Pictilisib (GDC-0941)

For research use only. Not for use in humans.

目录号：S1065 别名： RG7321

Pictilisib (GDC-0941) Chemical Structure

CAS No. 957054-30-7

Pictilisib (GDC-0941, RG7321) 是一种有效的PI3Kα/δ抑制剂，在无细胞试验中IC50为 3 nM，对p110β (11倍)和p110γ (25倍)具有适度的选择性。Pictilisib (GDC-0941) 可诱导自噬和凋亡。Phase 2。

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10mM (1mL in DMSO)	RMB 1972.48	现货
5mg	RMB 986.88	现货
10mg	RMB 1390.74	现货
50mg	RMB 3026.17	现货
200mg	RMB 7926.11	现货
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客户使用Selleck生产的Pictilisib (GDC-0941)发表文献339篇：

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Harvard University, 2014,

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Blood, 2013, 121(12):2274-84

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产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述

靶点

p110α ^[1] (Cell-free assay)	p110δ ^[1] (Cell-free assay)	p110β ^[1] (Cell-free assay)	p110γ ^[1] (Cell-free assay)	mTOR ^[1] (Cell-free assay)
3 nM	3 nM	33 nM	75 nM	0.58 μM(Ki app)

体外研究

GDC-0941也等效作用于PI3Kα和 PI3Kδ和PI3Kα突变型E545-K和H1047-R,也选择性作用于PI3Kβ(10倍)和 PI3Kγ(25倍), 选择性更高地作用于PI3K II, III, 和IV类成员，包括, C2β, Vps34, DNA-PK和mTOR。GDC-0941 作用于U87MG, PC3, 和 MDA-MB-361 细胞，有效抑制Akt磷酸化，IC50分别为46 nM, 37 nM,和 28 nM。GDC-0941抑制U87MG, A2780, PC3, 和MDA-MB-361细胞增殖，IC50分别为 0.95 μM, 0.14 μM, 0.28 μM, 和0.72 μM。^[1]GDC-0941处理，有效抑制对Trastuzumab敏感和不敏感的HER2扩增细胞增殖，IC50为149-944 nM,与 Trastuzumab敏感性无关。GDC-0941抑制含PIK3CA突变的HER2扩增的细胞增殖，IC50<500 nM, 且有效抑制抗trastuzumab 的HER2 扩增乳腺癌细胞增殖和活力。^[2]GDC-0941 显著抑制HCT116, DLD1和 HT29细胞生长，GI50分别为1081 nM, 1070 nM 和157 nM。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
Sf9	M3zmXGtqdmG\|ZTDBd5NigQ>?		NHS2coMyKGh?	NH7INmJFVVOR	MljCTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCSSUPLJJAyOTCmZXz0ZUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy\|IHPv[ZhxemW\|c3nu[{BxQDWjbIDoZUB4cXSqIFnDOVAhd2ZiMD6wNFMh\|ryP	M4f4UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
Sf9	NFfKUJZMcW6jc3WgRZN{[Xl?		MlLtNUBp	MoSySG1UVw>?	MkixTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCSSUPLJJAyOTCkZYThJGgyODR5LWKgcZV1[W62IHX4dJJme3OnZDDpckBU\jliY3XscJMh[2:neIDy[ZN{cW6pIIC4OYFteGijIIfpeIghUUN3MDDv[kAxNjByMzFOwG0>	M{[5U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
Sf9	MmLXT4lv[XOnIFHzd4F6		Mlm3NUBp	M3\wcWROW09?	NX\GWYUxUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDQTVNMKHBzMUDi[ZRiKEV3NEWtT{BufXSjboSg[ZhxemW\|c3XkJIlvKFOoOTDj[YxteyClb3X4dJJme3OrbnegdFg2[WyyaHGge4l1cCCLQ{WwJI9nKDBwMECzJO69VQ>?	NX;PbGpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3OVQ3PTRpPkG4O\|U1PjV2PD;hQi=>
Sf9	NGeyWnBMcW6jc3WgRZN{[Xl?		MXuxJIg>	M4nYTWROW09?	MlzoTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCSSUPLJJAyOTCkZYThJIV5eHKnc4Pl[EBqdiCVZkmgZ4VtdHNiY3;lfJBz\XO\|aX7nJJA5PWGucHjhJJdqfGhiSVO1NEBw\iByLkCzN{DPxE1?	NV;tZWJHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3OVQ3PTRpPkG4O\|U1PjV2PD;hQi=>
Sf9	MkfzT4lv[XOnIFHzd4F6		MmXqNUBp	M4HQTGROW09?	NX24VHZnUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDQTVNMKHBzMUDhcJBp[SCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJINw\XiycnXzd4lv\yCyOEXhcJBp[Q>?	M1q5WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
MDA-MB-361	NGHLXHhHfW6ldHnvckBCe3OjeR?=		Mn\SNkBp	MnPqSG1UVw>?	NIHSVWNKdmirYnn0bY9vKG:oIFHreEBU\XJ2N{OgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIF3ERU1OSi1\|NkGgZ4VtdHNid3n0bEBRUUt\|Q1GgSVU1PS2NIH31eIF1cW:wIIfpeIghUUN3MDDv[kAxNjB{ODFOwG0>	M1f5WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
PC3	NUn3WGYxTnWwY4Tpc44hSXO\|YYm=		MX6yJIg>	MV3EUXNQ	M4jncGlvcGmkaYTpc44hd2ZiQXv0JHNmejR5MzDwbI9{eGixconsZZRqd25iaX6gVHRGVi2wdXzsJIh2dWGwIGDDN{Bk\WyuczD3bZRpKEmFNUCgc4YhOC5yM{eg{txO	M3m1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
U87MG	NIPrR3BHfW6ldHnvckBCe3OjeR?=		NF;zRlQzKGh?	MUHEUXNQ	MlPQTY5pcWKrdHnvckBw\iCDa4SgV4VzPDd\|IIDoc5NxcG:{eXzheIlwdiCrbjDQWGVPNW63bHygbJVu[W5iVUi3UWch[2WubIOge4l1cCCLQ{WwJI9nKDBwMES2JO69VQ>?	NHLrT4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe1OFY2PCd-MUi3OVQ3PTR:L3G+
MDA-MB-361	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NIC2U\|c6PiCq	MXvEUXNQ	NHH2fGJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1\|NkGgZ4VtdHNid3n0bEBRUUt\|Q1GgSVU1PS2NIH31eIF1cW:wIIfpeIghUUN3MDDv[kAxNjd{IN88US=>	NWn0WpRvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3OVQ3PTRpPkG4O\|U1PjV2PD;hQi=>
A2780	MnX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NIXCc\|g6PiCq	NIHvdldFVVOR	NHvURotCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGyO\|gxKGOnbHzzJJdqfGhiUFnLN2NCKGGwZDDQWGVPKG23dHH0bY9vKHerdHigTWM2OCCxZjCwMlE1KM7:TR?=	MkfNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5NUS2OVQoRjF6N{W0OlU1RC:jPh?=
PC3	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M17pWFk3KGh?	MXTEUXNQ	M4TmbGlEPTB;MD6yPEDPxE1?	Mle4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5NUS2OVQoRjF6N{W0OlU1RC:jPh?=
U87MG	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M1mxdVk3KGh?	NXnWSW9oTE2VTx?=	M1f5fmlEPTB;MD65OUDPxE1?	M1\S[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{W0OlU1Lz5zOEe1OFY2PDxxYU6=
Sf9	M4\UW2tqdmG\|ZTDBd5NigQ>?		MnjWN\|AhdWmw	MoPUSG1UVw>?	NI\vc2hKdmirYnn0bY9vKG:oIHj1cYFvKFCLM1vnZY1u[SCneIDy[ZN{\WRiYYPz[ZN{\WRiYYOgZY1wfW62IH;mJGFVWCClb37zeY1m\CC5aYToJGlEPTBib3[gNE4xPDJizszN	NHXMe2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0PFM1Oid-MkK1OFg{PDJ:L3G+
RAW 264.7	MnvqT4lv[XOnIFHzd4F6		NHvEN4g6OCCvaX6=	M4L2dWROW09?	Mm\QTY5pcWKrdHnvckBw\iCqdX3hckBRUTONZ3HtcYEh\XiycnXzd4VlKGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhSUuWIIDoc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4zQThizszN	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2OEO0Nkc,OjJ3NEizOFI9N2F-
Sf9	M1nIbGtqdmG\|ZTDBd5NigQ>?		NU\XUHVXOSCq		NHTzb3JKdmirYnn0bY9vKG:oIFfTWE1nfXOnZDDoeY1idiC{ZXPvcYJqdmGwdDDQTVNM[mW2YTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiU1[5JINmdGy\|IIfpeIghUUN3MDDv[kAxNjBzNzFOwG0>	MoDuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEC2OFUoRjJ\|NUSwOlQ2RC:jPh?=
Sf9	MVnLbY5ie2ViQYPzZZk>		M1fFS\|EhcA>?		MlfnTY5pcWKrdHnvckBw\iCJU2St[pV{\WRiaIXtZY4hemWlb33ibY5idnRiUFmzT4RmdHSjIHX4dJJme3OnZB?=	NXPLUYhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFA3PDVpPkKzOVQxPjR3PD;hQi=>
Sf9	MofTT4lv[XOnIFHzd4F6		NWDMSWl{OSCq		MorWTY5pcWKrdHnvckBw\iCJU2St[pV{\WRiaIXtZY4hemWlb33ibY5idnRiUFmzT4didW2jIHX4dJJme3OnZB?=	NVOxRWhvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFA3PDVpPkKzOVQxPjR3PD;hQi=>
Sf9	M1ntO2tqdmG\|ZTDBd5NigQ>?		Ml7TNUBp		M{DVZ2lvcGmkaYTpc44hd2ZiR2PUMYZ2e2WmIHj1cYFvKHKnY3;tZolv[W62IGDJN2tidHCqYTDlfJBz\XO\|ZXS=	NWjTWGpnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFA3PDVpPkKzOVQxPjR3PD;hQi=>
SKOV3	M3TGZmtqdmG\|ZTDBd5NigQ>?	NHS5ZpEyKM7:TR?=	MVmxJIg>	MWfEUXNQ	M{PrTWlvcGmkaYTpc44hd2ZiUFmzT4FteGijIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQXv0JHNmejR5MzDwbI9{eGixconsZZRqd25?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4MEO0PEc,OjN\|NkCzOFg9N2F-
MCF7	NFLBWFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NX\pZnBTOSEQvF2=	MlTxPVYhcA>?	NEfxdIZFVVOR	MUfHTVUxRTBwMEewNkDPxE1?	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4MEO0PEc,OjN\|NkCzOFg9N2F-
SKOV3	M3SyVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFfZ[48yKM7:TR?=	NUGye5l7QTZiaB?=	MkL2SG1UVw>?	M17GS2dKPTB;MD6xOFc3KM7:TR?=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4MEO0PEc,OjN\|NkCzOFg9N2F-
PC3	NVr1UJN5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NVXielJ1PzJiaB?=	MYnEUXNQ	M{DDUWlEPTB;MD6yPEDPxE1?	Ml\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OEG3NVQoRjJzOUixO\|E1RC:jPh?=
MCF7.1	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MnPwO\|IhcA>?	MnLKSG1UVw>?	M17xTWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{4yKGOnbHzzJIV5eHKnc4PpcochUEWUMjDn[Y5mKHerdHigTWM2OCCxZjCwMlI5KM7:TR?=	NUDCNpJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PFE4OTRpPkKxPVgyPzF2PD;hQi=>
MCF7-neo/Her2	NHTXNIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NVfKO2xmPzJiaB?=	MX7EUXNQ	NV7DO3B6UUN3ME2wMlI6KM7:TR?=	MljxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NkK5NFMoRjJ\|Nk[yPVA{RC:jPh?=
MCF7-neo/Her2	M13sXmtqdmG\|ZTDBd5NigQ>?				NInTN\|FKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiQVvUJJBpd3OyaH;yfYxifGmxbjDheEBUPDd\|IIfpeIghUUN3MDDv[kAxNjByNzFOwG0>	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ4MkmwN{c,OjN4NkK5NFM9N2F-
PC3	NIDYWGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MYC3NkBp	MUDEUXNQ	NV3EbIZUTUN3ME2wMlM1KM7:TR?=	M4TFfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUi1OlM6Lz5{MUm4OVY{QTxxYU6=
MCF7.1	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MYS3NkBp	MnW2SG1UVw>?	NUTjepVDSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4NjFiY3XscJMh\XiycnXzd4lv\yCSSUPLZYxxcGFibYX0ZY51KHerdHigSWM2OCCxZjCwMlM6KM7:TR?=	MoryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OEW2N\|koRjJzOUi1OlM6RC:jPh?=
PC3	MmXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NF:5bHM4OiCq	NIGzd\|ZFVVOR	MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCmZX\pZ4lmdnRiaX6gVHRGViC5aYToJGlEPTBib3[gNE4{QSEQvF2=	MoT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNUC2OlkoRjJyMEWwOlY6RC:jPh?=
MCF7.1	NVXsPWpsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NWHjN2wyPzJiaB?=	M1S0XmROW09?	NV;ZRoVGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4NjFiY3XscJMh\XiycnXzd4lv\yCSSUPLZYxxcGFibYX0ZY51KHerdHigTWM2OCCxZjCwMlM1KM7:TR?=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB3ME[2PUc,OjByNUC2Olk9N2F-
MCF7.1	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M3S1OlczKGh?		NHjVN\|BGSzVyPUCuO\|Ih\|ryP	NXfmeVl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOFY3PTZpPkKwN\|Q3PjV4PD;hQi=>
NCI-H1975	NVPSWFR6S3m2b4TvfIlkKEG\|c3H5		NYTt[\|NFPzJiaB?=		NX\3[mZRUUN3ME2wMlI4KM7:TR?=	MnrXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNUm1OFUoRjJ\|MEW5OVQ2RC:jPh?=
H460	MkKxR5l1d3SxeHnjJGF{e2G7		NULTe\|FyPzJiaB?=		M1Lp[WlEPTB;MD64O{DPxE1?	NFXyUZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1PVU1PSd-MkOwOVk2PDV:L3G+
HT-29	NVHVdVNqS3m2b4TvfIlkKEG\|c3H5		MlzSO\|IhcA>?		M{nwUGlEPTB;MD62OkDPxE1?	NHXqOFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1PVU1PSd-MkOwOVk2PDV:L3G+
HepG2	MmT0R5l1d3SxeHnjJGF{e2G7		M17tWlczKGh?		MlvITWM2OD1zIN88US=>	M1PNSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEW5OVQ2Lz5{M{C1PVU1PTxxYU6=
SGC7901	MmC0R5l1d3SxeHnjJGF{e2G7		NFTLNJA4OiCq		M2fsXWlEPTB;MT64JO69VQ>?	MljnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNUm1OFUoRjJ\|MEW5OVQ2RC:jPh?=
NCI-H226	M2rHO2N6fG:2b4jpZ{BCe3OjeR?=		MX23NkBp		MmHjTWM2OD1{Lkmg{txO	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB3OUW0OUc,OjNyNUm1OFU9N2F-
A549	NEPMTnFEgXSxdH;4bYMhSXO\|YYm=		M{j4W\|czKGh?		M3rnO2lEPTB;Nj65JO69VQ>?	M4\LPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEW5OVQ2Lz5{M{C1PVU1PTxxYU6=
U87MG	NETpRndEgXSxdH;4bYMhSXO\|YYm=		M{juTlczKGh?		NGXXU3BKSzVyPUeuO\|ch\|ryP	M{H6cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEW5OVQ2Lz5{M{C1PVU1PTxxYU6=
HBC4	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MULsc4dIUTVyPT22MlA{KE1?	NXTvd3NLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
BSY1	NV3xbFY4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mmr0cI9oT0l3ME2tOk46OCCP	NFXiS3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
HBC5	NFP1emtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MonIcI9oT0l3ME2tOk43OCCP	NF;IVJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MCF7	M4rzWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoT6cI9oT0l3ME2tO{4{OyCP	MlnSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
MDA-MB-231	NHnHe\|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NX;rfY5YdG:pR1m1NF0uPS52NjDN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
U251	M3uzc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoTWcI9oT0l3ME2tOU42OCCP	Mn\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
SF268	NX;zOINRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NViwfGxSdG:pR1m1NF0uPS5\|OTDN	NUTCcIRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
SF295	Mn7JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmjucI9oT0l3ME2tOk4{PyCP	NGTMfo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
SF539	M1z5N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmfYcI9oT0l3ME2tOk42OCCP	NHPFTVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
SNB75	MkjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF7Bc49td2eJSUWwQU04NjFyIF2=	M{jETVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
SNB78	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1H6coxw\0eLNUC9MVYvPTJiTR?=	NVrwVYxsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
HCC2998	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NEPodY5td2eJSUWwQU03NjJ7IF2=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
KM12	MoPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWHsc4dIUTVyPT21Mlc3KE1?	NGXZeYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
HT-29	M3zIR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVvsc4dIUTVyPT22MlMxKE1?	NI\YNWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
HCT15	NXTGdIQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlXVcI9oT0l3ME2tOk4yOiCP	M1P0TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
HCT116	NYLqTpd{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{HGc4xw\0eLNUC9MVUvQDdiTR?=	NYnENVhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
NCI-H23	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXfq[2U{dG:pR1m1NF0uPS55MzDN	NVvhcY5yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
NCI-H226	MoWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmfocI9oT0l3ME2tOk4zOSCP	NHT4So49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
NCI-H522	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4i0OYxw\0eLNUC9MVYvPThiTR?=	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
NCI-H483	NFjCTppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFnscVdtd2eJSUWwQU03NjV4IF2=	NVXTOWlTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
A549	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmTScI9oT0l3ME2tOk4yPSCP	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
DMS273	MoO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXnQbpF4dG:pR1m1NF0uPi52MzDN	NEfnUFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
DMS114	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYLsc4dIUTVyPT21MlkzKE1?	MnrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
LOXIMVI	NEnCN4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmnwcI9oT0l3ME2tOk4zQSCP	NEf3[5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
OVCAR3	MnjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlLncI9oT0l3ME2tOk44OSCP	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
OVCAR4	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYXsc4dIUTVyPT21Mlc3KE1?	NV;X[Vd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
OVCAR5	MoXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NIPQXXRtd2eJSUWwQU03NjJ4IF2=	NWj6c4dpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
OVCAR8	NH;nVlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Mn\acI9oT0l3ME2tOU44OyCP	NGXSPIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
SKOV3	Mny3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3PrbYxw\0eLNUC9MVYvPTJiTR?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
RXF631L	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUDsc4dIUTVyPT22Mlg5KE1?	M3m1OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
ACHN	NE\LZmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M17xNoxw\0eLNUC9MVYvODdiTR?=	MmXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
St-4	NH7afJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnnzcI9oT0l3ME2tOU42PiCP	NUfKdJVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
MKN1	NYrCZmRxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M32zZoxw\0eLNUC9MVcvODhiTR?=	NIjYfYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MKN7	MkP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MX3sc4dIUTVyPT22MlUxKE1?	MnrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
MKN28	NGi1SZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkjOcI9oT0l3ME2tOU42OiCP	M1v5SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
MKN45	NIrWOFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NXraZ\|ZLdG:pR1m1NF0uPi53NTDN	M3PTTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
MKN74	NGPqT\|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVnhOHFmdG:pR1m1NF0uPi5yMjDN	NULCVWhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
DU145	M3qy[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVz2b3ExdG:pR1m1NF0uPS56NTDN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
PC3	MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2PBdYxw\0eLNUC9MVYvPjZiTR?=	M4nwVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
ES5	NHXWRWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUHJR\|UxRTVyLkKxJI5O	M3XxVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
HH	NIHkdGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVP0[Y1yUUN3ME2xNVEvODhibl2=	M4m5[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
MHH-PREB-1	M1rvSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFLVZndKSzVyPUGxOE4{KG6P	M1jXfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
NCI-H1770	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NIK0elBKSzVyPUGyN{45PiCwTR?=	NXHzVVRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
HuO9	NVvpU\|dxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NYDsNZVbUUN3ME2yNFYvODJibl2=	Mm[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
HAL-01	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnmxTWM2OD1{ME[uO\|Mhdk1?	NXXhS\|ZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
NOS-1	M{nBTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFO3O3BKSzVyPUKxNU45OiCwTR?=	Mk\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
HGC-27	NXLyO\|llT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHP1OYVKSzVyPUKxNk42PyCwTR?=	MnjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
NB69	NVrPfo5{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlfkTWM2OD1{NEWuNlchdk1?	MnrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
RS4-11	NV7se2NjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXTJR\|UxRTJ4MT63OkBvVQ>?	M4n4S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
DOK	M4XYS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWjJR\|UxRTNyMT63JI5O	NYHLS4FCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
LB2241-RCC	NXizdJVST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFPhXW5KSzVyPUOwN{45PCCwTR?=	Mmn5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
NCI-H1623	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnjsTWM2OD1\|MESuNFghdk1?	M{jQ[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
YH-13	MnPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYPFdotFUUN3ME2zNFQvOzJibl2=	NIP0[oo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OjF6NUGvK\|5USU6JRWK8M4E,
BPH-1	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUPJR\|UxRTNzNDDuUS=>	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
AN3-CA	NXSzbnV{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWXWVGVzUUN3ME2zOFAvOzdibl2=	MmHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
ES7	M4LQOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlnvTWM2OD1\|NEGuPVkhdk1?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
MDA-MB-175-VII	NXTlS2NXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGjYWo5KSzVyPUO1Nk46OiCwTR?=	NV7Rc5ExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
GAMG	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnvMTWM2OD1\|N{CuPFUhdk1?	NYP2XIMyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
CHL-1	NIjnNJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1zZXmlEPTB;M{e4MlY2KG6P	M4rSdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
NB7	MkLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mk\kTWM2OD1\|OEmuPFMhdk1?	M{f2U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
MFE-280	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlzTTWM2OD12MUiuNkBvVQ>?	MnzUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
HL-60	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlLDTWM2OD12MkWuO\|Uhdk1?	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
GCIY	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4HESmlEPTB;NEOzMlghdk1?	M1flTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
MDA-MB-361	NETsd4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYXJR\|UxRTR\|OT6xN{BvVQ>?	NWHqeoJVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
LXF-289	NI\scW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmL6TWM2OD12NEiuNlchdk1?	M2X3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
NBsusSR	NULZenRbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4rT[mlEPTB;NEi4Mlg2KG6P	NXHVN5J3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
KU-19-19	NGfYVJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Mn;xTWM2OD13MU[uNFchdk1?	MlLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
A204	MkXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MY\JR\|UxRTV{Mj6zN{BvVQ>?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
LB1047-RCC	M4CxNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NF25UXNKSzVyPUWyNk46OyCwTR?=	M3nOeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
COLO-684	M4j2d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NHLBPVJKSzVyPUWyN{4yOSCwTR?=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
PA-1	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkXSTWM2OD13M{KuOFEhdk1?	NUPVV4pFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
MG-63	NEP3b2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGPRdopKSzVyPUW0NU4yOSCwTR?=	NGrwS3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OjF6NUGvK\|5USU6JRWK8M4E,
BHT-101	NFToVlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1r1UWlEPTB;NUS0Mlk1KG6P	NFjqTlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OjF6NUGvK\|5USU6JRWK8M4E,
NKM-1	M33TfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXfJR\|UxRTV7Mz6wPUBvVQ>?	M4j4OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
T47D	NIjtSFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NV3iVnNXUUN3ME21PVcvPTZibl2=	MkDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
MOLT-4	M3rmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Ml7zTWM2OD14MkOuOlIhdk1?	NV\hO3huRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
IGROV-1	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmLsTWM2OD14M{SuN\|Ehdk1?	NFvifJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OjF6NUGvK\|5USU6JRWK8M4E,
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CAMA-1	M3S2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3rqXmlEPTB;M{iuPVY6OSEQvF2=	NUnWbo9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
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SK-MEL-24	NG\NNZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGXXenhKSzVyPUSwMlU6OzFizszN	NXy3NpJkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
KYSE-140	NIjrd5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX7JR\|UxRTRyLkexOlUh\|ryP	NYe1TJhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
MOLT-16	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFzxVZJKSzVyPUSxMlE2OzZizszN	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
CFPAC-1	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlLYTWM2OD12Mj61NlEyKM7:TR?=	MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
NB14	M2XiWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnTxTWM2OD12Mj63NFI4KM7:TR?=	M17rZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
COLO-792	M4jhTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWnJR\|UxRTR{Lki5OlEh\|ryP	NX7LdG16RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
RH-1	NEnWWnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4D5[2lEPTB;NEOuOFg{PiEQvF2=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NlE5PTFxJ{7TRW5ITVJ:L3G+
NCI-H522	MlXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M{TXbmlEPTB;NESuN\|U3QCEQvF2=	NX;LcXNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
Ca-Ski	NH;6NZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUnW[HJGUUN3ME20OE45OTF2IN88US=>	MnrOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
KGN	M1fGZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUL3fIRCUUN3ME20PE4{ODZ\|IN88US=>	M4e0[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzOTh3MT:nQnNCVkeHUkyvZV4>
UACC-62	M1;BR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Ml\WTWM2OD12OD60NFczKM7:TR?=	NUTqUGhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
NCI-H358	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NW[xXWp7UUN3ME20PU4{ODF6IN88US=>	MnniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVIyQDVzLze+V2FPT0WUPD;hQi=>
LS-411N	NYG4dXd2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NILmNlBKSzVyPUS5MlMyOThizszN	NWrjPW81RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTJzOEWxM{c,W0GQR1XSQE9iRg>?
IST-MES1	MkPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3nYUWlEPTB;NEmuOVYzPSEQvF2=	NGq5Too9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OjF6NUGvK\|5USU6JRWK8M4E,

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Assay

Methods	Test Index	PMID
Western blot	p-AKT / AKT / p-FoxO3a / FoxO3a / PUMA; PubMed: 29137323 Oncotarget HCT116 cells were treated with 4μmol/L NVP-BKM120 or 4μmol/L Pictilisib for 24 hours. Indicated protein levels were analyzed by Western blotting. p-S6RP / S6RP; PubMed: 30042442 Cell Death Dis Western blot analysis of proteins as indicated in 4T1 cells treated with 1 µM GDC-0941 or vehicle for 24 h. Vinculin was used as a loading control. p-4EBP1 / 4EBP1; PubMed: 25857298 Oncotarget Cells were treated with 0.5 μM GDC-0941 for the indicated time course and then collected for Western blot analysis of p-4EBP1. p-LRP6 / LRP6; PubMed: 25857298 Oncotarget MDAMB-231, HCC1937 and MCF7 cells were treated with 0.5 µM GDC-0941 for 1 hr, and cell lysates were used for immunoblot analyses. p-PAK / PAK1 / p-CRAF / RAF / p-MEK / MEK / p-ERK / ERK; PubMed: 24327733 Proc Natl Acad Sci U S A Cells were treated with 1 μM GDC-0941 for the indicated times, and lysates were probed with the indicated antibodies. Independent experiments were performed at least three times, and a representative result is shown.	29137323 30042442 25857298 24327733
Immunofluorescence	p65; PubMed: 30042442 Cell Death Dis Representative images of immunofluorescent staining of p65 cellular localization in 4T1 cells treated with 1 µM GDC-0941 or vehicle for 24 h. 4T1 cells were cultured alone or co-cultured with RAW264.7 macrophages. Representative images of three independent experiments are shown. beta-catenin; PubMed: 25857298 Oncotarget Cells were seeded on coverslips the day before the experiment and treated with 0.5 µM GDC-0941 for 18 hr. The immunofluorescence (IF) of beta-catenin is indicated as red fluorescence, and double staining with DAPI is blue. Magnification: 400 X for the left three panels. The right panel shows enlarged images from the white squares.	30042442 25857298
Growth inhibition assay	Cell viability; PubMed: 27117832 J Thorac Oncol The viability of the indicated cells treated with increasing concentrations of GDC-0941 (ranging from 70nM to 20 μM) for 72 hours, determined by CellTiter Glo Viability assay. The IC50 concentrations were calculated using GraphPad Prism program. The assay was performed in triplicate, and the error bars represent standard deviations.	27117832

体内研究

在微粒体中，GDC-0941仅有有限的代谢，其口服生物利用度为78%^[5]。GDC-0941 每天按 75 mg/kg剂量处理携带人U87MG 恶性胶质移植瘤的雌性NCr无胸腺小鼠，显著抑制生长，肿瘤生长抑制率为 83%。^[1]GDC-0941 每天按 150 mg/kg剂量口服处理携带HER2扩增的抗Trastuzumab的MDA-MB-361.1移植瘤的小鼠，抑制肿瘤生长，且显著延迟肿瘤衰退，与肿瘤中强诱导型凋亡相关。^[2] GDC-0941 每天按75 mg/kg剂量处理携带自发性B细胞滤泡性淋巴瘤的PTEN^+/-LKB1^+/hypo小鼠，持续2周，诱导肿瘤体积下降~40%，伴随着AKT, S6K 和SGK(血清和糖皮质激素蛋白激酶)蛋白激酶的磷酸化被切除。GDC-0941抑制肿瘤细胞增殖，诱导凋亡和抑制中心母细胞数。^[3]

激酶实验： ^[1]	- 合并闪烁接近检测: 使用p85α调节亚基，使重组人PI3Kα, PI3Kβ, 和PI3Kδ在 Sf9 杆状病毒系统中共表达，在谷胱甘肽-琼脂糖凝胶上进行亲和层析而纯化GST-融合蛋白。重组人 PI3Kγ表达作为单体 GST融合，也想上述相似方法纯化。GDC-0941 溶于DMSO，加到含200 μg硅酸钇(Ysi) 聚赖氨酸SPA 磁珠, 4 mM MgCl₂, 1 mM 二硫苏糖醇 (DTT), 1 μM ATP, 0.125 μCi [γ-³³P]-ATP, 和4% (v/v) DMSO的20 mM Tris-HCl (pH 7.5)上，总体积为 50 μL。PI3Kα (5 ng), PI3Kβ(5 ng), PI3Kδ(5 ng), 或PI3Kγ(5 ng)的重组GST-融合，加到实验混合物中，开始激酶反应。在室温下温育1小时后, 加入150 μL PBS终止反应。混合物在2000 rpm 转速下离心2分钟，然后使用Wallac Microbeta计数器读数。在 MDL Assay Explorer系统中使用S型，剂量-反应曲线，计算进行至少2次独立重复实验，取平均值，作为IC50值。
细胞实验： ^[2]	- 合并 Cell lines: SKBR-3, BT474-M1, AU-565, HCC-1419, ZR75-30, KPL-4, JIMT-1, BT474-EEI, HCC-1954, MCF-7, CALU-3, SKOV-3, 和MKN-7 Concentrations: 溶于DMSO,终浓度为~10 μM Incubation Time: 48,和72小时 Method: 使用不同浓度 GDC-0941处理细胞48,和 72小时。通过CellTiter-Glo Luminescent细胞活性检测实验测定细胞增殖/活力。通过Western Blot分析pAKT (Ser473), cleaved caspase-3, 和cleaved PARP通过 Caspase-Glo 3/7实验和细胞死亡检测 ELISAplus 实验分别测定caspase 3/7活性, 和凋亡。 (Only for Reference)
动物实验： ^[2]	- 合并 Animal Models: 移植MDA-MB-361.1细胞的NCR裸鼠, 皮下移植BT474-M1 细胞的SCID, C.B-17/IcrHsd-Prkdc^scid 小鼠 Dosages: ~150 mg/kg/day Administration: 口服饲喂 (Only for Reference)

参考文献

[4] García-Martínez JM, et al. Br J Cancer, 2011, 104(7), 1116-1

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