3-Methyladenine (3-MA)

目录号:S2767 别名: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA)是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。现配现用(加热助溶)

规格 价格 库存 购买数量  
RMB 579.66 现货
RMB 3010.37 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck该产品发表文献10篇:

客户使用该产品的6个实验数据:

  • granulosa cells (GCs) with 24 h of melatonin (10 μM) treatment were rinsed in PBS, and then exposed to H2O2 (200 μM) for 2 h. The autophagy inhibitor 3-MA (10 mM), or the apoptosis inhibitor Z-VAD-FMK (50 μM) were added 1 h prior to H2O2 incubation. Cell viability was determined using the CCK-8 assay. Data represent mean ± S.E; n = 3 in each group. *P < 0.05 (**P < 0.01) vs. vehicle group at 0 h. # Represents P < 0.05 (## Represents P < 0.01) vs. H2O2-only-treated cells. & Represents P > 0.05 vs. H2O2-only-treated cells. N, not significant, P > 0.05. δ Represents P < 0.05 (δδ Represents P < 0.01) vs. Z-VAD-FMK-treated cells.

    Redox Biol, 2018, 18:138-157. 3-Methyladenine (3-MA) purchased from Selleck.

    MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

  • Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

    PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

  • After getting treated with 10 μM compound C for indicated time periods with or without 50 μM 3-MA (C) pre-treatment for 1 h, the levels of indicated proteins in QBC939 and RBE cells were analyzed using Western blot.

    J Cell Biochem, 2017, 119(7):5538-5550. 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 3-Methyladenine (3-MA)是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。现配现用(加热助溶)
靶点
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
体外研究

3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的[1] 。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵,这表明3-MA 除了抑制自吞噬之外还有其它生物功能。3-MA可以造成半胱天冬酶依赖的细胞死亡,这一功能与它对自吞噬的抑制无关。用5mM 3-MA处理葡萄糖饥饿Hela细胞可降低gfp-lc3阳性细胞比例至23%。3-MA处理细胞12到48小时内 LC3-I 的水平上升而LC3-II 水平下降。LC3-I转为为 LC3-II 的过程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 处理HeLa 细胞一天并不影响细胞存活率, 而 10 mM 3-MA 处理一天会造成细胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 处理细胞两天分别使细胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低细胞存活率的作用具有时间和剂量依赖特性。3-MA 明显缩短nocodazole诱导的前中期阻断时间[2]。3-MA 通过抑制自吞噬来抑制SU11274诱导的细胞死亡[3]。 在野生型MEF细胞中延长 3-MA处理时间 (最多 9 小时) 明显造成 LC3 I 到 II 转换。延长 3-MA处理时间会明显增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介导的LC3 转换和游离 GFP 释放是ATG7依赖的。 3-MA处理会导致 p62 蛋白水平明显升高。即使在Atg5−/− 的MEF细胞中3-MA 也会使p62 水平升高,就像在DOX介导的ATG5缺失的细胞中一样 。3-MA以不同方式抑制 I 型和 III型 PI3K。与野生型细胞相比,在Tsc2−/−细胞中3-MA诱导的 LC3 I 到 LC3 II 转换过程大幅下降。3-MA会破坏mTOR复合物1的拮抗自吞噬功能[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D Mm\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXKzcW0> NX7zd|BuOmh? Ml3KSG1UVw>? Ml:3doVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? MlzRNlU4OTZ3NkG=
A549  NY[x[lVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHFNYg{dU1? NGPaNHYzcA>? MnnuSG1UVw>? MXry[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E NUT0XZIxOjV5MU[1OlE>
95D Mmm5RZBweHSxc3nzJGF{e2G7 NHPVOoQ2dU1? MnnUNog> M1XwU2ROW09? MnK3bY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq M4HEbFI2PzF4NU[x
A549  MYDBdI9xfG:|aYOgRZN{[Xl? MmmyOY1O M1fucVJp NUDmW5oyTE2VTx?= M1fQNYlvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= NUTMV5c1OjV5MU[1OlE>
NBL-W-S  MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfYU2FKOW2P MkHCOog> M{XDXIVvcGGwY3XzJGdCVlRvNkGgeI95cWOrdIm= M2\VNVI2OzJ|MkKy
NBL-W-S  NFy1UmxCeG:ydH;zbZMhSXO|YYm= MX[xcW0> MojOOog> Mo\JbY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= NIXJeYUzPTN{M{KyNi=>
A2780cp  MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HDTFFuVQ>? MYWxbC=> M{fRVYlv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp MoTYNlU{OjJ4OUS=
A549  NYexclFlTnWwY4Tpc44hSXO|YYm= MnzBOY1O NITkSHUzcA>? NYXweZE2e3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK MlWwNlUzQDV4Mki=
H157 NHrYcXNHfW6ldHnvckBCe3OjeR?= NWPwdXNxPW2P NW\mc2NGOmh? NX:5OZdle3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK Mn23NlUzQDV4Mki=
MDR MWHBdI9xfG:|aYOgRZN{[Xl? NFexe|IyOG2P MXO2bC=> M{CxXJN1emWwZ4To[Y5{KHSqZTDwc5dmeiCxZjDhcpRq[2GwY3XyJIRzfWe| MoD1NlUxOTl5MEG=
HT-29  NIP2bm1CeG:ydH;zbZMhSXO|YYm= MXexcW0> M2nLclQ5cA>? M3PETmROW09? M1;wSYVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NHKwcIozPDh2MkG1PC=>
Microglia  NGLDUYlCeG:ydH;zbZMhSXO|YYm= MXK1cW0> NFztT3EzPGh? Mn3p[IVkemWjc3XzJIh6eG:6aXGtbY5lfWOnZDDj[YxtKGSnYYTo MVqyOFgyQDZyMR?=
A2780cp NVewXYVtSXCxcITvd4l{KEG|c3H5 NYL3e2I2Oi53bV2= MYmxbC=> M4HvOIRlUDKR NWfVOYJn\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NYDFcGdyOjR6MUe5OFY>
HepG2 MV\GeY5kfGmxbjDBd5NigQ>? MmHYOY1O NGe2U|Y1cA>? MXfpcoNz\WG|ZYOgTGwhemWuZXHz[S=> NYLGN5poOjR5MUO1PFc>
A549 MmjIRZBweHSxc3nzJGF{e2G7 M3S4VFVuVQ>? NEjRVmE1QGh? M2npeoRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuIHTlZZRpKGGwZDDlfJBz\XO|aX;uJIxmfmWuczDv[kBk[XOyYYPlMVMtKEKnY3zpck0yKGGwZDDMR|MuUUl? M4nEZlI1PzB4M{Cz
U2OS M1vOemFxd3C2b4Ppd{BCe3OjeR?= Mn\WNVBuVQ>? NHW1O3ozPGh? MYfpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> M2npflI1PjN7MEGz
Saos-2 Mn[3RZBweHSxc3nzJGF{e2G7 NH\5WFAyOG2P MoXwNlRp NXnaZpB1cW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 NYO4bpFPOjR4M{mwNVM>
A549 NHTR[phCeG:ydH;zbZMhSXO|YYm= MV:xNI1O MXu0PIg> MmfoZYNk\WyncnH0[ZMhfGinIILl[JVkfGmxbjDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JHBVYA>? MknKNlQ3OjZ5MkK=
HCT116  MWPBdI9xfG:|aYOgRZN{[Xl? M4HWeFVuVQ>? M4XwfVI1cA>? MWjlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? MkHTNlQ3OjZ3MkK=
HGC-27 Mkm2SpVv[3Srb36gRZN{[Xl? M3jQelExdU1? M1L0c|Fp M13obIlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 M2DrfFI1PDF4M{S5
U2OS  NHvydFNHfW6ldHnvckBCe3OjeR?= M{PVeFAvPS9zbV2= M4\aSlQ5cA>? MYXpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> Mn23NlQ{PTh|NEK=
MG-63 MV;GeY5kfGmxbjDBd5NigQ>? NHLKeIwxNjVxMX3N NUDPT|d4PDiq MljBbY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> MnSzNlQ{PTh|NEK=
MG-63 MkfFRZBweHSxc3nzJGF{e2G7 NH7kc|UxNjVxMX3N NUTVNlROOzKq M4CyR4VvcGGwY3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= Mof0NlQ{PTh|NEK=
MG-63 NWrnRoU4SXCxcITvd4l{KEG|c3H5 MYOxNI1O MV6xNog> MkPz[Y5p[W6lZYOgSHAucW6mdXPl[EBieG:ydH;zbZM> Mnj4NlQ{PTh|MEG=
NTUB1 M3jKVGFxd3C2b4Ppd{BCe3OjeR?= MXe1cW0> MW[xMlVp M{TYVZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NUTrRpI4OjR|NEmxO|Y>
T24 M4fKTWFxd3C2b4Ppd{BCe3OjeR?= MVe1cW0> NV3jW4g{OS53aB?= NYHYTJR7eG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| M33U[lI1OzR7MUe2
SGC-7901  NIPvWJdCeG:ydH;zbZMhSXO|YYm= MVyycW0> MVSxbC=> M4TtV4lv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| NWTEbmEyOjR|MkGzOFA>
SMMC-7721 MXfBdI9xfG:|aYOgRZN{[Xl? MnXXNo1O NXvVXm43OWh? NHPXVnRqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= NI\FPWMzPDN{MUO0NC=>
ECSCs  NHS5XWdCeG:ydH;zbZMhSXO|YYm= M4jZR|ExdU1? NYfjOVdvPGh? M1T5e4Rm[3KnYYPld{Bz[XCjbYnjbY4ufHKnYYTl[EBieG:ydH;zbZM> NEfUW4wzPDNzOUGwPS=>
MCF-7  NFfHb5BHfW6ldHnvckBCe3OjeR?= NHflSpgyOG2P M4juc|I1cA>? NWSxXmpGcW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JINp\W2xdHjldoFxgSCmcoXndy=> NFvm[pUzPDNzNUW3PC=>
UOK257 M{G2WmFxd3C2b4Ppd{BCe3OjeR?= MYe1cW0> Mnq0N4g> MWflcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh M1GxblI1OzB3NkC0
ACHN-5968 M37udGFxd3C2b4Ppd{BCe3OjeR?= MnewOY1O NFq3d40{cA>? NXXCU4Ft\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= MmDvNlQ{ODV4MES=
Huh7 NYrhWIRLSXCxcITvd4l{KEG|c3H5 NGHjNVIzdU1? MmHaNVJp Ml:zSG1UVw>? MVnpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= MV2yOFI6PzNyMB?=
Hep3B M1rnd2Fxd3C2b4Ppd{BCe3OjeR?= NFuwOmQzdU1? MYmxNog> NGrjcpNFVVOR NXjhd4l4cW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> MnrBNlQzQTd|MEC=
RKO MkD2SpVv[3Srb36gRZN{[Xl? M{HQcFJuVQ>? M3zCPVFp NIL5ZndFVVOR NUTiXVRo\W6qYX7j[ZMh[2WubDDk[YF1cCCkeTDn[Yxl[W6jbYnjbY4> M{S1TFI1OjlzN{e3
HepG2 E47 NVL6emM1TnWwY4Tpc44hSXO|YYm= NIDBbJYzNjWvTR?= NVrrZoo1PDiq MXPpcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 NIPueWwzPDJ5M{ezPC=>
LoVo  MmfuRZBweHSxc3nzJGF{e2G7 NFzIR5A2dU1? M1jHZ|Q5cA>? NGTqOmlmdmijbnPld{BFS0FvaX7keYNm\CCjcH;weI9{cXNw MWiyOFIxOThzMh?=
WiDr NGryRWxHfW6ldHnvckBCe3OjeR?= MWSxNI1O NYS4U|RWOWh? MlLkbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v MXmyOFE6ODR6OR?=
H1299  M1y0ZWZ2dmO2aX;uJGF{e2G7 MkS0NVBuVQ>? MofiOIg> NVWwRldGcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? NEnsXXAzPDF5M{KwPC=>
H460 MXHGeY5kfGmxbjDBd5NigQ>? MU[xNI1O NGfaOmQ1cA>? NXHMeXROcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? MnnoNlQyPzN{MEi=
A549 Ml35SpVv[3Srb36gRZN{[Xl? MXWxNI1O MVS0bC=> M1fpdYlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w MVKyOFE1Ojd|NR?=
Hep3B M1LaU2Fxd3C2b4Ppd{BCe3OjeR?= MnjqOY1O NUXXenhZOjSq MmLMZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NVrDW4hoOjRyNk[2PVM>
SMMC-7721 M3vTXmFxd3C2b4Ppd{BCe3OjeR?= NGrhUGk2dU1? NYPJdIdbOjSq NIL2fYRifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> NILa[VEzPDB4Nk[5Ny=>
HO8910 MoG5RZBweHSxc3nzJGF{e2G7 MYKxNI1O M{LrR|EzcA>? MVXlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> MWWyN|k5OzZzMB?=
MCF7 M4\hbmZ2dmO2aX;uJGF{e2G7 MkHDOY1O M4mydVI1cA>? MmXubY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? M2Dk[lI{QTZ{NkK5
HONE-1  MV7GeY5kfGmxbjDBd5NigQ>? MVW1cW0> NEnK[2gycA>? NGrBb2Zz\XC{ZYPz[ZMhPnJvbXXkbYF1\WRiUl;TJJBzd2S3Y4Tpc44> M1rvVFI{QDl{M{W4
HeLa M3raUGZ2dmO2aX;uJGF{e2G7 M1zVelExdU1? NWTHcYc{Omh? NUXSVJVQe3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? NUTZT3BiOjN6NkS3N|g>
HepG2 MXHGeY5kfGmxbjDBd5NigQ>? NF;aTXcyOG2P NWqyc|lbOjSq M2HIUIlvcGmkaYTzJJNqXEmJQWKtJIFv\CCKQmPTMYlv\HWlZXSgZZV1d3CqYXf5 NXmyeVlFOjN6MUewOFA>
SH-SY5Y NYfQfJRpTnWwY4Tpc44hSXO|YYm= NYq4[4lxOW2P MoW2NlRp MXPpcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF;DVC=> M33mV|I{PzR|MUS4
SH-SY5Y MofiRZBweHSxc3nzJGF{e2G7 NW\XWnlSPW2P NUTaO3FwOWh? NXfyWIxF[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 MX2yN|YyQTN7NR?=
PC12  MUXGeY5kfGmxbjDBd5NigQ>? M37tclExdU1? M3HUUlI1cA>? NV3KZXh5f2G2ZYK= MUFCpIlvcGmkaYTzJINpgW2xdIL5dJNqdi2uaXvlJJBzd3SnYYPvcYFtKGGldHn2bZR6Ng>? MVqyN|YxOzl5OR?=
OV2008 MVTBdI9xfG:|aYOgRZN{[Xl? NGHkVIc2dU1? NX\IU4tTOjSq M1zw[YNwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| M3jDd|I{PTl{Mkix
A2780 NXS3TpM2SXCxcITvd4l{KEG|c3H5 MVi1cW0> NH:yW3kzPGh? MY\jc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? M{HZb|I{PTl{Mkix
Saos-2  NFu1c2hCeG:ydH;zbZMhSXO|YYm= NY\oRZlkOW2P M3HZblk3cA>? NEPVbGFqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IGDDXC=> NVLxUoV{OjN3NkOxO|E>
1321N1 MmT5R5l1d3SxeHnjbZR6KEG|c3H5 NWDXW3JxPW2P MVyyOIg> MoH4dJJwfGWldIOgZ4VtdCCjZ3HpcpN1KFCFTj3pcoR2[2WmIITvfIlkcXS7 NWnJWWF[OjN3MkWyOlU>
SH-SY5Y MkfvR5l1d3SxeHnjbZR6KEG|c3H5 M3yyOlVuVQ>? NGfNVGEzPGh? NIP5SWJqdmO{ZXHz[ZMhWEOQIITvfIlkcXS7 M3G0dFI{PTJ3Mk[1
HT-29  NHzXUVRHfW6ldHnvckBCe3OjeR?= NVGzO|VuOW2P M4DpV|Q5Nzl4aB?= MVTpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq NUTlTJZtOjN3MEiyO|I>
OR6 NUTQWVZYTnWwY4Tpc44hSXO|YYm= NV[2[5JwOTCvTR?= NHLCZmo4Omh? M33J[JN2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN? NF7WeoEzOzN7NUi3OS=>
Hela  MnzxSpVv[3Srb36gRZN{[Xl? Mnz0OY1O NHvDfWIzPGh? MX\pcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 MW[yN|M6PTZ5OR?=
MCF-7  MWnGeY5kfGmxbjDBd5NigQ>? M2fCRlVuVQ>? NIjzU2gzPGh? M4DMNIlvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> M2LYclI{Ozl3Nke5
HUVECs MoDmSpVv[3Srb36gRZN{[Xl? MXezcW0> M{XwdFI1cA>? NIntO3ZjdG:la4OgeIhmKHC{b4TlZ5RqfmViZX\m[YN1KG:oIILld5ZmemG2cn;sJIJ6KGmwaHnibZRqdmdiYYX0c5Bp[We7 NVzGPHRqOjN|NUi5Nlg>
T24 MljaSpVv[3Srb36gRZN{[Xl? NFTreo8yOG2P M363XFFp MmfJdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= NX71OGZPOjN|NUSwPFA>
U251MG  NF3Zc4lHfW6ldHnvckBCe3OjeR?= NWHqcWVqO22P MVKxbC=> NYmyZnp1e3WycILld5NmeyCOQ{OtTWkheHKxdHXpckBmgHC{ZYPzbY9v NVHB[IR{OjN|M{i2NVg>
GTL-16  MkDORZBweHSxc3nzJGF{e2G7 M3;Be|VuVQ>? NGW3bXozPGh? NVnqTWk{emWmdXPld{Bk\WyuII\pZYJqdGm2eTDhd{Bkd22yYYLl[EB1dyClZXzsd{B1emWjdHXkJJdqfGhiTVXUJIlvcGmkaYTvdpM> M1vWPVI{OzF|NEmw
T-47D MUfGeY5kfGmxbjDBd5NigQ>? NHfxcZcyOG2P Mn6zNog> NHiyVY5qdmirYnn0d{BifXSxcHjh[5kheHKxY3Xzd{BidmRiaX7jdoVie2W|IILhdIFugWOrbjDpcoR2[2WmIHHwc5B1d3Orcx?= MXmyN|MxODB{Nh?=
PaCa44 NXPoVpMzSXCxcITvd4l{KEG|c3H5 NUHzWY5IOi53bV2= MoXRNYg> NV3peocxemWmdXPld:Kh\2WwaYDpck1u\WSrYYTl[EBieG:ydH;zbZM> MXOyN|EzPDFzMh?=
MDA-MB231 MnzESpVv[3Srb36gRZN{[Xl? NIC3N|U2dU1? MVixbC=> M{DtNolv[3KnYYPld{Bz\XO4ZYLheJJwdC2vZXTpZZRm\CClYYPwZZNmKGGldHn2ZZRqd25iYX7kJINmdGxiZHXheIg> NHzZPHUzOzB6OEi1NC=>
HeLa MYLBdI9xfG:|aYOgRZN{[Xl? MoDqNVBuVQ>? MmDnNog> MnfS[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2bXXueEB4cXSqIGDFTS=> NYfCRZdrOjNyMECxN|U>
SK-HEP-1 MWjBdI9xfG:|aYOgRZN{[Xl? MX[xNI1O MYCxbC=> NXHC[Yx1eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? M{PXd|IzQDV6NkS5
HepG2 M2DNemFxd3C2b4Ppd{BCe3OjeR?= NGrGVHA{dU1? MUe1bC=> NHnzWHFz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgVWR{ MnfINlI5OzZ3OUW=
MCF-7  M2TCTGZ2dmO2aX;uJGF{e2G7 MWGycW0> NEHpOlczPGh? MWjpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi MXuyOFk4ODZ5Nh?=
MDA-MB-231 NXSxenVYTnWwY4Tpc44hSXO|YYm= Mmn3No1O NFLVcpMzPGh? NFjWcI9qdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg MXyyOFk4ODZ5Nh?=
MCF-7  MkH1SpVv[3Srb36gRZN{[Xl? M37kXFJuVQ>? NIKwXJE1QGh? M4H1UJBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? MonpNlQ6PzB4N{[=
MDA-MB-231 MYfGeY5kfGmxbjDBd5NigQ>? NULqTpF3Om2P MkXsOFhp MWTwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> NXiwRmZ{OjR7N{C2O|Y>
PANC-1  NXrI[ZJCSXCxcITvd4l{KEG|c3H5 M1L3SFFuVQ>? MYe0PIg> Moe5SG1UVw>? NH;GcXNmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NVLmdYtXOjR6NEKxOVg>
MDA-MB 231 NGDOPZdCeG:ydH;zbZMhSXO|YYm= NILLPYM2dU1? MnXXNE42cA>? MYPtc4R2dGG2ZYOgWI9kd22rbtMuxsBqdmS3Y3XkJIFxd3C2b4Ppdy=> NH;tS5UzPDh|MEe4NS=>
Microglia  M{PXU2Fxd3C2b4Ppd{BCe3OjeR?= MUO1cW0> MUGyOIg> NH75V2xl\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> MomxNlQ5OTh4MEG=
A2780cp NUPuc4g3SXCxcITvd4l{KEG|c3H5 MkmxNk42dU1? M4HSelFp NIPE[Wll\Eh{Tx?= NILSXYpmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp M1P3UlI1QDF5OUS2
HepG2 MkXtSpVv[3Srb36gRZN{[Xl? MXi1cW0> Mnj4OIg> MYnpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? MnHENlQ4OTN3OEe=
U2OS MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXSZZAyOG2P Ml7iNlRp MmrDbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgSI95 NHHybXAzPDZ|OUCxNy=>
HCT116 NWDnWGpYSXCxcITvd4l{KEG|c3H5 MkjsOY1O MmH6NlRp NUXyUohrTE2VTx?= NILxfm5mdmijbnPld{BieGmpZX7pck1qdmS3Y3XkJINmdGxiZHXheIg> MVeyOFYzPjV{Mh?=
MCF-7  MlO2RZBweHSxc3nzJGF{e2G7 NGDlN3oxNjGvTR?= MYG2bC=> NHXPUJBmdmijbnPld{B{cXK2aX7vcE1qdmS3Y3XkJIFxd3C2b4Ppdy=> MnfWNlI4PTF7OEm=
PC-3  M3f4T2Fxd3C2b4Ppd{BCe3OjeR?= NFvZXGczdU1? NFnhZY0zcA>? M1K2d4lv[3KnYYPld{BQWklvaX7keYNm\CClZXzsJIRm[XSq MUOyNlc1PTV6MB?=
U251  M3vJdWFxd3C2b4Ppd{BCe3OjeR?= MoLrOY1O M1\Hc|I1cA>? NXnCe2RVcW6lcnXhd4V{KFNzLXnu[JVk\WRiY3XscEBl\WG2aB?= NV36[pN7OjJ3N{m3PFg>
HeLa  NFHjXVRCeG:ydH;zbZMhSXO|YYm= M13YNFVuVQ>? MYqyOIg> NXrTdZhzcW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp M2TYUFIzPTR3MUK4
A549 M3S4UWZ2dmO2aX;uJGF{e2G7 M3vFW|AvOW2P M2fKOFI1cA>? MV7zeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo MUGyNlQ3Pjl4MB?=
pDCs M1LjSmZ2dmO2aX;uJGF{e2G7 MWOxNI1O MUCwMlVp NFP3RpbDqHKnZIXj[ZMhfGinIHnu[JVkfGmxbjDv[kBKTk5vzsGgZpkhe3OUTlG0NC=> NUPId5B5OjJ|OU[1PVk>
BGC-823 M1LxNGZ2dmO2aX;uJGF{e2G7 MofIOY1O NYLYUoFWOmh? M4C0RYlvcGmkaYTzJJRp\SC{YYTlJI9nKGG3dH;wbIFocWNiY3XscJM> M4TLcFIzOzJ{MUWy
U937 M2\0eWZ2dmO2aX;uJGF{e2G7 NIHvOXUzdU1? MYGxNog> M{\Ld4Rm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MnXFNlIyPTVzNUC=
Marc-145 MmPySpVv[3Srb36gRZN{[Xl? MnjDOY1O NILVTIwyOi9{ND:zOog> MkTIdoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w NGDGSFkzOjFzOUmwNC=>
HBx  MnPGRZBweHSxc3nzJGF{e2G7 NEDOe4EyOG2P NV75WpBoPDiq NUXNO4hmTE2VTx?= MljibY5kemWjc3XzJINmdGxiZHXheIg> NHHPTIUzOjB{MEC3PC=>
MCF-7 NU\nNphPTnWwY4Tpc44hSXO|YYm= M2q1NlExdU1? MoPrOFhp M2Hpd4Jtd2OtczDheZRweGijZ4mgbY5lfWOnZDDifUBjd3K2ZYrvcYlj M3jtbVIyQTNzOUO3
RMPI8226  MknOSpVv[3Srb36gRZN{[Xl? Mo[yOY1O Mm\JNYg> MUDzeZBxemW|c3XzJJRp\SCuZY\lcEBw\iCjdYTvdIhi\3lidX7k[ZIhdnW2cnnlcpQh\GWybHX0bY9v MVKyNVkyPTZ{MB?=
PC12/TetOn MWHGeY5kfGmxbjDBd5NigQ>? MXGwMlEwOW2P MX2xPIg> M4HpVYxm[WS|IITvJO6yNXO7bjjXWEkh[WOldX31cIF1cW:wLDD0c5hq[2m2eTygZY5lKG:uaXfvcYVzKG[xcn3heIlwdsLi MUCyNVkxPjZ3OR?=
HeLa  MYjDfZRwfG:6aXPpeJkhSXO|YYm= M12ycFJuVQ>? NGDsN5czPGh? NEDse|RqdmirYnn0[ZMhfGinIHP5eI91d3irY3n0fUBw\iC|aXzpZolvcW5idH:gTIVN[SClZXzsd{4> NHfkSnMzOTh5NUO4OS=>
Jurkat NFqzOHlHfW6ldHnvckBCe3OjeR?= NIjGXHIyOG2P MorTNYg> NHLXcm1l\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz MXuyNVg3PDB|Nx?=
K562 MWjGeY5kfGmxbjDBd5NigQ>? NWPv[GhSOTCvTR?= M17SW|Fp M1\idIRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= MVyyNVg3PDB|Nx?=
Nara-H MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXq0ToxmPW2P NX7vZ|NMPDiq MXTlcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w M1vae|IyQDB3MEOz
HUVECs NIT4W5VHfW6ldHnvckBCe3OjeR?= MnK2NVBuVQ>? NE\jZYcxNjWq NHPNR4Jl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l NFnMXoIzOTR4OEW5Ni=>
HepG2 MUfBdI9xfG:|aYOgRZN{[Xl? MkjGNo1O MWqxbC=> M{i2foVvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxiZHXheIg> NGjrU48zOTR3M{[5NS=>

... Click to View More Cell Line Experimental Data

体内研究 3-Methyladenine (3-MA)可以通过对磷酸肌醇3磷酸激酶(PI3K) 的作用来阻断自吞噬, 而PI3K的活性对于自噬体形成早期膜池的成核和组装是必须的。与SAH 处理组相比3-MA并不会改变出血的程度。与SAH +对照成分组相比经过3-MA预处理后会明显加重神经病学症状。3-MA处理会减少自吞噬发生[5]。 相反地,在SAH + 3-MA组里 断裂的半胱天冬酶表达量明显上调,与此一致的是与SAH + 对照成分组相比,SAH + 3-MA组中右脑皮层里原位末端标记阳性细胞数量明显增多[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[4]
+ 展开

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
细胞实验:[2]
+ 展开
  • Cell lines: HeLa 细胞系
  • Concentrations: 1-10 mM
  • Incubation Time: 3 分钟
  • Method: 用台盼蓝染色法分析细胞存活率(如HeLa细胞)。简言之, 经过3-MA处理后将贴壁的与漂浮的细胞都用PBS( pH 7.4)重悬,密度控制在每毫升1-2×106。加入等体积的0.4% 台盼蓝并彻底混匀。室温孵育3分钟后用血细胞计数器对细胞计数分析。
    (Only for Reference)
动物实验:[5]
+ 展开
  • Animal Models: 体重 300-350 g 的成年雄性Sprague–Dawley 大鼠
  • Formulation: 生理盐水配制
  • Dosages: 400 nM
  • Administration: 脑室给药
    (Only for Reference)

溶解度 (25°C)

体外 Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 149.15
化学式

C6H7N5

CAS号 5142-23-4
稳定性 powder
in solvent
别名 NSC 66389

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • 回答:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

相关PI3K产品

Tags: 购买3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)供应商 | 采购3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)价格 | 3-Methyladenine (3-MA)生产 | 订购3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID