Cetuximab (anti-EGFR)

目录号:A2000 别名: C225 中文名称:西妥昔单抗

仅限科研使用,禁用于人类

Cetuximab (anti-EGFR)是一种新型的分子靶向药物,是EGFR的抑制剂,同时也是人源EGFR单克隆抗体,与EGFR的胞外结合区域相互作用并抑制其受配体刺激;MW:145.781 KD。

Cetuximab (anti-EGFR)
规格 价格 库存 购买数量
5mg RMB 5790.52 现货
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客户使用Selleck生产的Cetuximab (anti-EGFR)发表文献19

质量管理及产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Cetuximab (anti-EGFR)是一种新型的分子靶向药物,是EGFR的抑制剂,同时也是人源EGFR单克隆抗体,与EGFR的胞外结合区域相互作用并抑制其受配体刺激;MW:145.781 KD。
同型 Human IgG1
靶点
EGFR [4]
(Cell-free assay)
0.39 nM(Kd)
体外研究

Cetuximab treatment increases mitochondrial priming of EGFR-expressing HeLa cells but not in EGFR-expressing MDA-MD-231 cells.[2]

体内研究

在小鼠移植瘤模型中,Cetuximab能够增强几种化疗药物的抗肿瘤活性[1]。它通过多种机制,包括抑制细胞周期的进展,将细胞周期阻滞在G1期、减少S期细胞数目来发挥其抗肿瘤作用。Cetuximab将细胞阻滞在G1期还能引起凋亡,通过诱导和激活相关促凋亡分子。单独给药或是与carboplatin协同给药能够引起肿瘤大小减少、转移扩散减少、减少细胞表面表达EGFR、无BRAF和K-ras突变的NCI-N87肿瘤中MVD。而在源自于MKN-45的肿瘤中作用甚微(其肿瘤表型是BRAF和K-ras行盛行,但胞质内EGFR弱表达)[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
  • Cells were lysed at a density of 1 x 106/50 μL in lysis buffer (0.25 M Tris-HCl, 2% sodium dodecylsulfate, 4% β-mercaptoethanol, 10% glycerol, 0.02% bromophenol blue) supplemented with 1 X protease/phosphatase inhibitor cocktail. Cell lysates were then loaded onto polyacrylamide gels with sodium dodecyl sulfate. After electrophoresis, proteins were transferred to polyvinylidene difluoride (PVDF) membranes. The transblotted membranes were blocked for 1 hr and then probed with appropriate primary antibodies overnight at 4 ℃. Next day, the membranes were washed three times for a total of 30 min and then incubated with IRDye 680RD Donkey anti-Rabbit IgG (H + L) or IRDye 800CW Donkey anti-Mouse IgG (H + L) in darkness at room temperature for 1 h. After another three washes, scan immunoblot membranes and quantify band intensity.

动物实验:
  • Objective: Cetuximab在小鼠胃癌模型中的抗肿瘤活性
    Animal Models: 人类胃癌的裸鼠模型(CD-1/nu-nu mice)
    Formulation: 0.9% NaCl
    Dosages: 1 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/22011788

    Objective: To investigate the relationship between the EGFR levels and the responsiveness to cetuximab treatment in human cancer xenograft models
    Animal Models: Female athymic, nude mice were implanted s.c. with ~1 mm3 tumor fragments
    Formulation: PBS
    Dosages: 0.25, 0.5 or 1 mg/mouse
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27186886

    Cetuximab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

抗体信息

CAS号 205923-56-4
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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常见问题及建议解决方法

问题 1:
How to store the antibody?

回答:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.