3-Methyladenine (3-MA)

目录号:S2767 别名: NSC 66389 中文名称:3-甲基腺嘌呤

仅限科研使用

3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用(加热助溶)

3-Methyladenine (3-MA) Chemical Structure

CAS: 5142-23-4

规格 价格 库存 购买数量
RMB 579.66 现货
RMB 3010.37 现货
大包装 有超大折扣
大剂量询单有大折扣!

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的3-Methyladenine (3-MA)发表文献693篇:

产品安全说明书

PI3K抑制剂选择性比较

相关PI3K产品

生物活性

产品描述 3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用(加热助溶)
靶点
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
体外研究

3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的[1] 。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵,这表明3-MA 除了抑制自吞噬之外还有其它生物功能。3-MA可以造成半胱天冬酶依赖的细胞死亡,这一功能与它对自吞噬的抑制无关。用5mM 3-MA处理葡萄糖饥饿Hela细胞可降低gfp-lc3阳性细胞比例至23%。3-MA处理细胞12到48小时内 LC3-I 的水平上升而LC3-II 水平下降。LC3-I转为为 LC3-II 的过程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 处理HeLa 细胞一天并不影响细胞存活率, 而 10 mM 3-MA 处理一天会造成细胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 处理细胞两天分别使细胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低细胞存活率的作用具有时间和剂量依赖特性。3-MA 明显缩短nocodazole诱导的前中期阻断时间[2]。3-MA 通过抑制自吞噬来抑制SU11274诱导的细胞死亡[3]。 在野生型MEF细胞中延长 3-MA处理时间 (最多 9 小时) 明显造成 LC3 I 到 II 转换。延长 3-MA处理时间会明显增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介导的LC3 转换和游离 GFP 释放是ATG7依赖的。 3-MA处理会导致 p62 蛋白水平明显升高。即使在Atg5−/− 的MEF细胞中3-MA 也会使p62 水平升高,就像在DOX介导的ATG5缺失的细胞中一样 。3-MA以不同方式抑制 I 型和 III型 PI3K。与野生型细胞相比,在Tsc2−/−细胞中3-MA诱导的 LC3 I 到 LC3 II 转换过程大幅下降。3-MA会破坏mTOR复合物1的拮抗自吞噬功能[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 NFfudoZHfW6ldHnvckBCe3OjeR?= NF;nfI0yOG2P NYGwVmhTOWh? M2jIUIRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= M2HpS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
Jurkat MV7GeY5kfGmxbjDBd5NigQ>? NUDjPJc1OTCvTR?= MoexNYg> M4L1T4Rm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= M3G2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
HeLa  Moj5R5l1d3SxeHnjbZR6KEG|c3H5 MXqycW0> NInYcYEzPGh? NHTI[FJqdmirYnn0[ZMhfGinIHP5eI91d3irY3n0fUBw\iC|aXzpZolvcW5idH:gTIVN[SClZXzsd{4> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5NUO4OUc,OjF6N{WzPFU9N2F-
PC12/TetOn M3ey[GZ2dmO2aX;uJGF{e2G7 NGP6[HkxNjFxMX3N NXnTcVFIOTiq NUDzTYhidGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= NIjRZZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwOlY2QSd-MkG5NFY3PTl:L3G+
RMPI8226  MUTGeY5kfGmxbjDBd5NigQ>? MmrDOY1O M17Hb|Fp NYD2WJZje3WycILld5NmeyC2aHWgcIV3\Wxib3[gZZV1d3CqYXf5JJVv\GW{IH71eJJq\W62IHTldIxmfGmxbh?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlzNU[yNEc,OjF7MUW2NlA9N2F-
MCF-7 NGrwdGJHfW6ldHnvckBCe3OjeR?= MmfoNVBuVQ>? NIfoPGs1QGh? Mn\oZoxw[2u|IHH1eI9xcGGpeTDpcoR2[2WmIHL5JIJwenSnen;tbYI> MlfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7M{G5N|coRjJzOUOxPVM4RC:jPh?=
HBx  NIL1THRCeG:ydH;zbZMhSXO|YYm= NHfsRngyOG2P MYG0PIg> MXfEUXNQ MnXXbY5kemWjc3XzJINmdGxiZHXheIg> Mn;nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkCwO|goRjJ{MEKwNFc5RC:jPh?=
Marc-145 MVjGeY5kfGmxbjDBd5NigQ>? NWXifWVwPW2P NE\ZWoEyOi9{ND:zOog> MmrWdoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937 NXzB[FhsTnWwY4Tpc44hSXO|YYm= MlXWNo1O MnrDNVJp M3ruPYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MoD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNUWxOVAoRjJ{MUW1NVUxRC:jPh?=
BGC-823 M1zqNWZ2dmO2aX;uJGF{e2G7 M1e4VFVuVQ>? NGX5[|IzcA>? NYPjbWNLcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? NG\6OWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNlE2Oid-MkKzNlIyPTJ:L3G+
A549 MVXGeY5kfGmxbjDBd5NigQ>? NIrxVHAxNjGvTR?= M3TobFI1cA>? M37nTZN2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4Nkm2NEc,OjJ2Nk[5OlA9N2F-
pDCs M4jnRWZ2dmO2aX;uJGF{e2G7 NUjubYVCOTCvTR?= MnX6NE42cA>? NFPRcHnDqHKnZIXj[ZMhfGinIHnu[JVkfGmxbjDv[kBKTk5vzsGgZpkhe3OUTlG0NC=> M4TYVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{m2OVk6Lz5{MkO5OlU6QTxxYU6=
HeLa  NFPKfHhCeG:ydH;zbZMhSXO|YYm= MmHqOY1O NYHxdmdXOjSq MknQbY5lfWOnczDjZZNx[XOnLXTldIVv\GWwdDDj[YxtKGSnYYTo NVPTb3dNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFUyOjhpPkKyOVQ2OTJ6PD;hQi=>
U251  NIq2T5BCeG:ydH;zbZMhSXO|YYm= NIjQWpU2dU1? M130NVI1cA>? M165V4lv[3KnYYPld{BUOS2rbnT1Z4VlKGOnbHyg[IVifGh? NYn5cYJmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1O|k4QDhpPkKyOVc6Pzh6PD;hQi=>
MCF-7  Ml7zRZBweHSxc3nzJGF{e2G7 NXi2NoJOOC5zbV2= M1;hWlZp NULONXdn\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> MmXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NUG5PFkoRjJ{N{WxPVg6RC:jPh?=
PC-3  NHTXRpJCeG:ydH;zbZMhSXO|YYm= NILpNmwzdU1? NVPpVWY6Omh? NWm0dYNWcW6lcnXhd4V{KE:UST3pcoR2[2WmIHPlcIwh\GWjdHi= NHX3cJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0OVU5OCd-MkK3OFU2QDB:L3G+
HCT116 NHWzWY1CeG:ydH;zbZMhSXO|YYm= NUSxSoVDPW2P NGjWXoMzPGh? NHz0WmJFVVOR NUjlNGd2\W6qYX7j[ZMh[XCrZ3XubY4ucW6mdXPl[EBk\WyuIHTlZZRp MljLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
U2OS NH\hT25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DRe|ExdU1? MWWyOIg> MXnpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g> NWrvT2tSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
A2780cp NU\LOFY3SXCxcITvd4l{KEG|c3H5 MkfiNk42dU1? MmT1NYg> MUjk[GgzVw>? NX;sXZpu\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M2PrT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG3PVQ3Lz5{NEixO|k1PjxxYU6=
HepG2 M3LjcWZ2dmO2aX;uJGF{e2G7 MYS1cW0> M{HycFRp M{T6UYlv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= NX\5[mYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O|E{PTh5PD;hQi=>
Microglia  Mn\MRZBweHSxc3nzJGF{e2G7 M3jZclVuVQ>? NFLFWWMzPGh? MWXk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= M2rCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG4OlAyLz5{NEixPFYxOTxxYU6=
MDA-MB 231 Mnm1RZBweHSxc3nzJGF{e2G7 MkTlOY1O MnjXNE42cA>? NITuO4Zud2S3bHH0[ZMhXG:lb33pcuKvyqCrbnT1Z4VlKGGyb4D0c5Nqew>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|MEe4NUc,OjR6M{C3PFE9N2F-
PANC-1  NIi5TYpCeG:ydH;zbZMhSXO|YYm= MYixcW0> Mly0OFhp Mo\qSG1UVw>? NYK1VFVm\W6qYX7j[ZMh[m:{dHX6c41q[i2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? NWLKZmFkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OFIyPThpPkK0PFQzOTV6PD;hQi=>
MDA-MB-231 M2\6SmZ2dmO2aX;uJGF{e2G7 NXztVY5WOm2P MlvwOFhp NEXCWWxxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? NIfl[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O|A3PzZ:L3G+
MDA-MB-231 MkLTSpVv[3Srb36gRZN{[Xl? M{fZZ|JuVQ>? NH35cIYzPGh? MYHpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NEnjOGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O|A3PzZ:L3G+
MCF-7  M{DNOmZ2dmO2aX;uJGF{e2G7 MVWycW0> M3WyWVQ5cA>? M170ZpBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? MlTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7N{C2O|YoRjJ2OUewOlc3RC:jPh?=
MCF-7  NGnRUpBHfW6ldHnvckBCe3OjeR?= NXX1b5VzOm2P NH;PbWgzPGh? M4XqSYlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5ME[3Okc,OjR7N{C2O|Y9N2F-
HepG2 MmHKRZBweHSxc3nzJGF{e2G7 MmDrN41O MWS1bC=> NFjFVlJz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgVWR{ NHG1d|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizOlU6PSd-MkK4N|Y2QTV:L3G+
HeLa NFLlWIlCeG:ydH;zbZMhSXO|YYm= NXjvS4Q4OTCvTR?= MYSybC=> NHfSWXdl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK M13CdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MECwNVM2Lz5{M{CwNFE{PTxxYU6=
SK-HEP-1 M{XkWWFxd3C2b4Ppd{BCe3OjeR?= MnfhNVBuVQ>? MU[xbC=> MYLwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz NXTmdndkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OVg3PDlpPkKyPFU5PjR7PD;hQi=>
MDA-MB231 NH7jPYVHfW6ldHnvckBCe3OjeR?= MmfYOY1O MY[xbC=> MV;pcoNz\WG|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyOEi4OVAoRjJ|MEi4PFUxRC:jPh?=
PaCa44 M33hNGFxd3C2b4Ppd{BCe3OjeR?= M{PaWlIvPW2P Ml3yNYg> NHqwdo9z\WS3Y3XzxsBo\W6rcHnuMY1m\GmjdHXkJIFxd3C2b4Ppdy=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF{NEGxNkc,OjNzMkSxNVI9N2F-
T-47D MVjGeY5kfGmxbjDBd5NigQ>? MVexNI1O NXvZZVdVOmh? NY[0cIN7cW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:|aYO= M3HOfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{CwNFI3Lz5{M{OwNFAzPjxxYU6=
GTL-16  NFHTZY1CeG:ydH;zbZMhSXO|YYm= MVm1cW0> Mlz3NlRp M{\EUpJm\HWlZYOgZ4VtdCC4aXHibYxqfHliYYOgZ49ueGG{ZXSgeI8h[2WubIOgeJJm[XSnZDD3bZRpKE2HVDDpcohq[mm2b4Lz MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN|MUO0PVA9N2F-
U251MG  M4jwXmZ2dmO2aX;uJGF{e2G7 MV2zcW0> M2nJe|Fp NFLCeFZ{fXCycnXzd4V{KEyFMz3JTUBxem:2ZXnuJIV5eHKnc4Ppc44> NH71UHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OzPFYyQCd-MkOzN|g3OTh:L3G+
T24 MUfGeY5kfGmxbjDBd5NigQ>? MmriNVBuVQ>? M{frO|Fp MmPydoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3NEC4NEc,OjN|NUSwPFA9N2F-
HUVECs NUnkUFBoTnWwY4Tpc44hSXO|YYm= M1fT[|NuVQ>? M3fRNFI1cA>? MYHicI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6 NIrlRXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1PFkzQCd-MkOzOVg6Ojh:L3G+
MCF-7  MWLGeY5kfGmxbjDBd5NigQ>? MXS1cW0> Ml70NlRp M3zHW4lvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OUW2O|koRjJ|M{m1Olc6RC:jPh?=
Hela  NWTqNIN4TnWwY4Tpc44hSXO|YYm= MUO1cW0> NFGxe4QzPGh? MnTFbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> NHO1fFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVY4QSd-MkOzPVU3Pzl:L3G+
OR6 MXLGeY5kfGmxbjDBd5NigQ>? MnXCNVBuVQ>? NEDlb484Omh? M3\5Z5N2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NUi3OUc,OjN|OUW4O|U9N2F-
HT-29  M3HGb2Z2dmO2aX;uJGF{e2G7 M4HBTVFuVQ>? M2jRNVQ5Nzl4aB?= M1;iWIlvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyOEK3Nkc,OjN3MEiyO|I9N2F-
SH-SY5Y Mn3RR5l1d3SxeHnjbZR6KEG|c3H5 MmfPOY1O NILMV2szPGh? M3u2bolv[3KnYYPld{BRS05idH;4bYNqfHl? NELTVHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyOVI3PSd-MkO1NlUzPjV:L3G+
Saos-2  MVrBdI9xfG:|aYOgRZN{[Xl? MWixcW0> MYG5Oog> NYi2N2ljcW6lcnXhd4V{KGOnbHyg[IVifGhiaX7keYNm\CCkeTDQR3g> NI[1OJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N|E4OSd-MkO1OlMyPzF:L3G+
1321N1 MVXDfZRwfG:6aXPpeJkhSXO|YYm= M4DwfFVuVQ>? M3P6UVI1cA>? M{e3eZBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= M3fSUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK1NlY2Lz5{M{WyOVI3PTxxYU6=
A2780 M{XpT2Fxd3C2b4Ppd{BCe3OjeR?= NHTPPWc2dU1? NXHj[Xh4OjSq Mn3NZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? NFno[IY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5NlI5OSd-MkO1PVIzQDF:L3G+
OV2008 NEXGfZJCeG:ydH;zbZMhSXO|YYm= NVHFeYgyPW2P MnrGNlRp NUTJUpM4[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= MknTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUKyPFEoRjJ|NUmyNlgyRC:jPh?=
PC12  M2fCb2Z2dmO2aX;uJGF{e2G7 NU\IfZlIOTCvTR?= M3\6VlI1cA>? Mnzte4F1\XJ? NV\KenJbyqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= M4[5ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCzPVc6Lz5{M{[wN|k4QTxxYU6=
SH-SY5Y MXLBdI9xfG:|aYOgRZN{[Xl? MWC1cW0> MnfuNYg> NFTvXGpi[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= NYnJVpRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVk{QTVpPkKzOlE6Ozl3PD;hQi=>
SH-SY5Y NX:5WG1PTnWwY4Tpc44hSXO|YYm= MYixcW0> MUWyOIg> MmO0bY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IGTPR3A> M2TmclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{SzNVQ5Lz5{M{e0N|E1QDxxYU6=
HepG2 MYPGeY5kfGmxbjDBd5NigQ>? NGK4W|cyOG2P MVmyOIg> NFXJ[I9qdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzN{C0NEc,OjN6MUewOFA9N2F-
HeLa NGWxNnhHfW6ldHnvckBCe3OjeR?= NHLsWlUyOG2P NEn3S4czcA>? MlHnd5VxeHKnc4Pld{BNSzNiSVmg[ZhxemW|c3nzc44> MmXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS3N|goRjJ|OE[0O|M5RC:jPh?=
HONE-1  M4\jN2Z2dmO2aX;uJGF{e2G7 NHPYZ5Q2dU1? NGjPSG0ycA>? NV;zbJJUemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v NX7KSFc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PVI{PThpPkKzPFkzOzV6PD;hQi=>
MCF7 MVjGeY5kfGmxbjDBd5NigQ>? M{m2Z|VuVQ>? M4XXXlI1cA>? M3KyTIlv[3KnYYPld{BEfU9iaX7keYNm\CClZXzsJIRm[XSq MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4Mk[yPUc,OjN7NkK2Nlk9N2F-
HO8910 MV7BdI9xfG:|aYOgRZN{[Xl? NFf3VncyOG2P M4DBU|EzcA>? MnTB[Y5p[W6lZYOgRlE6NWmwZIXj[YQh[XCxcITvd4k> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6M{[xNEc,OjN7OEO2NVA9N2F-
SMMC-7721 NIfZd2lCeG:ydH;zbZMhSXO|YYm= MWq1cW0> MV2yOIg> MWjheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= NUC4cI17RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl|PD;hQi=>
Hep3B NEX3bpFCeG:ydH;zbZMhSXO|YYm= MkHjOY1O NIHZN4QzPGh? NInGRllifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> MmHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNk[2PVMoRjJ2ME[2Olk{RC:jPh?=
H460 NEnqbGFHfW6ldHnvckBCe3OjeR?= M4LmS|ExdU1? MW[0bC=> MljvbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NWfqb2tKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|MzODhpPkK0NVc{OjB6PD;hQi=>
A549 NVTIXG9nTnWwY4Tpc44hSXO|YYm= MWexNI1O NF7EU5I1cA>? M3LTTolvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w M2DyV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUSyO|M2Lz5{NEG0Nlc{PTxxYU6=
H1299  MmjFSpVv[3Srb36gRZN{[Xl? NXnuPWR3OTCvTR?= NIHVcZc1cA>? MXLpcoNz\WG|ZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NUPuTXNpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|MzODhpPkK0NVc{OjB6PD;hQi=>
WiDr NUnES3hSTnWwY4Tpc44hSXO|YYm= MV[xNI1O NFXwc2cycA>? MnXZbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v M1nldFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUmwOFg6Lz5{NEG5NFQ5QTxxYU6=
LoVo  Mlz6RZBweHSxc3nzJGF{e2G7 NFS5SGc2dU1? NXXEV5d4PDiq M4XWO4VvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5? NXfoXIJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFE5OTJpPkK0NlAyQDF{PD;hQi=>
HepG2 E47 NI\sbmdHfW6ldHnvckBCe3OjeR?= M2rlZVIvPW2P NHPJeHY1QGh? Mle2bY5kemWjc3XzJJRp\SC2b4jpZ4l1gSCxZjDBRUwhSlORLDDhcoQhS0OuNB?= NIHQOVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3N|c{QCd-MkSyO|M4Ozh:L3G+
RKO MVrGeY5kfGmxbjDBd5NigQ>? NEPuS3gzdU1? NYrJZ3JqOWh? MmLXSG1UVw>? M1X6d4VvcGGwY3XzJINmdGxiZHXheIgh[nliZ3Xs[IFv[W27Y3nu NF;nVG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5NVc4Pyd-MkSyPVE4Pzd:L3G+
Hep3B MmLyRZBweHSxc3nzJGF{e2G7 MVKycW0> NGqxc|YyOmh? NIC3[m9FVVOR MYLpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= M4DJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
ACHN-5968 MUHBdI9xfG:|aYOgRZN{[Xl? MmXuOY1O NEDxfo0{cA>? MlH6[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? Mlq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MEW2NFQoRjJ2M{C1OlA1RC:jPh?=
Huh7 NYPzRpdZSXCxcITvd4l{KEG|c3H5 NF74eIczdU1? MWWxNog> MnrySG1UVw>? M1fyOIlvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo MoG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
UOK257 MYjBdI9xfG:|aYOgRZN{[Xl? NFTYTGo2dU1? NYG1SVFGO2h? Mk\F[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? NVG1bVJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFU3ODRpPkK0N|A2PjB2PD;hQi=>
ECSCs  NIj3dmhCeG:ydH;zbZMhSXO|YYm= NX;sOGdvOTCvTR?= MYG0bC=> MW\k[YNz\WG|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzOUGwPUc,OjR|MUmxNFk9N2F-
MCF-7  M36wfWZ2dmO2aX;uJGF{e2G7 MmT1NVBuVQ>? MVqyOIg> MkTLbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?= M{H3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G1OVc5Lz5{NEOxOVU4QDxxYU6=
SGC-7901  MojZRZBweHSxc3nzJGF{e2G7 MX:ycW0> MlrkNYg> M3nLbIlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| NHf3VlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyNVM1OCd-MkSzNlE{PDB:L3G+
SMMC-7721 MkTuRZBweHSxc3nzJGF{e2G7 MXyycW0> MUKxbC=> MXHpcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NWnBS4dwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE{PDBpPkK0N|IyOzRyPD;hQi=>
T24 MWDBdI9xfG:|aYOgRZN{[Xl? MWK1cW0> MUmxMlVp M173[5BwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? MmHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEmxO|YoRjJ2M{S5NVc3RC:jPh?=
NTUB1 M3S4OmFxd3C2b4Ppd{BCe3OjeR?= NFGwdY02dU1? NH7zNpQyNjWq MorzdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ NU[0dGF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N|Q6OTd4PD;hQi=>
MG-63 NHLIcolCeG:ydH;zbZMhSXO|YYm= M1m2d|ExdU1? Ml\INVJp NVnOPFB4\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? NVzqe5M6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVg{ODFpPkK0N|U5OzBzPD;hQi=>
MG-63 NEPYZnlCeG:ydH;zbZMhSXO|YYm= MU[wMlUwOW2P MnL6N|Jp MUnlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| MnvXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUizOFIoRjJ2M{W4N|QzRC:jPh?=
MG-63 M1jnR2Z2dmO2aX;uJGF{e2G7 NFrUbWYxNjVxMX3N NF;OTos1QGh? NH33ZmZqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NEnLUYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
U2OS  MoHSSpVv[3Srb36gRZN{[Xl? NYK1NYFIOC53L{HtUS=> NV\pdpVkPDiq NEHxT29qdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NXnZUndJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVg{PDJpPkK0N|U5OzR{PD;hQi=>
HGC-27 Ml\zSpVv[3Srb36gRZN{[Xl? NEXhPFcyOG2P NFGyTG0ycA>? MmDLbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIxwe3NiYomgVmFFODBzIH;yJG1MNTJ{ME[= NVXpRXVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NVY{PDlpPkK0OFE3OzR7PD;hQi=>
HCT116  NYHWTYlLSXCxcITvd4l{KEG|c3H5 M1vMTlVuVQ>? NFPIdnczPGh? MWjlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkWyNkc,OjR4Mk[1NlI9N2F-
A549 M1fZVGFxd3C2b4Ppd{BCe3OjeR?= M1H2bVExdU1? MnfxOFhp NUjyW|hY[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkeyNkc,OjR4Mk[3NlI9N2F-
Saos-2 NUPxZWQ4SXCxcITvd4l{KEG|c3H5 M1qzW|ExdU1? NEDOWVUzPGh? M1Hkd4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? NXPycWxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
U2OS NGSxdVhCeG:ydH;zbZMhSXO|YYm= NUSwb2RxOTCvTR?= MoH2NlRp MV;pcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MonwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{mwNVMoRjJ2NkO5NFE{RC:jPh?=
HepG2 NUTicoFNTnWwY4Tpc44hSXO|YYm= M4\NU|VuVQ>? NFPQNWw1cA>? NUPBSlBQcW6lcnXhd4V{KEiOIILlcIVie2V? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzM{W4O{c,OjR5MUO1PFc9N2F-
A549 M37VRmFxd3C2b4Ppd{BCe3OjeR?= M{XoZlVuVQ>? NXrL[4ZWPDiq M4rpOIRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuIHTlZZRpKGGwZDDlfJBz\XO|aX;uJIxmfmWuczDv[kBk[XOyYYPlMVMtKEKnY3zpck0yKGGwZDDMR|MuUUl? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdyNkOwN{c,OjR5ME[zNFM9N2F-
A2780cp M3r4ZWFxd3C2b4Ppd{BCe3OjeR?= MUeyMlVuVQ>? MXOxbC=> MkO2[IRJOk9? MoC3[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzN{m0Okc,OjR6MUe5OFY9N2F-
Microglia  MWDBdI9xfG:|aYOgRZN{[Xl? NGSwUpI2dU1? MUmyOIg> MULk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= NGfROZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixPFYxOSd-MkS4NVg3ODF:L3G+
HT-29  MVLBdI9xfG:|aYOgRZN{[Xl? NEnTO5IydU1? NYnwfXZ6PDiq MnWzSG1UVw>? M3nqNIVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MkG1PEc,OjR6NEKxOVg9N2F-
MDR MW\BdI9xfG:|aYOgRZN{[Xl? NVXDO3VZOTCvTR?= MUW2bC=> NWDFeYNTe3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzOUewNUc,OjVyMUm3NFE9N2F-
H157 NXroWWxJTnWwY4Tpc44hSXO|YYm= M{njSVVuVQ>? MXmybC=> MlHPd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ6NU[yPEc,OjV{OEW2Nlg9N2F-
A549  MW\GeY5kfGmxbjDBd5NigQ>? MmjoOY1O MmrqNog> M3PoZZN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> M3XQWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mki1OlI5Lz5{NUK4OVYzQDxxYU6=
A2780cp  NH7HUFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PHcFFuVQ>? M4O1ZVFp NWnFNYZ1cW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? M{jCdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{KyOlk1Lz5{NUOyNlY6PDxxYU6=
NBL-W-S  NH;xfVRCeG:ydH;zbZMhSXO|YYm= MoTrNY1O M{i0PFZp NHzlVXpqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBISU6WLU[x MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN{M{KyNkc,OjV|MkOyNlI9N2F-
NBL-W-S  M13NdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1S4blFuVQ>? M2Dwe|Zp NHWzZZZmdmijbnPld{BISU6WLU[xJJRwgGmlaYT5 MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN{M{KyNkc,OjV|MkOyNlI9N2F-
A549  MoTsRZBweHSxc3nzJGF{e2G7 MXW1cW0> M1TBZVJp NWL0OHJQTE2VTx?= NXPy[49pcW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNkW2NUc,OjV5MU[1OlE9N2F-
95D M{HRVmFxd3C2b4Ppd{BCe3OjeR?= MnPrOY1O M3nmTFJp NETnb4xFVVOR NGTSbodqdmirYnn0d{BDTE2FLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHi= NGPQ[FA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUexOlU3OSd-MkW3NVY2PjF:L3G+
A549  NX7ac3BZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGT4T5I{dU1? NIq4cJozcA>? M{S0O2ROW09? MYry[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E M2i1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G2OVYyLz5{NUexOlU3OTxxYU6=
95D MnfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\JdZA{dU1? NF\SO3QzcA>? MnrpSG1UVw>? NHzNZ41z\WS3Y3XzJIdzd3e2aDDpcohq[mm2b4L5JIVn\mWldDDv[kBDTE2F Ml7HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MU[1OlEoRjJ3N{G2OVYyRC:jPh?=
Nara-H MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{WwUVVuVQ>? MX:0PIg> NF23THBmdmijbnPld{B1\W2|aYLvcIlufXOvZXTpZZRm\CC|dYDwdoV{e2mxbjDv[kBP[XKjLVigZ4VtdCCycn;sbYZmemG2aX;u NEnXSJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwOVA{Oyd-MkG4NFUxOzN:L3G+
HUVECs NH;xOHBHfW6ldHnvckBCe3OjeR?= NI\STmIyOG2P Mli5NE42cA>? MkjV[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?= Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2Nki1PVIoRjJzNE[4OVkzRC:jPh?=
HepG2 NF\HcWtCeG:ydH;zbZMhSXO|YYm= NUj6d2JvOm2P MVmxbC=> MnTW[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR3M{[5NUc,OjF2NUO2PVE9N2F-
U-2 OS NE\CXFlyUFSVIHHzd4F6 MlPodWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFVvMjDPV{Bk\Wyucx?= NHzvW2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 M2rpZ5FJXFNiYYPzZZk> NHnUV4pyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= NEPTcFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY M2fmcJFJXFNiYYPzZZk> NHuxSmxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= NITjNXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 M1u4SJFJXFNiYYPzZZk> MVfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| NW[4eHFJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 NGLtOnlyUFSVIHHzd4F6 MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD M2CzfJFJXFNiYYPzZZk> M{\rb5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? NG\4Omc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH MnOwdWhVWyCjc4PhfS=> NVv3eJV3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 MmfRdWhVWyCjc4PhfS=> M2jtepFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= M{fRcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) NWjLbZVPeUiWUzDhd5NigQ>? NEHYbYVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= NF7mSYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 Mk[2dWhVWyCjc4PhfS=> MkP5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 NGPWUJJyUFSVIHHzd4F6 NVficFR{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF;IV{02OCClZXzsdy=> NHj5R2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 MmDLdWhVWyCjc4PhfS=> NHHBO2JyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= NWLxNm1vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh30 MYPxTHRUKGG|c3H5 NV7HdI5JeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoN|Ah[2WubIO= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 M4jiWZFJXFNiYYPzZZk> NXfYR49OeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> M4[2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC M4Dp[ZFJXFNiYYPzZZk> NWPxSWNVeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NWfyWnJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 NV7J[XBkeUiWUzDhd5NigQ>? NVrMWpVTeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> Mk\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 MWDxTHRUKGG|c3H5 NEH2e2tyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M1HYPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 M4jSSpFJXFNiYYPzZZk> M2jqbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy| MmPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Assay
Methods Test Index PMID
Western blot α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 caspase-3 / caspase-9 / PARP VEGF APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2 29296191 25891159 26316755
Immunofluorescence LC3 / Hif-α / COX2 29039446
Growth inhibition assay Cell viability 26934124
体内研究 3-Methyladenine (3-MA)可以通过对磷酸肌醇3磷酸激酶(PI3K) 的作用来阻断自吞噬, 而PI3K的活性对于自噬体形成早期膜池的成核和组装是必须的。与SAH 处理组相比3-MA并不会改变出血的程度。与SAH +对照成分组相比经过3-MA预处理后会明显加重神经病学症状。3-MA处理会减少自吞噬发生[5]。 相反地,在SAH + 3-MA组里 断裂的半胱天冬酶表达量明显上调,与此一致的是与SAH + 对照成分组相比,SAH + 3-MA组中右脑皮层里原位末端标记阳性细胞数量明显增多[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[4]
  • Protein degradation assay:

    HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.

细胞实验:[2]
  • Cell lines: HeLa 细胞系
  • Concentrations: 1-10 mM
  • Incubation Time: 3 分钟
  • Method: 用台盼蓝染色法分析细胞存活率(如HeLa细胞)。简言之, 经过3-MA处理后将贴壁的与漂浮的细胞都用PBS( pH 7.4)重悬,密度控制在每毫升1-2×106。加入等体积的0.4% 台盼蓝并彻底混匀。室温孵育3分钟后用血细胞计数器对细胞计数分析。
动物实验:[5]
  • Animal Models: 体重 300-350 g 的成年雄性Sprague–Dawley 大鼠
  • Dosages: 400 nM
  • Administration: 脑室给药

溶解度(25°C)

体外

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 149.15
化学式

C6H7N5

CAS号 5142-23-4
储存条件 3年 -20°C 粉状

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们

常见问题及建议解决方法

问题 1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

回答:
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.