Bevacizumab (anti-VEGF)

目录号:A2006 别名: rhuMab VEGF, Avastin 中文名称:贝伐珠单抗,贝伐单抗

For research use only.
Not for use in humans.

Bevacizumab (anti-VEGF, Avastin) 是一种人源化的anti-VEGF单克隆抗体,是VEGF的抑制剂。可与所有人源VEGF-A亚型(和具有生物活性的蛋白水解片段)结合、中和。MW:149 KD。

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客户使用Selleck生产的Bevacizumab (anti-VEGF)发表文献13篇:

客户使用该产品的1个实验数据:

  • VEGFA expression in the indicated cells treated with or without bevacizumab or transfected with VEGFA or shVEGFA.

    Cancer Lett, 2018, 439:78-90. Bevacizumab (anti-VEGF) purchased from Selleck.

质量管理及产品安全说明书

VEGFR抑制剂选择性比较

生物活性

产品描述 Bevacizumab (anti-VEGF, Avastin) 是一种人源化的anti-VEGF单克隆抗体,是VEGF的抑制剂。可与所有人源VEGF-A亚型(和具有生物活性的蛋白水解片段)结合、中和。MW:149 KD。
靶点
VEGFR2 [4]
()
VEGF-A [4]
()
VEGF [1]
()
体外研究

人源VEGF的Gly88对bevacizumab的结合是基本必需的,同时也保证了bevacizumab结合的种属特异性,大鼠和小鼠的VEGF在该对应位置为丝氨酸残基。bevacizumab可与所有人源VEGF-A亚型(和具有生物活性的蛋白水解片段)结合、中和,而不中和其他VEGF基因家族成员,如VEGF-B或VEGF-C[1]

体内研究

在人体中,bevacizumab的终末半衰期为17-21天。在裸鼠中,Bevacizumab抑制人源肿瘤细胞系的生长,在剂量为1-2 mg/kg(一周给药两次)时,达到最大抑制作用。半最大抑制作用所需剂量为0.1-0.5 mg/kg。在Macaca fascicularis (cynomolgus monkey)中对bevacizumab进行安全性评估研究,对其进行bevacizumab给药4-13周后,年轻成年的食蟹猴骨骺发育不良:肥大的软骨细胞剂量依赖式增多,生长区血管浸润受到抑制。长期bevacizumab的给药抑制了雌性系统的血管生长、卵巢和子宫的体重下降,黄体缺失。生长板和卵巢的改变为可逆性改变,停止治疗可逐渐恢复。除此以外,在药物浓度高达50 mg/kg,也没有其他与给药相关的副作用被观察到[1]。皮下注射后,rhuMAb VEGF(bevacizumab)在大鼠中的生物利用度为69%,而在小鼠和食蟹猴中为100%。Bevacizumab与灵长类VEGF结合,与兔子VEGF低亲和力结合,而不与大鼠、小鼠VEGF结合[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: Effects of bevacizumab on VEGF-induced HUVEC proliferation
    Cells: Human umbilical vein endothelial cells (HUVECs)
    Concentrations: 0~500 ng/mL
    Incubation Time: 96 h
    Method: 50 ng/ml of rhVEGF was pre-incubated with a concentration range of bevacizumab (0-500 ng/ml) for 2 h before added to HUVACs. The plate was continuously incubated for 4 days. The proliferation of cells was determined by using Alamar Blue dye.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/15886877

    Objective: Cell proliferation assay
    Cells: non small cell lung cancer (NSCLC), colorectal cancer (CRC), breast cancer (BC) and renal cell carcinoma (RCC)
    Concentrations: 250 μg/ml
    Incubation Time: 24, 48, 72 or 96 hours
    Method: Between 2 × 103 and 5 × 103 cells/well (cell line/doubling time dependent) are seeded into 96 well plates and incubated overnight to adhere. Medium is then replaced by RPMI-1640 with reduced FBS and bevacizumab or VEGFA at the concentrations indicated (time point zero). After 24, 48, 72 or 96 hours in hypoxia, MTT (5 mg/ml in PBS) is added and incubated for 2 hours at 37°C. The supernatant is removed and reaction products are solubilized for 1 h in 10% HCl, 0.1% NP-40 in isopropanol. Absorbance is measured at 570 nm with a reference wavelength of 650 nm using an ELISA reader. Each experimental condition is analyzed in triplicate and results are an average of a minimum of three biological repetitions.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/24059699

    Bevacizumab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

动物实验:
- 合并
  • Objective: To determine the antitumor response of bevacizumab and cisplatin as single agents or in combination in xenograft models of ovarian cancer
    Animal Models: Nude mice were injected (i.p.) with 5×106 A2780 cells
    Formulation: PBS
    Dosages: 5mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19047105

    Objective: To determine the pharmacokinetics of bevacizumab
    Animal Models: Female nude mice
    Formulation: --
    Dosages: 9.3 mg/kg
    Administration: i.v. or s.c. injection
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/9862791

    Bevacizumab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

抗体信息

CAS No. 216974-75-3
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技术支持

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常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

VEGFR Signaling Pathway Map

相关VEGFR产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID