Wortmannin

目录号:S2758 别名: KY 12420

Wortmannin Chemical Structure

Molecular Weight(MW): 428.43

Wortmannin是一种首次命名的PI3K抑制剂,在无细胞试验中IC50为3 nM,对 PI3K 家族的选择性较低。也会抑制自噬体的形成,并有效抑制DNA-PK/ATM,在无细胞试验中IC50为16 nM和150 nM。

规格 价格 库存 购买数量  
RMB 899.84 现货
RMB 1414.71 现货
RMB 2210.53 现货
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产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 Wortmannin是一种首次命名的PI3K抑制剂,在无细胞试验中IC50为3 nM,对 PI3K 家族的选择性较低。也会抑制自噬体的形成,并有效抑制DNA-PK/ATM,在无细胞试验中IC50为16 nM和150 nM。
靶点
PI3K [3]
(Cell-free assay)
DNA-PK [12]
(Cell-free assay)
ATM [12]
(Cell-free assay)
MLCK [1]
(Cell-free assay)
3 nM 16 nM 150 nM 170 nM
体外研究

钙或肽底物不影响Wortmannin对MLCK的抑制,但高浓度的ATP会减弱此种抑制。Wortmannin直接与MLCK催化结构域相互作用,导致不可逆转的酶活性减弱。Wortmannin对cAMP依赖蛋白激酶,cGMP依赖的蛋白激酶,钙调蛋白依赖激酶II,和蛋白激酶C均无抑制作用。[1] Wortmannin抑制由fMLP诱导的PtdInsP3(磷脂酰肌醇3,4,5-三磷酸化)形成,IC 50为5 nM 。在人中性粒细胞中,Wortmannin剂量为100 nM时可发挥完全抑制作用,增加PtdInsP2水平,且对PtdInsP和 PtdIns没有影响;Wortmannin可动态调控F-actin水平对fMLP刺激的肌动蛋白聚合无影响。[2]在RBL-2H3细胞中,Wortmannin通过结合110-kDa蛋白,不可逆的抑制磷酸肌醇3激酶(PI3-kinase)活性(IC 50为3 nM)对,对Pi4-kinase没有影响。Wortmannin也抑制Fc epsilon RI介导的组胺分泌和白三烯释放,对酪氨酸激酶Lyn无影响。[3]在大鼠脂肪细胞中,剂量为0.1 μM的 Wortmannin可完全抑制胰岛素诱导的己糖吸收,二不影响异丙肾上腺素刺激的脂肪分解活性。[4] 在人脐静脉内皮细胞中,在IGF-1的存在下,Wortmannin抑制胰岛素诱导一氧化氮生产,IC50为500nM。[5]在中国仓鼠卵巢细胞中, Wortmannin以50 μM剂量可抑制DNA双链断裂(DSB)修复,但对DSB水平无影响或单链断裂(SSB)酶活无影响。Wortmannin可加强电离辐射(红外)诱导的细胞毒作用,但本身无毒性。[6] Wortmannin抑制水球样激酶(PLK 1)活性,IC 50为24nM,造成细胞G 2 / M阻滞。[7] 在人巨噬细胞中,Wortmannin增加 Toll样受体(TLR)介导的白细胞介素- 6的积累,EC 50 = 50 nM。在小鼠巨噬细胞中,Wortmannin显着增强TLR诱导一氧化氮合酶(iNOS)的表达和亚硝酸盐积累。Wortmannin激活核因子-κB和上调细胞因子mRNA量。[8] Wortmannin也抑制水球样激酶(PlK)1和PlK 3,在有丝分裂中发挥重要作用。Wortmannin治疗可能导致可减少由DNA损伤诱导的p53丝氨酸20位磷酸化。[9] 在SW 1990细胞中,Wortmannin可抑制透明质酸诱导Akt磷酸化和细胞运动/迁移。[10]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A459 NUDadpB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3K5OlIvPSEQvF2= M3zyRVEuPCCm NXq4[oxwTE2VTx?= M1LwTIVvcGGwY3XzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25idILlZZRu\W62IIfpeIghfGGvb4jp[oVv M3HpZ|I2PDlyM{iz
H1703 MofBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfBfGZ1Oi53IN88US=> NGPnRVUyNTRiZB?= MoqzSG1UVw>? NF7GcVFmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIITy[YF1dWWwdDD3bZRpKHSjbX;4bYZmdg>? NWDVTo54OjV2OUCzPFM>
HUVECs MVzDfZRwfG:6aXPpeJkhSXO|YYm= NIPVN24yODBibl2= M4K3c|I1KGh? MWrheJRmdnWjdHXzJJRp\SCjYoLv[4F1cX[nIHXm[oVkfHNib3[gZ4FtgWOxc3nuJI9vKF[UST3pcoR2[2WmIHP5eI91d3irY3n0fS=> MkPjNlU1PTBzOE[=
APRE-19 MXzBdI9xfG:|aYOgRZN{[Xl? NILsW2Y2KM7:TR?= M1;PWFI1KGh? NUHnXZA6[WKxbHnzbIV{KE[OWj3t[YRq[XSnZDDwdo8ue3W{dnn2ZYww[W62aT3hdI9xfG:|aYOgZYN1cX[rdIm= MV[yOVMzQTZzNx?=
MDA-MB-231 M2rkNWFxd3C2b4Ppd{BCe3OjeR?= MnXLNeKh|ryPwrC= M{XSTlQ5KGh? M3L0WmROW09? NFu0W4tl\WO{ZXHz[ZMhfGinIHPlcIwhe3W{dnn2ZYwhfHKnYYTl[EB4cXSqIEK1JO69VSCxZjDGNUBweiCIMjFCpC=> MVSyOVMxODl|Mh?=
MCF7 MmLiSpVv[3Srb36gRZN{[Xl? MlfZNVAxKG6P M3fwS|I1KGh? NHPWeoxmdGmvaX7heIV{KEV{LXnu[JVk\WRiQWLFMWx2[yCjY4Tpeol1gQ>? NFfUbJozPTF5MkW1Oy=>
HT-29  NWe5V5YyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXWxMlXDqML3TR?= M1zyXlk3KGh? MofQ[IVkemWjc3XzJINmdGxiZ4Lve5RpKHeqaXPoJINidiCkZTDpcohq[mm2ZXSgZpkhU1mQQR?= MlfGNlUxOTJzMkO=
MO59K  NFOxT25EgXSxdH;4bYNqfHliQYPzZZk> NI\JVIw2yqEQvF2= MlvQO{Bl NVXJfIZMTE2VTx?= MWflcohidmOnczD0bIUh[3m2b4TvfIlkcXS7IH;mJIV1d3Cxc3nk[UBweiClaYPwcIF1cW5? M4\N[VI1QTV|NU[x
MO59J NXHObZRxS3m2b4TvfIlkcXS7IFHzd4F6 MV:1xsDPxE1? NXXjVFRjPyCm M1XROGROW09? M2XYeoVvcGGwY3XzJJRp\SCleYTveI95cWOrdImgc4Yh\XSxcH;zbYRmKG:{IHPpd5Bt[XSrbh?= MYmyOFk2OzV4MR?=
MO59K  NEXy[4VCeG:ydH;zbZMhSXO|YYm= NGX5TYEyOCEQvF2= MoHFNlQhcA>? NIjvV|hFVVOR NV25T5NVcW6lcnXhd4V{KHSqZTDEV2IhdGW4ZXygbY5lfWOnZDDifUBmfG:yb4Pp[IUhd3JiY3nzdIxifGmw NFLrTWczPDl3M{W2NS=>
MO59J MX7BdI9xfG:|aYOgRZN{[Xl? NXvVd2V3OTBizszN MXuyOEBp Mn3xSG1UVw>? M2XwNolv[3KnYYPld{B1cGViRGPCJIxmfmWuIHnu[JVk\WRiYomg[ZRweG:|aXTlJI9zKGOrc4DsZZRqdg>? M2Xv[|I1QTV|NU[x
HepG2 MlvqSpVv[3Srb36gRZN{[Xl? NYDUPVl6OTByIH7N M3zRcVAvPSCq MVnEUXNQ MWficI9kc3NiTVGtbY5lfWOnZDDBb5QheGixc4Doc5J6dGG2aX;u NEe3W3YzPDh4M{O1NC=>
A549  MkKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrlPVloOyEEtV5CpC=> MmfaNkBp M3PtZZN2eHC{ZYPz[ZMheGWvZYTy[Zhm\C2rbnT1Z4VlKEGtdDDhcoQhT1ONM98yJIFkfGm4YYTpc44tKFNvcHjhd4Uh[XK{ZYP0MEBk\WyuIHHwc5B1d3OrczDhcoQh[2G|cHHz[U0{KGGldHn2ZZRqd25? NWGz[oxxOjR6NEe4OlM>
A549  M{jVR2Z2dmO2aX;uJGF{e2G7 MXSxNOKh|ryvwrC= NEP6ZXkyPiCq NYDTfY5FTE2VTx?= MoDQcY9lfWyjdHXzJJRp\SCLQW[gdoVxdGmlYYTpc44h[W6mIHPheZNmeyC{ZYTlcpRqd25ib3[gUnAhcW5idHjlJI52[2yndYOu NF3le2UzPDhyMkGxNS=>
SK-N-LO NYfHN5lbTnWwY4Tpc44hSXO|YYm= MmDWNVAxKG6P M1zZNVAvPSCq M2PjTIRm[3KnYYPld{B1cGVic4TpcZVt[W62IHXm[oVkfHNib3[gcY9zeGirbnWgc44hSWu2IIDoc5NxcG:{eXzheIlwdg>? NFrZZ20zPDZ3NE[wOi=>
HL-60 MmS0SpVv[3Srb36gRZN{[Xl? MoC5NE4yyqEQvF2= NFOzXJI4OiCq M1jVNIJtd2OtczDkZZNifGmwaXKtbY5lfWOnZDDj[YxtKGSrZn\ldoVvfGmjdHnvci=> NX7MUI43OjR4MEeyO|M>
HepG2  MX3GeY5kfGmxbjDBd5NigQ>? NHXrPFUzODBibl2= M4nTdlAvPSCq NXfzfW9N[XS2ZX71ZZRmeyCIb4jPJJBpd3OyaH;yfYxifGmxbh?= NFnBNWUzPDV|NUG5Ni=>
H520 NYfqfZdpTnWwY4Tpc44hSXO|YYm= NUHFVXM6OTEEoN88US=> MlPlNUBp NX\4fpRLTE2VTx?= NEXNbGNl\WO{ZXHz[ZMh[2WubIXsZZIheGixc4Doc{1CU1RicILveIVqdiCuZY\lcJM> NEnHTYczPDR2N{mzOS=>
H1975 MnzPSpVv[3Srb36gRZN{[Xl? Mn;kNVDDqM7:TR?= NIjRbFEyKGh? M{O5bmROW09? MmXm[IVkemWjc3XzJINmdGy3bHHyJJBpd3OyaH:tRWtVKHC{b4TlbY4hdGW4ZXzz MWqyOFQ1Pzl|NR?=
MG-63 NXizOYZSSXCxcITvd4l{KEG|c3H5 NEnGd2syOCEEtV2= MXexNkBp MX3lcohidmOnczDEVE1qdmS3Y3XkJIFxd3C2b4Ppdy=> NILCfYwzPDN3OEOwNS=>
5637 NWHY[JptSXCxcITvd4l{KEG|c3H5 MkHQNVDDqM7:TR?= MkntOFAhdWmw MlTrdoV3\XK|ZYOgdFIyX0GIMTDlfJBz\XO|aX;uMEBETEtiZYjwdoV{e2mxbjygZY5lKGOnbHygbY5pcWKrdHnvckBqdmS3Y3XkJIJ6KG[3Y3;p[IFv NFXLUWczPDN|M{i2PC=>
HEK-293 NVKwVY5ITnWwY4Tpc44hSXO|YYm= NEfKXlUyPTCwTR?= M3f3RlE3KGh? M{LGW2ROW09? NXGxenRL\GWlcnXhd4V{KEOUVDDhZ5Rqfmm2eR?= Mmi5NlQ{OjR|Nk[=
SW480  M4n3O2Z2dmO2aX;uJGF{e2G7 M{WzflE2OG6P NXPR[IdoOjBiaB?= NUG0bHZYTE2VTx?= MUHy[YR2[2W|IHPlcIx2dGG{IHHjZ5VufWyjdHnvckBw\iEQsj3jZZRmdmmw Ml;CNlQ{OjR|Nk[=
HepG2 NX;3So9MTnWwY4Tpc44hSXO|YYm= M4XFXVExOCCwTR?= M3nqbFI1KGh? M3fL[oF1fGWwdXH0[ZMhfGinIHPvcI9vcWW|IH;mJJRp\SC2dX3vdkBk\WyuczD3bZRpKHWycnXneYxifGmxbjDv[kBCc3Rz NFPa[nczPDJ7N{WxNC=>
HCT 116  NWewblNRTnWwY4Tpc44hSXO|YYm= NWrUZnR[OTByIH7N NEfkW4ozPCCq NXrFS|F{[XS2ZX71ZZRmeyC2aHWgZ49td26rZYOgc4YhfGinIIT1cY9zKGOnbHzzJJdqfGhidYDy[Yd2dGG2aX;uJI9nKEGtdEG= MnX2NlQzQTd3MUC=
BEL/FU NYjPeFJoTnWwY4Tpc44hSXO|YYm= MU[xJI1O NVzFd|BvOjRiaB?= M2nEWIRm[3KnYYPld{Bxem:2ZXnuJIxmfmWuczDv[kB1cGViUFmzT{9Cc3RicHH0bJdigQ>? NWnNV4l2OjR{M{KwPVk>
Huh7  NInRSnFHfW6ldHnvckBCe3OjeR?= MYSzxsDPxE1? NW\UcYdrOSCq MlLrdoVlfWOnczD0bIUhfmm{dYOg[Y51enliaX70c{B1cGViY3XscJM> NHvVbG4zPDF6NEG5Oi=>
A-375 MVvBdI9xfG:|aYOgRZN{[Xl? MmPYOE85KM7:TR?= MV6yOEBp NGn6PINmdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg Mn3ENlQyOTNzN{O=
A-375-TS  NIS4W4lCeG:ydH;zbZMhSXO|YYm= NY\2d3ZQPC96IN88US=> MWCyOEBp NGj6TZJmdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg NFzqdJQzPDFzM{G3Ny=>
Mel-HO M3SwdWFxd3C2b4Ppd{BCe3OjeR?= MWK0M|gh|ryP NWOzc2xzOjRiaB?= MmWw[Y5p[W6lZYOgWHJCUUxvaX7keYNm\CCjcH;weI9{cXQEoB?= Mo\uNlQyOTNzN{O=
Mel-HO-TS MlrGRZBweHSxc3nzJGF{e2G7 MkjSOE85KM7:TR?= NWfwe3M2OjRiaB?= MXPlcohidmOnczDUVmFKVC2rbnT1Z4VlKGGyb4D0c5Nqe8Li NX:2R413OjRzMUOxO|M>
MeWo MnXrRZBweHSxc3nzJGF{e2G7 NUDmb5B6PC96IN88US=> Ml34NlQhcA>? M2W2NYVvcGGwY3XzJHRTSUmOLXnu[JVk\WRiYYDvdJRwe2m|wrC= NI\neJozPDFzM{G3Ny=>
Mel-2a MXPBdI9xfG:|aYOgRZN{[Xl? NV;4PGFnPC96IN88US=> M2Dx[lI1KGh? NE\nS5VmdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg M3\DUFI1OTF|MUez
MDA-MB-231 MlT6SpVv[3Srb36gRZN{[Xl? NVPHTpFkOOLCk{SwNEBvVQ>? M4O1V|QhcA>? MYnzeZBxemW|c3XzJGFsfCCyaH;zdIhwenmuYYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NXS2dpg5OjJ7ME[yOVk>
MDA-MB-231 M1;LNGZ2dmO2aX;uJGF{e2G7 MU[0NFAhdk1? NXHKTml[PCCq MlLB[IVkemWjc3XzJG1OWC17IHHu[EBKVC16IIDyc5RmcW5iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MlnrNlI6ODZ{NUm=
Jurkat MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX[wMlI2NTFwMkWg{txO MVmyOE81QCCq M{G3VGROW09? M3X6VIlvcGmkaYTzJINmdGxicILvcIln\XKjdHnvckBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLTDk[ZBmdmSnboSgcYFvdmW{ M3i2U|E6PzV5MUi1
Namalwa M4T6W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVGwMlI2NTFwMkWg{txO NYT3S|FlOjRxNEigbC=> NIPQT|lFVVOR MoPkbY5pcWKrdIOgZ4VtdCCycn;sbYZmemG2aX;uJIlvKGKxdHigeIlu\S1iYX7kJIRwe2VvIHTldIVv\GWwdDDtZY5v\XJ? M{LSc|E6PzV5MUi1
Jurkat Mn\uRZBweHSxc3nzJGF{e2G7 M4q2[FAvOjVvMT6yOUDPxE1? MWiyOE81QCCq MWjEUXNQ NXPOUVNscW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBjd3SqIITpcYUuKGGwZDDkc5NmNSCmZYDlcoRmdnRibXHucoVz MontNVk4PTdzOEW=
Namalwa MlzqRZBweHSxc3nzJGF{e2G7 NGTOb|cxNjJ3LUGuNlUh|ryP MV:yOE81QCCq MlnvSG1UVw>? MnHzbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMUBl\XCnbnTlcpQhdWGwbnXy MWmxPVc2PzF6NR?=
K562 NGXxVIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHXfWloOjRiaB?= Ml74TWM2OD1{NdMxNE4yPCCwTR?= M3nVTlE6PjZ{M{[x
SW1990 M3rtSmZ2dmO2aX;uJGF{e2G7 M1nrU|AvODFvMTFOwG0> MnuyNUBp NEL2fmJqdmirYnn0d{BJSS2rbnT1Z4VlKEGtdDDwbI9{eGixconsZZRqd25? M3uzNlE6PDZ7MEKw
RT112  MkXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVyxNOKh|ryP MmLKNlQhcA>? M4PYdWROW09? MUnk[YNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gS|IwVSClZXzsdy=> NFrafFgyQDd6N{izNi=>
MHG-U1 NHT5c3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLZNVDDqM7:TR?= MXuyOEBp NYC5NVNjTE2VTx?= M3zESYRm[3KnYYPld{B1cGVicILvdI9zfGmxbjDv[kBIOi:PIHPlcIx{ NYjq[3Y2OTh5OEe4N|I>
SMMC-7721 NHzFdHVCeG:ydH;zbZMhSXO|YYm= NXXsVoNkOjBywrDuUS=> NYDs[mgyOjRiaB?= M3rscIlv[3KnYYPld{BEUFhvaX7keYNm\CCjcH;weI9{cXN? Mmq1NVc2PTdzOUG=
SMMC-7721 NGn2ZXhHfW6ldHnvckBCe3OjeR?= M3fkZ|IxOMLibl2= MUiyOEBp NEnRbVN2eC2{ZXf1cIF1\XNizsKxMFRIXDFiZYjwdoV{e2mxbh?= MkHLNVc2PTdzOUG=
HeLa MXTGeY5kfGmxbjDBd5NigQ>? NUPCfmFHOTBywrDuUeKh NYPWeVcxOSCq NFXwbJlidHSncoOgeIhmKG2xcoDoc4xw\3lib3[geIhmKHS{YX7z[oVzemmwIILlZ5lkdGmwZzDjc41x[XK2bXXueC=> M2XUU|E3QDlyOUG1
MRC5VI NUGxU3hQTnWwY4Tpc44hSXO|YYm= NX;DT5VMOTJwNTDtUS=> NYfseWV[OC53IHi= NYrFelRzTE2VTx?= NYj3VJRq[WKxbHnzbIV{KHSqZTDT[ZI1PzNxVHjyN|A5yqCyaH;zdIhwenmuYYTpc44hd2ZiQXv0VGtDyqB? MmW0NVYzOjd|OUS=
AT5BIVA MmrzSpVv[3Srb36gRZN{[Xl? NWqyXZQ2OTJwNTDtUS=> NUPSe|RvOC53IHi= Mn;5SG1UVw>? NFToN5Ri[m:uaYPo[ZMhfGinIGPldlQ4Oy:WaIKzNFjDqHCqb4PwbI9zgWyjdHnvckBw\iCDa4TQT2LDqA>? NXv5TnQ{OTZ{MkezPVQ>
M059J MlewSpVv[3Srb36gRZN{[Xl? NX7aflVTOTJwNTDtUS=> NGn1T5cxNjViaB?= NXTW[4oxTE2VTx?= M{LBeIFjd2yrc3jld{B1cGViU3XyOFc{N1SqckOwPOKheGixc4Doc5J6dGG2aX;uJI9nKEGtdGDLRuKh NIfPUlkyPjJ{N{O5OC=>
HeLa MYDGeY5kfGmxbjDBd5NigQ>? NGLXdmUyOi53IH3N NGfrS2kxNjViaB?= NXjPdIg1TE2VTx?= MWPhZo9tcXOqZYOgeIhmKFOnckS3N{9VcHJ|MElCpJBpd3OyaH;yfYxifGmxbjDv[kBCc3SSS1NCpC=> MUWxOlIzPzN7NB?=
N2a MlPLRZBweHSxc3nzJGF{e2G7 NYX1bGl3OC5zLUGwJO69VQ>? NIHaWpgzKGh? MVTpcoR2[2W|IHTlZ5Jm[XOnZDDj[YxtKH[rYXLpcIl1gSCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>? NFvBWmkyPTh2Mke2Oy=>
Jurkat  MVjLbY5ie2ViQYPzZZk> MXjJR|UxKG:oIEK0JI5O NYDsUGlqOTV4NkS1NVk>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT / p-GSK3β / GSK3β / Bcl-xl / Bax / Caspase-3 / Cleaved caspase-3; 

PubMed: 25344912     


Four esophageal cancer cell lines were treated with different concentrations of wortmannin for 48 h, and cell lysates were collected for Western blot analysis of p-AKT, AKT, p-GSK3β, GSK3β, Bcl-xL, Bax, caspase-3, and cleaved caspase-3.

25344912
Immunofluorescence
DNMT1; 

PubMed: 24001151     


PC3 cells were treated with DMSO or wortmannin (1 μM) for 24 hours. DNMT1 and DNMT3B cellular localization was visualized by immunofluorescent staining. After 24h of treatment, cells were fixed in methanol, incubated with the indicated antibodies, stained with Alexa Fluor 594-tagged secondary antibodies and counterstained with DAPI. Slides were then mounted and examined under a fluorescence microscope. The bright field images of PC3 cells treated with DMSO or wortmannin (1 μM) for 24 hours are shown (right panel).

24001151
Growth inhibition assay
Cell viability; 

PubMed: 25344912     


MTT assay was used to determine the effects of different concentrations of wortmannin on viability of esophageal cancer cells lines KYSE150, HKESC-1, KYSE270, and T.Tn. Bars, SD; * P < 0.05; **, P < 0.01; ***, P < 0.001 compared with DMSO-treated cells.

25344912
体内研究 Wortmannin剂量为1mg/kg可抑移植瘤小鼠中SW1990的腹膜转移,无重量损失。[10] Wortmannin抑制在小鼠正常组织(肺,心脏和脑组织匀浆)及肿瘤组织中的phosphatidylinositide 3- B激酶(PKB)/磷酸化Akt,在剂量为0.7 mg/kg时无死亡或急性毒性。与gemcitabine联合使用时,可大大增加细胞凋亡和抑制原位肿瘤生长,两种药物单独使用都无以上效果。[11]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[11]
+ 展开
  • Animal Models: 人胰腺癌细胞 PK1s.c.和原位注入SCID免疫缺陷小鼠。
  • Formulation: 0.4 mg/mL 溶于 DMSO, 使用时用 0.9% NaCl 稀释
  • Dosages: 0.175, 0.35, 和0.7 mg/kg
  • Administration: 静脉注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 85 mg/mL (198.39 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 428.43
化学式

C23H24O8

CAS号 19545-26-7
储存条件 powder
in solvent
别名 KY 12420

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

如果有其他问题,请给我们留言。

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID