Vandetanib (ZD6474)

For research use only. Not for use in humans.

目录号:S1046 中文名称:凡德他尼

Vandetanib (ZD6474) Chemical Structure

CAS No. 443913-73-3

Vandetanib (ZD6474) 是一种有效的 VEGFR2 抑制剂,在无细胞试验中IC50为40 nM。同时,也抑制VEGFR3EGFR,IC50分别为110 nM 和500 nM。对PDGFRβ, Flt-1, Tie-2 和FGFR1作用效果不大,IC50为 1.1-3.6 μM, 对MEK, CDK2, c-Kit, erbB2, FAK, PDK1, Akt 和 IGF-1R几乎没有作用效果,IC50>10 μM。Vandetanib (ZD6474) 可增加凋亡并通过提高 reactive oxygen species (ROS) 的水平来诱导自噬。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1096.56 现货
RMB 1211.82 现货
RMB 2104.83 现货
RMB 3835.98 现货
RMB 5468.77 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Vandetanib (ZD6474)发表文献89篇:

产品安全说明书

VEGFR抑制剂选择性比较

生物活性

产品描述 Vandetanib (ZD6474) 是一种有效的 VEGFR2 抑制剂,在无细胞试验中IC50为40 nM。同时,也抑制VEGFR3EGFR,IC50分别为110 nM 和500 nM。对PDGFRβ, Flt-1, Tie-2 和FGFR1作用效果不大,IC50为 1.1-3.6 μM, 对MEK, CDK2, c-Kit, erbB2, FAK, PDK1, Akt 和 IGF-1R几乎没有作用效果,IC50>10 μM。Vandetanib (ZD6474) 可增加凋亡并通过提高 reactive oxygen species (ROS) 的水平来诱导自噬。
靶点
VEGFR2 [1]
(Cell-free assay)
VEGFR3 [1]
(Cell-free assay)
EGFR [1]
(Cell-free assay)
40 nM 110 nM 500 nM
体外研究

Vandetanib 也抑制VEGFR3和EGFR,IC50分别为110 nM 和500 nM。Vandetanib 对PDGFRβ, Flt-1, Tie-2 和FGFR1作用效果不大,IC50为 1.1-3.6 μM, 而对MEK, CDK2, c-Kit, erbB2, FAK, PDK1, Akt 和 IGF-1R几乎没有作用效果,IC50 > 10 μM。Vandetanib 抑制VEGF-, EGF- 和 bFGF-刺激的 HUVEC 增殖,IC50分别为60 nM, 170 nM 和 800 nM, 而对基底内皮细胞生长几乎没有作用效果。Vandetanib 抑制肿瘤细胞生长,IC50 为 2.7 μM (A549) 到13.5 μM (Calu-6)。[1] Vandetanib抑制basal ABCG2-ATP酶。亲本和表达ABCG2的A431细胞对 Vandetanib具有相似的敏感性。EGFR 抑制剂处理A431细胞,降低pEGFR水平,而Vandetanib 处理时,抑制效果温和。Gefitinib, Pelitinib 和 Neratinib完全抑制 ABCG2调节的Mitoxantrone从 A431/ABCG2细胞中外排,而Vandetanib只具有微弱且不可测量的抑制效果,与 特点ABCG2抑制剂Ko143类似。[2] Vandetanib抑制PC3wt 和PC3R细胞系,IC50分别为13.3 μM和11.5 μM。[3] Vandetanib 作用于HUVEC,抑制VEGFR-2磷酸化,作用于肝癌细胞,抑制EGFR磷酸化,且抑制细胞增殖。[4] Vandetanib 作用于GEO 和 OVCAR-3 细胞,使细胞在G0-G1期累积,作用于OVCAR-3, ZR-75-1, MCF-10A ras, 和 GEO 细胞,促进凋亡。Vandetanib 作用于小鼠NIH-EGFR 成纤维细胞和人类 MCF-10A ras 乳腺癌细胞(这两种细胞都过量表达人类EGFR),抑制EGFR磷酸化,这种作用存在剂量依赖性。Vandetanib 作用于有功能性EGFR但是缺失VEGFR-2的人类细胞系 (乳腺,结肠,胃,和卵巢),抑制软琼脂生长,这种作用具有剂量依赖性。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SN179  NHHSXpFHfW6ldHnvckBCe3OjeR?= MYm1NFDjiImwTdMg MnjCNVYhcA>? MXXpcoNz\WG|ZYOgR3hEWjRiZYjwdoV{e2mxbjDzbYdvcW[rY3HueIx6 MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ5Nk[5NUc,OjV4N{[2PVE9N2F-
SN186 NV;Xbnk6TnWwY4Tpc44hSXO|YYm= NUnWfG5rPTBy4pEJcm3DqA>? MXWxOkBp NU[1c2lQcW6lcnXhd4V{KEO[Q2K0JIV5eHKnc4Ppc44he2mpbnnmbYNidnSueR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ5Nk[5NUc,OjV4N{[2PVE9N2F-
SN179  NVjEZpNFTnWwY4Tpc44hSXO|YYm= M1HEUFUxOOLCiX7NxsA> NVTieYZtOTZiaB?= Mn\Y[Y5p[W6lZYOgeIhmKEO[Q1yxNkBlcXKnY4Tl[EBucWe{YYTpc44> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ5Nk[5NUc,OjV4N{[2PVE9N2F-
SN179  MXPGeY5kfGmxbjDBd5NigQ>? MV61NFDjiImwTdMg M{TvblE3KGh? M3OxbYlv[3KnYYPld{Bj[XOjbDDtbYdz[XSrb39CpC=> MkTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4N{[2PVEoRjJ3Nke2OlkyRC:jPh?=
Jurkat NIDOdXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWK3NuKhcMLi MnG4S2k2OD1zLkWgxtEhOC5{IN88US=> M{TiNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkixNlA2Lz5{NE[4NVIxPTxxYU6=
K-562 NXjuPHBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfXdoh5PzMEoHlCpC=> MmHVS2k2OD1zLkigxtEhOC5zIN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ6MUKwOUc,OjR4OEGyNFU9N2F-
NCTC-2544 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnOV5dtPzMEoHlCpC=> MnHSS2k2OD12Lk[gxtEhOC5|IN88US=> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ6MUKwOUc,OjR4OEGyNFU9N2F-
A-431 M1\T[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjNc3k4OsLiaNMg NFTOTWhIUTVyPUKuOEDDuSByLkOg{txO M3\6XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkixNlA2Lz5{NE[4NVIxPTxxYU6=
SK-N-SH MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7ZemYxNjZ{NT2yNEDPxE1? NXv6VW5ZPDhiaB?= NH7E[ZNFVVOR M33T[olvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz M2XVb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{m5NFc1Lz5{NEO5PVA4PDxxYU6=
SH-SY5Y NVfnO|NwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXKwMlYzPS1{MDFOwG0> NWrKOoM6PDhiaB?= NEK3d5ZFVVOR NX\x[HFOcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? M2C4R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{m5NFc1Lz5{NEO5PVA4PDxxYU6=
SK-N-SH MnnsRZBweHSxc3nzbUBCe3OjeR?= MmjWOU8yOC9{MDFOwG0> MYq0PEBp NYrwfZN2TE2VTx?= NFn6d3lqdmS3Y3XzJIFxd3C2b4Ppd{Bld3OnIHTldIVv\GWwdHz5 NITSXZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO5PVA4PCd-MkSzPVkxPzR:L3G+
SH-SY5Y NEXmTHlCeG:ydH;zbZNqKEG|c3H5 NFvMSlA2NzFyL{KwJO69VQ>? MVG0PEBp NIm4OW9FVVOR M3XMZolv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN7OUC3OEc,OjR|OUmwO|Q9N2F-
SK-N-SH MX7GeY5kfGmxbjDBd5NigQ>? MnjvOU8yOC9{MDFOwG0> MXm0PEBp NGP2c3pFVVOR NEPqPFJqdmS3Y3XzJGcyKHCqYYPlJINmdGxiY4njcIUh[XK{ZYP0 NYT2ZXNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVkxPzRpPkK0N|k6ODd2PD;hQi=>
SH-SY5Y NH\ad45HfW6ldHnvckBCe3OjeR?= M{PsSlUwOTBxMkCg{txO NWPzZXdVPDhiaB?= NVi4Rm5wTE2VTx?= MoHNbY5lfWOnczDHNUBxcGG|ZTDj[YxtKGO7Y3zlJIFzemW|dB?= MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|OUmwO|QoRjJ2M{m5NFc1RC:jPh?=
SK-N-SH MofXSpVv[3Srb36gRZN{[Xl? MWOxM|UwOTBizszN MmOyOFghcA>? MXHEUXNQ NGDMWFhqdmirYnn0d{BTTVRicHjvd5Bpd3K7bHH0bY9v NFTZd3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO5PVA4PCd-MkSzPVkxPzR:L3G+
SH-SY5Y MXHGeY5kfGmxbjDBd5NigQ>? MUKxM|UwOTBizszN MknBOFghcA>? NXvhdZE2TE2VTx?= MmL0bY5pcWKrdIOgVmVVKHCqb4PwbI9zgWyjdHnvci=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN7OUC3OEc,OjR|OUmwO|Q9N2F-
SK-N-SH NUXofVRMTnWwY4Tpc44hSXO|YYm= NWn4cHZ7PS9zMDFOwG0> MW[0PEBp M1TQNGROW09? NGD6W2ZqdmirYnn0d{BpfW2jbjDORkBk\WyuIH3p[5JifGmxbh?= NYnqOFFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVkxPzRpPkK0N|k6ODd2PD;hQi=>
SH-SY5Y NIjzPHBHfW6ldHnvckBCe3OjeR?= NF3tO|Q2NzFyIN88US=> MkXaOFghcA>? NYnPd3FtTE2VTx?= Moi3bY5pcWKrdIOgbJVu[W5iTlKgZ4VtdCCvaXfyZZRqd25? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN7OUC3OEc,OjR|OUmwO|Q9N2F-
SK-N-SH Mm\BSpVv[3Srb36gRZN{[Xl? NXXzd5ZKPS9zMDFOwG0> MkXTOFghcA>? NUCz[XFtTE2VTx?= NWTEdJZwcW6qaXLpeJMhcHWvYX6gUmIh[2WubDDpcpZie2mxbh?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN7OUC3OEc,OjR|OUmwO|Q9N2F-
SH-SY5Y NWLXV2lHTnWwY4Tpc44hSXO|YYm= NHHEOGs2NzFyIN88US=> NVLxXI9wPDhiaB?= M{PVeWROW09? NUHCOlhicW6qaXLpeJMhcHWvYX6gUmIh[2WubDDpcpZie2mxbh?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN7OUC3OEc,OjR|OUmwO|Q9N2F-
SK-N-SH MmnYSpVv[3Srb36gRZN{[Xl? M33aUVUh|ryP MWGyOE81QC95MjDo M3jIeWROW09? NH\UTWp{fXCycnXzd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEO[Q2K0JIFv\CCPTWCxOEBuWk6D NWP5NoV4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVkxPzRpPkK0N|k6ODd2PD;hQi=>
SH-SY5Y NFnTe2FHfW6ldHnvckBCe3OjeR?= NYPqVoVRPSEQvF2= M2DWWVI1NzR6L{eyJIg> MlPpSG1UVw>? M{fSb5N2eHC{ZYPz[ZMhfGinIHX4dJJme3Orb36gc4YhS1iFUkSgZY5lKE2PUEG0JI1TVkF? MlToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|OUmwO|QoRjJ2M{m5NFc1RC:jPh?=
SK-N-SH MULGeY5kfGmxbjDBd5NigQ>? NIHxXHY2KM7:TR?= M4WydlQ5Nzd{IHi= NFyxbYpFVVOR M4rX[ZN2eHC{ZYPz[ZMh\XiycnXzd4lwdiCxZjD0bIUhS1iFUkSgZY5lKE2PUEG0JJBzd3SnaX6= NW\lTIp[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVkxPzRpPkK0N|k6ODd2PD;hQi=>
SH-SY5Y M4PxRmZ2dmO2aX;uJGF{e2G7 M1LLdVUh|ryP MV:0PE84OiCq M2ewV2ROW09? NYH1bYFTe3WycILld5NmeyCneIDy[ZN{cW:wIH;mJJRp\SCFWFPSOEBidmRiTV3QNVQheHKxdHXpci=> Mo[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|OUmwO|QoRjJ2M{m5NFc1RC:jPh?=
HMEpC M{j2XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17aVlEhdk1vMUCwJO69VQ>? NUDjeGJbPDkEoHlCpC=> M4\LWmROW09? NGPHR5ZqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|OEi0N{c,OjRzM{i4OFM9N2F-
MCF-7 NILYUFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPwbmUyKG6PLUGwNEDPxE1? M3[yVVQ5yqCqwrC= NYToUYFjTE2VTx?= MlTobY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NILCbXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGzPFg1Oyd-MkSxN|g5PDN:L3G+
ZR-75-1 NH22UFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWCxJI5ONTFyMDFOwG0> NH3RemU1QMLiaNMg Mn;ZSG1UVw>? NEPuc2tqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? NEfNfm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGzPFg1Oyd-MkSxN|g5PDN:L3G+
MDA-MB-231 M4TMe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXwbldlOSCwTT2xNFAh|ryP MmCyOFjDqGkEoB?= M1GwWmROW09? NHHtUG5qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? NFqyfms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGzPFg1Oyd-MkSxN|g5PDN:L3G+
MDA-MB-468 M1rCcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVK1Z2c{OSCwTT2xNFAh|ryP MnLXOFjDqGkEoB?= M1rwUWROW09? MmLEbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> MkLBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i4OFMoRjJ2MUO4PFQ{RC:jPh?=
T-47-D M{TBZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TFTFEhdk1vMUCwJO69VQ>? MlzxOFjDqGkEoB?= M2LSRmROW09? MnfwbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NXzoe281RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|g5PDNpPkK0NVM5QDR|PD;hQi=>
U251  M3XBWWZ2dmO2aX;uJGF{e2G7 NYPQfWJUOi92L{lihKnPxOLGs9Mg NXfVToYzPi9zMj:yOEBp M{e3OWROW09? NFnkfHhqdmO{ZXHz[ZMhfGinIFzDN{1KUSCuZY\lcEBqdiCjIITpcYUu\GWyZX7k[Y51KGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NVL0bnM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVk5PTJpPkKzO|k6QDV{PD;hQi=>
U87MG MWrGeY5kfGmxbjDBd5NigQ>? NELq[mwzNzRxOPMAje696oT|wrC= Mn3oOk8yOi9{NDDo MmizSG1UVw>? M37DUYlv[3KnYYPld{B1cGViTFOzMWlKKGyndnXsJIlvKGFidHnt[U1l\XCnbnTlcpQh[W6mIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> Mnm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUm4OVIoRjJ|N{m5PFUzRC:jPh?=
U251  M3y1XmZ2dmO2aX;uJGF{e2G7 NHnT[Wo16oDLzs|iiNPDqA>? MWSyM|YwOTJiaB?= MmDxSG1UVw>? NHXrZot{fXCycnXzd4V{KGKjc3HsJIxmfmWuczDv[kBxcG:|cHjvdplt[XSrb36gc4YhWzZiKGOyN|UwOjN4KTygOGUuSlBzIDjUN|cwPDZrLDDhcoQhSWu2IDjTOFc{MSCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5mesLi MnnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUm4OVIoRjJ|N{m5PFUzRC:jPh?=
U87MG M2XFWGZ2dmO2aX;uJGF{e2G7 NFLsV3E16oDLzs|iiNPDqA>? MkS5Nk83NzF{IHi= NUntW4tLTE2VTx?= NHzY[3R{fXCycnXzd4V{KGKjc3HsJIxmfmWuczDv[kBxcG:|cHjvdplt[XSrb36gc4YhWzZiKGOyN|UwOjN4KTygOGUuSlBzIDjUN|cwPDZrLDDhcoQhSWu2IDjTOFc{MSCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5mesLi MljWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUm4OVIoRjJ|N{m5PFUzRC:jPh?=
H1650  Mo\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7uTnpKSzVyPUOuOeKyOS5{IN88US=> MmDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{N{S3OVgoRjJ|Mke0O|U5RC:jPh?=
HUVECs  NY\qR45QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPleXJ7PzJiaB?= M4\4U2lEPTEEoE2gO{4yKM7:bX;sM2w> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzMUCyO{c,OjJ4MUGwNlc9N2F-
KYN-2  M{Hje2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DVSFczKGh? NYXBTFdPUUN3MNMgQUA5NjFizsztc4wwVA>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzMUCyO{c,OjJ4MUGwNlc9N2F-
HuH-7  MojlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TMbVczKGh? M4fzd2lEPTEEoE2gPU41KM7:bX;sM2w> NHHMeIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xNVAzPyd-MkK2NVExOjd:L3G+
HUVECs  NFrKcnpHfW6ldHnvckBCe3OjeR?= NYnpfGY3OS93L{GwJO69VQ>? Mkm2NUBp MYrzbYdvcW[rY3HueIx6KGmwaHnibZR{KF[HR1\SMVIheGixc4Doc5J6dGG2aX;u Mn;MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUGwNlcoRjJ{NkGxNFI4RC:jPh?=
HAK1-B NVvsenNWTnWwY4Tpc44hSXO|YYm= NXfaZ4p1OS93L{GwJO69VQ>? Mne3NUBp NULrN2J4e3WycILld5NmeyCHR1\SJJBpd3OyaH;yfYxifGmxbh?= M4r1SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkGxNFI4Lz5{Mk[xNVAzPzxxYU6=
UM-22A NX\mbWE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13vNFAuPiEQvF2= M{\2clczKGh? NV;TU5pITE2VTx?= NXHEWYNncW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? M4fPblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
UM-22B NYG3TWx4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzwZZlOOC14IN88US=> MornO|IhcA>? NWmyS5pZTE2VTx?= MkXybY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjNyN{ezOUc,OjJ|MEe3N|U9N2F-
PCI-37A NHXZfppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;INE03KM7:TR?= NU\SO|IzPzJiaB?= NIHTPWRFVVOR M1zrOIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz NX;xR3Z5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNFc4OzVpPkKyN|A4PzN3PD;hQi=>
PCI-37B MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLBdnhDOC14IN88US=> NF;UTWM4OiCq MmjDSG1UVw>? NI\DUI1qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MmDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MEe3N|UoRjJ{M{C3O|M2RC:jPh?=
PCI-15B MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3Kwd|AuPiEQvF2= NIruUIQ4OiCq MojvSG1UVw>? MXzpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> Mn34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MEe3N|UoRjJ{M{C3O|M2RC:jPh?=
SCC-25 M1Xl[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPHW3RvOC14IN88US=> NHTGVVA4OiCq MYDEUXNQ Mlj3bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> M{f4WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
UM-22A M4jHdWZ2dmO2aX;uJGF{e2G7 MWKwMVExKM7:TR?= NF3GTWkzPCCq Ml[0SG1UVw>? MmP6bY5pcWKrdIOgeIhmKGGldHn2ZZRqd25ib3[geIhmKEWJRmKgeJlzd3OrbnWgb4lv[XOnIHHu[EBidHOxIHTlZ5Jm[XOnczD0bIUh\XiycnXzd4lwdiCxZjDwbI9{eGixconsZZRm\CCob4Ltd{Bw\iC2aHWg[I94dnO2cnXhcUB{cWewYXzpcoch\WynbXXueJMtKFOWQWSzJIFv\CCPQWDL M{fyVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
UM-22B NWn0UoRrTnWwY4Tpc44hSXO|YYm= NXr0fm5oOC1zMDFOwG0> M1fRVFI1KGh? NFzCUYlFVVOR NIqy[ndqdmirYnn0d{B1cGViYXP0bZZifGmxbjDv[kB1cGViRVfGVkB1gXKxc3nu[UBscW6jc3WgZY5lKGGuc3:g[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIH\vdo1{KG:oIITo[UBld3ewc4Ty[YFuKHOrZ37hcIlv\yCnbHXt[Y51eyxiU2TBWFMh[W6mIF3BVGs> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjNyN{ezOUc,OjJ|MEe3N|U9N2F-
PCI-15B NVjrS3FiTnWwY4Tpc44hSXO|YYm= NI\3TI0xNTFyIN88US=> M{DuNVI1KGh? M1iwXGROW09? M4rndYlvcGmkaYTzJJRp\SCjY4TpeoF1cW:wIH;mJJRp\SCHR1\SJJR6em:|aX7lJItqdmG|ZTDhcoQh[Wy|bzDk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gdIhwe3Cqb4L5cIF1\WRiZn;ycZMhd2ZidHjlJIRwf26|dILlZY0he2mpbnHsbY5oKGWuZX3lcpR{NCCVVFHUN{BidmRiTVHQTy=> M3zNWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
PCI-37A MkjhSpVv[3Srb36gRZN{[Xl? NX\0c2p5OSEQvF2= MUmyOEBp NIDyfVdFVVOR MonI[I94dnKnZ4XsZZRmeyCYRVfGJJBzd2S3Y4Tpc44> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjNyN{ezOUc,OjJ|MEe3N|U9N2F-
UM-22A MknPSpVv[3Srb36gRZN{[Xl? NUfNbY03OSEQvF2= NHTROo0zPCCq Mle4SG1UVw>? M4XtPYRwf26{ZXf1cIF1\XNiVlXHSkBxem:mdXP0bY9v Mlr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MEe3N|UoRjJ{M{C3O|M2RC:jPh?=
PCI-15B MmDYSpVv[3Srb36gRZN{[Xl? M1fGN|Eh|ryP MWeyOEBp MnXoSG1UVw>? NX;kUm1[\G:5boLl[5Vt[XSnczDWSWdHKHC{b3T1Z5Rqd25? M3XSelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
PCI-15B MYfJcpZie2mxbjDBd5NigQ>? NGTPXFYzPCCq MU\EUXNQ NX65[YtsTUN3ME21OVghdk1? NV\mVJcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNFc4OzVpPkKyN|A4PzN3PD;hQi=>
PCI-37A MljmTY53[XOrb36gRZN{[Xl? NXPnWFFLOjRiaB?= Ml;JSG1UVw>? NHuwRW9GSzVyPUG2PVUhdk1? Mnm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MEe3N|UoRjJ{M{C3O|M2RC:jPh?=
UM-22A M1n4bGlvfmG|aX;uJGF{e2G7 NVyycoRsOjRiaB?= M{LCfWROW09? MX3FR|UxRTBwMzDuUS=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjNyN{ezOUc,OjJ|MEe3N|U9N2F-
SCC-25 M2Hnc2lvfmG|aX;uJGF{e2G7 NYHU[m1[OjRiaB?= MWHEUXNQ MljvSWM2OD1zMDDuUS=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjNyN{ezOUc,OjJ|MEe3N|U9N2F-
UM-22B NWPpeVhVUW64YYPpc44hSXO|YYm= NHfB[JIzPCCq NULM[VRmTE2VTx?= NX[zWW9qTUN3ME2yOFI1KG6P NGniSG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOwO|c{PSd-MkKzNFc4OzV:L3G+
PCI-37B M4O1eGlvfmG|aX;uJGF{e2G7 NYq0elBuOjRiaB?= M1nCZWROW09? NYfkS5VxTUN3ME2xO|I3KG6P M2rRflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C3O|M2Lz5{MkOwO|c{PTxxYU6=
201T NUj1UpZ{TnWwY4Tpc44hSXO|YYm= NYfubnhiOi53IN88US=> M3LaUVQ5KGh? MoDzSG1UVw>? M3XGfolvcGmkaYTzJJBpd3OyaH:tUWFRUyCob3zsc5dqdmdiRVfG MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ3OES3Okc,OjJ{NUi0O|Y9N2F-
273T  NISx[W5HfW6ldHnvckBCe3OjeR?= NX7WVY9[Oi53IN88US=> NXjHVnNrPDhiaB?= NFL6eGxFVVOR NX7tbYVkcW6qaXLpeJMheGixc4Doc{1OSVCNIH\vcIxwf2mwZzDFS2Y> NXnDXGRHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOVg1PzZpPkKyNlU5PDd4PD;hQi=>
A549 M2XUXGZ2dmO2aX;uJGF{e2G7 NUf6XFZ3Oi53IN88US=> M{Hq[VQ5KGh? MVjEUXNQ MoPtbY5pcWKrdIOgdIhwe3Cqbz3NRXBMKG[xbHzve4lv\yCHR1[= NVu0OnMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOVg1PzZpPkKyNlU5PDd4PD;hQi=>
201T  NWXqNos4TnWwY4Tpc44hSXO|YYm= MmnBNU82NzFyIN88US=> MlHpOFghcA>? Mn;SSG1UVw>? M1;0XIJtd2OtczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKEGtdDDpcoR2[2WmIHL5JHZGT0[F NFn0cHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK1PFQ4Pid-MkKyOVg1PzZ:L3G+
H2052 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3UTWM2OD1zLkC3xtExNjB2IN88US=> NGnSPGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm3NFg4PCd-MkG5O|A5PzR:L3G+
H2452 Mo\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfYfVBTUUN3ME2zMlUzyrFzLkGzJO69VQ>? M3vO[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUewPFc1Lz5{MUm3NFg4PDxxYU6=
H28 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPPOlhWUUN3ME2wMlMzyrFyLkC3JO69VQ>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl5MEi3OEc,OjF7N{C4O|Q9N2F-
MSTO-211H M1zCV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\Kb4NKSzVyPUGuOFLDuTBwMEOg{txO M4XqZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUewPFc1Lz5{MUm3NFg4PDxxYU6=
Hth83 Mlj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3m3SlczKGh? Mn;nSG1UVw>? MU\JR|UxRTNwM{CgxtEhOC54NjFOwG0> M3zkUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkKwOFc4Lz5{MUKyNFQ4PzxxYU6=
C643 MoKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDCO|IhcA>? NF;XPYpFVVOR NVW2dnBLUUN3ME2zMlY2KMLzIEGuNlIh|ryP MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ{MES3O{c,OjF{MkC0O|c9N2F-
8505C MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PoUVczKGh? MYXEUXNQ MV7JR|UxRTdwNU[gxtEhOS5zMzFOwG0> NYfnfXNiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyNlA1PzdpPkKxNlIxPDd5PD;hQi=>
Hth74 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3ZTYg2PzJiaB?= MWPEUXNQ MXzJR|UxRThwNU[gxtEhOS5yMTFOwG0> NIWzbJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUKyNFQ4Pyd-MkGyNlA1Pzd:L3G+
SW1736 M4f3d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3izRlczKGh? NVTqblU1TE2VTx?= MkezTWM2OD17LkC1JOKyKDBwNUWg{txO MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ{MES3O{c,OjF{MkC0O|c9N2F-
Hth7 NELwbHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13ve|czKGh? M2G4PWROW09? MkDpTWM2OD17Lk[2JOKyKDBwM{ig{txO NUDlOZJpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyNlA1PzdpPkKxNlIxPDd5PD;hQi=>
Hth104 MkHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV23NkBp M2r1U2ROW09? NWTEVZBWUUN3ME5CtVE3Njl6INMxJG5CKM7:TR?= NHH0cnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUKyNFQ4Pyd-MkGyNlA1Pzd:L3G+
HTB3 NYL1eFNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIj0epAxNTJyIN88US=> MmrGNlTDqGh? NV;TNYZUcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? NFzNWmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFI2Pid-MUmyNlAzPTZ:L3G+
HT1376 NUHoTHVJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGP0T5kxNTJyIN88US=> M3:0c|I1yqCq NWO5O4tDcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MmD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{MkCyOVYoRjF7MkKwNlU3RC:jPh?=
RT4 M33zOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jFNVAuOjBizszN NEH0eYszPMLiaB?= MoHSbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NHHKZoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFI2Pid-MUmyNlAzPTZ:L3G+
J82 M3[3VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\hRlAuOjBizszN MWiyOOKhcA>? MVHpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> NFK5Zok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFI2Pid-MUmyNlAzPTZ:L3G+
CRL1749 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfzeWIxNTJyIN88US=> NUTqfoFVOjUEoHi= NGLoSIpqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? NH33[GU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFI2Pid-MUmyNlAzPTZ:L3G+
T24 NES4NohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIru[40xNTJyIN88US=> NWjsemF{OjUEoHi= M{HD[4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ{MEK1Okc,OTl{MkCyOVY9N2F-
SUP NVXW[VhHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGf2[JkxNTJyIN88US=> Mn:2NlTDqGh? NUjCSpgxcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? NGHoXWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFI2Pid-MUmyNlAzPTZ:L3G+
HTB9 MlPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XjXlAuOjBizszN NULYU2prOjUEoHi= NGnwdoxqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? M3vEV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkKwNlU3Lz5zOUKyNFI2PjxxYU6=
ACC3 M{Tob2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHJNE0yOCEQvF2= MYG3NkBp Mmf5bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NGnHdXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5PFAzPSd-MUi2PVgxOjV:L3G+
ACC2 M2HVXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPNb3l7OC1zMDFOwG0> MoC3O|IhcA>? M33nRYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7OECyOUc,OTh4OUiwNlU9N2F-
ACCM NI[4WY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWSwMVExKM7:TR?= NUXBbGRmPzJiaB?= NXjURVVqcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7OECyOUc,OTh4OUiwNlU9N2F-
ACC3 NWXPT2hCSXCxcITvd4l{cSCDc4PhfS=> NIXsbZcxNTFyIN88US=> M17SWVczKGh? NXH3TIpbcW6mdXPld{BieG:ydH;zbZMh\G:|ZTDk[ZBmdmSnboTsfS=> NF;NW5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5PFAzPSd-MUi2PVgxOjV:L3G+
ACC2 NYTqXWRmSXCxcITvd4l{cSCDc4PhfS=> M1XZe|AuOTBizszN NG[1UmY4OiCq NHi3NoxqdmS3Y3XzJIFxd3C2b4Ppd{Bld3OnIHTldIVv\GWwdHz5 MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7OECyOUc,OTh4OUiwNlU9N2F-
ACCM MWnBdI9xfG:|aYPpJGF{e2G7 M{Kx[VAuOTBizszN MYO3NkBp M3Hwdolv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= NGHaNGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5PFAzPSd-MUi2PVgxOjV:L3G+
EHMES-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYK3NkBp NFLleotFVVOR NGHWe49KSzVyPUGwMlYh|ryP M3HJR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{[0NlQ5Lz5zOEO2OFI1QDxxYU6=
EHMES-10 MnvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWK3NkBp NFjKSVFFVVOR NGL4d4VKSzVyPUCuN{DPxE1? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN4NEK0PEc,OTh|NkSyOFg9N2F-
211H MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXiO|IhcA>? NHjq[o5FVVOR M2jjXGlEPTB;Mj6yJO69VQ>? M2jTS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{[0NlQ5Lz5zOEO2OFI1QDxxYU6=
H28 M4\nWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrmO|IhcA>? NHjWeWxFVVOR M{iyW2lEPTB;MT64JO69VQ>? MlnDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|NkSyOFgoRjF6M{[0NlQ5RC:jPh?=
H2052 MmTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLUWno4PzJiaB?= MX\EUXNQ MnWyTWM2OD16LkCg{txO NXjnb5hKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOlQzPDhpPkG4N|Y1OjR6PD;hQi=>
H2452 NGTLTpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfqeZBuPzJiaB?= Ml;ISG1UVw>? NWDLW3g{UUN3ME21MlUh|ryP NGDUfpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO2OFI1QCd-MUizOlQzPDh:L3G+
CNE-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV2wMlEuOjVwNjFOwG0> NXXmNYFTPDhiaB?= MYfJR|UxRTNwNjFOwG0> NGTNenQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[zNVY1Pid-MUe2N|E3PDZ:L3G+
CNE-2 MorBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnftNE4yNTJ3Lk[g{txO MlSxOFghcA>? MoLnTWM2OD14LkKg{txO MkWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4M{G2OFYoRjF5NkOxOlQ3RC:jPh?=
C666-1 MnvJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTWZVVGOC5zLUK1MlYh|ryP MlW1OFghcA>? MWXJR|UxRTJ|LkSg{txO M3\3eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkOxOlQ3Lz5zN{[zNVY1PjxxYU6=
CNE-1 NH7VXnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjBSJYxNjFvMkWuOkDPxE1? MWC3NkBp Mn3zTWM2OD1{LkOg{txO NUnkdGVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|E3PDZpPkG3OlMyPjR4PD;hQi=>
CNE-2 NWTL[lRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLPOWFNOC5zLUK1MlYh|ryP Ml3TO|IhcA>? NIG0W2NKSzVyPUOuOkDPxE1? M37FS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkOxOlQ3Lz5zN{[zNVY1PjxxYU6=
C666-1 NULLT|ZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF20U4QxNjFvMkWuOkDPxE1? NWn5[G9yPzJiaB?= MWjJR|UxRTRwOE[g{txO NWTQPXNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|E3PDZpPkG3OlMyPjR4PD;hQi=>
CNE-1 NH3qT5JHfW6ldHnvckBCe3OjeR?= NX\HRWkzPiEQvF2= NVHSRoVUOjRiaB?= M3e2U4RmdGG7czDHNE9IOSClZXzsJIN6[2ynIIDyc4dz\XO|aX;u M3:zPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkOxOlQ3Lz5zN{[zNVY1PjxxYU6=
CNE-2 M4GwXWZ2dmO2aX;uJGF{e2G7 Mn\GOkDPxE1? M4HCflI1KGh? NX2wV3k1\GWuYYnzJGcxN0dzIHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44> NHz5fIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[zNVY1Pid-MUe2N|E3PDZ:L3G+
C666-1 MWnGeY5kfGmxbjDBd5NigQ>? NF3mc2o3KM7:TR?= NVXSfZRLOjRiaB?= NILTN|Fl\WyjeYOgS|AwTzFiY3XscEBkgWOuZTDwdo9oemW|c3nvci=> NUXnO4hkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|E3PDZpPkG3OlMyPjR4PD;hQi=>
KDR15 MWLGeY5kfGmxbjDhd5NigQ>? NH62OFBKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhXkWJRjDzeIlufWyjdHXkJIF2fG:yaH;zdIhwenmuYYTpc44hd2ZiVlXHSnIzKGW6cILld5Nm\CCrbjDLSHIyPSClZXzsd{whUUN3MDC9JFAvODF3IN88UU4> M2TONVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{CyO|k4Lz5zNkOwNlc6PzxxYU6=
Sf9 NWq5cod6TnWwY4Tpc44h[XO|YYm= NWTKO3o4UW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDobZN1cWSrbnWteIFo\2WmIGLFWEApPzByLUGwNlAqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZpkhTUyLU1GsJGlEPTBiPTCwMlA6PyEQvF2u NEHU[GI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwPVYyQCd-MkC0NFk3OTh:L3G+
TPC1 NWPaUYk{SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVW3NkBpenN? M1vJOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVGDDNUBk\WyuczDlfJBz\XO|aX7nJHJGXC:SQ2SxJIFnfGW{IEeyJIhzeyCkeTDbN2hefGi7bXnkbY5mKGmwY3;ydI9z[XSrb36gZZN{[XluIFnDOVAhRSByLkGxOkDPxE1w NITJRYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwPVYyQCd-MkC0NFk3OTh:L3G+
HEK293 M3jjZmZ2dmO2aX;uJIF{e2G7 NWKzbWZ[UW6qaXLpeIlwdiCxZjDGS2ZTOS:YRVfGVlIh[2irbXXybYMh[2:wc4TyeYN1KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[nliRVzJV2EtKEWGNUCgQUAxNjF3IN88UU4> NX30T3l1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNFEyPTVpPkG5NVAyOTV3PD;hQi=>
Sf21 NIKxdHZHfW6ldHnvckBie3OjeR?= Mm\3NVUhdWmwcx?= MnrNTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDIbZMufGGpZ3XkJIh2dWGwIFvEVkBmgHC{ZYPz[YQhcW5iaX7z[YN1KFOoMkGgZ4VtdHNicILlbY5kfWKjdHXkJIZweiBzNTDtbY5{KG[xbHzve4VlKGK7IIP1ZpN1emG2ZTDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{MDDtbY5{KGK7IFjUVmYh[XO|YYmsJGlEPTBiPTCwMlE4PSEQvF2u MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh5NEe0NUc,OjZ6N{S3OFE9N2F-
umbilical vein endothelial cells MXvGeY5kfGmxbjDhd5NigQ>? M2nOU2lvcGmkaYTpc44hd2ZiVlXHSk1qdmS3Y3XkJJBzd2yrZnXyZZRqd25ib3[gbJVu[W5idX3ibYxq[2GuII\lbY4h\W6mb4To[Yxq[WxiY3XscJMtKEmFNUCgQUAxNjRizszNMi=> NUnESJZwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3OFMzODJpPkG1O|Q{OjB{PD;hQi=>
BA/F3 MnXySpVv[3Srb36gZZN{[Xl? M3fOWVQ5KGi{cx?= MUDJcohq[mm2aX;uJI9nKEuLRkXCM3JGXCBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDtc5V{\SCEQT;GN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGNmdGxidHn0doUh\2yxLXLhd4VlKGy3bXnu[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwNDFOwG0v NV7iWIJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4O|Q4PDFpPkK2PFc1PzRzPD;hQi=>
BA/F3 NF3PUm9HfW6ldHnvckBie3OjeR?= MlvoOFghcHK| M1HlTmlvcGmkaYTpc44hd2ZiS1TSJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKG2xdYPlJGJCN0Z|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliQ3XscEB1cXS{ZTDncI8u[mG|ZXSgcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE43OyEQvF2u NFv1Z|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki3OFc1OSd-Mk[4O|Q4PDF:L3G+
umbilical vein endothelial cells Mn3ZSpVv[3Srb36gZZN{[Xl? NGexW5pKdmirYnn0bY9vKG:oIHLhd4lkKE[JRj3pcoR2[2WmIIDyc4xq\mW{YYTpc44hd2ZiaIXtZY4hfW2kaXzpZ4FtKH[naX6g[Y5ld3SqZXzpZYwh[2WubIOsJGlEPTBiPTCxMlIh|ryPLh?= MkHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5NEOyNFIoRjF3N{SzNlAzRC:jPh?=
HL60 Ml7iRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NEnsZnY4OiCqcoO= MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwNEmyJO69VS5? M4nwfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm1OVI4Lz5{Nkm5OVUzPzxxYU6=
293 M4\2d2Z2dmO2aX;uJIF{e2G7 MYTJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgWmVITlJ{IITyZY5{cWWwdHz5JJRz[W6|ZnXjeIVlKGmwIEK5N{Bi\GWwb4\pdpV{KHS{YX7z[oVkfGWmIHvp[I5mgSClZXzsd{BjgSCHTFnTRUwhUUN3MDC9JFEvPjZizszNMi=> MnfFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{N{WwO|IoRjF4Mke1NFczRC:jPh?=
293 Ml\ZSpVv[3Srb36gZZN{[Xl? NXP3TY9IUW6qaXLpeIlwdiCxZjDWSWdHWjJiaX6gNlk{KGGmZX7veolzfXNidILhcpNn\WO2ZXSgb4lldmW7IHPlcIx{KGK7IHPlcIwu[mG|ZXSgSWxKW0FiYYPzZZktKEmFNUCgQUAyNjZ4IN88UU4> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjN{MUWzNUc,OTZ|MkG1N|E9N2F-
HEK293 MUjGeY5kfGmxbjDhd5NigQ>? NEeweHVKdmirYnn0bY9vKG:oIG\FS2ZTOiCyaH;zdIhwenmuYYTpc44hcW5iSFXLNlk{KGOnbHzzJIJ6KGOnbHytZoF{\WRiRVzJV2EtKEmFNUCgQUAyNjZ4IN88UU4> NUmx[JdKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0OlA6OzZpPkG2OFYxQTN4PD;hQi=>
HT-29 NYm4bZBpSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1nTUlczKGi{cx?= M{D3V2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuPVI2KM7:TT6= NFLHb|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OVUzPyd-Mk[5PVU2Ojd:L3G+
DU145 MXzBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{HQUlczKGi{cx?= MmPQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT65O|Qh|ryPLh?= NGT3bVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OVUzPyd-Mk[5PVU2Ojd:L3G+
MGHU3 MlvPRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NYXjeldjPzJiaILz NH7yPZRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3HTHU{KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyIE2gNk42KM7:TT6= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODNyOU[3NUc,OzB|MEm2O|E9N2F-
A549 NX3xTFZxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYq3NkBpenN? NWLv[WVjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyIE2gNk42KM7:TT6= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODNyOU[3NUc,OzB|MEm2O|E9N2F-
RT112 NVnOV|lTSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NY\UcG5DPzJiaILz MUXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKWMUGyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7LDDJR|UxKD1iMj61JO69VS5? M2X5OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{C5OlcyLz5|MEOwPVY4OTxxYU6=
A549 M3i0WWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGTH[ok4OiCqcoO= MkThRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{Lk[zJO69VS5? NF\FUJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OVUzPyd-Mk[5PVU2Ojd:L3G+
CHO NV;zem5mTnWwY4Tpc44h[XO|YYm= M2\LVGlvcGmkaYTpc44hd2ZiVlXHSnIhcW6mdXPl[EBifXSxcHjvd5Bpd3K7bHH0bY9vKG:oIHj1cYFvKF[jc3P1cIFzKGWwZH;0bIVtcWGuIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhOiBqVlXHSnIzMSC2cnHud4Zm[3SnZDDpckBEUE9iY3XscJMtKEmFNUCgQUAzNjZ5MzFOwG0v MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR5N{O1Nkc,OTJ2N{ezOVI9N2F-
MCF7 NXH4dHBGSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MoD3O|IhcHK| MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDNwNUO2JO69VS5? M1nMdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm1OVI4Lz5{Nkm5OVUzPzxxYU6=
PANC1 M4XqV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NH\Ed4o4OiCqcoO= Ml3IRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQV7DNUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iND6xNFch|ryPLh?= M2W5T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm1OVI4Lz5{Nkm5OVUzPzxxYU6=
HT-29 MWDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mni5NVAhfU1? MVe3NkBpenN? NF7mZ|dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTBiPTC0MlIh|ryPLh?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN3M{W0Okc,OjF|NUO1OFY9N2F-
EAhy926 M1fFVGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGHMfZAyOCC3TR?= NXnzNXdLPzJiaILz MkLORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCHQXj5PVI3KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTBiPTC1MlEh|ryPLh?= M4\WeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{WzOVQ3Lz5{MUO1N|U1PjxxYU6=
MCF7 NUWxWnF7SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MU[0PEBpenN? MljtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH3lZZN2emWmIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzMT64N{DPxE1w NUnWelloRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PFgyQDBpPkK3Olg5OThyPD;hQi=>
MCF7 NH75bHBEgXSxdH;4bYNqfHliYYPzZZk> MXu0PEBpenN? MlyxR5l1d3SxeHnjbZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJIdzd3e2aDDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNj61NkDPxE1w NWXsWVlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
MCF7 MkPNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MkfrOFghcHK| NFPjOotCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG4MlUh|ryPLh?= NF3ke|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke0NVM2QCd-Mk[3OFE{PTh:L3G+
MCF7 M2\U[GFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGTHZ2c1QCCqcoO= MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF6LkWg{txONg>? M1HnblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEe1OVE6Lz5{NkS3OVUyQTxxYU6=
HT-29 NXKzOXg1SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MXK0PEBpenN? MnGzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDt[YF{fXKnZDDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUiuPVUh|ryPLh?= MnH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OEixPFAoRjJ5Nki4NVgxRC:jPh?=
H460 M3vDc2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3\TZVQ5KGi{cx?= NY\ZTo41SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYxKGOnbHzzJI1m[XO3cnXkJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzO{4yKM7:TT6= NGn5OHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PFE5OCd-Mke2PFgyQDB:L3G+
A673 M{[2[ZFJXFNiYYPzZZk> NX7VSYJyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= NIfTWnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY MkL6dWhVWyCjc4PhfS=> NWj4fJVCeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= NV\3fZJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 M3zyRpFJXFNiYYPzZZk> M4rvRZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= Mo\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD M{XQ[JFJXFNiYYPzZZk> MnKxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ NWPhUppKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH NF3jOWVyUFSVIHHzd4F6 MnW2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO= NH71PVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MWLxTHRUKGG|c3H5 M3;sbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= M4rLb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MkHodWhVWyCjc4PhfS=> M4e1dZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= M4TESlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MXnxTHRUKGG|c3H5 MnHMdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= MonWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH M1vPe5FJXFNiYYPzZZk> NFi2UnlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLWPIJINmdGy| MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 NXXKbGFReUiWUzDhd5NigQ>? M3HlTpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaESxJINmdGy| MoX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 MVTxTHRUKGG|c3H5 M2m2VJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJM> NFrzVYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC M{f6fpFJXFNiYYPzZZk> NGnPWmFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> M{m3eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 M{HWV5FJXFNiYYPzZZk> MoX5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NUfW[HVRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 MYHxTHRUKGG|c3H5 Mn7BdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? M1vvOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-EGFR / EGFR ; 

PubMed: 20629091     


Dose-dependent inhibition of EGFR phosphorylation by vandetanib in the human HNSCC cell lines FaDu, and SCC61. Cells were serum-starved, treated for 90 min with vandetanib at the indicated concentrations, and then stimulated for 15 min with 50 ng/ml EGF. Whole-cell lysates were obtained and subjected to Western immunoblotting to resolve proteins. Antibodies to total (unphosphorylated) receptors and β-actin were used as protein loading controls.

p-ERK / ERK / p-AKT / AKT ; 

PubMed: 19622715     


Logarithmically growing U87 and T98G cells were incubated with varying concentrations of vandetanib for 24 h. The cells were lysed, and equal amounts of proteins were separated by SDS-PAGE and probed with specific antibodies against phospho-ERK, and phospho-Akt. Western blot analysis was performed as described under Materials and Methods. The blots were subsequently stripped and reprobed against total ERK, Akt, or β-actin. 

20629091 19622715
Growth inhibition assay
Cell viability; 

PubMed: 24261856     


(A) MTT assays of HUVEC with α-santalol, vandetanib or sunitinib, respectively. (B) MTT assays of PC-3 cells with α-santalol, vandetanib or sunitinib, respectively.

24261856
体内研究 Vandetanib (2.5 mg/kg, 静脉注射)逆转 63%VEGF诱导的低血压,但是不会显著影响bFGF诱导的低血压。Vandetanib (100 mg/kg) 抑制79%肿瘤诱导的血管形成。Vandetanib (12.5-100 mg/kg, 口服处理) 作用于人类移植瘤,包括 Calu-6, PC-3, MDA-MA-231, SKOV-3, SW620, A549, A431, B16-F10(AP3) 和 Lewis 肺癌,显著抑制肿瘤生长,而对体重几乎没有影响。[1] Vandetanib单独作用于PC3wt移植瘤,施加似是而非的肿瘤生长刺激作用。Vandetanib按25 mg/kg低剂量处理PC3R移植瘤,与对照组相比,明显显著效果,而按50 mg/kg高剂量处理,与对照组相比,则显著抑制肿瘤生长。相反, Vandetanib 50 mg/kg 和Docetaxel 30 mg/kg 按高剂量联用作用于PC3R细胞,却具有显著的负相互作用。[3]Vandetanib 作用于携带肿瘤的小鼠,抑制肿瘤组织中的VEGFR-2 和EGFR磷酸化,显著降低肿瘤血管密度,增强肿瘤细胞凋亡,抑制肿瘤生长,促进生存,降低肝内转移数量,且上调肿瘤组织中的VEGF, TGF-alpha和EGF。Vandetanib处理与严重不良事件,包括ALT异常,骨髓抑制或体重减轻无关。[4]Vandetanib 作用于携带GEO结肠癌移植瘤(对EGFR 信号受抑制敏感)的裸鼠,抑制肿瘤生长,这种作用存在剂量依赖性。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

激酶抑制实验:

Vandetanib 与酶, 10 mM MnCl2, 和 2 μM ATP在聚(Glu, Ala, Tyr) 6:3:1随机共聚物底物包被的96孔板上温育。通过与小鼠IgG抗-磷酸酪氨酸4G10抗体,一种辣根过氧化物酶连接的羊抗鼠免疫球蛋白抗体,和2,2
细胞实验:

[1]

- 合并
  • Cell lines: Calu-6, PC-3, MDA-MA-231, SKOV-3, SW620, A549, A431, B16-F10(AP3) 和 Lewis肺癌细胞
  • Concentrations: 0.1–100 μM
  • Incubation Time: 72 小时
  • Method:

    肿瘤细胞按预先制定密度接种在各自培养基中,确保在实验期间处于对数生长期 (PC-3细胞每孔500个;其他细胞每孔 1000 个)。 实验板在37oC下 含 CO2环境下温育24小时,然后加入 Vandetanib (0.1–100 μM) 或空白对照 (0.1% DMSO,在培养基中)。实验板再预温育72小时,然后使用beta 计数器通过测定 [3 H]胸甘渗透率而测评价细胞增殖。


    (Only for Reference)
动物实验:

[5]

- 合并
  • Animal Models: 携带 PC-3, Calu-6, SKOV-3, 和 MDA-MB-231肿瘤的雌性无胸腺(nu/nu 基因型) Swiss小鼠
  • Dosages: 12.5 mg/kg/day, 25 mg/kg/day, 50 mg/kg/day, 或 100 mg/kg/day
  • Administration: 口服处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.41 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% CMC Na
30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 475.35
化学式

C22H24BrFN4O2

CAS号 443913-73-3
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03291379 Completed Drug: BTG-002814 Carcinoma Hepatocellular|Metastatic Colorectal Cancer Boston Scientific Corporation|Biocompatibles UK Ltd May 17 2017 Early Phase 1
NCT02495103 Terminated Drug: Vandetanib|Drug: Metformin|Drug: Vandetanib/Metformin Renal Cell Carcinoma|Hereditary Leiomyomatosis|Renal Cell Cancer National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 26 2015 Phase 1|Phase 2
NCT02530411 Unknown status Drug: Fulvestrant|Drug: Vandetanib Neoplasms Velindre NHS Trust|Cancer Research UK|AstraZeneca April 2015 Phase 2
NCT02268734 Unknown status -- Metastatic Sporadic Medullary Thyroid Cancer Fondazione IRCCS Istituto Nazionale dei Tumori Milano April 2014 --
NCT01876784 Active not recruiting Drug: Vandetanib (SAR390530)|Drug: Placebo Differentiated Thyroid Cancer Genzyme a Sanofi Company|Sanofi September 17 2013 Phase 3
NCT01661179 Completed Drug: Vandetanib 300mg Unresectable Locally Advanced or Metastatic Medullary Thyroid Carcinoma Genzyme a Sanofi Company|Sanofi November 2012 Phase 1|Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

VEGFR Signaling Pathway Map

VEGFR Inhibitors with Unique Features

相关VEGFR产品

Tags: 购买Vandetanib (ZD6474) | Vandetanib (ZD6474)供应商 | 采购Vandetanib (ZD6474) | Vandetanib (ZD6474)价格 | Vandetanib (ZD6474)生产 | 订购Vandetanib (ZD6474) | Vandetanib (ZD6474)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID