC50 分别为220 和263nM。Gefitinib按 0.1 到 0.5μM剂量处理,显著促进而不是废除 NR6M细胞形成集落。然而, 2μM Gefitinib 完全抑制 NR6M 集落形成。Gefitinib 作用于高和低EGFR表达的细胞系,EGF 刺激72小时后,快速抑制EGFR 和ERK 磷酸化,这种作用存在剂量依赖性。[1]Gefitinib抑制 EGF驱动的未转化MCF10A 细胞生长,IC50为 20 nM。[2] Gefitinib 作用于MDA-MB-231细胞,抑制EGF信号。Gefitinib严重抑制细胞数的增多,这种作用存在剂量依赖性。 [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M1nNXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMEW0JO69VQ>? MVyxOlUyPjR5Mx?=
NIH3T3 NETBWI5HfW6ldHnvckBCe3OjeR?= MXKwMlIh|ryP M3uyUVIhcA>? MnjoSG1UVw>? MkDhTY5pcWKrdHnvckBw\iCHR1[gd5RqdXWuYYTl[EBpfW2jbjDldoJDOSCjdYTvdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAxNjBzNESg{txO NUHrd5JPOTZ2OECyPFQ>
COS7 MVvGeY5kfGmxbjDBd5NigQ>? MXS5OkDPxE1? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGpCUzJiVk[xO2YhdXW2YX70JIV5eHKnc4Pl[C=> NFrEfJcyPzF5OEeyNi=>
A549 NH3aWohEgXSxdH;4bYMhSXO|YYm= M2O5O|k3KGh? MXPEUXNQ MkCyTWM2OD1zMz61PUDPxE1? Ml\QNVg{OTN6MEe=
K562 NV72U5RwS3m2b4TvfIlkKEG|c3H5 MWe5OkBp MmfBSG1UVw>? M{Xmb2lEPTB;OT6zOkDPxE1? NGH5fJUyQDNzM{iwOy=>
MDA-MB-231 MU\DfZRwfG:6aXOgRZN{[Xl? MUm5OkBp NInVNYpFVVOR NWKzZYh1UUN3ME2zOk43OSEQvF2= NHXJVZMyQDNzM{iwOy=>
MOLT4 NUG0U3pES3m2b4TvfIlkKEG|c3H5 NWDlTXg1QTZiaB?= M1rnSmROW09? NF;rT2xKSzVyPUG1MlAzKM7:TR?= MknWNVg{OTN6MEe=
MCF7 Mkn6R5l1d3SxeHnjJGF{e2G7 NEP5Tos2OCEQvF2= MUe3NkBp Mk\uTWM2OD1zMj6wOUDPxE1? M1OwdVE5PzdzOEG5
BT474 M1XScGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jCeVczKGh? M17sT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS0O|Qh[2WubIOgc5ZmemW6cILld5NqdmdiRWLCZlIhf2m2aDDFR|UxKG:oIECuN{DPxE1? MYKxPVAzQDR{NB?=
HN5 NUjYd5cyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4L3XFczKGh? MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiQNTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghTUN3MDDv[kAxNjBzIN88US=> NFXtO3QyQTB{OESyOC=>
BA/F3 MnzpR5l1d3SxeHnjJGF{e2G7 MXq3NkBp NYjzW48xS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? M4izOlE6OjN7MkK5
NIH3T3 M2rqOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL0UJRPPzJiaB?= MknSS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> Mk\wNVk3PjV|N{e=
MCF7 MX Mnu5SpVv[3Srb36gRZN{[Xl? MXKzNEBucW5? M37weGlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhf2m2aDDJR|UxKG:oIEGuNUDPxE1? NF\wSJoyQTl|Mkm2NC=>
A431 NUnMU5NST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrZfYYyOCEQvF2= NV7Eb|JpPzJiaB?= NWXKXGgyT0l3ME2wMlUyPCEQvF2= MXSxPVk3QTR4NR?=
A431 NVXNd2N5U2mwYYPlJGF{e2G7 M{fsNFAvOSEQvF2= Ml;YNUBp MXTJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u NEiyU4gyQTl4OUS2OS=>
A431 NXvOXFZkU2mwYYPlJGF{e2G7 MXWxNFAh|ryP MnrFNVAhdWmw MmLwVJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> MXOxPVk3QTR4NR?=
Sf9 M3nURWtqdmG|ZTDBd5NigQ>? NYf4bIR5OTBibXnu M1u5XWROW09? M1q3b2lvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= NU\kTXQ{OjByNU[0NlU>
NCI-H1975 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NED2U5Q2KM7:TR?= NXPnfIk2PzJiaB?= M1\mUWlEPTB;OD6yOkDPxE1? NVT1SlQ5OjBzNUG2O|A>
NCI-H1975 NH3odFBMcW6jc3WgRZN{[Xl? NYP0dJhJOTBizszN NEnXWmdKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? MXSyNFE2OTZ5MB?=
NCI-H1975 MY\GeY5kfGmxbjDBd5NigQ>? MX:xNEDPxE1? MnzkTY5pcWKrdHnvckBw\iCHUlLCNkBifXSxcHjvd5Bpd3K7bHH0bY9v Ml\xNlAyPTF4N{C=
NCI-H1975 NFvzc41HfW6ldHnvckBCe3OjeR?= Mnn5OUDPxE1? NVXidHpSPDhiaB?= NWi5PGpIUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2VvMx?= MV:yNFE2OTZ5MB?=
A375 M4nzd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2f4TFQ5KGh? MYjJR|UxRTJyLkW1JO69VQ>? NIrkWXQzODNyNEWzOy=>
SMMC7721 MkL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVi0PEBp MX7JR|UxRTN|LkGyJO69VQ>? MmXKNlA{ODR3M{e=
DU145 NYLFRXRyS3m2b4TvfIlkKEG|c3H5 M1LhXFUh\A>? NVLSd|RWTE2VTx?= MmLRTWM2OD12Mj6xO{DPxE1? NEDtXJMzODR4NkW1OS=>
MIAPaCa2 NVTre5ZJS3m2b4TvfIlkKEG|c3H5 M{Py[lUh\A>? M2frfGROW09? NIDpXWhKSzVyPUWwMlYzKM7:TR?= MWKyNFQ3PjV3NR?=
PANC1 NVTmXG5bS3m2b4TvfIlkKEG|c3H5 M1vRVlUh\A>? M{OydmROW09? NXTJbGw1UUN3ME20NE41OiEQvF2= MoXMNlA1PjZ3NUW=
PC9 NFXUVldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlK3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NHS1SYkzOTJyOEiwNi=>
PC9 M37YO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= NEfu[2gzOTJyOEiwNi=>
PC3 NUfaUpUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3yU3hzOTBizszN MX:3NkBp NW\ibXF[TE2VTx?= NWLkbYR{UUN3ME23MlQh|ryP MnTWNlE{PTN3NE[=
MDA-MB-468 NH:0fXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWqxNFAh|ryP NWjrS2lPPDhiaB?= NIP6PGlFVVOR MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= NWKwOVVSOjF2Mkm2N|I>
Plasmodium falciparum NWPSd25QeUiWUzDwdo9ncWyrbneg[o9zKGmwaHnibZRwenNib3[gVIxie22xZHn1cUBn[WylaYDhdpVuKHC{b3zp[oVz[XSrb36= M3XPVlIyQDF5MES1
NCI-H1993 M4LLWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnYZ4c4OiCq NY\VPVR4TE2VTx?= MoWxTWM2OD1yLkGg{txO MUKyNVkyPjR|Mx?=
NCI-H292 NX;1bHJvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;TTlczKGh? MV\EUXNQ NUHsU5JYUUN3ME2wMlAzKM7:TR?= MXmyNVkyPjR|Mx?=
A431 MXPLbY5ie2ViQYPzZZk> MYKyJIg> NI[5NndFVVOR M{HvOWlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36gbY4hTUeILYP0bY12dGG2ZXSgbJVu[W5iQUSzNUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP NIH0NnozOjFyMUGzNi=>
MCF7 NGHNc|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrYNW8yODEEoN88[{9uVA>? NHjPWm01QCCq M3n5bWROW09? NHTLVGNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> MlG4NlIyOTlzM{C=
BT474 M3XqSGN6fG:2b4jpZ{BCe3OjeR?= NXrZUHV[OTBizszN NXfrenRXPDhiaB?= MYrDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| MXyyNlE{QDNyOB?=
MCF7 MlnHR5l1d3SxeHnjJGF{e2G7 NYjOOYt{OTBizszN MWW0PEBp M320WWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= MWCyNlE{QDNyOB?=
HCC827 M1XKd2tqdmG|ZTDBd5NigQ>? NGrSd3ExNjF{NTFOwG0> NGmwdFAzPCCq NYHOfZVmTE2VTx?= M4HCc2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MWGyNlM{QTN2Mh?=
HCC827 MmPwT4lv[XOnIFHzd4F6 NUL5UmxJOC5zMkWg{txO M1XoW|I1KGh? MXrEUXNQ MYfJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhTXKtIIDoc5NxcG:{eXzheIlwdg>? NWPWR5JCOjJ|M{mzOFI>
HCC827 MX;LbY5ie2ViQYPzZZk> MUmwMlEzPSEQvF2= MYGyOEBp MmjhSG1UVw>? NYL1SnFIUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= NVn6WpBkOjJ|M{mzOFI>
A431 NH7JV41McW6jc3WgRZN{[Xl? MX2xNFAh|ryP NFP3SGI{OCCvaX6= NHjyfGxKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN NIfCdGwzOjZ7OEe4Ni=>
H460 M{jGNmN6fG:2b4jpZ{BCe3OjeR?= M3XDU|czKGh? NV;rTHY6UUN3ME21MlU6KM7:TR?= M4\UcFIzPzJ5NES5
HT-29 M1z2NGN6fG:2b4jpZ{BCe3OjeR?= MX63NkBp NHjpbJNKSzVyPUOuN|Yh|ryP NHO2Z3czOjd{N{S0PS=>
SGC7901 MlPTR5l1d3SxeHnjJGF{e2G7 MYm3NkBp M3LBcGlEPTB;MUCuNlYh|ryP MkXPNlI4Ojd2NEm=
A498 NHrWOIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmSyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNEm4JINmdGy|IHX4dJJme3OrbnegSWdHWiCjbnSgV3JEKHerdHigTWM2OCCxZjC4MlYh|ryP MVSyNlgyQDh2OB?=
Calu3 NVXkeIE{S3m2b4TvfIlkKEG|c3H5 MWCxNEDPxGdxbVy= NFLmRlY4OiCq M{TjbmlEPTB;MT62JO69VQ>? NVGzWmFlOjNzMU[xOlg>
FADU M2Tje2N6fG:2b4jpZ{BCe3OjeR?= M2XCdlExKM7:Zz;tUC=> M3LPR|czKGh? NWrjWJNJUUN3ME2wMlA2KM7:TR?= M3P5PVI{OTF4MU[4
HCC827 MknXR5l1d3SxeHnjJGF{e2G7 MnLwNVAh|rypL33M M2DWUlczKGh? MnHlTWM2OD1yLkCwNVUh|ryP NYLnZpozOjNzMU[xOlg>
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SCC-4 MlXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTJwMkG3O|Yh|ryP MlHHV2FPT0WU
BT-20 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTFTWM2OD1{LkK2O|Q1KM7:TR?= MUHTRW5ITVJ?
A498 MkDGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7JcIczUUN3ME2yMlI6PDF3IN88US=> Mn;RV2FPT0WU
IGROV-1 NGTuUodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fjPGlEPTB;Mj6zNVI{PyEQvF2= M{PGcHNCVkeHUh?=
JVM-3 NXnLT|k1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWW3VWVFUUN3ME2yMlM1PjR|IN88US=> MWLTRW5ITVJ?
NCI-H2170 NYXKZYNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTJwM{S3PFMh|ryP MkPPV2FPT0WU
EW-18 MmPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17rdWlEPTB;Mj6zOVQ4OSEQvF2= M4[xPHNCVkeHUh?=
KU812 NHnS[2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTJwM{[3O|kh|ryP MnjwV2FPT0WU
NY NF;0SVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnNTWM2OD1{LkO3Nlch|ryP M1j6[3NCVkeHUh?=
HTC-C3 NIDGU2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjhOnhKSzVyPUKuOFIzPjdizszN NFvTNFJUSU6JRWK=
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TE-8 NUjzVGRoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TtRWlEPTB;Mj62N|Y2OyEQvF2= MoP1V2FPT0WU
MC-IXC MmTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fjcWlEPTB;Mj62OFQ2OyEQvF2= NGfHbIlUSU6JRWK=
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Detroit562 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LycWlEPTB;Mj62PVg1PSEQvF2= NFTYNYhUSU6JRWK=
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QIMR-WIL NHK1OWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrmTWM2OD1{Lke1PVUzKM7:TR?= MlfoV2FPT0WU
BB65-RCC NYXPO29oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrmWo5KSzVyPUKuPFA4OTNizszN MVvTRW5ITVJ?
PC-3 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mli2TWM2OD1{LkizN|E4KM7:TR?= NIf1[o1USU6JRWK=
OVCAR-3 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\CTWM2OD1{Lki1OVk1KM7:TR?= M4TIW3NCVkeHUh?=
KYSE-510 NGXFUmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPHTWM2OD1{Lki2O|g3KM7:TR?= NXvsXotNW0GQR1XS
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NCI-H1693 Mo\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIn2S3pKSzVyPUKuPVU5QTdizszN NVnEbXlbW0GQR1XS
DU-4475 NH3PR|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnS1TWM2OD1{Lkm5NFUzKM7:TR?= NWjo[nQ5W0GQR1XS
COLO-680N MlqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfUVlFGUUN3ME2zMlAxPDl3IN88US=> MX3TRW5ITVJ?
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HT-1376 NVy5Nnc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF21Z5hKSzVyPUOuNlA2ODZizszN MUDTRW5ITVJ?
NCI-H1793 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjxfVRKSzVyPUOuNlExQThizszN M{DMUHNCVkeHUh?=
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NCI-H727 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTNwM{i1PFQh|ryP Mo\UV2FPT0WU
LB771-HNC NV3jXIlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTNwM{mxNlYh|ryP Mme5V2FPT0WU
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639-V MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnO5TWM2OD1|LkW3O|kzKM7:TR?= MmDMV2FPT0WU
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SK-LU-1 M3e5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrPSmdKSzVyPUOuOlA3PDVizszN M1vpV3NCVkeHUh?=
CaR-1 NGTh[GNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojZTWM2OD1|Lk[xNFQ5KM7:TR?= M4DNOnNCVkeHUh?=
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SK-MES-1 NH7YeGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrnSVExUUN3ME2zMlY1PjF2IN88US=> MojmV2FPT0WU
PANC-10-05 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrZdXR2UUN3ME2zMlczODd{IN88US=> M1\SbnNCVkeHUh?=
J-RT3-T3-5 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTNwN{W0NFYh|ryP MoC5V2FPT0WU
ES4 M1ziZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnGxTWM2OD1|LkiyNlg3KM7:TR?= NGPXOFdUSU6JRWK=
NCI-H28 MnfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\GeWlEPTB;Mz64Nlk3OSEQvF2= NFT4UXpUSU6JRWK=
NCI-H1155 MmHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTNwOESxOVkh|ryP NF;uN5hUSU6JRWK=
KURAMOCHI MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkf1TWM2OD1|Lki2NFU3KM7:TR?= MXHTRW5ITVJ?
NCI-H69 NUWxNY5WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjRTWM2OD1|LkmwNVc5KM7:TR?= MWTTRW5ITVJ?
IST-MES1 MkXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\FdYdSUUN3ME2zMlk1ODJ7IN88US=> M3qycXNCVkeHUh?=
NH-12 MlXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfENnpbUUN3ME2zMlk1OTh5IN88US=> NXvWemZNW0GQR1XS
JVM-2 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXSPHdKSzVyPUOuPVQ4PTJizszN MlXtV2FPT0WU
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NCI-H441 MonkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LjSmlEPTB;Mz65O|AyPCEQvF2= M1z3NHNCVkeHUh?=
RCC10RGB MlGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTRwMEGyOFYh|ryP M1XGWnNCVkeHUh?=
TCCSUP M1fx[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzwcm9KSzVyPUSuNVExPSEQvF2= NUHxNWJrW0GQR1XS
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KARPAS-299 NYrxN5NYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jHOWlEPTB;ND6zNVAyOyEQvF2= NX36d4JtW0GQR1XS
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LU-139 NXL3ZVZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mne4TWM2OD12LkO3NlQ4KM7:TR?= MlrkV2FPT0WU
HCC1937 M2LMSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfWTWM2OD12LkO3PVk{KM7:TR?= MUfTRW5ITVJ?
GR-ST M17Md2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\Ob5NKSzVyPUSuOFMyQCEQvF2= NFqyR4VUSU6JRWK=
SK-OV-3 M2LId2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGizSXNKSzVyPUSuOFYxPDJizszN MYrTRW5ITVJ?
A172 NVfHflRET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIq4TXFKSzVyPUSuOVU2OjFizszN NHTnUm9USU6JRWK=
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P30-OHK M1TKOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zEfGlEPTB;ND64NVkzQSEQvF2= MYPTRW5ITVJ?
KYSE-520 M2HqN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\GTWM2OD12Lki4NFE5KM7:TR?= NV23XFl3W0GQR1XS
KNS-62 NH3vUolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPUTWM2OD12LkmwPFA4KM7:TR?= MofZV2FPT0WU
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KOSC-2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTRwOUm5OVkh|ryP NVPUTW5xW0GQR1XS
TE-9 NFLvTnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXHcYN5UUN3ME21MlAyPTJ3IN88US=> MX\TRW5ITVJ?
NCI-H1838 M161[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\HOmZKSzVyPUWuNFIxPzZizszN MnHnV2FPT0WU
KP-N-YN NUXONIVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jveGlEPTB;NT6xNlU6OSEQvF2= MkfaV2FPT0WU
ALL-PO NGT5VpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3r2VmlEPTB;NT6yNlU5PyEQvF2= NXfWN3l4W0GQR1XS
NOMO-1 MnfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULaeYw4UUN3ME21MlI{OTd4IN88US=> MUjTRW5ITVJ?
CGTH-W-1 NXfGb3U1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjDZ21KSzVyPUWuN|g2QDRizszN M2fWTHNCVkeHUh?=
ATN-1 NFrOWWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETKbHdKSzVyPUWuOFEyQDRizszN M1v4WnNCVkeHUh?=
AGS M2rURWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;MNm1KSzVyPUWuOFk3OThizszN NHXFSIlUSU6JRWK=
SW13 NI\LTnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTVwNUOyOlUh|ryP NFH4dFhUSU6JRWK=
SW1990 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7QXmNKSzVyPUWuOlIzQDVizszN MX7TRW5ITVJ?
BFTC-905 NUjyVIZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrvOHlwUUN3ME21MlY2QDd6IN88US=> NEixWmFUSU6JRWK=
RMG-I MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvUTWM2OD13Lk[1PVQzKM7:TR?= NYrQenRiW0GQR1XS
HD-MY-Z M3jxc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHWzNIJKSzVyPUWuOlg2QDFizszN NXSzSFFZW0GQR1XS
HCC1187 M4jJS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrsR5I4UUN3ME21MlcyODN{IN88US=> MUXTRW5ITVJ?
HCC2998 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmiwTWM2OD13LkexPFc4KM7:TR?= Mn;BV2FPT0WU
RT-112 NUPSTVdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PTRWlEPTB;NT65NFI1PSEQvF2= M1vjd3NCVkeHUh?=
CAKI-1 NF;We2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHkTFc3UUN3ME22MlAxQTh3IN88US=> MYfTRW5ITVJ?
HDLM-2 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnv1TWM2OD14LkCxOFg6KM7:TR?= MkCzV2FPT0WU
TE-10 NX75dldIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17ERWlEPTB;Nj6wOFg{OyEQvF2= NWLPWGRrW0GQR1XS
KE-37 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLKWolOUUN3ME22MlA4OjV6IN88US=> NYq1fYVMW0GQR1XS
RCM-1 MmDRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDYc5RKSzVyPU[uNFc4OTlizszN MVTTRW5ITVJ?
Mewo MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\mTWM2OD14LkGxOFg4KM7:TR?= MWjTRW5ITVJ?
L-428 NXrnTXVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTZwMUiwN|ch|ryP M{K4ZXNCVkeHUh?=
8-MG-BA M2C0Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDIV5ZKSzVyPU[uNVk{PzlizszN NHfZcm1USU6JRWK=
SK-MEL-28 NFfl[nRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkS2TWM2OD14LkKwPVY4KM7:TR?= NV\MfGtXW0GQR1XS
VA-ES-BJ M{HXNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;6e2NKSzVyPU[uN|A1PjJizszN Ml7KV2FPT0WU
CAL-120 Mn7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTZwNEWxOlIh|ryP MmjSV2FPT0WU
GAMG MorJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfqTWM2OD14LkWwOlIh|ryP NGrFcmxUSU6JRWK=
MSTO-221H NIXrUXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2n4SGlEPTB;Nj61NVE4KM7:TR?= NHq1VGtUSU6JRWK=
GCT NHmzcmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETUZlhKSzVyPU[uOVM{QDdizszN MlfMV2FPT0WU
SH-4 M2CwUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTZwNUi4OFQh|ryP MXrTRW5ITVJ?
Calu-3 M3O0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnxNmRKSzVyPU[uOlU2PjhizszN M1jqZnNCVkeHUh?=
SK-HEP-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTZwN{G3OVMh|ryP M37aT3NCVkeHUh?=
PFSK-1 MmnwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvWe3hKSzVyPU[uO|M1PzhizszN MYfTRW5ITVJ?
NB12 M4D2TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXFTWM2OD14Lke4OFA1KM7:TR?= MUnTRW5ITVJ?
A388 NH7RcodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPKU29DUUN3ME22Mlg6PTJizszN MkHNV2FPT0WU
KLE M1y3fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrMT5ZKSzVyPU[uPVY2OTZizszN NUCyW2FXW0GQR1XS
TE-11 M3e0Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTdwMES5Olch|ryP NULyTIJTW0GQR1XS
NCI-H2405 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;nTWM2OD15LkC4N|k{KM7:TR?= M{n5SXNCVkeHUh?=
OVCAR-4 NYnoPGRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWSxT2hOUUN3ME23MlE2OzVizszN NXXqXJF1W0GQR1XS
KP-N-YS NGPFXlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XQZ2lEPTB;Nz6xPFE1KM7:TR?= NWTUSYVVW0GQR1XS
NOS-1 NF\pdVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlf4TWM2OD15LkK1PFI5KM7:TR?= Mk\TV2FPT0WU
DMS-114 M1HOVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjPTJJ4UUN3ME23MlI6PjNzIN88US=> MmnaV2FPT0WU
NCI-H2030 MkTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HFcmlEPTB;Nz6zN|Y6QCEQvF2= MVfTRW5ITVJ?
HEL MoPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3KTWM2OD15LkO3OVIyKM7:TR?= MoruV2FPT0WU
CAL-62 NYXhWWp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\HVWl5UUN3ME23MlM6QDZ4IN88US=> NHTJ[2ZUSU6JRWK=
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SKG-IIIa Mk\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTdwNEWzPFEh|ryP MXvTRW5ITVJ?
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SW626 NF[yS4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7wVJU6UUN3ME2zO{4xPDF5IN88US=> NYDIdZI5W0GQR1XS
ONS-76 NWHJUnVPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\oTWM2OD1|Nz6xN|A6KM7:TR?= NWjjUHNoW0GQR1XS
T98G MnjjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXkTWFKSzVyPUO3Mlg3OzVizszN M4\KRXNCVkeHUh?=
NB14 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rMe2lEPTB;M{iuOFU1PCEQvF2= NH3kR3pUSU6JRWK=
D-423MG MnrGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV34NJdQUUN3ME2zPE41QDZ7IN88US=> NVzyUYtEW0GQR1XS
NCI-H510A NFnDc21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTN6Lk[0OVEh|ryP NFWyUmFUSU6JRWK=
NMC-G1 M{XV[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2D6VmlEPTB;M{iuO|IzOiEQvF2= MknhV2FPT0WU
SW948 M3fGfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfpNFJKSzVyPUO4Mlg6PDlizszN MWDTRW5ITVJ?
MFM-223 NXXw[Gp6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTN6Lkm0N|Eh|ryP MVTTRW5ITVJ?
PAN1 NX6xU4JTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjPeVNKSzVyPUO4Mlk6OTlizszN NFTqRoFUSU6JRWK=
MZ7-mel Mnj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHe3OJRKSzVyPUO5MlEyQTlizszN M1e2TXNCVkeHUh?=
COLO-679 M4nNcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TUPWlEPTB;M{muPVcxQCEQvF2= NF3PZ5dUSU6JRWK=
SW872 MnPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfBdnlGUUN3ME20NE4zODNzIN88US=> MXLTRW5ITVJ?
HT-1197 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\DPGlEPTB;NECuNlg3QSEQvF2= NGHkSnNUSU6JRWK=
ES6 NFPHNGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rtVGlEPTB;NECuOFU2OiEQvF2= NWPiUZlqW0GQR1XS
SNU-387 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\OeY5oUUN3ME20NE45OjN6IN88US=> Ml;tV2FPT0WU
T47D MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DYVGlEPTB;NEGuNFE6OiEQvF2= M3G4R3NCVkeHUh?=
G-402 MorTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHXVJl[UUN3ME20NU4xQDB7IN88US=> NUHFbmZZW0GQR1XS
MZ1-PC M4j2cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTRzLkixJO69VQ>? MmDhV2FPT0WU
SW1417 NFXxWYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2L0NmlEPTB;NEKuN|A{KM7:TR?= MkO1V2FPT0WU
RERF-LC-MS NW\WTZBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEezdnZKSzVyPUSyMlUxQDdizszN MlvyV2FPT0WU
SF539 NHzjW5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVToSYxvUUN3ME20Nk42PTJ7IN88US=> MYrTRW5ITVJ?
ESS-1 MoX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnwWnNWUUN3ME20N{42PTV6IN88US=> NIjMZYtUSU6JRWK=
RO82-W-1 M164c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTR|Lk[xOVMh|ryP MYnTRW5ITVJ?
S-117 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHTTWM2OD12Mz62PVU5KM7:TR?= MmOxV2FPT0WU
SW756 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Oz[2lEPTB;NEOuPFI1OiEQvF2= M4\wOnNCVkeHUh?=
CP66-MEL MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LofGlEPTB;NEWuO|E1OyEQvF2= NGrKc4lUSU6JRWK=
H-EMC-SS Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrBNVdKSzVyPUS1Mlc3QTNizszN MnG3V2FPT0WU
NCI-H2052 M1fHVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLaZ3NKSzVyPUS1MlkxOjdizszN MojxV2FPT0WU
SW684 NX3F[Yt4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLSTWM2OD12Nz6wNFgyKM7:TR?= NYfFdnk{W0GQR1XS
HCC1569 Mn7mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TEXGlEPTB;NEeuNVY2QCEQvF2= MYPTRW5ITVJ?
KGN NXfiboFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3q3SmlEPTB;NEeuN|MyOSEQvF2= MV;TRW5ITVJ?
LCLC-103H MnztS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\0TWM2OD12Nz60OFA1KM7:TR?= MWLTRW5ITVJ?
SW780 MoXnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrKTWM2OD12Nz62N|UyKM7:TR?= MlfnV2FPT0WU
NCI-H1703 MorPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTR6LkOzOVIh|ryP NELUNWFUSU6JRWK=
HCC1395 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTR6Lke0N|Ih|ryP NHP0TIxUSU6JRWK=

... Click to View More Cell Line Experimental Data

体内研究 Gefitinib 作用于无胸腺裸鼠,抑制SKOV3 或MDA-MB-231 肿瘤生长。Gefitinib高效作用于两种类型肿瘤。处理14天后,MDA-MB-231肿瘤和SKOV3肿瘤生长抑制分别为71%和32%。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
+ 展开
  • Cell lines: NR6, NR6M 和 NR6W 细胞
  • Concentrations: 0 μM -2 μM
  • Incubation Time: 72 小时
  • Method: 呈指数生长的细胞,包括 NR6, NR6M, NR6M和NR6W细胞按6倍接种在96孔板上,每孔为2000个细胞, 粘附,随后在PBS 中清洗,然后在含0.5% FCS的培养基上温育过夜。使用不同浓度(0-2 μM) Gefitinib或溶质对照DMSO 和EGF 处理细胞。诱导NR6wtEGFR和NR6W 细胞增殖的EGF最佳浓度已经测定,因此NR6wtEGFR和 NR6W 细胞中分别加入10 nM和 0.1 nM EGF。NR6 和NR6M细胞中不加入EGF。72 小时后,通过MTT 增殖检测测量细胞数。
    (Only for Reference)
动物实验:[3]
+ 展开
  • Animal Models: 携带 SKOV3或MDA-MB-231肿瘤的无胸腺裸鼠
  • Formulation: 0.2% DMSO
  • Dosages: 75 mg/kg
  • Administration: 饲喂处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% CMC Na
30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 446.90
化学式

C22H24ClFN4O3

CAS号 184475-35-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03766490 Not yet recruiting Non-Small-Cell Lung The First Affiliated Hospital of Soochow University|The First People''s Hospital of Changzhou|Second Affiliated Hospital of Soochow University|Nantong University|Affiliated Hospital of Jiangnan University|Jiangyin People''s Hospital|Changzhou No.2 People''s Hospital|Chia Tai Tianqing Pharmaceutical Group Co. Ltd. March 30 2019 Not Applicable
NCT03856697 Not yet recruiting Advanced Non-small Cell Lung Cancer Hangzhou ACEA Pharmaceutical Research Co. Ltd.|Guangdong Provincial People''s Hospital March 2019 Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID