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3-Methyladenine (3-MA)

目录号：S2767 别名: NSC 66389 中文名称：3-甲基腺嘌呤

仅限科研使用

3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，在 HeLa细胞中IC50分别为25 μM和60 μM；永久性抑制I型PI3K，但对III型PI3K的抑制是短暂的，也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用（加热助溶）。

3-Methyladenine (3-MA) Chemical Structure

CAS: 5142-23-4

规格	价格	库存
50mg	RMB 579.66	现货
500mg	RMB 3010.37	现货
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客户使用Selleck生产的3-Methyladenine (3-MA)发表文献693篇：

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述

靶点

Vps34 ^[1] (HeLa cells)	PI3Kγ ^[1] (HeLa cells)
25 μM	60 μM

体外研究

3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的^[1] 。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵，这表明3-MA 除了抑制自吞噬之外还有其它生物功能。3-MA可以造成半胱天冬酶依赖的细胞死亡，这一功能与它对自吞噬的抑制无关。用5mM 3-MA处理葡萄糖饥饿Hela细胞可降低gfp-lc3阳性细胞比例至23%。3-MA处理细胞12到48小时内 LC3-I 的水平上升而LC3-II 水平下降。LC3-I转为为 LC3-II 的过程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 处理HeLa 细胞一天并不影响细胞存活率, 而 10 mM 3-MA 处理一天会造成细胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 处理细胞两天分别使细胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低细胞存活率的作用具有时间和剂量依赖特性。3-MA 明显缩短nocodazole诱导的前中期阻断时间^[2]。3-MA 通过抑制自吞噬来抑制SU11274诱导的细胞死亡^[3]。在野生型MEF细胞中延长 3-MA处理时间 (最多 9 小时) 明显造成 LC3 I 到 II 转换。延长 3-MA处理时间会明显增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介导的LC3 转换和游离 GFP 释放是ATG7依赖的。 3-MA处理会导致 p62 蛋白水平明显升高。即使在Atg5−/− 的MEF细胞中3-MA 也会使p62 水平升高，就像在DOX介导的ATG5缺失的细胞中一样。3-MA以不同方式抑制 I 型和 III型 PI3K。与野生型细胞相比，在Tsc2−/−细胞中3-MA诱导的 LC3 I 到 LC3 II 转换过程大幅下降。3-MA会破坏mTOR复合物1的拮抗自吞噬功能^[4]。

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

K562	NFfudoZHfW6ldHnvckBCe3OjeR?=	NF;nfI0yOG2P	NYGwVmhTOWh?		M2jIUIRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO=	M2HpS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
Jurkat	MV7GeY5kfGmxbjDBd5NigQ>?	NUDjPJc1OTCvTR?=	MoexNYg>		M4L1T4Rm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO=	M3G2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
HeLa	Moj5R5l1d3SxeHnjbZR6KEG\|c3H5	MXqycW0>	NInYcYEzPGh?		NHTI[FJqdmirYnn0[ZMhfGinIHP5eI91d3irY3n0fUBw\iC\|aXzpZolvcW5idH:gTIVN[SClZXzsd{4>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5NUO4OUc,OjF6N{WzPFU9N2F-
PC12/TetOn	M3ey[GZ2dmO2aX;uJGF{e2G7	NGP6[HkxNjFxMX3N	NXnTcVFIOTiq		NUDzTYhidGWjZIOgeI8h\|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC=	NIjRZZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwOlY2QSd-MkG5NFY3PTl:L3G+
RMPI8226	MUTGeY5kfGmxbjDBd5NigQ>?	MmrDOY1O	M17Hb\|Fp		NYD2WJZje3WycILld5NmeyC2aHWgcIV3\Wxib3[gZZV1d3CqYXf5JJVv\GW{IH71eJJq\W62IHTldIxmfGmxbh?=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlzNU[yNEc,OjF7MUW2NlA9N2F-
MCF-7	NGrwdGJHfW6ldHnvckBCe3OjeR?=	MmfoNVBuVQ>?	NIfoPGs1QGh?		Mn\oZoxw[2u\|IHH1eI9xcGGpeTDpcoR2[2WmIHL5JIJwenSnen;tbYI>	MlfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7M{G5N\|coRjJzOUOxPVM4RC:jPh?=
HBx	NIL1THRCeG:ydH;zbZMhSXO\|YYm=	NHfsRngyOG2P	MYG0PIg>	MXfEUXNQ	MnXXbY5kemWjc3XzJINmdGxiZHXheIg>	Mn;nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkCwO\|goRjJ{MEKwNFc5RC:jPh?=
Marc-145	MVjGeY5kfGmxbjDBd5NigQ>?	NWXifWVwPW2P	NE\ZWoEyOi9{ND:zOog>		MmrWdoVlfWOnczD0bIUhWFKUU2[geIl1\XK\|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937	NXzB[FhsTnWwY4Tpc44hSXO\|YYm=	MlXWNo1O	MnrDNVJp		M3ruPYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB?	MoD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNUWxOVAoRjJ{MUW1NVUxRC:jPh?=
BGC-823	M1zqNWZ2dmO2aX;uJGF{e2G7	M1e4VFVuVQ>?	NGX5[\|IzcA>?		NYPjbWNLcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>?	NG\6OWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNlE2Oid-MkKzNlIyPTJ:L3G+
A549	MVXGeY5kfGmxbjDBd5NigQ>?	NIrxVHAxNjGvTR?=	M3TobFI1cA>?		M37nTZN2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4Nkm2NEc,OjJ2Nk[5OlA9N2F-
pDCs	M4jnRWZ2dmO2aX;uJGF{e2G7	NUjubYVCOTCvTR?=	MnX6NE42cA>?		NFPRcHnDqHKnZIXj[ZMhfGinIHnu[JVkfGmxbjDv[kBKTk5vzsGgZpkhe3OUTlG0NC=>	M4TYVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{m2OVk6Lz5{MkO5OlU6QTxxYU6=
HeLa	NFPKfHhCeG:ydH;zbZMhSXO\|YYm=	MmHqOY1O	NYHxdmdXOjSq		MknQbY5lfWOnczDjZZNx[XOnLXTldIVv\GWwdDDj[YxtKGSnYYTo	NVPTb3dNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFUyOjhpPkKyOVQ2OTJ6PD;hQi=>
U251	NIq2T5BCeG:ydH;zbZMhSXO\|YYm=	NIjQWpU2dU1?	M130NVI1cA>?		M165V4lv[3KnYYPld{BUOS2rbnT1Z4VlKGOnbHyg[IVifGh?	NYn5cYJmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1O\|k4QDhpPkKyOVc6Pzh6PD;hQi=>
MCF-7	Ml7zRZBweHSxc3nzJGF{e2G7	NXi2NoJOOC5zbV2=	M1;hWlZp		NULONXdn\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM>	MmXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NUG5PFkoRjJ{N{WxPVg6RC:jPh?=
PC-3	NHTXRpJCeG:ydH;zbZMhSXO\|YYm=	NILpNmwzdU1?	NVPpVWY6Omh?		NWm0dYNWcW6lcnXhd4V{KE:UST3pcoR2[2WmIHPlcIwh\GWjdHi=	NHX3cJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0OVU5OCd-MkK3OFU2QDB:L3G+
HCT116	NHWzWY1CeG:ydH;zbZMhSXO\|YYm=	NUSxSoVDPW2P	NGjWXoMzPGh?	NHz0WmJFVVOR	NUjlNGd2\W6qYX7j[ZMh[XCrZ3XubY4ucW6mdXPl[EBk\WyuIHTlZZRp	MljLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
U2OS	NH\hT25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4DRe\|ExdU1?	MWWyOIg>		MXnpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g>	NWrvT2tSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|kxOTNpPkK0OlM6ODF\|PD;hQi=>
A2780cp	NU\LOFY3SXCxcITvd4l{KEG\|c3H5	MkfiNk42dU1?	MmT1NYg>	MUjk[GgzVw>?	NX;sXZpu\W6qYX7j[ZMh[2m\|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>?	M2PrT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG3PVQ3Lz5{NEixO\|k1PjxxYU6=
HepG2	M3LjcWZ2dmO2aX;uJGF{e2G7	MYS1cW0>	M{HycFRp		M{T6UYlv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?=	NX\5[mYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O\|E{PTh5PD;hQi=>
Microglia	Mn\MRZBweHSxc3nzJGF{e2G7	M3jZclVuVQ>?	NFLFWWMzPGh?		MWXk[YNz\WG\|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi=	M2rCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG4OlAyLz5{NEixPFYxOTxxYU6=
MDA-MB 231	Mnm1RZBweHSxc3nzJGF{e2G7	MkTlOY1O	MnjXNE42cA>?		NITuO4Zud2S3bHH0[ZMhXG:lb33pcuKvyqCrbnT1Z4VlKGGyb4D0c5Nqew>?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh\|MEe4NUc,OjR6M{C3PFE9N2F-
PANC-1	NIi5TYpCeG:ydH;zbZMhSXO\|YYm=	MYixcW0>	Mly0OFhp	Mo\qSG1UVw>?	NYK1VFVm\W6qYX7j[ZMh[m:{dHX6c41q[i2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N?	NWLKZmFkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OFIyPThpPkK0PFQzOTV6PD;hQi=>
MDA-MB-231	M2\6SmZ2dmO2aX;uJGF{e2G7	NXztVY5WOm2P	MlvwOFhp		NEXCWWxxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh?	NIfl[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O\|A3PzZ:L3G+
MDA-MB-231	MkLTSpVv[3Srb36gRZN{[Xl?	M{fZZ\|JuVQ>?	NH35cIYzPGh?		MYHpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi	NEnjOGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O\|A3PzZ:L3G+
MCF-7	M{DNOmZ2dmO2aX;uJGF{e2G7	MVWycW0>	M3WyWVQ5cA>?		M170ZpBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>?	MlTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7N{C2O\|YoRjJ2OUewOlc3RC:jPh?=
MCF-7	NGnRUpBHfW6ldHnvckBCe3OjeR?=	NXX1b5VzOm2P	NH;PbWgzPGh?		M4XqSYlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5ME[3Okc,OjR7N{C2O\|Y9N2F-
HepG2	MmHKRZBweHSxc3nzJGF{e2G7	MmDrN41O	MWS1bC=>		NFjFVlJz\WS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnu[JVk\WRiYomgVWR{	NHG1d\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizOlU6PSd-MkK4N\|Y2QTV:L3G+
HeLa	NFLlWIlCeG:ydH;zbZMhSXO\|YYm=	NXjvS4Q4OTCvTR?=	MYSybC=>		NHfSWXdl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK	M13CdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MECwNVM2Lz5{M{CwNFE{PTxxYU6=
SK-HEP-1	M{XkWWFxd3C2b4Ppd{BCe3OjeR?=	MnfhNVBuVQ>?	MU[xbC=>		MYLwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz	NXTmdndkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OVg3PDlpPkKyPFU5PjR7PD;hQi=>
MDA-MB231	NH7jPYVHfW6ldHnvckBCe3OjeR?=	MmfYOY1O	MY[xbC=>		MV;pcoNz\WG\|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G\|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo	MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyOEi4OVAoRjJ\|MEi4PFUxRC:jPh?=
PaCa44	M33hNGFxd3C2b4Ppd{BCe3OjeR?=	M{PaWlIvPW2P	Ml3yNYg>		NHqwdo9z\WS3Y3XzxsBo\W6rcHnuMY1m\GmjdHXkJIFxd3C2b4Ppdy=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF{NEGxNkc,OjNzMkSxNVI9N2F-
T-47D	MVjGeY5kfGmxbjDBd5NigQ>?	MVexNI1O	NXvZZVdVOmh?		NY[0cIN7cW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:\|aYO=	M3HOfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{CwNFI3Lz5{M{OwNFAzPjxxYU6=
GTL-16	NFHTZY1CeG:ydH;zbZMhSXO\|YYm=	MVm1cW0>	Mlz3NlRp		M{\EUpJm\HWlZYOgZ4VtdCC4aXHibYxqfHliYYOgZ49ueGG{ZXSgeI8h[2WubIOgeJJm[XSnZDD3bZRpKE2HVDDpcohq[mm2b4Lz	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN\|MUO0PVA9N2F-
U251MG	M4jwXmZ2dmO2aX;uJGF{e2G7	MV2zcW0>	M2nJe\|Fp		NFLCeFZ{fXCycnXzd4V{KEyFMz3JTUBxem:2ZXnuJIV5eHKnc4Ppc44>	NH71UHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OzPFYyQCd-MkOzN\|g3OTh:L3G+
T24	MUfGeY5kfGmxbjDBd5NigQ>?	MmriNVBuVQ>?	M{frO\|Fp		MmPydoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3NEC4NEc,OjN\|NUSwPFA9N2F-
HUVECs	NUnkUFBoTnWwY4Tpc44hSXO\|YYm=	M1fT[\|NuVQ>?	M3fRNFI1cA>?		MYHicI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6	NIrlRXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1PFkzQCd-MkOzOVg6Ojh:L3G+
MCF-7	MWLGeY5kfGmxbjDBd5NigQ>?	MXS1cW0>	Ml70NlRp		M3zHW4lvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k>	MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|OUW2O\|koRjJ\|M{m1Olc6RC:jPh?=
Hela	NWTqNIN4TnWwY4Tpc44hSXO\|YYm=	MUO1cW0>	NFGxe4QzPGh?		MnTFbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=>	NHO1fFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVY4QSd-MkOzPVU3Pzl:L3G+
OR6	MXLGeY5kfGmxbjDBd5NigQ>?	MnXCNVBuVQ>?	NEDlb484Omh?		M3\5Z5N2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NUi3OUc,OjN\|OUW4O\|U9N2F-
HT-29	M3HGb2Z2dmO2aX;uJGF{e2G7	M4HBTVFuVQ>?	M2jRNVQ5Nzl4aB?=		M1;iWIlvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyOEK3Nkc,OjN3MEiyO\|I9N2F-
SH-SY5Y	Mn3RR5l1d3SxeHnjbZR6KEG\|c3H5	MmfPOY1O	NILMV2szPGh?		M3u2bolv[3KnYYPld{BRS05idH;4bYNqfHl?	NELTVHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyOVI3PSd-MkO1NlUzPjV:L3G+
Saos-2	MVrBdI9xfG:\|aYOgRZN{[Xl?	MWixcW0>	MYG5Oog>		NYi2N2ljcW6lcnXhd4V{KGOnbHyg[IVifGhiaX7keYNm\CCkeTDQR3g>	NI[1OJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N\|E4OSd-MkO1OlMyPzF:L3G+
1321N1	MVXDfZRwfG:6aXPpeJkhSXO\|YYm=	M4DwfFVuVQ>?	M3P6UVI1cA>?		M{e3eZBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?=	M3fSUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUK1NlY2Lz5{M{WyOVI3PTxxYU6=
A2780	M{XpT2Fxd3C2b4Ppd{BCe3OjeR?=	NHTPPWc2dU1?	NXHj[Xh4OjSq		Mn3NZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN?	NFno[IY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5NlI5OSd-MkO1PVIzQDF:L3G+
OV2008	NEXGfZJCeG:ydH;zbZMhSXO\|YYm=	NVHFeYgyPW2P	MnrGNlRp		NUTJUpM4[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS\|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO=	MknTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUKyPFEoRjJ\|NUmyNlgyRC:jPh?=
PC12	M2fCb2Z2dmO2aX;uJGF{e2G7	NU\IfZlIOTCvTR?=	M3\6VlI1cA>?	Mnzte4F1\XJ?	NV\KenJbyqCrbnjpZol1eyClaIntc5RzgXC\|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6=	M4[5ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkCzPVc6Lz5{M{[wN\|k4QTxxYU6=
SH-SY5Y	MXLBdI9xfG:\|aYOgRZN{[Xl?	MWC1cW0>	MnfuNYg>		NFTvXGpi[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S=	NYnJVpRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVk{QTVpPkKzOlE6Ozl3PD;hQi=>
SH-SY5Y	NX:5WG1PTnWwY4Tpc44hSXO\|YYm=	MYixcW0>	MUWyOIg>		MmO0bY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IGTPR3A>	M2TmclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{SzNVQ5Lz5{M{e0N\|E1QDxxYU6=
HepG2	MYPGeY5kfGmxbjDBd5NigQ>?	NGK4W\|cyOG2P	MVmyOIg>		NFXJ[I9qdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=>	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzN{C0NEc,OjN6MUewOFA9N2F-
HeLa	NGWxNnhHfW6ldHnvckBCe3OjeR?=	NHLsWlUyOG2P	NEn3S4czcA>?		MlHnd5VxeHKnc4Pld{BNSzNiSVmg[ZhxemW\|c3nzc44>	MmXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS3N\|goRjJ\|OE[0O\|M5RC:jPh?=
HONE-1	M4\jN2Z2dmO2aX;uJGF{e2G7	NHPYZ5Q2dU1?	NGjPSG0ycA>?		NV;zbJJUemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v	NX7KSFc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PVI{PThpPkKzPFkzOzV6PD;hQi=>
MCF7	MVjGeY5kfGmxbjDBd5NigQ>?	M{m2Z\|VuVQ>?	M4XXXlI1cA>?		M3KyTIlv[3KnYYPld{BEfU9iaX7keYNm\CClZXzsJIRm[XSq	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4Mk[yPUc,OjN7NkK2Nlk9N2F-
HO8910	MV7BdI9xfG:\|aYOgRZN{[Xl?	NFf3VncyOG2P	M4DBU\|EzcA>?		MnTB[Y5p[W6lZYOgRlE6NWmwZIXj[YQh[XCxcITvd4k>	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6M{[xNEc,OjN7OEO2NVA9N2F-
SMMC-7721	NIfZd2lCeG:ydH;zbZMhSXO\|YYm=	MWq1cW0>	MV2yOIg>		MWjheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:\|aYO=	NUC4cI17RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl\|PD;hQi=>
Hep3B	NEX3bpFCeG:ydH;zbZMhSXO\|YYm=	MkHjOY1O	NIHZN4QzPGh?		NInGRllifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM>	MmHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNk[2PVMoRjJ2ME[2Olk{RC:jPh?=
H460	NEnqbGFHfW6ldHnvckBCe3OjeR?=	M4LmS\|ExdU1?	MW[0bC=>		MljvbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg>	NWfqb2tKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|MzODhpPkK0NVc{OjB6PD;hQi=>
A549	NVTIXG9nTnWwY4Tpc44hSXO\|YYm=	MWexNI1O	NF7EU5I1cA>?		M3LTTolvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w	M2DyV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUSyO\|M2Lz5{NEG0Nlc{PTxxYU6=
H1299	MmjFSpVv[3Srb36gRZN{[Xl?	NXnuPWR3OTCvTR?=	NIHVcZc1cA>?		MXLpcoNz\WG\|ZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=>	NUPuTXNpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|MzODhpPkK0NVc{OjB6PD;hQi=>
WiDr	NUnES3hSTnWwY4Tpc44hSXO\|YYm=	MV[xNI1O	NFXwc2cycA>?		MnXZbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v	M1nldFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUmwOFg6Lz5{NEG5NFQ5QTxxYU6=
LoVo	Mlz6RZBweHSxc3nzJGF{e2G7	NFS5SGc2dU1?	NXXEV5d4PDiq		M4XWO4VvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5?	NXfoXIJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFE5OTJpPkK0NlAyQDF{PD;hQi=>
HepG2 E47	NI\sbmdHfW6ldHnvckBCe3OjeR?=	M2rlZVIvPW2P	NHPJeHY1QGh?		Mle2bY5kemWjc3XzJJRp\SC2b4jpZ4l1gSCxZjDBRUwhSlORLDDhcoQhS0OuNB?=	NIHQOVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3N\|c{QCd-MkSyO\|M4Ozh:L3G+
RKO	MVrGeY5kfGmxbjDBd5NigQ>?	NEPuS3gzdU1?	NYrJZ3JqOWh?	MmLXSG1UVw>?	M1X6d4VvcGGwY3XzJINmdGxiZHXheIgh[nliZ3Xs[IFv[W27Y3nu	NF;nVG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5NVc4Pyd-MkSyPVE4Pzd:L3G+
Hep3B	MmLyRZBweHSxc3nzJGF{e2G7	MVKycW0>	NGqxc\|YyOmh?	NIC3[m9FVVOR	MYLpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?=	M4DJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N\|AxLz5{NEK5O\|MxODxxYU6=
ACHN-5968	MUHBdI9xfG:\|aYOgRZN{[Xl?	MmXuOY1O	NEDxfo0{cA>?		MlH6[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>?	Mlq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|MEW2NFQoRjJ2M{C1OlA1RC:jPh?=
Huh7	NYPzRpdZSXCxcITvd4l{KEG\|c3H5	NF74eIczdU1?	MWWxNog>	MnrySG1UVw>?	M1fyOIlvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo	MoG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N\|AxRC:jPh?=
UOK257	MYjBdI9xfG:\|aYOgRZN{[Xl?	NFTYTGo2dU1?	NYG1SVFGO2h?		Mk\F[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>?	NVG1bVJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFU3ODRpPkK0N\|A2PjB2PD;hQi=>
ECSCs	NIj3dmhCeG:ydH;zbZMhSXO\|YYm=	NX;sOGdvOTCvTR?=	MYG0bC=>		MW\k[YNz\WG\|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzOUGwPUc,OjR\|MUmxNFk9N2F-
MCF-7	M36wfWZ2dmO2aX;uJGF{e2G7	MmT1NVBuVQ>?	MVqyOIg>		MkTLbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?=	M{H3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G1OVc5Lz5{NEOxOVU4QDxxYU6=
SGC-7901	MojZRZBweHSxc3nzJGF{e2G7	MX:ycW0>	MlrkNYg>		M3nLbIlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m\|	NHf3VlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyNVM1OCd-MkSzNlE{PDB:L3G+
SMMC-7721	MkTuRZBweHSxc3nzJGF{e2G7	MXyycW0>	MUKxbC=>		MXHpcoNz\WG\|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>?	NWnBS4dwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE{PDBpPkK0N\|IyOzRyPD;hQi=>
T24	MWDBdI9xfG:\|aYOgRZN{[Xl?	MWK1cW0>	MUmxMlVp		M173[5BwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>?	MmHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NEmxO\|YoRjJ2M{S5NVc3RC:jPh?=
NTUB1	M3S4OmFxd3C2b4Ppd{BCe3OjeR?=	NFGwdY02dU1?	NH7zNpQyNjWq		MorzdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{	NU[0dGF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N\|Q6OTd4PD;hQi=>
MG-63	NHLIcolCeG:ydH;zbZMhSXO\|YYm=	M1m2d\|ExdU1?	Ml\INVJp		NVnOPFB4\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN?	NVzqe5M6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVg{ODFpPkK0N\|U5OzBzPD;hQi=>
MG-63	NEPYZnlCeG:ydH;zbZMhSXO\|YYm=	MU[wMlUwOW2P	MnL6N\|Jp		MUnlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m\|	MnvXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NUizOFIoRjJ2M{W4N\|QzRC:jPh?=
MG-63	M1jnR2Z2dmO2aX;uJGF{e2G7	NFrUbWYxNjVxMX3N	NF;OTos1QGh?		NH33ZmZqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>?	NEnLUYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
U2OS	MoHSSpVv[3Srb36gRZN{[Xl?	NYK1NYFIOC53L{HtUS=>	NV\pdpVkPDiq		NEHxT29qdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>?	NXnZUndJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVg{PDJpPkK0N\|U5OzR{PD;hQi=>
HGC-27	Ml\zSpVv[3Srb36gRZN{[Xl?	NEXhPFcyOG2P	NFGyTG0ycA>?		MmDLbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIxwe3NiYomgVmFFODBzIH;yJG1MNTJ{ME[=	NVXpRXVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NVY{PDlpPkK0OFE3OzR7PD;hQi=>
HCT116	NYHWTYlLSXCxcITvd4l{KEG\|c3H5	M1vMTlVuVQ>?	NFPIdnczPGh?		MWjlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkWyNkc,OjR4Mk[1NlI9N2F-
A549	M1fZVGFxd3C2b4Ppd{BCe3OjeR?=	M1H2bVExdU1?	MnfxOFhp		NUjyW\|hY[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkeyNkc,OjR4Mk[3NlI9N2F-
Saos-2	NUPxZWQ4SXCxcITvd4l{KEG\|c3H5	M1qzW\|ExdU1?	NEDOWVUzPGh?		M1Hkd4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>?	NXPycWxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|kxOTNpPkK0OlM6ODF\|PD;hQi=>
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HepG2	NUTicoFNTnWwY4Tpc44hSXO\|YYm=	M4\NU\|VuVQ>?	NFPQNWw1cA>?		NUPBSlBQcW6lcnXhd4V{KEiOIILlcIVie2V?	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzM{W4O{c,OjR5MUO1PFc9N2F-
A549	M37VRmFxd3C2b4Ppd{BCe3OjeR?=	M{XoZlVuVQ>?	NXrL[4ZWPDiq		M4rpOIRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuIHTlZZRpKGGwZDDlfJBz\XO\|aX;uJIxmfmWuczDv[kBk[XOyYYPlMVMtKEKnY3zpck0yKGGwZDDMR\|MuUUl?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdyNkOwN{c,OjR5ME[zNFM9N2F-
A2780cp	M3r4ZWFxd3C2b4Ppd{BCe3OjeR?=	MUeyMlVuVQ>?	MXOxbC=>	MkO2[IRJOk9?	MoC3[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=>	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzN{m0Okc,OjR6MUe5OFY9N2F-
Microglia	MWDBdI9xfG:\|aYOgRZN{[Xl?	NGSwUpI2dU1?	MUmyOIg>		MULk[YNz\WG\|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi=	NGfROZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixPFYxOSd-MkS4NVg3ODF:L3G+
HT-29	MVLBdI9xfG:\|aYOgRZN{[Xl?	NEnTO5IydU1?	NYnwfXZ6PDiq	MnWzSG1UVw>?	M3nqNIVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MkG1PEc,OjR6NEKxOVg9N2F-
MDR	MW\BdI9xfG:\|aYOgRZN{[Xl?	NVXDO3VZOTCvTR?=	MUW2bC=>		NWDFeYNTe3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O=	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzOUewNUc,OjVyMUm3NFE9N2F-
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A2780cp	NH7HUFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4PHcFFuVQ>?	M4O1ZVFp		NWnFNYZ1cW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh?	M{jCdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{KyOlk1Lz5{NUOyNlY6PDxxYU6=
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A549	MoTsRZBweHSxc3nzJGF{e2G7	MXW1cW0>	M1TBZVJp	NWL0OHJQTE2VTx?=	NXPy[49pcW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNkW2NUc,OjV5MU[1OlE9N2F-
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A549	NX7ac3BZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGT4T5I{dU1?	NIq4cJozcA>?	M{S0O2ROW09?	MYry[YR2[2W\|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E	M2i1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G2OVYyLz5{NUexOlU3OTxxYU6=
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Nara-H	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{WwUVVuVQ>?	MX:0PIg>		NF23THBmdmijbnPld{B1\W2\|aYLvcIlufXOvZXTpZZRm\CC\|dYDwdoV{e2mxbjDv[kBP[XKjLVigZ4VtdCCycn;sbYZmemG2aX;u	NEnXSJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwOVA{Oyd-MkG4NFUxOzN:L3G+
HUVECs	NH;xOHBHfW6ldHnvckBCe3OjeR?=	NI\STmIyOG2P	Mli5NE42cA>?		MkjV[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?=	Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2Nki1PVIoRjJzNE[4OVkzRC:jPh?=
HepG2	NF\HcWtCeG:ydH;zbZMhSXO\|YYm=	NUj6d2JvOm2P	MVmxbC=>		MnTW[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR3M{[5NUc,OjF2NUO2PVE9N2F-
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A673	M2rpZ5FJXFNiYYPzZZk>				NHnUV4pyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?=	NEPTcFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
DAOY	M2fmcJFJXFNiYYPzZZk>				NHuxSmxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?=	NITjNXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
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BT-37	NGLtOnlyUFSVIHHzd4F6				MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN?	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
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BT-12	MmfRdWhVWyCjc4PhfS=>				M2jtepFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?=	M{fRcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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OHS-50	NGPWUJJyUFSVIHHzd4F6				NVficFR{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF;IV{02OCClZXzsdy=>	NHj5R2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
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Rh30	MYPxTHRUKGG\|c3H5				NV7HdI5JeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGLoN\|Ah[2WubIO=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SJ-GBM2	M4jiWZFJXFNiYYPzZZk>				NXfYR49OeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM>	M4[2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC	M4Dp[ZFJXFNiYYPzZZk>				NWPxSWNVeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM>	NWfyWnJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1	NV7J[XBkeUiWUzDhd5NigQ>?				NVrMWpVTeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM>	Mk\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
LAN-5	MWDxTHRUKGG\|c3H5				NEH2e2tyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO=	M1HYPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18	M4jSSpFJXFNiYYPzZZk>				M2jqbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|	MmPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 caspase-3 / caspase-9 / PARP VEGF APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2	29296191 25891159 26316755
Immunofluorescence	LC3 / Hif-α / COX2	29039446
Growth inhibition assay	Cell viability	26934124

体内研究

3-Methyladenine (3-MA)可以通过对磷酸肌醇3磷酸激酶(PI3K) 的作用来阻断自吞噬, 而PI3K的活性对于自噬体形成早期膜池的成核和组装是必须的。与SAH 处理组相比3-MA并不会改变出血的程度。与SAH +对照成分组相比经过3-MA预处理后会明显加重神经病学症状。3-MA处理会减少自吞噬发生^[5]。相反地,在SAH + 3-MA组里断裂的半胱天冬酶表达量明显上调，与此一致的是与SAH + 对照成分组相比，SAH + 3-MA组中右脑皮层里原位末端标记阳性细胞数量明显增多^[5]。

激酶实验：^[4]	Protein degradation assay: HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U-¹⁴C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
细胞实验：^[2]	Cell lines: HeLa 细胞系 Concentrations: 1-10 mM Incubation Time: 3 分钟 Method: 用台盼蓝染色法分析细胞存活率（如HeLa细胞）。简言之, 经过3-MA处理后将贴壁的与漂浮的细胞都用PBS( pH 7.4)重悬，密度控制在每毫升1-2×10⁶。加入等体积的0.4% 台盼蓝并彻底混匀。室温孵育3分钟后用血细胞计数器对细胞计数分析。
动物实验：^[5]	Animal Models: 体重 300-350 g 的成年雄性Sprague–Dawley 大鼠 Dosages: 400 nM Administration: 脑室给药

参考文献	[1] Miller S, et al. Autophagy. 2010, 6(6), 805-807. [2] Hou H, et al. PLoS One. 2012, 7(4), e35665. [3] Liu Y, et al. J Pharmacol Sci. 2012, 118(4), 423-432. [4] Wu YT, et al. J Biol Chem. 2010, 285(14), 10850-10861. [5] Jing CH, et al. Neuroscience. 2012. + 展开

溶解度（25°C）

体外

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量	149.15
化学式	C₆H₇N₅
CAS号	5142-23-4
储存条件	3年 -20°C 粉状

Download 3-Methyladenine (3-MA) SDF

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

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常见问题及建议解决方法

问题 1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

回答：
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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