3-Methyladenine (3-MA)

For research use only. Not for use in humans.

目录号：S2767 别名： NSC 66389 中文名称：3-甲基腺嘌呤

3-Methyladenine (3-MA) Chemical Structure

CAS No. 5142-23-4

3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，在 HeLa细胞中IC50分别为25 μM和60 μM；永久性抑制I型PI3K，但对III型PI3K的抑制是短暂的，也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用（加热助溶）。

规格 价格 库存 购买数量  
RMB 579.66 现货
RMB 3010.37 现货
有超大折扣
大剂量询单有大折扣!

今日订购，明日送达，全国免运费！

全国免费电话：400-668-6834   |   Email：info@selleck.cn

客户使用Selleck生产的3-Methyladenine (3-MA)发表文献532篇：

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，在 HeLa细胞中IC50分别为25 μM和60 μM；永久性抑制I型PI3K，但对III型PI3K的抑制是短暂的，也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用（加热助溶）。
靶点
Vps34 [1]
(HeLa cells)		 PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
体外研究

3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的[1] 。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵，这表明3-MA 除了抑制自吞噬之外还有其它生物功能。3-MA可以造成半胱天冬酶依赖的细胞死亡，这一功能与它对自吞噬的抑制无关。用5mM 3-MA处理葡萄糖饥饿Hela细胞可降低gfp-lc3阳性细胞比例至23%。3-MA处理细胞12到48小时内 LC3-I 的水平上升而LC3-II 水平下降。LC3-I转为为 LC3-II 的过程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 处理HeLa 细胞一天并不影响细胞存活率, 而 10 mM 3-MA 处理一天会造成细胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 处理细胞两天分别使细胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低细胞存活率的作用具有时间和剂量依赖特性。3-MA 明显缩短nocodazole诱导的前中期阻断时间[2]。3-MA 通过抑制自吞噬来抑制SU11274诱导的细胞死亡[3]。 在野生型MEF细胞中延长 3-MA处理时间 (最多 9 小时) 明显造成 LC3 I 到 II 转换。延长 3-MA处理时间会明显增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介导的LC3 转换和游离 GFP 释放是ATG7依赖的。 3-MA处理会导致 p62 蛋白水平明显升高。即使在Atg5−/− 的MEF细胞中3-MA 也会使p62 水平升高，就像在DOX介导的ATG5缺失的细胞中一样 。3-MA以不同方式抑制 I 型和 III型 PI3K。与野生型细胞相比，在Tsc2−/−细胞中3-MA诱导的 LC3 I 到 LC3 II 转换过程大幅下降。3-MA会破坏mTOR复合物1的拮抗自吞噬功能[4]。
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 NXLtemlnTnWwY4Tpc44hSXO|YYm= M1vpclExdU1? MUSxbC=> NUjYb4Y2\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> NWTTXoc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OlQxOzdpPkKxPFY1ODN5PD;hQi=>
Jurkat NHvROG9HfW6ldHnvckBCe3OjeR?= NIfUTpkyOG2P M4rQXlFp NEK4V4Nl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz Mnj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NkSwN|coRjJzOE[0NFM4RC:jPh?=
HeLa  NH[wcnVEgXSxdH;4bYNqfHliQYPzZZk> NVTLb|FSOm2P NGe0RnQzPGh? M2qwdIlvcGmkaYTld{B1cGViY4n0c5RwgGmlaYT5JI9nKHOrbHnibY5qdiC2bzDI[WxiKGOnbHzzMi=> NFTibVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3OVM5PSd-MkG4O|U{QDV:L3G+
PC12/TetOn Mn3oSpVv[3Srb36gRZN{[Xl? Ml7SNE4yNzGvTR?= NUe5coxZOTiq Ml;JcIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? Mn7WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7ME[2OVkoRjJzOUC2OlU6RC:jPh?=
RMPI8226  NXHxOZEyTnWwY4Tpc44hSXO|YYm= M4TiUFVuVQ>? MWOxbC=> MYHzeZBxemW|c3XzJJRp\SCuZY\lcEBw\iCjdYTvdIhi\3lidX7k[ZIhdnW2cnnlcpQh\GWybHX0bY9v MofVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7MUW2NlAoRjJzOUG1OlIxRC:jPh?=
MCF-7 MWXGeY5kfGmxbjDBd5NigQ>? MVmxNI1O MY[0PIg> NFjiNJRjdG:la4OgZZV1d3CqYXf5JIlv\HWlZXSgZpkh[m:{dHX6c41q[g>? NXrY[lZ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5N|E6OzdpPkKxPVMyQTN5PD;hQi=>
HBx  MnrURZBweHSxc3nzJGF{e2G7 M2nZSlExdU1? NWm2bnhHPDiq MVTEUXNQ NF\vZXBqdmO{ZXHz[ZMh[2WubDDk[YF1cA>? M1Ps[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKwNFc5Lz5{MkCyNFA4QDxxYU6=
Marc-145 Mn;vSpVv[3Srb36gRZN{[Xl? MlX6OY1O NXHIZVJ2OTJxMkSvN|Zp M3XMOZJm\HWlZYOgeIhmKFCUUmPWJJRqfGW{czDhcoQhfGinIIDyc5RmcW5iZYjwdoV{e2mxbh?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937 NGPrRpVHfW6ldHnvckBCe3OjeR?= MmjINo1O NUjUT4JFOTKq NXzl[VRP\GWlcnXhd4V{KHSqZTDheZRweGijZ4mgdoF1cW0EoB?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF3NUG1NEc,OjJzNUWxOVA9N2F-
BGC-823 Mmq0SpVv[3Srb36gRZN{[Xl? NXu0OIZ[PW2P MWqybC=> NVL5OZBqcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? MnHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MkKxOVIoRjJ{M{KyNVUzRC:jPh?=
A549 NF\ncVdHfW6ldHnvckBCe3OjeR?= M2KyblAvOW2P NFjDWHkzPGh? NVzQb2pWe3WycILld5NmeyCVVUGxNlc1NWmwZIXj[YQh[2WubDDk[YF1cA>? M2TrO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[2PVYxLz5{MkS2Olk3ODxxYU6=
pDCs M2i1WmZ2dmO2aX;uJGF{e2G7 NVTM[YNROTCvTR?= NXPt[FNwOC53aB?= NWLtZ2J2yqC{ZXT1Z4V{KHSqZTDpcoR2[3Srb36gc4YhUU[QLd8xJIJ6KHO|Ul7BOFA> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN7NkW5PUc,OjJ|OU[1PVk9N2F-
HeLa  M33iU2Fxd3C2b4Ppd{BCe3OjeR?= M3zIXFVuVQ>? MkfiNlRp MoHTbY5lfWOnczDjZZNx[XOnLXTldIVv\GWwdDDj[YxtKGSnYYTo NVPDZplpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFUyOjhpPkKyOVQ2OTJ6PD;hQi=>
U251  NH\4XJBCeG:ydH;zbZMhSXO|YYm= MUC1cW0> MWGyOIg> MY\pcoNz\WG|ZYOgV|EucW6mdXPl[EBk\WyuIHTlZZRp NYH0OXI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1O|k4QDhpPkKyOVc6Pzh6PD;hQi=>
MCF-7  MV\BdI9xfG:|aYOgRZN{[Xl? Ml[yNE4ydU1? MXq2bC=> NE[4W|NmdmijbnPld{B{cXK2aX7vcE1qdmS3Y3XkJIFxd3C2b4Ppdy=> M1u4dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{WxPVg6Lz5{Mke1NVk5QTxxYU6=
PC-3  M{n3SWFxd3C2b4Ppd{BCe3OjeR?= NV6zb|hsOm2P NUHPeVVmOmh? NFrWN2ZqdmO{ZXHz[ZMhV1KLLXnu[JVk\WRiY3XscEBl\WG2aB?= NIS5OGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0OVU5OCd-MkK3OFU2QDB:L3G+
HCT116 NXXnepBTSXCxcITvd4l{KEG|c3H5 MUO1cW0> MVKyOIg> M{LoV2ROW09? NVH5PFBk\W6qYX7j[ZMh[XCrZ3XubY4ucW6mdXPl[EBk\WyuIHTlZZRp M4jteFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK2OVIzLz5{NE[yOlUzOjxxYU6=
U2OS MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnS5NVBuVQ>? M1HaT|I1cA>? MlPkbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgSI95 M1v6WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkO5NFE{Lz5{NE[zPVAyOzxxYU6=
A2780cp NIT4N|lCeG:ydH;zbZMhSXO|YYm= NFTDR3IzNjWvTR?= NEjaWo4ycA>? MXXk[GgzVw>? MkDh[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> M4fHUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG3PVQ3Lz5{NEixO|k1PjxxYU6=
HepG2 MV;GeY5kfGmxbjDBd5NigQ>? MmfIOY1O NFzQVm81cA>? MWfpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? M1TlelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{GzOVg4Lz5{NEexN|U5PzxxYU6=
Microglia  M2i1[WFxd3C2b4Ppd{BCe3OjeR?= NF7Ce5E2dU1? MkfTNlRp NWXUO4xZ\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp MoLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUi2NFEoRjJ2OEG4OlAyRC:jPh?=
MDA-MB 231 NWWyUXZ1SXCxcITvd4l{KEG|c3H5 MmnMOY1O M37mSVAvPWh? MkeycY9lfWyjdHXzJHRw[2:vaX9CsuKhcW6mdXPl[EBieG:ydH;zbZM> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|MEe4NUc,OjR6M{C3PFE9N2F-
PANC-1  M4\XemFxd3C2b4Ppd{BCe3OjeR?= MnvWNY1O Mn3SOFhp M1nsXmROW09? MUDlcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> NWXtOWQ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OFIyPThpPkK0PFQzOTV6PD;hQi=>
MDA-MB-231 MnrFSpVv[3Srb36gRZN{[Xl? MmXKNo1O NIPIPHc1QGh? NI\DWYpxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? NHjmSVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O|A3PzZ:L3G+
MDA-MB-231 MULGeY5kfGmxbjDBd5NigQ>? NHTSUYczdU1? M3;DZ|I1cA>? MU\pcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi M3[0TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MCF-7  MkTLSpVv[3Srb36gRZN{[Xl? NX\qPZpxOm2P MmHLOFhp NITJXXJxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? NIPMb5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O|A3PzZ:L3G+
MCF-7  MkjuSpVv[3Srb36gRZN{[Xl? MX:ycW0> M4LHZVI1cA>? MVXpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NVXBSmw5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
HepG2 MULBdI9xfG:|aYOgRZN{[Xl? MoK4N41O MXi1bC=> M{\yZZJm\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDRSJM> NHjpXoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizOlU6PSd-MkK4N|Y2QTV:L3G+
HeLa NYTzUWR6SXCxcITvd4l{KEG|c3H5 NWLSeHBnOTCvTR?= MnrONog> MX3k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ MmroQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMECxN|UoRjJ|MECwNVM2RC:jPh?=
SK-HEP-1 NYToNnFiSXCxcITvd4l{KEG|c3H5 M3ntXlExdU1? MV:xbC=> NE\xU4lxem:2ZXP0d{Bi\2GrboP0JIF2fG:yaHHnfUBidmRiaX7keYNmeyCjcH;weI9{cXNiaX6gZpVn[Wyrbj30doVifGWmIHPlcIx{ MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh3OE[0PUc,OjJ6NUi2OFk9N2F-
MDA-MB231 MYrGeY5kfGmxbjDBd5NigQ>? M{TJb|VuVQ>? MUmxbC=> NITK[GpqdmO{ZXHz[ZMhemW|dnXyZZRzd2xvbXXkbYF1\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp M2e0WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEi4PFUxLz5{M{C4PFg2ODxxYU6=
PaCa44 MVnBdI9xfG:|aYOgRZN{[Xl? NHnDOYkzNjWvTR?= Ml7SNYg> MkjBdoVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF{NEGxNkc,OjNzMkSxNVI9N2F-
T-47D MUDGeY5kfGmxbjDBd5NigQ>? MXyxNI1O M{XlW|Jp MX\pcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? M{LQPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{CwNFI3Lz5{M{OwNFAzPjxxYU6=
GTL-16  MkjURZBweHSxc3nzJGF{e2G7 M{j3VFVuVQ>? NHS3VHUzPGh? MonBdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= M164UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{GzOFkxLz5{M{OxN|Q6ODxxYU6=
U251MG  M3T4PWZ2dmO2aX;uJGF{e2G7 NVWxSHpXO22P MU[xbC=> NWPiNlE1e3WycILld5NmeyCOQ{OtTWkheHKxdHXpckBmgHC{ZYPzbY9v NYnmc5N1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzN|g3OThpPkKzN|M5PjF6PD;hQi=>
T24 NI\Xe4VHfW6ldHnvckBCe3OjeR?= NGTPUGsyOG2P MlHsNYg> NUG1[YhRemWmdXPld{B1cGViY3zlZZZi\2Vib3[gUGM{KGGodHXyJIJicWOjbHnuJJRz\WG2bXXueC=> NH\WdHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1OFA5OCd-MkOzOVQxQDB:L3G+
HUVECs M33PUmZ2dmO2aX;uJGF{e2G7 M4n4NVNuVQ>? M1zmNFI1cA>? NUHadVNp[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= NHm3V2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1PFkzQCd-MkOzOVg6Ojh:L3G+
MCF-7  MnPDSpVv[3Srb36gRZN{[Xl? NXzVT3JYPW2P M3nM[lI1cA>? NGexb2lqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 MkOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OUW2O|koRjJ|M{m1Olc6RC:jPh?=
Hela  MoraSpVv[3Srb36gRZN{[Xl? NYqzSmN[PW2P MYWyOIg> NFPaR3ZqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NU[3PUc,OjN|OUW2O|k9N2F-
OR6 NEj4[I5HfW6ldHnvckBCe3OjeR?= NX7abmZEOTCvTR?= MXW3Nog> NETYW|l{fXCycnXzd4V{KEiFVjDy[ZBtcWOjdHnvckBidmRiZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NUi3OUc,OjN|OUW4O|U9N2F-
HT-29  M1HuNmZ2dmO2aX;uJGF{e2G7 NG\6U|AydU1? MUS0PE86Pmh? M1vCWolvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= NGDY[lg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WwPFI4Oid-MkO1NFgzPzJ:L3G+
SH-SY5Y NVPieXhWS3m2b4TvfIlkcXS7IFHzd4F6 M{LT[lVuVQ>? MYiyOIg> MWHpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 M4i2TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK1NlY2Lz5{M{WyOVI3PTxxYU6=
Saos-2  MV3BdI9xfG:|aYOgRZN{[Xl? NV;kNY54OW2P NXzIdWJsQT[q MWLpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KFCFWB?= NUHrXmltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OlMyPzFpPkKzOVY{OTdzPD;hQi=>
1321N1 NH:2Z2tEgXSxdH;4bYNqfHliQYPzZZk> NHXnTWc2dU1? NVHTW|RvOjSq MWDwdo91\WO2czDj[YxtKGGpYXnud5QhWEOQLXnu[JVk\WRidH;4bYNqfHl? MoDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkWyOlUoRjJ|NUK1NlY2RC:jPh?=
A2780 M1vwcGFxd3C2b4Ppd{BCe3OjeR?= NFzPT2Y2dU1? Mk\1NlRp MVrjc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? M3HhUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUmyNlgyLz5{M{W5NlI5OTxxYU6=
OV2008 NXjS[IxuSXCxcITvd4l{KEG|c3H5 MVq1cW0> MnK0NlRp NGrHbXBkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7MkK4NUc,OjN3OUKyPFE9N2F-
PC12  NH;i[oNHfW6ldHnvckBCe3OjeR?= M3m0TVExdU1? M2[zUFI1cA>? NGLwb3J4[XSnch?= NE\CdZrDqGmwaHnibZR{KGOqeX3veJJ6eHOrbj3sbYtmKHC{b4TlZZNwdWGuIHHjeIl3cXS7Lh?= MleyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEO5O|koRjJ|NkCzPVc6RC:jPh?=
SH-SY5Y M4LrWWFxd3C2b4Ppd{BCe3OjeR?= Ml;YOY1O NVHQNnRJOWh? NIXFc4Ji[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOUO5OUc,OjN4MUmzPVU9N2F-
SH-SY5Y M2nBd2Z2dmO2aX;uJGF{e2G7 NUHWOXVmOW2P Mlr3NlRp MVLpcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF;DVC=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2M{G0PEc,OjN5NEOxOFg9N2F-
HepG2 MXXGeY5kfGmxbjDBd5NigQ>? M3v3eVExdU1? Mm\YNlRp MXLpcohq[mm2czDzbXRKT0GULTDhcoQhUEKVUz3pcoR2[2WmIHH1eI9xcGGpeR?= MmKwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MUewOFAoRjJ|OEG3NFQxRC:jPh?=
HeLa NIrLXWRHfW6ldHnvckBCe3OjeR?= MlzCNVBuVQ>? M3LzcFJp M3foS5N2eHC{ZYPz[ZMhVEN|IFnJJIV5eHKnc4Ppd49v NXLXNnh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlQ4OzhpPkKzPFY1PzN6PD;hQi=>
HONE-1  MXPGeY5kfGmxbjDBd5NigQ>? MmrUOY1O Ml32NYg> MVPy[ZBz\XO|ZYOgOpIudWWmaXH0[YQhWk:VIIDyc4R2[3Srb36= M1rESlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEmyN|U5Lz5{M{i5NlM2QDxxYU6=
MCF7 MVTGeY5kfGmxbjDBd5NigQ>? NFu1XHc2dU1? M1nMS|I1cA>? MV3pcoNz\WG|ZYOgR5VQKGmwZIXj[YQh[2WubDDk[YF1cA>? NVT5XFA3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OlI3OjlpPkKzPVYzPjJ7PD;hQi=>
HO8910 NVy0Wo1mSXCxcITvd4l{KEG|c3H5 MkPZNVBuVQ>? MVixNog> MnnY[Y5p[W6lZYOgRlE6NWmwZIXj[YQh[XCxcITvd4k> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6M{[xNEc,OjN7OEO2NVA9N2F-
SMMC-7721 MUPBdI9xfG:|aYOgRZN{[Xl? MWW1cW0> Mmr4NlRp NWPCb4Zu[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ MlT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNk[2PVMoRjJ2ME[2Olk{RC:jPh?=
Hep3B NUm4O5JQSXCxcITvd4l{KEG|c3H5 MV61cW0> MWGyOIg> MUjheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= NYXw[VNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl|PD;hQi=>
H460 NImx[ndHfW6ldHnvckBCe3OjeR?= NGn2OoQyOG2P NYHve3Y1PGh? NHnNWnpqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NHSyfVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3N|IxQCd-MkSxO|MzODh:L3G+
A549 M3HC[GZ2dmO2aX;uJGF{e2G7 NVHvWXBmOTCvTR?= NILlbFY1cA>? M3rCSYlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w NVzkNVQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFI4OzVpPkK0NVQzPzN3PD;hQi=>
H1299  MnLpSpVv[3Srb36gRZN{[Xl? NU\FUGVVOTCvTR?= NVTLXoJKPGh? M3L1R4lv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NYX5W5lqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|MzODhpPkK0NVc{OjB6PD;hQi=>
WiDr NFLEOnlHfW6ldHnvckBCe3OjeR?= NUjjcW0{OTCvTR?= MmDrNYg> MYDpcohq[mm2czDQR2JNNWmwZIXj[YQhVEN|IFnJJIV5eHKnc4Ppc44> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF7MES4PUc,OjRzOUC0PFk9N2F-
LoVo  NUG4XYJoSXCxcITvd4l{KEG|c3H5 MoixOY1O MlKxOFhp NVzJRZI6\W6qYX7j[ZMhTEODLXnu[JVk\WRiYYDvdJRwe2m|Lh?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJyMUixNkc,OjR{MEG4NVI9N2F-
HepG2 E47 MUXGeY5kfGmxbjDBd5NigQ>? MkTqNk42dU1? M{fxZ|Q5cA>? MX\pcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 M4Ln[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkezO|M5Lz5{NEK3N|c{QDxxYU6=
RKO M{\OeGZ2dmO2aX;uJGF{e2G7 NYTMUFhVOm2P NXnvZllpOWh? MUjEUXNQ M{HuNIVvcGGwY3XzJINmdGxiZHXheIgh[nliZ3Xs[IFv[W27Y3nu MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7MUe3O{c,OjR{OUG3O|c9N2F-
Hep3B MV7BdI9xfG:|aYOgRZN{[Xl? MVKycW0> MX2xNog> NX3LUYs1TE2VTx?= NHTHfYpqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> M4C1OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
ACHN-5968 M1HCU2Fxd3C2b4Ppd{BCe3OjeR?= MoLxOY1O M{TlNVNp Mo\p[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? M3fXWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{C1OlA1Lz5{NEOwOVYxPDxxYU6=
Huh7 NYm4TJZLSXCxcITvd4l{KEG|c3H5 NIHpVW0zdU1? M{DlfVEzcA>? NV\HcmFvTE2VTx?= NGXaSG9qdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7N{OwNEc,OjR{OUezNFA9N2F-
UOK257 M1[1VWFxd3C2b4Ppd{BCe3OjeR?= M13wRVVuVQ>? MVyzbC=> NIP4PVZmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NIrEOWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
ECSCs  NITEU5lCeG:ydH;zbZMhSXO|YYm= NI[4[G0yOG2P NGjjR|U1cA>? MorQ[IVkemWjc3XzJJJieGGveXPpck11emWjdHXkJIFxd3C2b4Ppdy=> NXXQdYtMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVkyODlpPkK0N|E6OTB7PD;hQi=>
MCF-7  NGjPWXBHfW6ldHnvckBCe3OjeR?= NYLnNXY6OTCvTR?= MX2yOIg> NVyydZJ{cW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JINp\W2xdHjldoFxgSCmcoXndy=> NGjrRYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOxOVU4QCd-MkSzNVU2Pzh:L3G+
SGC-7901  NXv5PVdnSXCxcITvd4l{KEG|c3H5 NUKyd5Y1Om2P NGjG[JoycA>? MmnpbY5kemWjc3XzJGNCNTRiaX7keYNm\CCjcH;weI9{cXN? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{MUO0NEc,OjR|MkGzOFA9N2F-
SMMC-7721 M4WxcWFxd3C2b4Ppd{BCe3OjeR?= MnTaNo1O M3u2VFFp M1XifYlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| MnH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkGzOFAoRjJ2M{KxN|QxRC:jPh?=
T24 MlTRRZBweHSxc3nzJGF{e2G7 MlznOY1O MYWxMlVp MVzwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> NHfBV209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0PVE4Pid-MkSzOFkyPzZ:L3G+
NTUB1 M2TzV2Fxd3C2b4Ppd{BCe3OjeR?= MXu1cW0> MYOxMlVp NELNVXNxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? NYHBcJgzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N|Q6OTd4PD;hQi=>
MG-63 MXjBdI9xfG:|aYOgRZN{[Xl? NYPMOZV7OTCvTR?= M1HUfVEzcA>? NVjVbGxY\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? M13iR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|AyLz5{NEO1PFMxOTxxYU6=
MG-63 NHK0RlhCeG:ydH;zbZMhSXO|YYm= MnHONE42NzGvTR?= MXKzNog> MYflcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| NG\EUZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
MG-63 NGPF[HRHfW6ldHnvckBCe3OjeR?= MUGwMlUwOW2P NXPob3dPPDiq M{PJTIlv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ M2LlbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|QzLz5{NEO1PFM1OjxxYU6=
U2OS  MnnqSpVv[3Srb36gRZN{[Xl? MV:wMlUwOW2P M3\TUVQ5cA>? MWHpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> NEXQTWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
HGC-27 M2jw[GZ2dmO2aX;uJGF{e2G7 MWCxNI1O MXixbC=> M{fXS4lvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 NV7qd4JDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NVY{PDlpPkK0OFE3OzR7PD;hQi=>
HCT116  NGXEUJBCeG:ydH;zbZMhSXO|YYm= MVG1cW0> NVfTOnZxOjSq M{frV4VvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= MkP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
A549 M2\Rb2Fxd3C2b4Ppd{BCe3OjeR?= MUCxNI1O NUPxfWpIPDiq Mnj0ZYNk\WyncnH0[ZMhfGinIILl[JVkfGmxbjDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JHBVYA>? M3rvNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK2O|IzLz5{NE[yOlczOjxxYU6=
Saos-2 NFWybINCeG:ydH;zbZMhSXO|YYm= MoC0NVBuVQ>? M1nP[VI1cA>? M4T5[4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OUCxN{c,OjR4M{mwNVM9N2F-
U2OS NG\QVoVCeG:ydH;zbZMhSXO|YYm= MUOxNI1O MofBNlRp MUHpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> NXPmeGxXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
HepG2 MlXzSpVv[3Srb36gRZN{[Xl? MUG1cW0> NEL3PGg1cA>? NHXPNm9qdmO{ZXHz[ZMhUExicnXs[YF{\Q>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzM{W4O{c,OjR5MUO1PFc9N2F-
A549 MWnBdI9xfG:|aYOgRZN{[Xl? NFPuUIM2dU1? M4fkblQ5cA>? MWjk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdCCmZXH0bEBidmRiZYjwdoV{e2mxbjDs[ZZmdHNib3[gZ4F{eGG|ZT2zMEBD\WOuaX6tNUBidmRiTFOzMWlK MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdyNkOwN{c,OjR5ME[zNFM9N2F-
A2780cp MXTBdI9xfG:|aYOgRZN{[Xl? MVGyMlVuVQ>? NETRWIgycA>? M1[zVoRlUDKR M4\3e4VvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MnvqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUe5OFYoRjJ2OEG3PVQ3RC:jPh?=
Microglia  MoW4RZBweHSxc3nzJGF{e2G7 NXn2[WVuPW2P M2TJU|I1cA>? M{HnRYRm[3KnYYPld{BpgXCxeHnhMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NGPxbHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixPFYxOSd-MkS4NVg3ODF:L3G+
HT-29  MmrvRZBweHSxc3nzJGF{e2G7 M3LFclFuVQ>? MojYOFhp M{GxeWROW09? NHnlTWRmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? M{Hnd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OESyNVU5Lz5{NEi0NlE2QDxxYU6=
MDR M1Kzd2Fxd3C2b4Ppd{BCe3OjeR?= NFHoPYQyOG2P Mmm5Oog> MVTzeJJmdme2aHXud{B1cGVicH;3[ZIhd2ZiYX70bYNidmOncjDkdpVoew>? M33VOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEG5O|AyLz5{NUCxPVcxOTxxYU6=
H157 Mmm4SpVv[3Srb36gRZN{[Xl? MYq1cW0> NWLjWGE{Omh? NYrNd2ZEe3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK NFjldGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK4OVYzQCd-MkWyPFU3Ojh:L3G+
A549  MWLGeY5kfGmxbjDBd5NigQ>? MnPvOY1O Ml;GNog> MWXzeZBxemW|c3XzJHNRSyCrbnT1Z4VlKGGlY4XteYxifGmxbjDv[kBNSzNvSVm= NH21NVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK4OVYzQCd-MkWyPFU3Ojh:L3G+
A2780cp  NIDqOYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYGxcW0> MVixbC=> M{HJfolv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN{Mk[5OEc,OjV|MkK2PVQ9N2F-
NBL-W-S  MlLYRZBweHSxc3nzJGF{e2G7 MVWxcW0> MXe2bC=> M1zjcYlv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGdCVlRvNkG= NGPN[3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOyN|IzOid-MkWzNlMzOjJ:L3G+
NBL-W-S  MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWL0Wmd3OW2P MYe2bC=> MnfP[Y5p[W6lZYOgS2FPXC14MTD0c5hq[2m2eR?= NH[1fmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOyN|IzOid-MkWzNlMzOjJ:L3G+
A549  MkTmRZBweHSxc3nzJGF{e2G7 MVu1cW0> M3\LSlJp MVvEUXNQ M1XvTIlvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= NInqT2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUexOlU3OSd-MkW3NVY2PjF:L3G+
95D NFH1SohCeG:ydH;zbZMhSXO|YYm= NWLDZodlPW2P MnvxNog> NGPNfm5FVVOR NXXFTIhicW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo M{LxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G2OVYyLz5{NUexOlU3OTxxYU6=
A549  NVn1O2VpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XaSlNuVQ>? M33IZlJp Ml6ySG1UVw>? MX7y[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E NHq5T3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUexOlU3OSd-MkW3NVY2PjF:L3G+
95D NUHCdGp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVSzcW0> MU[ybC=> NFflXFJFVVOR NUPx[5lSemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NXTGd2U6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVY2PjFpPkK1O|E3PTZzPD;hQi=>
Nara-H M1G1bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;LR|VuVQ>? NYrvOWVnPDiq NIfWOnZmdmijbnPld{B1\W2|aYLvcIlufXOvZXTpZZRm\CC|dYDwdoV{e2mxbjDv[kBP[XKjLVigZ4VtdCCycn;sbYZmemG2aX;u Mnz3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEWwN|MoRjJzOEC1NFM{RC:jPh?=
HUVECs NH;ZN4tHfW6ldHnvckBCe3OjeR?= NG\LSmsyOG2P NWTUS4hnOC53aB?= NHmzWpBl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l MmTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2Nki1PVIoRjJzNE[4OVkzRC:jPh?=
HepG2 NUHG[HRmSXCxcITvd4l{KEG|c3H5 NXXWbWpuOm2P MX:xbC=> M1HySoVvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxiZHXheIg> NXXyU5FPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OVM3QTFpPkKxOFU{PjlzPD;hQi=>
U-2 OS M1XaU5FJXFNiYYPzZZk> MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| NV;3ZVJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673 MWDxTHRUKGG|c3H5 MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NE\xU209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY M3;BW5FJXFNiYYPzZZk> NVfFfYF7eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= NWX0[FVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NUHzVHk6eUiWUzDhd5NigQ>? NXv6bY9WeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-37 MlOzdWhVWyCjc4PhfS=> MWLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? NUTpV3lRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD M3LYSpFJXFNiYYPzZZk> NHuwTnhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUlSgZ4VtdHN? M3vlflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH M{XCOZFJXFNiYYPzZZk> M3\STZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 MWHxTHRUKGG|c3H5 MYjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvMUKgZ4VtdHN? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) NUC0WJZreUiWUzDhd5NigQ>? NEPwTVVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 NYLud5JzeUiWUzDhd5NigQ>? MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M{jBelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MoDpdWhVWyCjc4PhfS=> NWi3UotseUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF;IV{02OCClZXzsdy=> Mnf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 NYO0cIRleUiWUzDhd5NigQ>? NFHic2hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= MmPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 NUjMe3k5eUiWUzDhd5NigQ>? NVvPPGNSeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoN|Ah[2WubIO= MoLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 NYW4XYRGeUiWUzDhd5NigQ>? MofFdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC NUjkSW5UeUiWUzDhd5NigQ>? MXjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 MmLLdWhVWyCjc4PhfS=> MmXFdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NYe3cYJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 M4P1V5FJXFNiYYPzZZk> NFXFWYNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M17L[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 MUfxTHRUKGG|c3H5 MUTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? NGjhWmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65; 

PubMed: 29296191     


Western blotting analysed expression of α-SMA, TGF-β, LC-3B, Beclin-1, NF-κB and NF-κBp65 in hepatic stellate cells.

caspase-3 / caspase-9 / PARP; 

PubMed: 25891159     


EC9706 cell lysates were prepared and subjected to immunoblotting with antibodies against caspase-3, caspase-9 and PARP. The expression ratios in each experiment were determined densitometrically. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; PI, propidum iodide; PARP, poly(ADP ribose) polymerase.

VEGF; 

PubMed: 25891159     


Effects of autophagy inhibition on the protein expression levels of VEGF. Western blot analysis demonstrated that VEGF protein expression levels were decreased when the EC9706 human esophageal squamous cell carcinoma cells were treated with 3-MA. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; VEGF, vascular endothelial growth factor.

APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2; 

PubMed: 26316755     


Presenilin 1, Nicastrin, and Pen-2 were increased with the 3-MA treatment (*P<0.001) while the other component of the γ-secretase complex (APH-1 and Presenilin 2) has no difference. 3-MA, 3-methyladenine; Con, control; APP, amyloid protein precursor.

29296191 25891159 26316755
Immunofluorescence
LC3 / Hif-α / COX2; 

PubMed: 29039446     


Chemical interventions of autophagy and ischemic-hypoxic injury, assessed using immunochemical double labeling. Compared with the control group, expression levels of HIF-α and COX2 were altered by chemical intervention. (A) Chemical intervention altered the expression of HIF-α and autophagosmes. Following autophagy antagonist (3-MA) intervention, expression of HIF-α was enhanced, compared with that in the OGD group, and was decreased by the autophagy agonist, rapamycin. In the MHY treatment group, expression of HIF-α was similar to that in the 3-MA group. (B) Chemical intervention showed changes in the expression of COX2 and autophagosomes. Changes in the expression of COX2 were similar to changes in HIF-α. Scale bar, 30 µm. OGD, oxygen-glucose deprivation; HIF-α, hypoxia-inducible factor-α; COX2, cyclooxygenase 2; 3-MA, 3-methyladenine; RP, rapamycin; MHY, MHY1485.

29039446
Growth inhibition assay
Cell viability; 

PubMed: 26934124     


KYSE 180 cells were co-treated with EDHB (40 μg/ml) and various concentrations of 3-MA for 24 h (left) or 48 h (right), and cell viability was determined by WST-8 assays.

26934124
体内研究 3-Methyladenine (3-MA)可以通过对磷酸肌醇3磷酸激酶(PI3K) 的作用来阻断自吞噬, 而PI3K的活性对于自噬体形成早期膜池的成核和组装是必须的。与SAH 处理组相比3-MA并不会改变出血的程度。与SAH +对照成分组相比经过3-MA预处理后会明显加重神经病学症状。3-MA处理会减少自吞噬发生[5]。 相反地,在SAH + 3-MA组里 断裂的半胱天冬酶表达量明显上调，与此一致的是与SAH + 对照成分组相比，SAH + 3-MA组中右脑皮层里原位末端标记阳性细胞数量明显增多[5]。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验：[4]
- 合并

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
细胞实验：[2]
- 合并
  • Cell lines: HeLa 细胞系
  • Concentrations: 1-10 mM
  • Incubation Time: 3 分钟
  • Method: 用台盼蓝染色法分析细胞存活率（如HeLa细胞）。简言之, 经过3-MA处理后将贴壁的与漂浮的细胞都用PBS( pH 7.4)重悬，密度控制在每毫升1-2×106。加入等体积的0.4% 台盼蓝并彻底混匀。室温孵育3分钟后用血细胞计数器对细胞计数分析。
    (Only for Reference)
动物实验：[5]
- 合并
  • Animal Models: 体重 300-350 g 的成年雄性Sprague–Dawley 大鼠
  • Dosages: 400 nM
  • Administration: 脑室给药
    (Only for Reference)

溶解度 (25°C)

体外 Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 149.15
化学式

C6H7N5
CAS号 5142-23-4
储存条件 粉状
溶于溶剂
别名 NSC 66389

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量 只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量：

总分子量：g/mol

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1：

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • 回答：

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

Selleck产品目录册

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

相关PI3K产品

  • Taselisib (GDC 0032) New

    Taselisib (GDC 0032, RG7604) 是一种有效，下一代的β亚型PI3K抑制剂，作用于PI3Kα/δ/γ， IC50分别为0.29 nM/0.12 nM/0.97nM，比作用于PI3Kβ选择性高10倍以上。

  • Pilaralisib (XL147) New

    Pilaralisib (XL147)是一种选择性的可逆I型PI3K抑制剂，作用于PI3Kα/δ/γ，在无细胞试验中IC50为 39 nM/36 nM/23 nM，对PI3Kβ作用较低。Phase 1/2。

  • GNE-317 New

    GNE-317 是一种有效的，大脑渗透性 PI3K 抑制剂。

  • LY294002

    LY294002 (SF 1101, NSC 697286) 是首个合成的已知抑制PI3Kα/δ/β的小分子，在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM；在溶液中比在Wortmannin中稳定，也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶，还能够结合一些与PI3K家族无关的新型靶点。LY294002 可以抑制CK2，对应的IC50值为98 nM。LY294002 是一种非特异的 DNA-PKcs 抑制剂，并可激活自噬和凋亡。

    Features:LY294002是选择性的, 可渗透细胞的特定PI3K抑制剂，可作用于酶的ATP结合位点，且抑制自体吞噬的螯合作用。

  • Idelalisib (CAL-101, GS-1101)

    Idelalisib (CAL-101, GS-1101) 是选择性p110δ抑制剂，在无细胞试验中IC50为 2.5 nM；对 p110δ 表现出的选择性是对 p110α/β/γ 的 40 到 300 倍，对p110δ的选择性是对 C2β，hVPS34，DNA-PK 和 mTOR的400到4000倍。Idelalisib 还可诱导自噬。

    Features:Calistoga 暗示 CAL-101治疗血液恶性肿瘤可能有更广泛的应用价值。

  • Wortmannin

    Wortmannin (KY 12420)是一种首次命名的PI3K抑制剂，在无细胞试验中IC50为3 nM，对 PI3K 家族的选择性较低。Wortmannin 可抑制自噬体的形成，并有效抑制DNA-PK/ATM，在无细胞试验中IC50为16 nM和150 nM。Wortmannin 也能抑制 PLK1 的活性。

  • Dactolisib (BEZ235)

    Dactolisib (BEZ235, NVP-BEZ235) 是一种双重ATP竞争性 PI3K 和 mTOR 抑制剂，在无细胞试验中，抑制 p110α/γ/δ/β 和 mTOR(p70S6K) 的 IC50 分别为 4 nM /5 nM /7 nM /75 nM /6 nM。 在 3T3TopBP1-ER 细胞中抑制 ATR，IC50 为 21 nM，而对 Akt 和 PDK1 的抑制作用很弱。Dactolisib可诱导自噬并抑制HIV-1的复制。Phase 2。

  • Buparlisib (BKM120)

    Buparlisib (BKM120, NVP-BKM120) 是一种选择性 PI3K 抑制剂，在无细胞试验中作用于p110α/β/δ/γ的IC50分别为 52 nM/166 nM/116 nM/262 nM。对 VPS34，mTOR，DNAPK 的作用效果下降，而对 PI4Kβ几乎没有活性。Buparlisib 可诱导凋亡。Phase 2。

    Features:BKM120是可用于口服的生物有效的PI3K抑制剂。

  • Pictilisib (GDC-0941)

    Pictilisib (GDC-0941, RG7321) 是一种有效的PI3Kα/δ抑制剂，在无细胞试验中IC50为 3 nM，对p110β (11倍)和p110γ (25倍)具有适度的选择性。Pictilisib (GDC-0941) 可诱导自噬和凋亡。Phase 2。

  • Alpelisib (BYL719)

    Alpelisib (BYL719) 是一种有效的选择性PI3Kα抑制剂，在无细胞试验中IC50为 5 nM，对PI3Kβ/γ/δ具有极弱的作用。Phase 2。

Tags: 购买3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)供应商 | 采购3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)价格 | 3-Methyladenine (3-MA)生产 | 订购3-Methyladenine (3-MA) | 3-Methyladenine (3-MA)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID