Flavopiridol (L86-8275)

For research use only. Not for use in humans.

目录号：S1230 别名： NSC 649890 HCl, HMR-1275, Alvocidib 中文名称：夫拉平度

Flavopiridol (L86-8275) Chemical Structure

CAS No. 146426-40-6

Flavopiridol (L86-8275, Alvocidib, NSC 649890, HMR-1275) 与ATP竞争性抑制CDKs，包括CDK1、CDK2、CDK4、CDK6和CDK9的IC50范围为20-100 nM。作用于CDK1、2、4、6、9比作用于CDK7更具有选择性。Flavopiridol最初被发现能抑制EGFR和PKA。Flavopiridol可诱导自噬和内质网应激反应。Flavopiridol可阻滞HIV-1的复制。Phase 1/2。

规格

价格

库存

购买数量

10mM (1mL in DMSO)	RMB 810.81	现货
5mg	RMB 647.01	现货
25mg	RMB 1386.75	现货
100mg	RMB 4668.25	现货
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客户使用Selleck生产的Flavopiridol (L86-8275)发表文献85篇：

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Nat Med, 2015, 10.1038/nm.3855

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Genome Res, 2021, 31(6):981-994

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J Cell Biol, 2021, 220(7)e202008146

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Cell Rep, 2021, 36(3):109394

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J Control Release, 2021, 333:246-257

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PLoS Pathog, 2021, 17(4):e1009567

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Cancers (Basel), 2021, 13(14)3418

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Biomolecules, 2021, 11(6)781

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Nat Commun, 2021, 12(1):808

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Pharmaceuticals (Basel), 2021, 14(7)598

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Mol Cell, 2020, 7;S1097-2765(20)30269-0

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J Clin Invest, 2020, 134132

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Nucleic Acids Res, 2020, 24;gkaa268

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EMBO Mol Med, 2020, e11099

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Dev Cell, 2020, 55(6):707-722.e9

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Cell Rep, 2020, 7;31(1):107485

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Signal Transduct Target Ther, 2020, 26;5:17

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Signal Transduct Target Ther, 2020, 26;5(1):17

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BMC Cancer, 2020, 20(1):984

PubMed: 33046037 ( click the link to review the publication )

Int J Parasitol Drugs Drug Resist, 2020, 14:167-182

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PLoS One, 2020, 15(9):e0239315

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Viruses, 2020, 12(10)E1067

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bioRxiv, 2020, 2020/9/20.4.7.30734

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Cell Signal, 2020, 67:109508

PubMed: 31866490 ( click the link to review the publication )

Cell Res, 2019, 29(9):725-738

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Mol Cell, 2019, 73(4):670-683.e12

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Mol Cell, 2019, 74(4):651-663.e8

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EMBO J, 2019, 38(19):e101704

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Dev Cell, 2019, 49(3):425-443

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Proc Natl Acad Sci U S A, 2019, 116(26):12986-12995

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Cancer Res, 2019, 79(19):5088-5101

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Cancer Res, 2019, 79(9):2208-2219

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PLoS Pathog, 2019, 15(3):e1007667

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Cell Chem Biol, 2019, 26(1):121-130

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Lab Invest, 2019, 99(5):612-624

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Sci Rep, 2019, 9(1):17369

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Sci Adv, 2019, 5(6):eaaw3593

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Nucleic Acids Res, 2019, 47(8):3921-3936

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J Cell Sci, 2019, 132(18)jcs233379

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J Exp Med, 2018, 215(12):3194-3212

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J Exp Med, 2018, 215(12):3115-3135

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Elife, 2018, 7e40228

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Oncogene, 2018, 37(28):3763-3777

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Sci Signal, 2018, 11(530)

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Br J Cancer, 2018,

PubMed: 29241222 ( click the link to review the publication )
Cell Oncol (Dordr), 2018, 41(6):663-675

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Oncotarget, 2018, 9(100):37352-37366

PubMed: 30647837 ( click the link to review the publication )

Oncotarget, 2018, 9(34):23505-23518

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Oncotarget, 2018, 9(5): 6174–6187

PubMed: 29464063 ( click the link to review the publication )

Oncotarget, 2018, 9(9):8560-8572

PubMed: 29492217 ( click the link to review the publication )

Gut, 2017, 66(8):1358-1368

PubMed: 27196599 ( click the link to review the publication )

EMBO J, 2017, 36(2):202-212

PubMed: 27852626 ( click the link to review the publication )

Cell Biosci, 2017, 7:43

PubMed: 28852468 ( click the link to review the publication )

PLoS One, 2017, 12(5):e0177871

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Onco Targets Ther, 2017, 10:439-446

PubMed: 28176922 ( click the link to review the publication )

Oncotarget, 2017, 8(68):112313-112329

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Oncotarget, 2017, 8(35):59476-59491

PubMed: 28938651 ( click the link to review the publication )

Cancer Res, 2016, 76(5):1158-69

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Clin Cancer Res, 2016, 22(10):2555-64

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Mol Cell Proteomics, 2016, 15(10):3203-3219

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Aging (Albany NY), 2016, 8(2):366-81

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Cell Cycle, 2016, 15(20):2780-91

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Nat Commun, 2015, 6:8481

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Leukemia, 2015, 10.1038/leu.2015.99

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Antimicrob Agents Chemother, 2015, 59(4):2062-71

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Sci Rep, 2015, 5:10120

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Front Neural Circuits, 2015, 9:63

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Cancer Chemother Pharmacol, 2015, 76(1):105-16

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Leukemia, 2014, 28(3):629-41

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Water Res, 2014, 61:67-76

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ACS Chem Biol, 2014, 10.1021/cb500072z

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Front Cell Neurosci, 2014, 8:39

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PLoS One, 2014, 9(10):e111146

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Cell Cycle, 2013, 12(23):3689-701

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PLoS One, 2013, 8(12):e83467

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产品安全说明书

CDK抑制剂选择性比较

生物活性

产品描述

特性

Flavopiridol是第一个应用于人临床实验的细胞周期蛋白依赖性激酶抑制剂。

靶点

CDK9 ^[8] (Cell-free assay)	CDK1 ^[8] (Cell-free assay)	CDK4 ^[9] (Cell-free assay)	CDK2 ^[8] (Cell-free assay)	CDK6 ^[9] (Cell-free assay)	点击更多
20 nM	30 nM	20-40 nM	40 nM	60 nM	点击更多

体外研究

作为CDK广谱抑制剂，Flavopiridol可以抑制细胞周期进展，使其停在G1期或G2期。0.3 μM Flavopiridol作用于MCF-7或MDA-MB-468细胞，通过抑制CDK4或CDK2激酶活性，而诱导细胞周期停在G1 期。^[4] Flavopiridol 作用于无关激酶，如MAP, PAK, PKC, 和 EGFR，活性低很多，IC50 >14 μM。Flavopiridol 显著抑制HCT116, A2780, PC3, 和 Mia PaCa-2 细胞集落生长，IC50分别为 13 nM, 15 nM, 10 nM, 和 36 nM。^[1] Flavopiridol作用于多种肿瘤细胞系，具有细胞毒性，IC50为作用于LNCAP 的 16 nM 到作用于 K562的130 nM。^[5] Flavopiridol也有效抑制糖原合成激酶-3(GSK-3) 的活性，IC50为 280 nm。^[2]与其他CDKs相比, Flavopiridol抑制CDK7活性时效果稍弱，IC50为875 nM。 Flavopiridol (0.5 μM) 抑制pSer807/811 Rb 和pThr199 NPM,而在pThr821 Rb上观察到轻微变化。Flavopiridol也降低全部RNA聚合酶 II 水平, 及 RNA聚合酶 II在 CTD重复序列在 Ser2 Ser5位点磷酸化。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MCF-7 tumor cell	MWTQdo9tcW[ncnH0bY9vKGG\|c3H5			NWm4NoxSUW6qaXLpeIlwdiCxZjDNR2YuPyC2dX3vdkBk\WyuIIDyc4xq\mW{YYTpc44>	NHfiOGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEi0N\|IyOSd-MUC4OFMzOTF:L3G+
PC3 cell	NWDUXYZJTnWwY4Tpc44h[XO\|YYm=			NWnwSXo3UW6qaXLpeIlwdiCxZjDQR\|Mh[2WubDDjcI9vd2enbnnjJIF{e2G7LDDJR\|UxRTFyIN88US=>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTB4M{[wPUc,OTFyNkO2NFk9N2F-
HCT116 cell	NXy0dFFCTnWwY4Tpc44h[XO\|YYm=			M4XlZWlvcGmkaYTpc44hd2ZiSFPUNVE3KGOnbHygZ4xwdm:pZX7pZ{Bie3OjeTygTWM2OD1zMzFOwG0>	NGnpWVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUC2N\|YxQSd-MUGwOlM3ODl:L3G+
A2780 cell	MVnGeY5kfGmxbjDhd5NigQ>?			M4PDSWlvcGmkaYTpc44hd2ZiQUK3PFAh[2WubDDjcI9vd2enbnnjJIF{e2G778{MJGlEPTB;MUWg{txO	MlfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFyNkO2NFkoRjFzME[zOlA6RC:jPh?=
Mia PaCa-2 cell	MYjGeY5kfGmxbjDhd5NigQ>?			M4\6R2lvcGmkaYTpc44hd2ZiTXnhJHBiS2FvMjDj[YxtKGOub37v[4VvcWNiYYPzZZktKEmFNUC9N\|Yh\|ryP	M4f1PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzME[zOlA6Lz5zMUC2N\|YxQTxxYU6=
LNCaP human prostate carcinoma cell	M2LBenBzd2yrZnXyZZRqd25iYYPzZZk>			MV7Jcohq[mm2aX;uJI9nKEyQQ3HQJIh2dWGwIIDyc5N1[XSnIHPhdoNqdm:vYTDj[YxtKHC{b3zp[oVz[XSrb36=	NF3JO5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkG5NFMyOyd-MUKxPVA{OTN:L3G+
HCT116/VP35 human colon carcinoma cell	MXLQdo9tcW[ncnH0bY9vKGG\|c3H5			NV3tPY56UW6qaXLpeIlwdiCxZjDIR3QyOTZxVmCzOUBpfW2jbjDjc4xwdiClYYLjbY5wdWFiY3XscEBxem:uaX\ldoF1cW:wLDDJR\|UxRTF5IH7N	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
HCT116 human colon carcinoma cell	MX\Qdo9tcW[ncnH0bY9vKGG\|c3H5			M2ns[mlvcGmkaYTpc44hd2ZiSFPUNVE3KGi3bXHuJINwdG:wIHPhdoNqdm:vYTDj[YxtKHC{b3zp[oVz[XSrb36sJGlEPTB;MUigcm0>	M2XUcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
HCT116/VM46 human colon carcinoma cell	MUDQdo9tcW[ncnH0bY9vKGG\|c3H5			NEjNTIlKdmirYnn0bY9vKG:oIFjDWFEyPi:YTUS2JIh2dWGwIHPvcI9vKGOjcnPpco9u[SClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVA:OjFibl2=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
A2780/DDP-R human ovarian carcinoma cell	MYHQdo9tcW[ncnH0bY9vKGG\|c3H5			NFi2V2xKdmirYnn0bY9vKG:oIFGyO\|gxN0SGUD3SJIh2dWGwIH;2ZZJq[W5iY3HyZ4lvd22jIHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD1\|ODDuUS=>	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
ABAE human fibroblast cell	M{C4e3Bzd2yrZnXyZZRqd25iYYPzZZk>			MkTsTY5pcWKrdHnvckBw\iCDQlHFJIh2dWGwIH\pZpJw[myjc4SgZ4VtdCCycn;sbYZmemG2aX;uMEBKSzVyPUS1JI5O	NHXDeIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkG5NFMyOyd-MUKxPVA{OTN:L3G+
HL60 human leukemia cell	MYjQdo9tcW[ncnH0bY9vKGG\|c3H5			M2rP[WlvcGmkaYTpc44hd2ZiSFy2NEBpfW2jbjDs[ZVs\W2rYTDj[YxtKHC{b3zp[oVz[XSrb36sJGlEPTB;NE[gcm0>	NXzmTZU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxPVA{OTNpPkGyNVkxOzF\|PD;hQi=>
Hs 27 human fibroblast cell	MlLRVJJwdGmoZYLheIlwdiCjc4PhfS=>			NX2xZVBUUW6qaXLpeIlwdiCxZjDId{AzPyCqdX3hckBncWK{b3LsZZN1KGOnbHygdJJwdGmoZYLheIlwdixiSVO1NF02OSCwTR?=	NH7EOYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkG5NFMyOyd-MUKxPVA{OTN:L3G+
CCRF-CEM human leukemia cell	NXLTcoFwWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NV\jXlFqUW6qaXLpeIlwdiCxZjDDR3JHNUOHTTDoeY1idiCuZYXr[Y1q[SClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVA:PTJibl2=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
OVCAR-3 human ovarian carcinoma cell	NYrPPWFwWHKxbHnm[ZJifGmxbjDhd5NigQ>?			M3PHUmlvcGmkaYTpc44hd2ZiT2\DRXIuOyCqdX3hckBwfmG{aXHuJINiemOrbn;tZUBk\WyuIIDyc4xq\mW{YYTpc44tKEmFNUC9OVQhdk1?	NGfzWlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkG5NFMyOyd-MUKxPVA{OTN:L3G+
A2780/DDP-S human ovarian carcinoma cell	M4SxZXBzd2yrZnXyZZRqd25iYYPzZZk>			MmnUTY5pcWKrdHnvckBw\iCDMke4NE9FTFBvUzDoeY1idiCxdnHybYFvKGOjcnPpco9u[SClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVA:PTZibl2=	M13JUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780/TAX-S human ovarian carcinoma cell	MVzQdo9tcW[ncnH0bY9vKGG\|c3H5			M4rsUWlvcGmkaYTpc44hd2ZiQUK3PFAwXEG[LWOgbJVu[W5ib4\hdolidiClYYLjbY5wdWFiY3XscEBxem:uaX\ldoF1cW:wLDDJR\|UxRTZ3IH7N	NWPDSHBQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxPVA{OTNpPkGyNVkxOzF\|PD;hQi=>
LS174T human colon carcinoma cell	NFnaNG1Rem:uaX\ldoF1cW:wIHHzd4F6			MnzITY5pcWKrdHnvckBw\iCOU{G3OHQhcHWvYX6gZ49td25iY3HyZ4lvd22jIHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD14NTDuUS=>	NW[wfJd6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxPVA{OTNpPkGyNVkxOzF\|PD;hQi=>
MCF-7 human breast carcinoma cell	Ml\4VJJwdGmoZYLheIlwdiCjc4PhfS=>			MmDTTY5pcWKrdHnvckBw\iCPQ1[tO{BpfW2jbjDidoVie3RiY3HyZ4lvd22jIHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD14NjDuUS=>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
PC3 human prostate carcinoma cell	Mne2VJJwdGmoZYLheIlwdiCjc4PhfS=>			MVjJcohq[mm2aX;uJI9nKFCFMzDoeY1idiCycn;zeIF1\SClYYLjbY5wdWFiY3XscEBxem:uaX\ldoF1cW:wLDDJR\|UxRTZ4IH7N	NHrad3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkG5NFMyOyd-MUKxPVA{OTN:L3G+
MLF mouse lung fibroblast cell	MnnGVJJwdGmoZYLheIlwdiCjc4PhfS=>			NVvsN\|RQUW6qaXLpeIlwdiCxZjDNUGYhdW:3c3WgcJVv\yCoaXLyc4Jt[XO2IHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD15MjDuUS=>	NVnlc2NORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxPVA{OTNpPkGyNVkxOzF\|PD;hQi=>
LX-1 human lung carcinoma	MmPoVJJwdGmoZYLheIlwdiCjc4PhfS=>			Ml3XTY5pcWKrdHnvckBw\iCOWD2xJIh2dWGwIHz1coch[2G{Y3nuc41iKHC{b3zp[oVz[XSrb36sJGlEPTB;N{Wgcm0>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
A431 human squamous cell	M{LPSXBzd2yrZnXyZZRqd25iYYPzZZk>			MkfMTY5pcWKrdHnvckBw\iCDNEOxJIh2dWGwIIPxeYFud3W\|IHPlcIwh[2G{Y3nuc41iKGOnbHygdJJwdGmoZYLheIlwdixiSVO1NF04PSCwTR?=	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
SKBR-3 human breast carcinoma cell	MlfPVJJwdGmoZYLheIlwdiCjc4PhfS=>			MXfJcohq[mm2aX;uJI9nKFONQmKtN{BpfW2jbjDidoVie3RiY3HyZ4lvd22jIHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD15NzDuUS=>	M3vSUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780/TAX-R human ovarian carcinoma cell	M1\sXHBzd2yrZnXyZZRqd25iYYPzZZk>			MXLJcohq[mm2aX;uJI9nKEF{N{iwM3RCYC2UIHj1cYFvKG:4YYLpZY4h[2G{Y3nuc41iKGOnbHygdJJwdGmoZYLheIlwdixiSVO1NF04QCCwTR?=	M1r4UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
M109 mouse lung carcinoma cell	M2GySnBzd2yrZnXyZZRqd25iYYPzZZk>			MYXJcohq[mm2aX;uJI9nKE1zMEmgcY92e2VibIXu[{Bk[XKlaX7vcYEh[2WubDDwdo9tcW[ncnH0bY9vNCCLQ{WwQVgxKG6P	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
CACO-2 human colon carcinoma cell	NHv4fJZRem:uaX\ldoF1cW:wIHHzd4F6			NGnoUItKdmirYnn0bY9vKG:oIFPBR28uOiCqdX3hckBkd2yxbjDjZZJkcW6xbXGgZ4VtdCCycn;sbYZmemG2aX;uMEBKSzVyPUi2JI5O	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
A549 human lung carcinoma cell	NH;yTVhRem:uaX\ldoF1cW:wIHHzd4F6			MUTJcohq[mm2aX;uJI9nKEF3NEmgbJVu[W5ibIXu[{Bk[XKlaX7vcYEh[2WubDDwdo9tcW[ncnH0bY9vNCCLQ{WwQVk3KG6P	M{\wUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
MIP human colon carcinoma cell	MlzhSpVv[3Srb36gZZN{[Xl?			MXPJcohq[mm2aX;uJI9nKE2LUDDoeY1idiClb3zvckBk[XKlaX7vcYEh[2WubDDsbY5mNCCLQ{WwQVAvOTJizszN	NXjr[nQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxPVA{OTNpPkGyNVkxOzF\|PD;hQi=>
K562 human leukemia cell	MYHQdo9tcW[ncnH0bY9vKGG\|c3H5			Mlj5TY5pcWKrdHnvckBw\iCNNU[yJIh2dWGwIHzleYtmdWmjIHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD1yLkGzJO69VQ>?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
human A2780 cell line	NF;vPHhRem:uaX\ldoF1cW:wIHHzd4F6		NVfnZodQPzJiaB?=	M3Lre2FvfGmycn;sbYZmemG2aY\lJIVn\mWldDDh[4FqdnO2IHj1cYFvKEF{N{iwJINmdGxibHnu[UB4[XNiZHX0[ZJucW6nZDDpckBiKHeqb3zlJINmdGxiN{KgbJIh[3m2b4TvfIlkcXS7IHHzd4F6NCCLQ{WwQVcyKG6P	NVXxO3VQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWwNlc5PjNpPkG1NFI4QDZ\|PD;hQi=>
human ovarian (A2780) cancer cell	NVzxd40{S3m2b4TvfIlkyqCjc4PhfS=>			NVzXTpMyS3m2b4TvfIlkKGWoZnXjeEBwdiCqdX3hckBwfmG{aXHuJEhCOjd6MDmgZ4Fv[2W{IHPlcIwhdGmwZTygTWM2OD15MTDuUS=>	NXHtUm1pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxNlU6PzFpPkG1NVI2QTdzPD;hQi=>
ID8 cells	NXrXRoE{WHKxbHnm[ZJifGmxbjDhd5NigQ>?			Ml21RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDJSFgh[2WubIOsJGlEPTB;NzDuUS=>	NX\XOVNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlM5OjFpPkG3NVI{QDJzPD;hQi=>
MCF7 cells	NYrRZ4d7WHKxbHnm[ZJifGmxbjDhd5NigQ>?			M1zUN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUWNHPyClZXzsd{whUUN3ME2yOkBvVQ>?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{M{iyNUc,OTdzMkO4NlE9N2F-
Sf9 cells	Moj3SpVv[3Srb36gZZN{[Xl?			M4rOcGlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgZ5lkdGmwIFGvR2RMOiCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzMEBKSzVyPUGyJI5O	MlHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MESzOlYoRjF5OUC0N\|Y3RC:jPh?=
human A2780 cells	MlHTSpVv[3Srb36gZZN{[Xl?			MVvJcohq[mm2aX;uJI9nKGOmaz3t[YRq[XSnZDDOVG0heGixc4Doc5J6dGG2aX;uJIF1KHSqckG5PUBqdiCqdX3hckBCOjd6MDDj[Yxtew>?	M2joRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NE[5PFA6Lz5zOES2PVgxQTxxYU6=
human A2780 cells	MU\GeY5kfGmxbjDhd5NigQ>?		NHSzXI0zPCCq	M17WR2lvcGmkaYTpc44hd2ZiY3TrMY1m\GmjdHXkJHJjKHCqb4PwbI9zgWyjdHnvckBifCC2aIK4NlEhcW5iaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOjRiaILz	M3Gx[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NE[5PFA6Lz5zOES2PVgxQTxxYU6=
human NCI60 cells	MYjQdo9tcW[ncnH0bY9vKGG\|c3H5		NXTlfGVlPzJiaB?=	NYDtdYhUSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2k3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF04PC55IH7N	M4DBfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwO\|A{Lz5{MUC4NFcxOzxxYU6=
human NCI60 cells	MnvjVJJwdGmoZYLheIlwdiCjc4PhfS=>		MmPXO\|IhcA>?	NIXZc5hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTVYxKGOnbHzzJIF{e2W\|c3XkJIF{KGyndHjhcEBm\m[nY4SgZYZ1\XJiN{KgbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEyFNUC9NE46ODRizszN	NFTsfHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFcxOyd-MkGwPFA4ODN:L3G+
human A2780 cells	M4LkNmN6fG:2b4jpZ:Kh[XO\|YYm=		MnTHNlQhcA>?	MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBIUTVyPUKzJI5O	M33IdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CxO\|Y4Lz5{M{OwNVc3PzxxYU6=
human MRC5 cells	MlTTR5l1d3SxeHnjxsBie3OjeR?=		MkDoO\|IhcA>?	MoDwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXJEPSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBIUTVyPUK4JI5O	NV\2SVlqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFE4PjdpPkKzN\|AyPzZ5PD;hQi=>
human A2780 cells	M1qye2N6fG:2b4jpZ:Kh[XO\|YYm=		NGPYcWc4OiCq	M3KxXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEeLNUC9Nlkhdk1?	MoHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|MEG3OlcoRjJ\|M{CxO\|Y4RC:jPh?=
human A2780 cells	NGXBTZpEgXSxdH;4bYPDqGG\|c3H5		M1vlNFQ5KGh?	MoDwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhT0l3ME2zNUBvVQ>?	M1TxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CxO\|Y4Lz5{M{OwNVc3PzxxYU6=
human MRC5 cells	M4jyNmN6fG:2b4jpZ:Kh[XO\|YYm=		M2nXelQ5KGh?	NFK2[YxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEeLNUC9N\|khdk1?	M2\vflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CxO\|Y4Lz5{M{OwNVc3PzxxYU6=
human MRC5 cells	MU\DfZRwfG:6aXRCpIF{e2G7		MWqyOEBp	NFPWWI9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEeLNUC9OFkhdk1?	NXj4cXFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFE4PjdpPkKzN\|AyPzZ5PD;hQi=>
human HMEC1 cells	M{W0bmN6fG:2b4jpZ:Kh[XO\|YYm=		MXmyOEBp	NYTke5hES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUE2HQ{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF03OSCwTR?=	Mn\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|MEG3OlcoRjJ\|M{CxO\|Y4RC:jPh?=
human HMEC1 cells	MVPDfZRwfG:6aXRCpIF{e2G7		NH7CT2Q1QCCq	NEDpeVZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVUWFMTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVYzKG6P	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNyMUe2O{c,OjN\|MEG3Olc9N2F-
human HMEC1 cells	NH;WS4NEgXSxdH;4bYPDqGG\|c3H5		M{nu[FczKGh?	NFzXeGhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVUWFMTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVY3KG6P	M3jicFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CxO\|Y4Lz5{M{OwNVc3PzxxYU6=
A2780	NW\2fJgyTnWwY4Tpc44h[XO\|YYm=	M2nqXlM{OCCwTR?=	MkHTNlQhcHJ?	NFTsc2hR\XKlZX70ZYdmKEF{N{iwJINmdGy\|IHnuJJNjNUdzIHHmeIVzKDJ2IHjyJJIh[XRiM{OwJI5OKCiLQ{WwLUwhS2WubDDjfYNt\SB;IECuNFAyKM7:TT6=	M4XYXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780	NFv3OplCeG:ydH;zbZMh[XO\|YYm=	MlHyN\|MxKG6P	M2fpZlI1KGi{	M3H6eWFxd3C2b4TpZ{BCOjd6MDDj[YxteyCjZoTldkAzPCCqcjDyJIF1KDN\|MDDuUUApUUN3MDmsJGNmdGxiY4njcIUhRSByLkCwNUDPxE1w	M3nYR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780	M{n6emZ2dmO2aX;uJIF{e2G7	MonlNlk5OCCwTR?=	NWDWRpZmOjRiaIK=	M3rEPHBmemOnboTh[4UhSTJ5OECgZ4VtdHNiaX6gd5VjNUdzIHHmeIVzKDJ2IHjyJJIh[XRiMkm4NEBvVSBqSVO5NEktKEOnbHygZ5lkdGViPTCwMlAxOiEQvF2u	Ml3yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzOUCzNVMoRjF{MUmwN\|E{RC:jPh?=
A2780	MYnBdI9xfG:\|aYOgZZN{[Xl?	NYS2WY82Ojl6MDDuUS=>	MV[yOEBpeg>?	M2PMO2Fxd3C2b4TpZ{BCOjd6MDDj[YxteyCjZoTldkAzPCCqcjDyJIF1KDJ7OECgcm0hMEmFOUCpMEBE\WyuIHP5Z4xmKD1iMD6wNFkh\|ryPLh?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF7MEOxN{c,OTJzOUCzNVM9N2F-
A2780	MX;GeY5kfGmxbjDhd5NigQ>?	NV3SUmJyOjl6MDDuUS=>	NYK2UFR1OjRiaIK=	MVPQ[ZJk\W62YXflJGEzPzhyIHPlcIx{KGmwIGOtdIhie2ViYX\0[ZIhOjRiaIKgdkBifCB{OUiwJI5OKCiLQ{mwLUwhS2WubDDjfYNt\SB;IECuNFEzKM7:TT6=	Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzOUCzNVMoRjF{MUmwN\|E{RC:jPh?=
A2780	M{\kPWZ2dmO2aX;uJIF{e2G7	NGXWWXQ{OzBibl2=	NWLnboprOjRiaIK=	Mn:xVIVz[2WwdHHn[UBCOjd6MDDj[YxteyCrbjDTMZBp[XOnIHHmeIVzKDJ2IHjyJJIh[XRiM{OwJI5OKCiLQ{WwLUwhS2WubDDjfYNt\SB;IECuNFE3KM7:TT6=	MnX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzOUCzNVMoRjF{MUmwN\|E{RC:jPh?=
A2780	M4jwPGZ2dmO2aX;uJIF{e2G7	M4rEXlM{OCCwTR?=	MoXUNlQhcHJ?	MUTQ[ZJk\W62YXflJGEzPzhyIHPlcIx{KGmwIFeyM20h[W[2ZYKgNlQhcHJicjDheEA{OzBibl2gLGlEPTBrLDDD[YxtKGO7Y3zlJF0hOC5yMkOg{txONg>?	M3vGeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780	M17EZmZ2dmO2aX;uJIF{e2G7	MYeyPVgxKG6P	MXyyOEBpeg>?	M2fHd3BmemOnboTh[4UhSTJ5OECgZ4VtdHNiaX6gS\|IwVSCjZoTldkAzPCCqcjDyJIF1KDJ7OECgcm0hMEmFOUCpMEBE\WyuIHP5Z4xmKD1iMD6wNlUh\|ryPLh?=	M1fCe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUmwN\|E{Lz5zMkG5NFMyOzxxYU6=
A2780	NVnrOZlITnWwY4Tpc44h[XO\|YYm=	NHu0SWo{OzBibl2=	MV[yOEBpeg>?	NWPUTIdPWGW{Y3XueIFo\SCDMke4NEBk\WyuczDpckBIOSCjZoTldkAzPCCqcjDyJIF1KDN\|MDDuUUApUUN3MDmsJGNmdGxiY4njcIUhRSByLkC2JO69VS5?	Mn[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzOUCzNVMoRjF{MUmwN\|E{RC:jPh?=
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A2780	M1y1W2Z2dmO2aX;uJIF{e2G7		MlKyNlQhcHK\|	MoTRTY5pcWKrdHnvckBw\iClZHutcYVlcWG2ZXSgVoIheGixc4Doc5J6dGG2aX;uJIF1KHOnckiwO{85OTFiaX6gbJVu[W5iQUK3PFAh[2WubIOgZYZ1\XJiMkSgbJJ{	M3nGb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NE[5PFA6Lz5zOES2PVgxQTxxYU6=
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A2780	NX7xUIVFTnWwY4Tpc44h[XO\|YYm=		MmLNNlQhcHK\|	NI\SPGVKdmirYnn0bY9vKG:oIHPkb\|cudWWmaXH0[YQhWk6DIIDvcFIhS1SGIIDoc5NxcG:{eXzheIlwdiCjdDDz[ZI2KGmwIHj1cYFvKEF{N{iwJINmdGy\|IHHmeIVzKDJ2IHjydy=>	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR4OUiwPUc,OTh2Nkm4NFk9N2F-
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A2780	M{mxOWNmdGxiY4njcIUh[XO\|YYm=		NXvJUlJEOjRiaILz	MoXrR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gRVI4QDBiY3XscJMh[XO\|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEd{L12gdIhie2ViYYSgcIV{eyC2aHHuJGdKPTBiYX\0[ZIhOjRiaILzJIJ6KG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=>	M4HRelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CxO\|Y4Lz5{M{OwNVc3PzxxYU6=
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SEM	M{Tpd2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		NUG4OGlUPzJiaILz	NYDnd4xSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTSW0h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcHRqfGW{LVfsc{Bie3OjeTygSWM2OCB;IECuNlA1OyEQvF2u	MlrlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MEe5O\|UoRjJ7NEC3PVc2RC:jPh?=
UOCB1	NXS4WnNDSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NEj2b5c4OiCqcoO=	MknSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXT1PCNUBk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XsWIl1\XJvR3zvJIF{e2G7LDDFR\|UxKD1iMD6yNFg1KM7:TT6=	NU\rc3dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NFc6PzVpPkK5OFA4QTd3PD;hQi=>
KOPN8	NXv1U2dOSXCxcITvd4l{KGG\|c3H5	M17zbFAvPSC3TR?=	M1vzWVMhfG9iMkSgbJJ{	MVfJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEuRUF64JINmdGy\|IHHzd4V{e2WmIHHzJJVxemWpdXzheIlwdiCxZjDjcIVifmWmIGDBVnAhdGW4ZXygZZQhOC53IIXNJIFnfGW{IEOgeI8hOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NE\sN2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSwO\|k4PSd-Mkm0NFc6PzV:L3G+
KOPN8	M2jiVGFxd3C2b4Ppd{Bie3OjeR?=	NWHKVnZJOC53IIXN	Mk\ONUBpeg>?	NYexT29xUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDLU3BPQCClZXzsd{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4Yh[2ynYY\l[EBRSVKSIHzleoVtKGG2IECuOUB2VSCycnWteJJm[XSnZDD3bZRpKE6DQzDmc5IhOSCqcjDhcoQhdWWjc4Xy[YQh[W[2ZYKgN{B1dyB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTRyN{m3OUc,Ojl2MEe5O\|U9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cleaved caspase-8 / Cleaved caspase-9 / Cleaved caspase-3; PubMed: 31193061 Heliyon (C) Caspase activation was determined by western blotting. Flavopiridol treatment (300 nM) induced the expressions of cleaved caspase-8, -9 and -3 in a time-dependent manner. p-RNAPII / p-eIF4E / Mnk1 ; PubMed: 24572052 Reprod Sci After 24 h drug exposure, CDKI-73 or flavopiridol also abolished p-eIF4ES209 at 0.25 μM, indicating cellular inhibition of Mnk kinase activity. The same treatment with CDKI-73 or flavopiridol caused a loss in Mnk1 protein expression. CGP57380-treated cells abrogated p-RNAPIIS2 as well as p-eIF4E(S209) with a minimal effect on Mnk1 protein level. p-ERK / ERK / p-p38 / p-4EBP1 / 4EBP1 / p-S6 ; PubMed: 24572052 Reprod Sci 0.25 μM CDKI-73 or flavopiridol caused little changes in the phosphorylation of Erk and p38 MAPK; however, inhibited the 4E-BP1 phosphorylation (p-4E-BP1Thr70) by 24 h. CGP57380 had a minimal effect on these proteins. CDK2 / CDK4 / Cyclin A / p21 / p27 / Rb; PubMed: 24572052 Reprod Sci Effect of flavopiridol on cell cycle-related protein expression in uterine leiomyoma cells. Twenty-four hours after treatment, cell extracts were prepared and subjected to immunoblotting analysis. β-Actin was used as an internal loading control.	31193061 24572052
Growth inhibition assay	Cell viability; PubMed: 31193061 Heliyon The antiproliferative effect of flavopiridol on CCA cell lines was determined using an MTT assay. KKU-055, KKU-100, KKU-213 and KKU-214 cells were treated with 50, 100, 200 or 300 nM of flavopiridol at 24, 48 or 72 h. The percentage of cell number in vehicle control was taken as 100%. Data are mean ± SD of three independent experiments. *P < 0.05 in all CCA cell lines, significantly different for each time point compared with vehicle control.	31193061

体内研究

Flavopiridol按7.5 mg/kg剂量处理P388 小鼠白血病，持续7天，具有轻微抗癌活性，导致%T/C值为110,且有效作用于皮下抑制人A2780卵巢癌的裸鼠，细胞对数杀灭(LCK)为1.5 。^[5] Flavopiridol按 1-2.5 mg/kg 剂量处理小鼠，持续10天，通过抑制滑膜增生和关节损伤，而显著抑制胶原性关节炎，这种作用存在剂量依赖性，然而抗II型胶原(CII) 抗体的血清浓度，和II型胶原对应的增殖维持不变。^[6]

激酶实验： ^{[1] [2] [3]}	- 合并 CDK 激酶实验: CDK1/cyclin B1 激酶实验中, 激酶反应包含100 ng表达 GST-CDK1/cyclin B1(人)复合体的杆状病毒, 1 μg 组蛋白 HI, 0.2 μCi [γ-³³P]ATP, 25 μM ATP溶于 50 μL 激酶buffer (50 mM Tris, pH 8.0, 10 mM MgCl₂, 1 mM EGTA, 0.5 mM DTT)。CDK2/cyclin E 激酶实验中, 激酶反应包含5 ng表达 GST-CDK2/cyclin E (人)复合体的杆状病毒, 0.5 μg GST-RB 融合蛋白(成视网膜细胞瘤蛋白776-928 氨基酸), 0.2 μCi [γ-³³P]ATP, 25 μM ATP溶于 50 μL 激酶buffer (50 mM Hepes, pH 8.0, 10 mM MgCl₂, 1 mM EGTA, 2 mM DTT)。CDK4/cyclin D1激酶实验中, 激酶反应含150 ng 表达GST-CDK4/cyclin D1 (人)的杆状病毒, 280 ng Stag-cyclin D1, 0.5 μg GST-RB 融合蛋白(成视网膜细胞瘤蛋白776-928 氨基酸), 0.2 μCi [γ-³³P]ATP, 25 μM ATP 溶于50 μL激酶 buffer (50 mM Hepes, pH 8.0, 10 mM MgCl₂, 1 mM EGTA, 2 mM DTT)。30^oC下， CDK1 和CDK2反应温育45分钟， CDK4反应温育1小时，加入终浓度为15%的冰冻三氯乙酸(TCA) 终止反应。使用Filtermate通用收集器，收集TCA沉淀物转移到GF/C 非过滤板上，使用TopCount 96孔液体闪烁计数器测量过滤数。Flavopiridol溶于 10 mM二甲基甲酰胺(DMF) 中，测定六种浓度，每种重复测三次。实验中 DMF终浓度为 2%。通过回归曲线分析获得IC50值，变异系数为 16%。测定Flavopiridol作用于 CDK6的活性, 进行过滤-结合实验。反应混合物按如下结合： 2 μL CDK6 (0.7 mg/μL), 5 μL 组蛋白 H1 (6 mg/mL), 14 μL 激酶 buffer (60 mM β-甘油磷酸, 30 mM p-磷酸硝基苯酯, 25 mM MOPS (pH 7.0), 5 mM EGTA, 15 mM MgCl₂, 1 mM DTT, 0.1 mM 钒酸钠), 在50% DMSO中稀释的 3 μL 浓度不断增长的Flavopiridol ，及6 μL ³³P-ATP (1 mCi/mL)，非放射性 ATP，浓度为90 μM，(终浓度为15 μM)。加入³³P-ATP开始反应。反应在30^oC下温育20分钟。25 μL等分的上清液点样到 Whatman P81 磷酸纤维素膜上。使用1% 磷酸溶液冲洗过滤器5次。在1 mL闪烁液存在时测定湿过滤器。使用 50 nM 重组Cdk9/cyclin T在 50 mM HEPES pH 7.5, 10 mM MgCl₂, 1 mM DTT, 3 μM Na₃VO₄, 150 μM RNA 聚合酶CDT 肽和80 μM ATP中测定Cdk9活性。在相同buffer中使用37 nM纯化激酶，在200 μM ATP 存在时，10 μM 髓鞘蛋白（MBP）作为底物，进行Cdk7检测实验。使用强阴离子交换剂-为基础的检测实验或闪烁接近实验测定Flavopiridol 作用于CDK9和CDK7的效果。通过剂量反应曲线计算IC50值。
细胞实验： ^[5]	- 合并 Cell lines: MCF-7, LNCAP, PC3, HCT116, CACO-2, A549, HL60, K562, 等等 Concentrations: 溶于DMSO,终浓度为 ~10 μM Incubation Time: 72小时 Method: 使用不同浓度 Flavopiridol 处理细胞72小时，加入四唑染料，MTS和吩嗪硫酸甲酯。3 小时后,在492 nm处测定吸光值,与存活细胞数成比例。结果表示为IC50值。为了分析细胞周期，细胞在仲甲醛和乙醇中混合，冲洗，然后再悬浮在TdT 酶和 FITC-dUTP染色液中,再冲洗，使用PI染色，随后进行RNA酶处理，最后使用流式细胞仪分析。 (Only for Reference)
动物实验： ^[5]	- 合并 Animal Models: 腹腔注射P388 腹水白血病细胞的雌性Balb/c×DBA/2J F1小鼠,皮下移植A2780, Br-cycE, 或A431细胞的Balb/c nu/nu裸鼠 Dosages: ~7.5 mg/kg/day Administration: 腹腔注射 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	15 mg/mL (37.32 mM)
	Water	Insoluble
	Ethanol	''''8 mg/mL
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 5% DMSO+30% PEG 300+ddH2O	2.5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Flavopiridol (L86-8275) SDF

分子量	401.84
化学式	C₂₁H₂₀ClNO₅
CAS号	146426-40-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	NSC 649890 HCl, HMR-1275, Alvocidib

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03441555	Completed	Drug: Venetoclax\|Drug: Alvocidib	Acute Myeloid Leukemia (AML)	AbbVie\|Sumitomo Dainippon Pharma Oncology Inc	May 30 2018	Phase 1
NCT03298984	Completed	Drug: Alvocidib\|Drug: Cytarabine\|Drug: Daunorubicin	Acute Myeloid Leukemia	Sumitomo Dainippon Pharma Oncology Inc	September 25 2017	Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

研究领域

产品类型

定制您的化合物库

Flavopiridol (L86-8275)

客户使用Selleck生产的Flavopiridol (L86-8275)发表文献85篇：

产品安全说明书

CDK抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Flavopiridol (L86-8275) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

技术支持

CDK Signaling Pathway Map

相关CDK产品