Ramucirumab (anti-VEGFR2)

目录号:A2003 别名: IMC-1121B, LY3009806, IMC-1121 中文名称:雷莫芦单抗

For research use only.
Not for use in humans.

Ramucirumab (anti-VEGFR2) 是IgG1类的单克隆抗体,可与 VEGF-R2 结合并阻止其激活,其阻断KDR与VEGF结合的 IC50 值为0.8 nM;MW: 143.6 KD。

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VEGFR抑制剂选择性比较

生物活性

产品描述 Ramucirumab (anti-VEGFR2) 是IgG1类的单克隆抗体,可与 VEGF-R2 结合并阻止其激活,其阻断KDR与VEGF结合的 IC50 值为0.8 nM;MW: 143.6 KD。
靶点
KDR/VEGF interaction [5]
()
0.8 nM
体外研究

Ramucirumab is direct inhibitor of VEGF-R2, where it binds to the extracellular VEGF-binding domain with high degree of specificity and affinity, at picomolar dose range. It thus prevents the binding of the VEGF ligand to the VEGF-R2 receptor. Ramucirumab has potential advantages over bevacizumab as it is selective for VEGF-R2, whereas bevacizumab by targeting VEGF-A affects VEGF-R1, -R2, and the noncatalytic coreceptors neuropilin-1and -2[1]. Ramucirumab prevents the binding of VEGFR ligands: VEGF-A, VEGF-C, and VEGF-D to its receptors. Thus, ramucirumab inhibits the angiogenesis pathways involved in the development and progression of gastric cancer[2]. ramucirumab demonstrates inhibition of VEGF-stimulated VEGFR-2 activation, proliferation of human endothelial cells, VEGF migration of human leukemia cells, and VEGF induced phosphorylation of VEGFR-2 in human umbilical vein and porcine aortic endothelial cells overexpressing VEGFR-2[3]. Preclinical in vitro data revealed that ramucirumab has a high affinity for VEGFR-2, showing a half-maximal effective concentration (EC50) of 0.15 nM, 8- to 9-fold higher than its natural ligand VEGFA. Ramucirumab binds VEGFR-2 in domain 3 near the N-terminus, both as a soluble protein and as a cell-surface receptor, with a IC50 of 1-2 nM[4].

体内研究 In preclinical studies, ramucirumab concentrations of >20 μg/mL were associated with anticancer activity[1]. And ramucirumab potently inhibited VEGF binding to VEGFR-2 with a binding affinity constant of ramucirumab to VEGFR-2 of 5 × 10-11 M. Pharmacokinetic evaluation has demonstrated a nonlinear pharmacokinetic, with incremental doses of this agent being associated with a decrease in clearance. Ramucirumab has a half-life of 200-300 h[3]. Preclinical studies were also to be conducted in mice, but interspecies receptor differences made ramucirumab inactive in preclinical mouse models[4].

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • The anti-KDR antibodies were added to the microwells of a 96-well plate coated with KDR(Ig1-7) (full-length KDR ECD), KDR(Ig1-3) (variant that contains only the first three N-terminal Ig domains of KDR ECD), or KDR(Ig1) (variant that contains only the first N-terminal Ig domain of KDR ECD) and incubated at room temperature for 1 h. The plate was washed and then incubated with a mouse anti-human Fc antibody-HRP conjugate for additional 1 h, after which the plate was developed as described above. Antibody binding to the two Ig deletion variants are shown as relative (as percentages) to their binding to the full-length KDR ECD.

抗体信息

CAS No. 947687-13-0
配制 PBS buffer, pH 7.2
同型 human IgG1
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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VEGFR Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID