LY294002

目录号:S1105 别名: SF 1101, NSC 697286

LY294002 Chemical Structure

Molecular Weight(MW): 307.34

LY294002 是首个合成的已知抑制PI3Kα/δ/β的小分子,在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM;在溶液中比在Wortmannin中稳定,也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶,还能够结合一些与PI3K家族无关的新型靶点。

规格 价格 库存 购买数量  
RMB 1570.11 现货
RMB 1188.16 现货
RMB 2196.01 现货
RMB 6298.74 现货
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客户购买Selleck的此次产品后发表的文献73篇:

客户使用该产品的15个实验数据:

  • Type I IFNs induce ISG expression in a serine phosphorylation-independent manner. Cells were treated with kinase inhibitors (SB203580, rottlerin, Ly294002, PD98059, and SP600125) before stimulation with IFN-a and IFN-b for 6 hours. Cell lysates were used in western blotting and probed for serine phosphorylation.The relative amounts of pSTAT1 S727 in (up-graph) were quantied and normalized to an untreated control (below-graph).

    Hepatology 2014 59(4), 1262-72. LY294002 purchased from Selleck.

    different signal-inhibitory profile by applying 120 nmol/L BGT226, 20 μmol/L LY294002, or 100 nmol/L rapamycin in FaDu cells.

    Clin Cancer Res 2011 17, 7116-7126. LY294002 purchased from Selleck.

  • Stimulation response of p37d-expressing cells. (c) Western blot of 5 min serum stimulated HEK-293 cells stably expressing p37d, p110d or control, with the presence of inhibitors as indicated at the top.

    Oncogene 2012 31, 3277–3286. LY294002 purchased from Selleck.

    Immunofluorescence and flow cytometric analyses of the translocation of ABCG2 in MCF-7 FLV1000 cells before and after treatment with the PI3K inhibitor LY294002. Confocal microscopy of MCF-7 FLV1000 cells after a 16-h treatment with 2 uM or 4 uM of LY294002 was performed as described in Figure.

    Br J Pharmacol 2013 70(5), 1137-51. LY294002 purchased from Selleck.

  • SCC61 or Detroit 562 xenografted tumors respond to treatment by PI3K inhibitor, LY294002 alone, or in combination with gefitinib. % tumor growth is shown. By two-way ANOVA, the effect of treatment and time is considered significant;

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

    Inhibition of EGFR and PI3K/AKT pathway has additive effects in SCCHN cell lines with constitutively active PIK3CA mutations, while removing negative regulation of PI3K/AKT pathway increases resistance in sensitive SCCHN cell line. A, results from MTT assays on cells treated with PI3K inhibitor, LY294002, and gefitinib.

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

  • Lyn-PI3K-BTK signaling is required for the inhibitory effect of anti-CD180 on the activation of IFN-α signaling. (a and b) Isolated murine splenic B220+ B cells were pretreated with dasatinib (5 μM), a Lyn inhibitor; LY294002 (5 μM), a PI3K inhibitor; ibturinib (1 μM), a BTK inhibitor; enzastaurin (1 μM), a PKC β inhibitor; U0126 (3 μM), an ERK inhibitor; SP600126 (2 μM), a JNK inhibitor or SB20358 (1 μM), a p38 inhibitor for 1 h, followed by stimulation with anti-CD180 antibody (0.2 μg/mL) or mouse IFN-α (1000 U/mL) for 4 h. qPCR analysis of the expression of IFIT1 (a) and MX1 (b). (c) Isolated murine splenic B220+ B cells were pretreated with dasatinib (5 μM), LY294002 (5 μM), and ibturinib (1 μM) for 1 h, followed by stimulation with anti-CD180 antibody (0.2 μg mL−1) or mouse IFN-α (1000 U mL−1) for 40 min. Western blot analysis of the phosphorylation level of STAT-2. The data shown represent the mean values of three independent experiments, and the error bars represent the SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by ANOVA; ns denotes p > 0.05.

    Cell Mol Immunol, 2017, 14(2):192-202. LY294002 purchased from Selleck.

    The effects of GLP-1R siRNA and LY294002 on neurological score at 24 hours after subarachnoid hemorrhage (SAH) in Ex-4 treated rats.

    Neuropharmacology, 2018, 128:142-151. LY294002 purchased from Selleck.

  • Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

    Comparative effects of wortmannin and LY294002 on ADRP and β -casein synthesis. Con fluent HC11 cells were treated according to the standard procedure in the absence or presence of 4 ng/ml EGF either with or without 100 nM wortmannin or 50μM LY294002. Cellular proteins were analyzed by SDS-PAGE and immunoblot successively probed with anti-ADRP, anti-β-casein, anti-β -actin antibodies and their respective HRP-conjugated secondary antibodies. ECL signals were quantified by densitometry and normalized to β-actin. Each experimental condition was performed in triplicate. Percentages of inhibition for both inhibitor and hormonal treatments are indicated in the text.

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

  • Inhibition of PI3K, ERK and mTOR prevents the activation of S6K1 and S6 induced by suppression of PKD1 activity. A549 cells were incubated in the absence (-) or presence of either 5 uM Kb or 5 uM Kb and 20 uM LY294002 or 5 uM Kb and 10 uM BKM120 (as indicated) for 1 h prior to stimulation of cells with 50 nM PMA for 30 min and 1 h.

    Int J Biochem Cell Biol 2015 60, 34-42. LY294002 purchased from Selleck.

    IGF1 increases apical fibronectin in late stage blastocysts. Representative confocal images of blastocysts stained for fibronectin (Green) and DAPI (Blue). Day 5 blastocysts were cultured in SS medium for 24 (A and B), 48 (C and D) or 72 h (E-H) in the absence (A, C and E) or presence (B, D and F) of 10 ng/ml IGF1 or in the presence of a PI3 Kinase inhibitor (5 uM LY294002 + 10 ng/ml IGF1) (G) or in the presence of 10 ng/ml IGF1, attached to a coverslip (H). Scale bars represent 20 mm. Red line indicates location of coverslip. Arrow indicates location of the inner cell mass (ICM). The following total number of blastocysts were analysed, from at least 3 experimental repeats in each treatment; 24 in the SS group (24 h), 21 in SS plus IGF1 (24 h), 26 in SS (48 h), 30 in SS plus IGF1 (48 h), 40 in SS (72 h), 59 in SS plus IGF1 (72 h), 13 in SS plus IGF1 + LY294002 (48 h) and 15 blastocysts were analysed that were attached to a coverslip (72 h).

    Hum Reprod 2015 30(2), 284-98. LY294002 purchased from Selleck.

  • PTEN transfection or inhibition of the PI3K-AKT and IGF-1R signaling pathways increases the trastuzumab sensitivity of NCI-N87/TR cells. After treating the cells with a PI3K inhibitor (LY294002), the expression of AKT and P-AKT proteins was assessed by Western blotting. Tubulin expression indicated equal loading. All gels run under the same experimental conditions and the experiments were repeated 3 times. The representative images were cropped and shown. Control: cells without any treatment.

    Sci Rep 2015 5, 11634. LY294002 purchased from Selleck.

    We treated all of drugs in T47D which has a PI3KCA H1044R mutation with the concentration shown below for 1 hour and performed western blot analysis using antibodies to phospho-AKT(SERINE 472), and total AKT.

     

     

    2010 Saraswati Sukumar of Johns Hopkins University School of Medicine. LY294002 purchased from Selleck.

  • T47Dcells were pretreated with 100 ng/ml EGF for 20 min and then treated with the indicated concentrations of LY294002 for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. LY294002 purchased from Selleck.

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 LY294002 是首个合成的已知抑制PI3Kα/δ/β的小分子,在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM;在溶液中比在Wortmannin中稳定,也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶,还能够结合一些与PI3K家族无关的新型靶点。
特性 LY294002是选择性的, 可渗透细胞的特定PI3K抑制剂,可作用于酶的ATP结合位点,且抑制自体吞噬的螯合作用。
靶点
p110α [1]
(Cell-free assay)
p110δ [1]
(Cell-free assay)
p110β [1]
(Cell-free assay)
DNA-PK [6]
(Cell-free)
DNA-PK [6]
(Cell-free assay)
0.5 μM 0.57 μM 0.97 μM 1.4 μM 1.4 μM
体外研究

LY294002是一种非特异性抑制剂,除了class I PI3Ks,它同样还能抑制其他类型PI3Ks、mTOR、DNA-PK以及其他蛋白激酶,如CK2和Pim-1[5]。LY294002使Akt/PKB失活,因此抑制细胞增殖和诱导细胞凋亡。 LY294002作用于结肠癌细胞系,使磷酸化Akt (Ser473)的表达下降, 明显抑制生长和诱导凋亡。[2] LY294002作用于肿瘤细胞,诱导明显的核固缩和减少细胞质体积。因此,在体外,LY294002明显抑制卵巢癌细胞增殖。LY294002诱导细胞停在G1期,导致几乎全部的黑色素瘤细胞增殖被抑制,部分MG-63 (骨肉瘤细胞系)增殖被抑制。LY294002作用于细胞周期的效果可用于研究PI3K激活通路和癌细胞周期之间关系。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-231 cells MWXD[YxtKGmwdnHzbY9vKGG|c3H5 NYPjcIZqOjRiaB?= NFTmNWdKdmirYnn0bY9vKG:oIHXwbZRp\WyrYXyg[5Jwf3SqIH\hZ5Rwei2rbnT1Z4VlKGOnbHygcYloemG2aX;uJI9nKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgdJJmNWmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JINmdGxiaX72ZZNqd25iYYPzZZktKEmFNUC9NE4{QCEQvF2u NGHzNlQzOjhyNEGwPC=>
Sf21 cells MXXGeY5kfGmxbjDhd5NigQ>? M4\UU2lvcGmkaYTpc44hd2ZibX;1d4Uhf2muZDD0fZBmKFCLM1vhcJBp[SCneIDy[ZN{\WRiaX6gV4YzOSClZXzsd{Bkdy2neIDy[ZN{cW6pIF6teIVzdWmwYXygTIl{NXSjZ3fl[EBpfW2jbjDwPFViKHW|aX7nJGwu[WyyaHGtdIhwe3CqYYTp[JltcW6xc3n0c4whe3Wkc4TyZZRmKGK7IGTMR{Bj[XOnZDDwbI9{eGixcjDpcYFocW6pLDDJR|UxRTBwNTFOwG0> MlnSNlI2OjB4M{C=
Sf21 cells M2TPSWZ2dmO2aX;uJIF{e2G7 MVXJcohq[mm2aX;uJI9nKG2xdYPlJJJm[2:vYnnuZY51KFCLM1vhcJBp[SCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMucW6oZXP0[YQhW2Z{MTDj[YxteyxiSVO1NF0xNjVizszNMi=> MVKxPVc1QDJ4OR?=
Sf9 cells MkPDSox2d3Knc3PlcpQheG:uYYLpfoF1cW:wIHHzd4F6 NWe2T5pOUW6qaXLpeIlwdiCxZjDoeY1idiCSSUPLZYxxcGFiZYjwdoV{e2WmIHnuJHNnQSClZXzsd{BjgSCobIXvdoV{[2WwdDDwc4xiemm8YYTpc44h[XO|YYmsJGlEPTB;MD61OUDPxE1w M3\tPVIyOTJzNkOx
THP1 cells M4LhXGZ2dmO2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKFOFRkGtbY5lfWOnZDDQT2IwSWu2IIDoc5NxcG:{eXzheIlwdiCrbjDUTHAyKGOnbHzzMEBKSzVyPUGuNFEh|ryPLh?= MlnQNVY4QDl5NEK=
human PC3 cells M{nJbWZ2dmO2aX;uJIF{e2G7 NGf6XJA{OCCvaX6= MmrMTY5pcWKrdHnvckBw\iCSSUPLJIlvKGi3bXHuJHBEOyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDBT3QheGixc4Doc5J6dGG2aX;uJIF1KHOncnnu[UA1PzNiYX\0[ZIhOzBibXnud{BjgSCHTFnTRUwhUUN3ME2xMlPPxE1w MnnLNlM1OTByMEW=
HeLa (human carcinoma) cells. NWHPU2pWTnWwY4Tpc44h[XO|YYm= MWjJckB3cXS{bzDpcohq[mm2aX;uJI9nKESQQT3k[ZBmdmSnboSgdJJwfGWrbjDrbY5ie2VqRF7BMXBMMSCocn;tJGhmVGFiKHj1cYFvKGOjcnPpco9u[SliY3XscJMtKEmFNUC9NU41KM7:TT6= NWXNb5A3OTV4NUi4O|A>
THP1 cells NVXoV3RQTnWwY4Tpc44h[XO|YYm= M{C1cGlvcGmkaYTpc44hd2ZiTVPQNU1qdmS3Y3XkJHBMSi:Da4SgdIhwe3Cqb4L5cIF1cW:wIHnuJHRJWDFiY3XscJMtKEmFNUC9NU43PSEQvF2u NELlcFcyPjd6OUe0Ni=>
HeLa cells NVXWPJlHTnWwY4Tpc44h[XO|YYm= M3nCSWlvcGmkaYTpc44hd2ZibWTPVkBxem:2ZXnuJIl{d2yjdHXkJIZzd21iSHXMZUBk\WyuczygTWM2OD1{LkWg{txONg>? MoPHNVU3PTh6N{C=
human MCF7 cells NG\KSFlEgXSxdH;4bYPDqGG|c3H5 NHi3U|BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGmwIIDy[ZNmdmOnIH;mJFIxKM7:TTDjbIxwem:zdXnu[UBjgSCVUlKgZZN{[XluIFfJOVA:Oi54MzFOwG0v MoTONVg3QTF6OUS=
HeLa cells NYS4TFJHTnWwY4Tpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKEG[LUe1NFMh[mmwZHnu[{B1dyC{ZXPvcYJqdmGwdDDQcIs{KGW6cILld5Nm\CCrbjDI[WxiKGOnbHzzJIJ6KFenc4Tldo4h[myxdDygTWM2RTNizszNMi=> M3XuV|E4OTN3MkS4
human MCF7 cells NVrFOYRWS3m2b4TvfIlkyqCjc4PhfS=> NIfL[YdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGmwIIDy[ZNmdmOnIH;mJFExKHWPIHPocI9zd3G3aX7lJIJ6KFOUQjDhd5NigSxiR1m1NF0{NjB6IN88UU4> NFzRTI8yQDZ7MUi5OC=>
human MCF7 cells NX\qdZJ5S3m2b4TvfIlkyqCjc4PhfS=> NELUPJpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGK7IGPSRkBie3OjeTygS2k:Oy5zNjFOwG0v M1LCWFE5PjlzOEm0
human MDA-MB-231 cells NGnnd3ZEgXSxdH;4bYPDqGG|c3H5 MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHnuJJBz\XOnbnPlJI9nKDJyIN88UUBkcGyxcn;xeYlv\SCkeTDTVmIh[XO|YYmsJGdKRTNwM{Kg{txONg>? M1XmUVE5PjlzOEm0
human MDA-MB-231 cells NUjpPJI4S3m2b4TvfIlkyqCjc4PhfS=> NH[5NGNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIlvKHC{ZYPlcoNmKG:oIEGwJJVOKGOqbH;yc5F2cW6nIHL5JHNTSiCjc4PhfUwhT0l3ME20MlM2KM7:TT6= M4HNU|E5PjlzOEm0
human MDA-MB-468 cells M4HMU2N6fG:2b4jpZ:Kh[XO|YYm= NWDFWXJDS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BqdiCycnXz[Y5k\SCxZjCyNEB2VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZnwxIxiSVO1NF01Njd4IN88UU4> M2\lPFE5PjlzOEm0
human HCT116 cells MmrYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV20PEBp MofhS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJHBKO0ujbIDoZUBJOTB2N2KgcZV1[W62IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS5|IN88US=> NXfqZ3hDOjJ{MUK3NlE>
human HCT116 cells M4nORWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mke2OFghcA>? MVTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21Mlgh|ryPLh?= MYSyNlIyOjd{MR?=
HeLa cells NUL6c255SmmwZHnu[{Bi\m[rbnn0fUBie3OjeR?= MVzCbY5lcW6pIHHm[olvcXS7IH\vdkBRUTNva3nuZZNmKGm|b3zheIVlKG[{b32gTIVN[SClZXzsd{whU2l;NjFOwG0> MWexOVY2QDh5MB?=
human HCT116 cells MnPOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1P3TFQ5KGh? MlGwS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJHBKO0ujbIDoZUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPU[uO{DPxE1w M2jjXVIzOjF{N{Kx
MDA-MB-231 cells M3PxeWN6fG:2b4jpZ:Kh[XO|YYm= MnjvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9Ok44OSEQvF2u MWCxPFY6OTh7NB?=
Sf9 cells MV3GeY5kfGmxbjDhd5NigQ>? NIfNe2RKdmirYnn0bY9vKG:oIFPLNkBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|LDDJR|UxRTZwOTFOwG0v NFH4OWUyQDN{MUexOi=>
human U87MG cells NF3XUZJHfW6ldHnvckBie3OjeR?= M1jYbWlvcGmkaYTpc44hd2ZiR2PLN{1j\XSjIHnuJIh2dWGwIGW4O21IKGOnbHzzJIJ6KEWOSWPBMEBKSzVyPUiuNUDPxE1w M2\Id|E5OzR3NkC5
human MDA-MB-468 cells MVTDfZRwfG:6aXRCpIF{e2G7 MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHnuJJBz\XOnbnPlJI9nKDFyIIXNJINpdG:{b4H1bY5mKGK7IGPSRkBie3OjeTygS2k2OD16LkKg{txONg>? NYjzT21EOTh4OUG4PVQ>
human A375 cells MVrQdo9tcW[ncnH0bY9vKGG|c3H5 NYPjdZg4PDZiaB?= NYLHPFQ1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IES2JIhzeyxiSVO1NF05NjRizszNMi=> MkHCNVcxPDl{NEi=
human A375 cells M3jqNXBzd2yrZnXyZZRqd25iYYPzZZk> M{\OZmlvcGmkaYTpc44hd2Zic3XyeY0ucW6mdXPl[EBxem:uaX\ldoF1cW:wIH;mJIh2dWGwIFGzO|Uh[2WubIOsJGlEPTB;OD60JO69VS5? NU\WU|J7OTd4MEG3N|k>
human HL60 cells MW\DfZRwfG:6aXRCpIF{e2G7 M2HWdlczKGh? NVW5XG5uS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVkvQTRizszNMi=> NVnXSWFIOjJ2OEC4OVE>
mouse Raw264 macrophage NWTacVlRTnWwY4Tpc44h[XO|YYm= NHLIe25KdmirYnn0bY9vKG:oIFO1ZU1u\WSrYYTl[EBRU0JxQXv0JJBpd3OyaH;yfYxifGmxbjDpckBud3W|ZTDSZZczPjRibXHjdo9xcGGpZTygTWM2OD1zMDFOwG0v NUP4dHc1OTZ5OEm3OFI>
Sf9 cells Ml;wSpVv[3Srb36gZZN{[Xl? NInKcZhKdmirYnn0bY9vKG:oIFjpd{11[WepZXSgZo93cW6nIGDJN2sh\XiycnXzd4VlKGmwIGPmPUBk\WyuczygTWM2OD1zMDFOwG0v M{XwR|ExQTl6M{Wx
human MDA-MB-468 cells MX7DfZRwfG:6aXRCpIF{e2G7 MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHL5JHNTSiCjc4PhfUwhUUN3ME2xNE41KM7:TT6= M3fsRlE5PjlzOEm0
human PC3 cell MontVJJwdGmoZYLheIlwdiCjc4PhfS=> M1i3eFczKGh? MYXJcohq[mm2aX;uJI9nKGi3bXHuJHBEOyClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhPzJiaILzJIJ6KHOyZXP0do9xcG:2b33leJJq[yCjbnHsfZNqeyxiSVO1NF0yOi5zIN88UU4> M4PXSVI{PDFyMEC1
human HL60 cells MoLOVJJwdGmoZYLheIlwdiCjc4PhfS=> NETwfVM4OiCq M4rYU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHid49z[mGwY3WgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zOD60N{DPxE1w NVzXWGhlOjV4OUO3PFc>
human BT474 cell M2fENnBzd2yrZnXyZZRqd25iYYPzZZk> MmLJO|IhcA>? MlX1TY5pcWKrdHnvckBw\iCqdX3hckBDXDR5NDDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IIPw[YN1em:yaH;0c41mfHKrYzDhcoFtgXOrc,-8kEBKSzVyPUKwMlch|ryPLh?= NXLKUIhTOjN2MUCwNFU>
THP1 cells M1\nOGZ2dmO2aX;uJIF{e2G7 NGrFUWlKdmirYnn0bY9vKG:oIF3DVFEucW6mdXPl[EBkcGWvb4ThfIl{KG:oIGTIVFEh[2WubIOsJGlEPTB;M{eg{txONg>? NH;rS5kyPjd6OUe0Ni=>
human 184B5 cells MmTnR5l1d3SxeHnjxsBie3OjeR?= NULZS5BwS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hOTh2QkWgZ4VtdHNiYomgV3JDKGG|c3H5MEBIUTVyPUO5MlM4KM7:TT6= NEP3dGUyQDZ7MUi5OC=>
human KB cells MV;DfZRwfG:6aXRCpIF{e2G7 M{\HdVczKGh? Mn\KR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD12ND63OkDPxE1w MVeyNVk1PTJ3MB?=
mouse RAW264.7 cells NELzNWdHfW6ldHnvckBie3OjeR?= M2\0[VI2KM7:TR?= NIjIPWYzOCCq MYfJcohq[mm2aX;uJI9nKFCLM1uvRWtVKGmwIH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhcU6RUzDwdo91\WmwIHX4dJJme3Orb36gZZQhOjVizszNJIFnfGW{IEKwJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M{TxWVI1Ojl7NkG2
human A2780 cells NGnuNJdCeG:ydH;zbZMh[XO|YYm= NYfiXIFWOiEQvF2= NYPZS3U{OTJiaB?= NXK1dY1[UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBkcXOybHH0bY4uemW|aYP0ZY51KGi3bXHuJGEzPzhyIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJJBpd3OyaH;yfYxifGWmIFHreEBt\X[nbDDheEAzKHWPIHHmeIVzKDF{IHjyd{BjgSCrbX31co9jdG:2IHHuZYx6e2m|Lh?= M{WwW|E4Pjh2MEG4
human SW480 cells NH7wb3dHfW6ldHnvckBie3OjeR?= M1K0blIxKM7:TR?= NVfUboh{OjRiaB?= M2fheGRm[3KnYYPlJIlvKHO3co\peolvKGW6cILld5Nqd25iaX6gbJVu[W5iU2e0PFAh[2WubIOgZZQhOjBidV2gZYZ1\XJiMkSgbJJ{KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= NIq5THAyPzR5Mkm2Ni=>
mouse mast cells NVn3PGJJTnWwY4Tpc44h[XO|YYm= NXfzOGxXOjBizszN NFz6O4FKdmirYnn0bY9vKG:oIGPDSk1qdmS3Y3XkJHBMSi:Da4SgdIhwe3Cqb4L5cIF1cW:wIHnuJI1wfXOnIH3hd5Qh[2WubIOgZZQhOjBizszN NX;DUmVlOTZ5OEm3OFI>
human BJ cells NXTEOZZWTnWwY4Tpc44h[XO|YYm= MV:yOUDPxE1? NWTCdXk1OiCq NVX4bHFnUW6qaXLpeIlwdiCxZjDQTVNMKHOrZ37hcIlv\yCrbjDQSGdHNXO2aX31cIF1\WRic3XyeY0he3Sjco\l[EBpfW2jbjDCTkBk\WyuczD0doFve2[nY4Tl[EB4cXSqIIDsZZNucWRiRVfGVE9RUCCmb33hbY4hd2ZiQXv0JIF{e2W|c3XkJIF{KHO3cIDy[ZN{cW:wIH;mJHBJNUWJRmCgdoVlcXO2cnnieZRqd25idH:gdIxie22jIH3lcYJz[W6nIHH0JFI2KHWPIITy[YF1\WRiMjDodpMh[mWob4LlJHBFT0ZiY3jhcIxmdmenIHL5JINwdm[xY3HsJI1q[3Kxc3PvdJk> MoLqNVY4PjdyOEW=
human IGROV1 cells NFzFXVFHfW6ldHnvckBie3OjeR?= NHW4O|EzPC92ODDo M1W0WmlvcGmkaYTpc44hd2ZiUFmzT{9Cc3RiaX6gbJVu[W5iSVfSU3YyKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIIDoc5NxcG:{eXzheIVlKEGNVDDs[ZZmdCCjdDCxJJRwKDVidHnt[ZMhUUN3MDDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NEDSWIIzOTJzNkG1NS=>
human IGROV1 cells MXjGeY5kfGmxbjDhd5NigQ>? NVq3ZWlsOjRxNEigbC=> MlLHTY5pcWKrdHnvckBw\iCSSUPLM2FsfCCrbjDoeY1idiCLR2LPWlEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY4njcIlvKERzIHzleoVtKGG2IEGgeI8hPSC2aX3ld{BKSzVyIHHmeIVzKDJ2IITvJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? Ml;6NlEzOTZzNUG=
rat RBL2H3 cells MmG3SpVv[3Srb36gZZN{[Xl? NGnDcJVKdmirYnn0bY9vKG:oIFGyN|E5Py2rbnT1Z4VlKGSnZ4LhcpVt[XSrb36gbY4hemG2IGLCUFJJOyClZXzsdy=> NI[zepIyQDN{OEexOi=>
human BJ cells NVXNW491TnWwY4Tpc44h[XO|YYm= MVKyOUDPxE1? NIjhVFQzKGh? MXvBZ5RqfmG2aX;uJI9nKEGtdDDwbI9{eGixconsZZRqd25iYYSgWIhzOzB6IHnuJHBFT0Zvc4TpcZVt[XSnZDDz[ZJ2dSC|dHHyeoVlKGi3bXHuJGJLKGOnbHzzJIF1KDJ3IIXNJJRz\WG2ZXSgNkBpenNiYnXmc5JmKFCGR1[gZ4hidGynbnflJIJ6KGmvbYXuc4Jtd3S2aX7n NHjHeYgyPjd4N{C4OS=>
human HCT116 cells MXnGeY5kfGmxbjDhd5NigQ>? NH64dZkyOCEQvF2= M2DsPVExKG2rbh?= NXnIVJpYUW6qaXLpeIlwdiCxZjDQTVNM[WyyaHGgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KHW|aX7nJHs{OlCfQWTQJIF1KDFyIIXNJIFnfGW{IEGwJI1qdnN? MWqyNlIyOjd{MR?=
human HCT116 cells MnLxSpVv[3Srb36gZZN{[Xl? NGfYfVcyOCEQvF2= NVrLeWxSOTBibXnu NFr0d4RKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDINVA1P1JibYX0ZY51KGmwIHj1cYFvKEiFVEGxOkBk\WyuczD1d4lv\yCdM{LQYWFVWCCjdDCxNEB2VSCjZoTldkAyOCCvaX7z NHTUS4gzOjJzMkeyNS=>
human HCT116 cells MUjGeY5kfGmxbjDhd5NigQ>? MnXBNVAh|ryP NHHWRpVKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCc3RiU3XyOFc{KHCqb4PwbI9zgWyjdHnvckBifCBzMDD1UUBjgSCrbX31co9jdG:2IHHuZYx6e2m| NXzIWZVLOjJ{MUK3NlE>
human HCT116 cells MWPGeY5kfGmxbjDhd5NigQ>? NX:0OVQ6OTBizszN NFzjUo9KdmirYnn0bY9vKG:oIGDJN2tidHCqYTDINVA1P1JibYX0ZY51KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHreEBU\XJ2N{OgdIhwe3Cqb4L5cIF1cW:wIHH0JFExKHWPIHL5JIludXWwb3Lsc5Qh[W6jbInzbZM> NHvUPIczOjJzMkeyNS=>
human PC3 cells M1PZOWZ2dmO2aX;uJIF{e2G7 M3zsdVUxKM7:TR?= NXe5Z5pkQCCq Ml;jTY5lfWO2aX;uJI9nKHB{MTDwdo91\WmwIHX4dJJme3Orb36gbY4hcHWvYX6gVGM{KGOnbHzzJIF1KDVyIIXNJIlv[3WkYYTl[EBnd3JiODDodpMh[nliV3XzeIVzdiCkbH;0eIlv\yC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?= MojpNlI6QTh2N{K=
human LNCAP cells MVHGeY5kfGmxbjDhd5NigQ>? NE[z[noxNjFvM{Cg{txO MWSzNEBucW5? NET5UHZKdmirYnn0bY9vKG:oIFHreEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gUG5ESVBiY3XscJMh[XRiMD6xJJRwKDNyIIXNJIFnfGW{IEOwJI1qdnNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MX6yNlg{OjNzNh?=
human LNCAP cells MlzrSpVv[3Srb36gZZN{[Xl? M2j2flAvOS1|MDFOwG0> M3njVlI1KGh? MoLmSI94dnKnZ4XsZZRqd25ib3[gd5Vzfmm4aX6g[ZhxemW|c3nvckBqdiCqdX3hckBNVkODUDDj[YxteyCjdDCwMlEhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M4rBSFIzQDN{M{G2
HUVEC MkiwSpVv[3Srb36gZZN{[Xl? NWLlRW9JOTBvMkWg{txO M4fFZlE3KGh? MUTJcohq[mm2aX;uJI9nKFSQRnHsdIhiNXO2aX31cIF1\WRiSVPBUU0yKG2UTlGg[ZhxemW|c3nvckBqdiCKVW\FR{BifCBzMDD0c{AzPSC3TTDwdoV1emWjdHXkJIZweiBzNjDodpMh[mWob4LlJHRPTmGucHjhJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjC2JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? NFrlRYkzOjB{NkSxNC=>
HUVEC M4fhcmZ2dmO2aX;uJIF{e2G7 NUPIblNYOTBvMkWg{txO MlrCN|AhdWmw M33r[GlvcGmkaYTpc44hd2ZiVF7GZYxxcGFvc4TpcZVt[XSnZDDoeY1idiCLQ1HNMVEheHKxbX;0[ZIh[WO2aY\peJkh\XiycnXzd4VlKGmwIFjVWmVEKGG|c3Xzd4VlKGG|IHLleIEu\2GuYXP0c5Nq\GG|ZTDhZ5Rqfmm2eTDheEAyOCC2bzCyOUB2VSCycnX0doVifGWmIH\vdkA{OCCvaX7zJIJm\m:{ZTDUUmZidHCqYTDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNTDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 MnrpNlIxOjZ2MUC=
human H1299 cells NV3XO203TnWwY4Tpc44h[XO|YYm= NH33XXAxNjFvM{Cg{txO NV:ycWV4OjRiaB?= NIHuRY5Fd3ewIILl[5Vt[XSrb36gc4Yh[0mDUEKg[ZhxemW|c3nvckBqdiCqdX3hckBJOTJ7OTDj[YxteyCjdDCwMlEhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NEXSZmkzOjZ{MkC2PS=>
human H1299 cells MW\GeY5kfGmxbjDhd5NigQ>? NF3BZmcxNjFvM{Cg{txO NV7jV|htOjRiaB?= NXfpW2xtTG:5bjDy[Yd2dGG2aX;uJI9nKGOLQWCyJIV5eHKnc4Ppc44hcW5iaIXtZY4hUDF{OUmgZ4VtdHNiYYSgNE4yKHSxIEOwJJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhOTByIIXnM41tKFSUQVnMJIFv\CCSSUPLJIlvcGmkaYTvdkBNYTJ7NECwNi=> NU\1Opl7OjJ4MkKwOlk>
human H1299 cells NYHNe|UxTnWwY4Tpc44h[XO|YYm= MWmwMlEuOzBizszN Mlf6NlQhcA>? MorUTY5lfWO2aX;uJI9nKESUNTDlfJBz\XO|aX;uJIlvKGi3bXHuJGgyOjl7IHPlcIx{KGG2IECuNUB1dyB|MDD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M3jmeFIzPjJ{ME[5
human H1299 cells NWHYRYhRTnWwY4Tpc44h[XO|YYm= NGTBVHYxNjFvM{Cg{txO MYGyOEBp NET1OlVKdmS3Y4Tpc44hd2ZiSl7LMY1m\GmjdHXkJGNJV1BiZYjwdoV{e2mxbjDpckBpfW2jbjDINVI6QSClZXzsd{BifCByLkGgeI8hOzBidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MU[yNlYzOjB4OR?=
HEK293 cells NVrvWYlWTnWwY4Tpc44h[XO|YYm= NG\RO|YyNjViaB?= M37hSmRqe3CuYXPlcYVvfCCxZjDbN2heVFOGIH\yc40hcHWvYX6gOWhVPiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGGodHXyJFEvPSCqcoOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmd? Mo\UNlI2OzdzNUO=
human U251HRE cells MWLGeY5kfGmxbjDhd5NigQ>? MoX1TY5pcWKrdHnvckBw\iCqeYDvfIliNWmwZIXj[YQhUEmIMTDhZ5RqfmG2aX;uJIlvKGi3bXHuJHUzPTGKUlWgZ4VtdHNiYomgZ4VtdCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6 NY\mOWhWOTh3MEG2NFE>
human HCT116 cells NXSxNWl4TnWwY4Tpc44h[XO|YYm= NYnKV25MOTBizszN MVLJcohq[mm2aX;uJI9nKEGtdDDlfJBz\XO|aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjdDCxNEB2VSCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz MXOyNlIyOjd{MR?=
Sf9 cells MlrZ[ox2d3Knc3PlcpQheG:uYYLpfoF1cW:wIHHzd4F6 M4fO[WlvcGmkaYTpc44hd2ZiaIXtZY4hWEl|S3fhcY1iKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZpkh\my3b4Lld4NmdnRicH;sZZJqgmG2aX;uJIF{e2G7 MlztNlEyOjF4M{G=
human HCT116 cells MY\Qdo9tcW[ncnH0bY9vKGG|c3H5 M{nRbVczKGh? Mn7XRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhZpNwemKjbnPlJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NUGuPFIh|ryPLh?= MW[yOVY6Ozd6Nx?=
human HCT116 cells MVrDfZRwfG:6aXRCpIF{e2G7 M{DTb|czKGh? MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21Ok4xOSEQvF2u M2HDZ|IyQTR3MkWw
human PC3 cells M4TuUWN6fG:2b4jpZ:Kh[XO|YYm= MYq3NkBp NHnpU5lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME22NU4{PSEQvF2u M3XKRlIzPDhyOEWx
human HuH7 cells NHL1bldRem:uaX\ldoF1cW:wIHHzd4F6 NVrLWGFkPzJiaB?= NX65T4hHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZYJ{d3KkYX7j[UBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPU[3MlE5KM7:TT6= MnnhNlU3QTN5OEe=
human A549 cells NY[3[3NHWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{LpTFczKGh? NHHwRZRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhZpNwemKjbnPlJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;OEKuN|Ih|ryPLh?= Mk\iNlU3QTN5OEe=
human A549 cells MlfMR5l1d3SxeHnjxsBie3OjeR?= NWewRpZSPzJiaB?= NUfNUWxTS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 NHviZXMzOjR6MEi1NS=>
human PC3 cells NIrIWXBRem:uaX\ldoF1cW:wIHHzd4F6 MYeyOUDPxE1? MoTtNVIxKGh? NFniVoNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDheEAzPSC3TTDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTDy[YxifGm4ZTD0c{BFVVOR NGHpRpQyPjZ6MEG1PS=>
human H460 cells M1e5N2Z2dmO2aX;uJIF{e2G7 M4T6dVMxKM7:TR?= M3jJS2lvcGmkaYTpc44hd2ZiUFmzT{BqdiCqdX3hckBJPDZyIHPlcIx{KGG|c3Xzd4VlKGG|IFHreEBxcG:|cHjvdplt[XSrb36gZZQhW2W{IES3N{B2eCC2bzCzNEB2VQ>? M1[3U|E5PTBzNkCx
human IGROV1 cells NWjwXG0zTnWwY4Tpc44h[XO|YYm= NFS1[|QzPCCq MYnD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDJS3JQXjFiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEdzIIDoZZNmKGG2IEGg[o9t\CCLQ{WwJIFnfGW{IEK0JIhzeyCkeTDmcI94KGO7dH;t[ZRzgQ>? NWfiWpZqOjF{MU[xOVE>
human IGROV1 cells M2mxS2Z2dmO2aX;uJIF{e2G7 MlP6NlQhcA>? NHTOdnFE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBKT1KRVkGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFexJJBp[XOnIHH0JFUh\m:uZDDJR|UxKGGodHXyJFI1KGi{czDifUBndG:5IHP5eI9u\XS{eR?= NIXxUG8zOTJzNkG1NS=>
human H4 cells MWfGeY5kfGmxbjDhd5NigQ>? MWeyJIg> M4rIT2Rm[3KnYYPlJIlvKE[\VlWtVmZRMyC4ZYPpZ4xmKGmwdHXud4l1gSCyZYKgZ4VtdCCrbjDoeY1idiCKNDDj[YxteyCjZoTldkAzKGi{czDy[YxifGm4ZTD0c{Bkd262cn;s NHf1eJAyQDB{NEW4OC=>
human MGC803 cells NWPiNHd5TnWwY4Tpc44h[XO|YYm= NVX3S4llOjVizszN NGHnXng1QCCq M1zo[2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJG1ISzhyMzDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRiR{KvUUBxcGG|ZTDheEAzPSC3TTDh[pRmeiB2ODDodpMhfXOrbnegdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDh[pRmeiB2ODDodpMh[nliZnzve{BkgXSxbXX0[ZIhemWuYYTpeoUhfG9iY3;ueJJwdA>? NFfqNVEzPDFzOUi2PS=>

... Click to View More Cell Line Experimental Data

体内研究 体内,LY294002也抑制肿瘤生长和诱导凋亡,尤其作用于LoVo肿瘤, 因此作用于鼠癌性腹膜炎模型具有明显药效。[2] LY294002明显抑制卵巢癌生长和腹水形成。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[4]

+ 展开

激酶实验:

使用重组酶和1 μM ATP,通过辐射实验测定LY294002对PI3K的抑制效果。室温下(24oC)进行激酶反应1小时,然后加入PBS终止反应。通过剂量反应曲线测定IC50值。通过激酶选择性筛选来抑制CK2和GSK3β。加入10 μM ATP,测定LY294002作用于一组激酶的效果。
细胞实验:

[2]

+ 展开
  • Cell lines: 结肠癌细胞系: DLD-1, LoVo, HCT15, 和Colo205
  • Concentrations: 0-50 μM
  • Incubation Time: 0-48小时
  • Method:

    1×105个细胞(每孔100 μL)接种在96孔板上。加入LY294002,重复加三次,在37oC下培养0-48小时。处理后, 10 μL Premix WST-1 加到每孔中,然后在37oC下温育60分钟,然后用微型计数板在450nm处测定吸光值。


    (Only for Reference)
动物实验:

[3]

+ 展开
  • Animal Models: 腹腔注射OVCAR-3细胞的无胸腺裸鼠(5-7周大)
  • Formulation: 溶解在DMSO和0.25 ml PBS中
  • Dosages: 0-100 mg/kg
  • Administration: 每天腹腔注射,持续3周
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 36 mg/mL (117.13 mM)
Ethanol 21 mg/mL (68.32 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 307.34
化学式

C19H17NO3

CAS号 154447-36-6
稳定性 powder
in solvent
别名 SF 1101, NSC 697286

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I want to buy a inhibitor that can inactivates Akt/PI3K. I notice the protocol of LY294002 (catalog no. s1105) says it will inactivates Akt/PKB. Is LY294002 available for Akt/PI3K inhibition?

  • 回答:

    LY294002 is the first synthetic molecule known to inhibit PI3Kα/δ/β with IC50 of 0.5 μM/0.57 μM/0.97 μM, respectively. It can inhibit PI3K/AKT. PKB is the alternative name of AKT.

  • 问题 2:

    What is the suggested formulation of this compound for mouse injection(i.p.)?

  • 回答:

    It can be dissolved in 4% DMSO/30% PEG 300/5% Tween 80/ddH2O at 5 mg/ml clearly, and the concentration of DMSO is safe for in vivo experiments.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID