3-Methyladenine (3-MA)

For research use only. Not for use in humans.

目录号:S2767 别名: NSC 66389 中文名称:3-甲基腺嘌呤

3-Methyladenine (3-MA) Chemical Structure

CAS No. 5142-23-4

3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用(加热助溶)

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RMB 3010.37 现货
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客户使用Selleck生产的3-Methyladenine (3-MA)发表文献591篇:

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂,作用于Vps34PI3Kγ,在 HeLa细胞中IC50分别为25 μM和60 μM;永久性抑制I型PI3K,但对III型PI3K的抑制是短暂的,也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用(加热助溶)
靶点
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
体外研究

3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的[1] 。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵,这表明3-MA 除了抑制自吞噬之外还有其它生物功能。3-MA可以造成半胱天冬酶依赖的细胞死亡,这一功能与它对自吞噬的抑制无关。用5mM 3-MA处理葡萄糖饥饿Hela细胞可降低gfp-lc3阳性细胞比例至23%。3-MA处理细胞12到48小时内 LC3-I 的水平上升而LC3-II 水平下降。LC3-I转为为 LC3-II 的过程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 处理HeLa 细胞一天并不影响细胞存活率, 而 10 mM 3-MA 处理一天会造成细胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 处理细胞两天分别使细胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低细胞存活率的作用具有时间和剂量依赖特性。3-MA 明显缩短nocodazole诱导的前中期阻断时间[2]。3-MA 通过抑制自吞噬来抑制SU11274诱导的细胞死亡[3]。 在野生型MEF细胞中延长 3-MA处理时间 (最多 9 小时) 明显造成 LC3 I 到 II 转换。延长 3-MA处理时间会明显增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介导的LC3 转换和游离 GFP 释放是ATG7依赖的。 3-MA处理会导致 p62 蛋白水平明显升高。即使在Atg5−/− 的MEF细胞中3-MA 也会使p62 水平升高,就像在DOX介导的ATG5缺失的细胞中一样 。3-MA以不同方式抑制 I 型和 III型 PI3K。与野生型细胞相比,在Tsc2−/−细胞中3-MA诱导的 LC3 I 到 LC3 II 转换过程大幅下降。3-MA会破坏mTOR复合物1的拮抗自吞噬功能[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 M1\OUGZ2dmO2aX;uJGF{e2G7 NICzS4QyOG2P MV2xbC=> MmHk[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= Mn3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NkSwN|coRjJzOE[0NFM4RC:jPh?=
Jurkat M2G3XGZ2dmO2aX;uJGF{e2G7 NHPUZWcyOG2P MX6xbC=> MV;k[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh4NECzO{c,OjF6NkSwN|c9N2F-
HeLa  NHfQXnJEgXSxdH;4bYNqfHliQYPzZZk> M1PielJuVQ>? NYH4OFhHOjSq MXjpcohq[mm2ZYOgeIhmKGO7dH;0c5hq[2m2eTDv[kB{cWyrYnnubY4hfG9iSHXMZUBk\Wyucz6= Mk\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{WzPFUoRjJzOEe1N|g2RC:jPh?=
PC12/TetOn MnmxSpVv[3Srb36gRZN{[Xl? NH3xOIExNjFxMX3N Mn61NVhp Mnn5cIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlyNk[1PUc,OjF7ME[2OVk9N2F-
RMPI8226  MVXGeY5kfGmxbjDBd5NigQ>? MnjpOY1O MkG1NYg> MkXkd5VxeHKnc4Pld{B1cGVibHX2[Ywhd2ZiYYX0c5Bp[We7IIXu[IVzKG63dILp[Y51KGSncHzleIlwdg>? MmPhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7MUW2NlAoRjJzOUG1OlIxRC:jPh?=
MCF-7 MoT3SpVv[3Srb36gRZN{[Xl? MV[xNI1O NEHNd3Y1QGh? MVficI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> NETyeng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmzNVk{Pyd-MkG5N|E6Ozd:L3G+
HBx  MWrBdI9xfG:|aYOgRZN{[Xl? M{fyd|ExdU1? MmL3OFhp NFnaU4VFVVOR NWLxOlBJcW6lcnXhd4V{KGOnbHyg[IVifGh? NX3mdXp1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwNlAxPzhpPkKyNFIxODd6PD;hQi=>
Marc-145 NXv6[ol1TnWwY4Tpc44hSXO|YYm= NUX1WVNZPW2P M3nyOFEzNzJ2L{O2bC=> MUPy[YR2[2W|IITo[UBRWlKVVjD0bZRmenNiYX7kJJRp\SCycn;0[YlvKGW6cILld5Nqd25? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937 MX7GeY5kfGmxbjDBd5NigQ>? MYmycW0> NWrXO4U2OTKq MmTl[IVkemWjc3XzJJRp\SCjdYTvdIhi\3licnH0bY/DqA>? MlnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNUWxOVAoRjJ{MUW1NVUxRC:jPh?=
BGC-823 MVrGeY5kfGmxbjDBd5NigQ>? MXy1cW0> NGDnSmszcA>? NHjRPJBqdmirYnn0d{B1cGVicnH0[UBw\iCjdYTvdIhi\2mlIHPlcIx{ MlrDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MkKxOVIoRjJ{M{KyNVUzRC:jPh?=
A549 MlPKSpVv[3Srb36gRZN{[Xl? MV:wMlFuVQ>? M{XZTVI1cA>? M4\aTZN2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg> M2rFOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[2PVYxLz5{MkS2Olk3ODxxYU6=
pDCs MXjGeY5kfGmxbjDBd5NigQ>? M2D6eFExdU1? NIXsVmoxNjWq NFryb3rDqHKnZIXj[ZMhfGinIHnu[JVkfGmxbjDv[kBKTk5vzsGgZpkhe3OUTlG0NC=> NU\2TpB6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzPVY2QTlpPkKyN|k3PTl7PD;hQi=>
HeLa  MUTBdI9xfG:|aYOgRZN{[Xl? MVW1cW0> NYXoSnBkOjSq NX:1RpFDcW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp NUfZeoxFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFUyOjhpPkKyOVQ2OTJ6PD;hQi=>
U251  NIjkRYJCeG:ydH;zbZMhSXO|YYm= M1HYUFVuVQ>? M2\pcVI1cA>? NVr1R5pscW6lcnXhd4V{KFNzLXnu[JVk\WRiY3XscEBl\WG2aB?= M1TaO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUe5O|g5Lz5{MkW3PVc5QDxxYU6=
MCF-7  NHfVTpFCeG:ydH;zbZMhSXO|YYm= NW\oWIV7OC5zbV2= M4LFS|Zp MlXC[Y5p[W6lZYOgd4lzfGmwb3ytbY5lfWOnZDDhdI9xfG:|aYO= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3MUm4PUc,OjJ5NUG5PFk9N2F-
PC-3  M1SxRWFxd3C2b4Ppd{BCe3OjeR?= NVvTN5hYOm2P NFHsTpIzcA>? Mk\4bY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? M1L1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S1OVgxLz5{Mke0OVU5ODxxYU6=
HCT116 M1q1fWFxd3C2b4Ppd{BCe3OjeR?= MVG1cW0> NGLQXFMzPGh? M2fGWWROW09? MlHu[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo M1H2S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK2OVIzLz5{NE[yOlUzOjxxYU6=
U2OS NFG3elZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLQSmsyOG2P MnTkNlRp M1zWSYlvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JGRwgA>? M4nZeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkO5NFE{Lz5{NE[zPVAyOzxxYU6=
A2780cp NWL6OJRvSXCxcITvd4l{KEG|c3H5 NVe1c|U2Oi53bV2= MV:xbC=> Mkju[IRJOk9? MoTR[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> Mn3tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUe5OFYoRjJ2OEG3PVQ3RC:jPh?=
HepG2 NEe0eFhHfW6ldHnvckBCe3OjeR?= NGXLXnE2dU1? NYL2[2plPGh? NETy[YNqdmO{ZXHz[ZMh[2WubIXsZZIhdGW4ZXzzJI9nKEiOwrC= NVjVTpd3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O|E{PTh5PD;hQi=>
Microglia  NXfWVFVWSXCxcITvd4l{KEG|c3H5 M4XaSVVuVQ>? Mn;lNlRp NUOwXo9G\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp NVTZNINTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVg3ODFpPkK0PFE5PjBzPD;hQi=>
MDA-MB 231 MmruRZBweHSxc3nzJGF{e2G7 MUi1cW0> NHLWWI8xNjWq MlzqcY9lfWyjdHXzJHRw[2:vaX9CsuKhcW6mdXPl[EBieG:ydH;zbZM> M1O3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOwO|gyLz5{NEizNFc5OTxxYU6=
PANC-1  NFfmcZFCeG:ydH;zbZMhSXO|YYm= NFHmZoUydU1? MUi0PIg> NYLQZmt7TE2VTx?= NUGwdGo6\W6qYX7j[ZMh[m:{dHX6c41q[i2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? M3eyeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OESyNVU5Lz5{NEi0NlE2QDxxYU6=
MDA-MB-231 NE\GVHZHfW6ldHnvckBCe3OjeR?= MV:ycW0> NVz0e|VKPDiq MX3wdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> NY\tUVZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
MDA-MB-231 MnL2SpVv[3Srb36gRZN{[Xl? NEWzZpozdU1? MYGyOIg> NUfxVIF6cW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF5CpC=> NHnKb3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm3NFY4Pid-MkS5O|A3PzZ:L3G+
MCF-7  MVzGeY5kfGmxbjDBd5NigQ>? M1HHdFJuVQ>? NYroU25mPDiq NFLmUmVxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5ME[3Okc,OjR7N{C2O|Y9N2F-
MCF-7  MXjGeY5kfGmxbjDBd5NigQ>? NXKxZnJEOm2P NEXufG0zPGh? MVzpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi M3XtS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
HepG2 Mmi3RZBweHSxc3nzJGF{e2G7 NVj6Z4FtO22P NUHRTpBOPWh? NV7YPI1PemWmdXPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHFFew>? NHrFUFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizOlU6PSd-MkK4N|Y2QTV:L3G+
HeLa NETUXopCeG:ydH;zbZMhSXO|YYm= MkLXNVBuVQ>? NVnlO3J[Omh? MVXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ NXnFVm5qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFAyOzVpPkKzNFAxOTN3PD;hQi=>
SK-HEP-1 NV;6PJg3SXCxcITvd4l{KEG|c3H5 M3i2fFExdU1? MX[xbC=> M1Tk[5Bzd3SnY4TzJIFo[Wmwc4SgZZV1d3CqYXf5JIFv\CCrbnT1Z4V{KGGyb4D0c5NqeyCrbjDieYZidGmwLYTy[YF1\WRiY3XscJM> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh3OE[0PUc,OjJ6NUi2OFk9N2F-
MDA-MB231 Mmr0SpVv[3Srb36gRZN{[Xl? Mn2zOY1O NWnYVVFDOWh? M3faOIlv[3KnYYPld{Bz\XO4ZYLheJJwdC2vZXTpZZRm\CClYYPwZZNmKGGldHn2ZZRqd25iYX7kJINmdGxiZHXheIg> NYS1TmZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwPFg5PTBpPkKzNFg5QDVyPD;hQi=>
PaCa44 MlO5RZBweHSxc3nzJGF{e2G7 NEDRTIozNjWvTR?= MkPONYg> NFW5fHdz\WS3Y3XzxsBo\W6rcHnuMY1m\GmjdHXkJIFxd3C2b4Ppdy=> M2nQVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUK0NVEzLz5{M{GyOFEyOjxxYU6=
T-47D MXnGeY5kfGmxbjDBd5NigQ>? NVrtW3VuOTCvTR?= MoLjNog> MUTpcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? M1W5elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{CwNFI3Lz5{M{OwNFAzPjxxYU6=
GTL-16  MW\BdI9xfG:|aYOgRZN{[Xl? MUm1cW0> MojuNlRp NFXaeXlz\WS3Y3XzJINmdGxidnnhZoltcXS7IHHzJINwdXCjcnXkJJRwKGOnbHzzJJRz\WG2ZXSge4l1cCCPRWSgbY5pcWKrdH;ydy=> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN|MUO0PVA9N2F-
U251MG  M1[1XmZ2dmO2aX;uJGF{e2G7 M{PpOlNuVQ>? MlvJNYg> NWHp[YFXe3WycILld5NmeyCOQ{OtTWkheHKxdHXpckBmgHC{ZYPzbY9v NFPjZ2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OzPFYyQCd-MkOzN|g3OTh:L3G+
T24 MYPGeY5kfGmxbjDBd5NigQ>? MofSNVBuVQ>? NXX0R5M2OWh? MkfRdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= NV3mS2JnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVQxQDBpPkKzN|U1ODhyPD;hQi=>
HUVECs NVPHVGh6TnWwY4Tpc44hSXO|YYm= NYf5U|lxO22P MlHnNlRp MW\icI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6 NVjT[4dGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVg6OjhpPkKzN|U5QTJ6PD;hQi=>
MCF-7  MUDGeY5kfGmxbjDBd5NigQ>? MnfaOY1O NFz1eJQzPGh? MlXTbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> Mn3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OUW2O|koRjJ|M{m1Olc6RC:jPh?=
Hela  MYjGeY5kfGmxbjDBd5NigQ>? NWLBUXozPW2P MVGyOIg> NF60dIJqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 NF73OWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVY4QSd-MkOzPVU3Pzl:L3G+
OR6 MWHGeY5kfGmxbjDBd5NigQ>? NEDYNm8yOG2P M3W3RlczcA>? Mmryd5VxeHKnc4Pld{BJS1ZicnXwcIlk[XSrb36gZY5lKG[xcn3heIlwdiCxZjDheZRweGijZ3;zc41mew>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NUi3OUc,OjN|OUW4O|U9N2F-
HT-29  NUPpRpRWTnWwY4Tpc44hSXO|YYm= NGTQSHcydU1? M1PTVFQ5Nzl4aB?= M1XJTIlvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= MkXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEiyO|IoRjJ|NUC4NlczRC:jPh?=
SH-SY5Y MkOzR5l1d3SxeHnjbZR6KEG|c3H5 MXG1cW0> MlfqNlRp MYnpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 MnXNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkWyOlUoRjJ|NUK1NlY2RC:jPh?=
Saos-2  Mo\RRZBweHSxc3nzJGF{e2G7 M4i2[lFuVQ>? NG\yUpA6Pmh? NEjVNGdqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IGDDXC=> NFSwS3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N|E4OSd-MkO1OlMyPzF:L3G+
1321N1 NHvZOFNEgXSxdH;4bYNqfHliQYPzZZk> M3raV|VuVQ>? MVeyOIg> M1nZdZBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= NVrreGw1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlUzPjVpPkKzOVI2OjZ3PD;hQi=>
A2780 M1jF[2Fxd3C2b4Ppd{BCe3OjeR?= NYT4bXZEPW2P NXv3V4VTOjSq NULPfJZb[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7MkK4NUc,OjN3OUKyPFE9N2F-
OV2008 M4jL[2Fxd3C2b4Ppd{BCe3OjeR?= MkezOY1O Ml;1NlRp MoX5Z49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? NIfsZ4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5NlI5OSd-MkO1PVIzQDF:L3G+
PC12  MnPvSpVv[3Srb36gRZN{[Xl? MoXYNVBuVQ>? MUiyOIg> NIHFSFZ4[XSnch?= NFTIfInDqGmwaHnibZR{KGOqeX3veJJ6eHOrbj3sbYtmKHC{b4TlZZNwdWGuIHHjeIl3cXS7Lh?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyM{m3PUc,OjN4MEO5O|k9N2F-
SH-SY5Y Ml;XRZBweHSxc3nzJGF{e2G7 Mk\lOY1O NI\qTXQycA>? MlG2ZYJwdGm|aHXzJINmdGG|dILvcEBv\XW{b4Dyc5Rm[3SrdnWg[YZn\WO2 NHfPVW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPVM6PSd-MkO2NVk{QTV:L3G+
SH-SY5Y MmDPSpVv[3Srb36gRZN{[Xl? Ml;JNY1O MUGyOIg> MV7pcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF;DVC=> M1KyRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{SzNVQ5Lz5{M{e0N|E1QDxxYU6=
HepG2 NWDiTHRMTnWwY4Tpc44hSXO|YYm= NXqxPJpEOTCvTR?= M3\oflI1cA>? MofhbY5pcWKrdIOgd4lVUUeDUj2gZY5lKEiEU2OtbY5lfWOnZDDheZRweGijZ4m= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzN{C0NEc,OjN6MUewOFA9N2F-
HeLa MV;GeY5kfGmxbjDBd5NigQ>? M3jQUlExdU1? MojWNog> NGf5eIN{fXCycnXzd4V{KEyFMzDJTUBmgHC{ZYPzbZNwdg>? NEDObHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i2OFc{QCd-MkO4OlQ4Ozh:L3G+
HONE-1  M3TtO2Z2dmO2aX;uJGF{e2G7 M2DwXFVuVQ>? NHTMSpAycA>? NWmxbmFYemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v NX;aNJY{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PVI{PThpPkKzPFkzOzV6PD;hQi=>
MCF7 NHy1TlFHfW6ldHnvckBCe3OjeR?= NEDhRlE2dU1? NXrISHBROjSq Ml;SbY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? MmXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkK2NlkoRjJ|OU[yOlI6RC:jPh?=
HO8910 NIK1W4JCeG:ydH;zbZMhSXO|YYm= MV6xNI1O M{fBRVEzcA>? MULlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> NHPqXnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4N|YyOCd-MkO5PFM3OTB:L3G+
SMMC-7721 NFzCbXhCeG:ydH;zbZMhSXO|YYm= NH21SVE2dU1? MV2yOIg> NFzRe2NifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> NHLxWog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2OlY6Oyd-MkSwOlY3QTN:L3G+
Hep3B NUX1bnZ6SXCxcITvd4l{KEG|c3H5 NX;qRVM6PW2P MYSyOIg> NV7lUnNz[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ NY\k[3RuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl|PD;hQi=>
H460 NIfreGtHfW6ldHnvckBCe3OjeR?= MVWxNI1O NV7SWmRHPGh? NHfPcXJqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M4nn[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUezNlA5Lz5{NEG3N|IxQDxxYU6=
A549 NF7EbVFHfW6ldHnvckBCe3OjeR?= MnTINVBuVQ>? MmfKOIg> MWPpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBqenKjZHnheIlwdg>? NHf0foo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0Nlc{PSd-MkSxOFI4OzV:L3G+
H1299  MonISpVv[3Srb36gRZN{[Xl? MVuxNI1O M2\YelRp NGPHeXJqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NV\FWHNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|MzODhpPkK0NVc{OjB6PD;hQi=>
WiDr MVvGeY5kfGmxbjDBd5NigQ>? MXmxNI1O M3Ti[VFp MVnpcohq[mm2czDQR2JNNWmwZIXj[YQhVEN|IFnJJIV5eHKnc4Ppc44> MoS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOUC0PFkoRjJ2MUmwOFg6RC:jPh?=
LoVo  MmLtRZBweHSxc3nzJGF{e2G7 MYe1cW0> M2n3cFQ5cA>? NF;DOXpmdmijbnPld{BFS0FvaX7keYNm\CCjcH;weI9{cXNw Ml31QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MEG4NVIoRjJ2MkCxPFEzRC:jPh?=
HepG2 E47 M2fyPWZ2dmO2aX;uJGF{e2G7 M1[2XlIvPW2P NV7KeG55PDiq Mm[1bY5kemWjc3XzJJRp\SC2b4jpZ4l1gSCxZjDBRUwhSlORLDDhcoQhS0OuNB?= MmWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{N{O3N|goRjJ2MkezO|M5RC:jPh?=
RKO NWDWcVdITnWwY4Tpc44hSXO|YYm= NV\veYlZOm2P MV6xbC=> NFXLe|VFVVOR MXrlcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= MkHtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUG3O|coRjJ2MkmxO|c4RC:jPh?=
Hep3B MWrBdI9xfG:|aYOgRZN{[Xl? NWCx[pFHOm2P NVTFSphoOTKq NF3oSnFFVVOR MUnpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= MnvvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
ACHN-5968 MUfBdI9xfG:|aYOgRZN{[Xl? MYS1cW0> NUG2[WlWO2h? NHnZV5BmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NXqxdnMyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFU3ODRpPkK0N|A2PjB2PD;hQi=>
Huh7 M3f6UGFxd3C2b4Ppd{BCe3OjeR?= M4DiUFJuVQ>? NWjkcJlTOTKq MoHkSG1UVw>? NXPNeHM3cW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> NH;Tcoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5O|MxOCd-MkSyPVc{ODB:L3G+
UOK257 M3vhd2Fxd3C2b4Ppd{BCe3OjeR?= NEH2dmk2dU1? NHKybpc{cA>? NF;iS|FmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li M1zwclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{C1OlA1Lz5{NEOwOVYxPDxxYU6=
ECSCs  NEe3T5NCeG:ydH;zbZMhSXO|YYm= NV6zdmUyOTCvTR?= NYSxPVd7PGh? NFLqTZZl\WO{ZXHz[ZMhemGyYX35Z4lvNXS{ZXH0[YQh[XCxcITvd4l{ NUXuWIg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVkyODlpPkK0N|E6OTB7PD;hQi=>
MCF-7  M4m1[2Z2dmO2aX;uJGF{e2G7 NFXxZmMyOG2P NYrnSohlOjSq NGj5[lhqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzNUW3PEc,OjR|MUW1O|g9N2F-
SGC-7901  NF7BcnJCeG:ydH;zbZMhSXO|YYm= Mmn5No1O MmrXNYg> NVHFeJpMcW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{MUO0NEc,OjR|MkGzOFA9N2F-
SMMC-7721 MUfBdI9xfG:|aYOgRZN{[Xl? MUGycW0> NYPLZpVCOWh? M3XQWolv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| MlqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkGzOFAoRjJ2M{KxN|QxRC:jPh?=
T24 M3zPS2Fxd3C2b4Ppd{BCe3OjeR?= MYS1cW0> MlLPNU42cA>? NIW4[oNxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? MlHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEmxO|YoRjJ2M{S5NVc3RC:jPh?=
NTUB1 NImyd45CeG:ydH;zbZMhSXO|YYm= NFv5RVU2dU1? NW\QZWd1OS53aB?= NEDmfYVxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? Ml;KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEmxO|YoRjJ2M{S5NVc3RC:jPh?=
MG-63 NHfpRllCeG:ydH;zbZMhSXO|YYm= NXLPWHY3OTCvTR?= NEL4[4EyOmh? M{P4bIVvcGGwY3XzJGRRNWmwZIXj[YQh[XCxcITvd4l{ NW\OVo4zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVg{ODFpPkK0N|U5OzBzPD;hQi=>
MG-63 MWXBdI9xfG:|aYOgRZN{[Xl? MmjjNE42NzGvTR?= MmPJN|Jp NGf5c|lmdmijbnPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygZZBweHSxc3nz NGr4R4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
MG-63 M3;iS2Z2dmO2aX;uJGF{e2G7 Ml7oNE42NzGvTR?= MUm0PIg> NGfhWYVqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MoW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUizOFIoRjJ2M{W4N|QzRC:jPh?=
U2OS  NX3STIQ1TnWwY4Tpc44hSXO|YYm= MnfCNE42NzGvTR?= NIfPTGg1QGh? NUOxc4dKcW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? NHywfHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
HGC-27 MWPGeY5kfGmxbjDBd5NigQ>? NHnVdIgyOG2P M3Xi[VFp NWXnRnJzcW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGyxc4OgZpkhWkGGMECxJI9zKE2NLUKyNFY> M3PlUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEG2N|Q6Lz5{NESxOlM1QTxxYU6=
HCT116  NG\0OoJCeG:ydH;zbZMhSXO|YYm= NWThb2lVPW2P NV7Kcm9ROjSq MUjlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? NYPW[YxVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlY2OjJpPkK0OlI3PTJ{PD;hQi=>
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HepG2 MYjGeY5kfGmxbjDBd5NigQ>? M{e3WVVuVQ>? MWK0bC=> NEi5UGpqdmO{ZXHz[ZMhUExicnXs[YF{\Q>? Mn75QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUO1PFcoRjJ2N{GzOVg4RC:jPh?=
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Nara-H NGXQSWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV[1cW0> MmHvOFhp NXq1fYNw\W6qYX7j[ZMhfGWvc3nyc4xqdXW|bXXkbYF1\WRic4XwdJJme3Orb36gc4YhVmG{YT3IJINmdGxicILvcIln\XKjdHnvci=> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyNUCzN{c,OjF6MEWwN|M9N2F-
HUVECs MYnGeY5kfGmxbjDBd5NigQ>? NX3Ed2ZHOTCvTR?= MkTTNE42cA>? NFfVeVZl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l NYPsXm9ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0Olg2QTJpPkKxOFY5PTl{PD;hQi=>
HepG2 NIX2OlVCeG:ydH;zbZMhSXO|YYm= Ml\qNo1O MV2xbC=> MYTlcohidmOnczDyZYRq[XSrb36tbY5lfWOnZDDj[YxtKGSnYYTo MmXvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NUO2PVEoRjJzNEWzOlkyRC:jPh?=
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A673 MXHxTHRUKGG|c3H5 NHTnZ|NyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY NHfB[IJyUFSVIHHzd4F6 MYDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? M1rMN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 NIj5U3dyUFSVIHHzd4F6 MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| NXe0NGJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 NUC5RZZoeUiWUzDhd5NigQ>? NVXDSWlleUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz M334SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NB-EBc1 NYPFNJl[eUiWUzDhd5NigQ>? Ml3udWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NH3BVW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 MUHxTHRUKGG|c3H5 Mnj3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NIPqU2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 NV;5W3BZeUiWUzDhd5NigQ>? MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? Mle1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65; 

PubMed: 29296191     


Western blotting analysed expression of α-SMA, TGF-β, LC-3B, Beclin-1, NF-κB and NF-κBp65 in hepatic stellate cells.

caspase-3 / caspase-9 / PARP; 

PubMed: 25891159     


EC9706 cell lysates were prepared and subjected to immunoblotting with antibodies against caspase-3, caspase-9 and PARP. The expression ratios in each experiment were determined densitometrically. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; PI, propidum iodide; PARP, poly(ADP ribose) polymerase.

VEGF; 

PubMed: 25891159     


Effects of autophagy inhibition on the protein expression levels of VEGF. Western blot analysis demonstrated that VEGF protein expression levels were decreased when the EC9706 human esophageal squamous cell carcinoma cells were treated with 3-MA. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; VEGF, vascular endothelial growth factor.

APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2; 

PubMed: 26316755     


Presenilin 1, Nicastrin, and Pen-2 were increased with the 3-MA treatment (*P<0.001) while the other component of the γ-secretase complex (APH-1 and Presenilin 2) has no difference. 3-MA, 3-methyladenine; Con, control; APP, amyloid protein precursor.

29296191 25891159 26316755
Immunofluorescence
LC3 / Hif-α / COX2; 

PubMed: 29039446     


Chemical interventions of autophagy and ischemic-hypoxic injury, assessed using immunochemical double labeling. Compared with the control group, expression levels of HIF-α and COX2 were altered by chemical intervention. (A) Chemical intervention altered the expression of HIF-α and autophagosmes. Following autophagy antagonist (3-MA) intervention, expression of HIF-α was enhanced, compared with that in the OGD group, and was decreased by the autophagy agonist, rapamycin. In the MHY treatment group, expression of HIF-α was similar to that in the 3-MA group. (B) Chemical intervention showed changes in the expression of COX2 and autophagosomes. Changes in the expression of COX2 were similar to changes in HIF-α. Scale bar, 30 µm. OGD, oxygen-glucose deprivation; HIF-α, hypoxia-inducible factor-α; COX2, cyclooxygenase 2; 3-MA, 3-methyladenine; RP, rapamycin; MHY, MHY1485.

29039446
Growth inhibition assay
Cell viability; 

PubMed: 26934124     


KYSE 180 cells were co-treated with EDHB (40 μg/ml) and various concentrations of 3-MA for 24 h (left) or 48 h (right), and cell viability was determined by WST-8 assays.

26934124
体内研究 3-Methyladenine (3-MA)可以通过对磷酸肌醇3磷酸激酶(PI3K) 的作用来阻断自吞噬, 而PI3K的活性对于自噬体形成早期膜池的成核和组装是必须的。与SAH 处理组相比3-MA并不会改变出血的程度。与SAH +对照成分组相比经过3-MA预处理后会明显加重神经病学症状。3-MA处理会减少自吞噬发生[5]。 相反地,在SAH + 3-MA组里 断裂的半胱天冬酶表达量明显上调,与此一致的是与SAH + 对照成分组相比,SAH + 3-MA组中右脑皮层里原位末端标记阳性细胞数量明显增多[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[4]
- 合并

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
细胞实验:[2]
- 合并
  • Cell lines: HeLa 细胞系
  • Concentrations: 1-10 mM
  • Incubation Time: 3 分钟
  • Method: 用台盼蓝染色法分析细胞存活率(如HeLa细胞)。简言之, 经过3-MA处理后将贴壁的与漂浮的细胞都用PBS( pH 7.4)重悬,密度控制在每毫升1-2×106。加入等体积的0.4% 台盼蓝并彻底混匀。室温孵育3分钟后用血细胞计数器对细胞计数分析。
    (Only for Reference)
动物实验:[5]
- 合并
  • Animal Models: 体重 300-350 g 的成年雄性Sprague–Dawley 大鼠
  • Dosages: 400 nM
  • Administration: 脑室给药
    (Only for Reference)

溶解度 (25°C)

体外 Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 149.15
化学式

C6H7N5

CAS号 5142-23-4
储存条件 3年 -20°C 粉状
别名 NSC 66389

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2