LY294002

目录号:S1105 别名: SF 1101, NSC 697286

LY294002 Chemical Structure

Molecular Weight(MW): 307.34

LY294002 是首个合成的已知抑制PI3Kα/δ/β的小分子,在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM;在溶液中比在Wortmannin中稳定,也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶,还能够结合一些与PI3K家族无关的新型靶点。

规格 价格 库存 购买数量  
RMB 1570.11 现货
RMB 1188.16 现货
RMB 2196.01 现货
RMB 6298.74 现货
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客户使用Selleck该产品发表文献74篇:

客户使用该产品的15个实验数据:

  • Type I IFNs induce ISG expression in a serine phosphorylation-independent manner. Cells were treated with kinase inhibitors (SB203580, rottlerin, Ly294002, PD98059, and SP600125) before stimulation with IFN-a and IFN-b for 6 hours. Cell lysates were used in western blotting and probed for serine phosphorylation.The relative amounts of pSTAT1 S727 in (up-graph) were quantied and normalized to an untreated control (below-graph).

    Hepatology 2014 59(4), 1262-72. LY294002 purchased from Selleck.

    different signal-inhibitory profile by applying 120 nmol/L BGT226, 20 μmol/L LY294002, or 100 nmol/L rapamycin in FaDu cells.

    Clin Cancer Res 2011 17, 7116-7126. LY294002 purchased from Selleck.

  • Stimulation response of p37d-expressing cells. (c) Western blot of 5 min serum stimulated HEK-293 cells stably expressing p37d, p110d or control, with the presence of inhibitors as indicated at the top.

    Oncogene 2012 31, 3277–3286. LY294002 purchased from Selleck.

    Immunofluorescence and flow cytometric analyses of the translocation of ABCG2 in MCF-7 FLV1000 cells before and after treatment with the PI3K inhibitor LY294002. Confocal microscopy of MCF-7 FLV1000 cells after a 16-h treatment with 2 uM or 4 uM of LY294002 was performed as described in Figure.

    Br J Pharmacol 2013 70(5), 1137-51. LY294002 purchased from Selleck.

  • SCC61 or Detroit 562 xenografted tumors respond to treatment by PI3K inhibitor, LY294002 alone, or in combination with gefitinib. % tumor growth is shown. By two-way ANOVA, the effect of treatment and time is considered significant;

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

    Inhibition of EGFR and PI3K/AKT pathway has additive effects in SCCHN cell lines with constitutively active PIK3CA mutations, while removing negative regulation of PI3K/AKT pathway increases resistance in sensitive SCCHN cell line. A, results from MTT assays on cells treated with PI3K inhibitor, LY294002, and gefitinib.

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

  • Lyn-PI3K-BTK signaling is required for the inhibitory effect of anti-CD180 on the activation of IFN-α signaling. (a and b) Isolated murine splenic B220+ B cells were pretreated with dasatinib (5 μM), a Lyn inhibitor; LY294002 (5 μM), a PI3K inhibitor; ibturinib (1 μM), a BTK inhibitor; enzastaurin (1 μM), a PKC β inhibitor; U0126 (3 μM), an ERK inhibitor; SP600126 (2 μM), a JNK inhibitor or SB20358 (1 μM), a p38 inhibitor for 1 h, followed by stimulation with anti-CD180 antibody (0.2 μg/mL) or mouse IFN-α (1000 U/mL) for 4 h. qPCR analysis of the expression of IFIT1 (a) and MX1 (b). (c) Isolated murine splenic B220+ B cells were pretreated with dasatinib (5 μM), LY294002 (5 μM), and ibturinib (1 μM) for 1 h, followed by stimulation with anti-CD180 antibody (0.2 μg mL−1) or mouse IFN-α (1000 U mL−1) for 40 min. Western blot analysis of the phosphorylation level of STAT-2. The data shown represent the mean values of three independent experiments, and the error bars represent the SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by ANOVA; ns denotes p > 0.05.

    Cell Mol Immunol, 2017, 14(2):192-202. LY294002 purchased from Selleck.

    The effects of GLP-1R siRNA and LY294002 on neurological score at 24 hours after subarachnoid hemorrhage (SAH) in Ex-4 treated rats.

    Neuropharmacology, 2018, 128:142-151. LY294002 purchased from Selleck.

  • Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

    Comparative effects of wortmannin and LY294002 on ADRP and β -casein synthesis. Con fluent HC11 cells were treated according to the standard procedure in the absence or presence of 4 ng/ml EGF either with or without 100 nM wortmannin or 50μM LY294002. Cellular proteins were analyzed by SDS-PAGE and immunoblot successively probed with anti-ADRP, anti-β-casein, anti-β -actin antibodies and their respective HRP-conjugated secondary antibodies. ECL signals were quantified by densitometry and normalized to β-actin. Each experimental condition was performed in triplicate. Percentages of inhibition for both inhibitor and hormonal treatments are indicated in the text.

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

  • Inhibition of PI3K, ERK and mTOR prevents the activation of S6K1 and S6 induced by suppression of PKD1 activity. A549 cells were incubated in the absence (-) or presence of either 5 uM Kb or 5 uM Kb and 20 uM LY294002 or 5 uM Kb and 10 uM BKM120 (as indicated) for 1 h prior to stimulation of cells with 50 nM PMA for 30 min and 1 h.

    Int J Biochem Cell Biol 2015 60, 34-42. LY294002 purchased from Selleck.

    IGF1 increases apical fibronectin in late stage blastocysts. Representative confocal images of blastocysts stained for fibronectin (Green) and DAPI (Blue). Day 5 blastocysts were cultured in SS medium for 24 (A and B), 48 (C and D) or 72 h (E-H) in the absence (A, C and E) or presence (B, D and F) of 10 ng/ml IGF1 or in the presence of a PI3 Kinase inhibitor (5 uM LY294002 + 10 ng/ml IGF1) (G) or in the presence of 10 ng/ml IGF1, attached to a coverslip (H). Scale bars represent 20 mm. Red line indicates location of coverslip. Arrow indicates location of the inner cell mass (ICM). The following total number of blastocysts were analysed, from at least 3 experimental repeats in each treatment; 24 in the SS group (24 h), 21 in SS plus IGF1 (24 h), 26 in SS (48 h), 30 in SS plus IGF1 (48 h), 40 in SS (72 h), 59 in SS plus IGF1 (72 h), 13 in SS plus IGF1 + LY294002 (48 h) and 15 blastocysts were analysed that were attached to a coverslip (72 h).

    Hum Reprod 2015 30(2), 284-98. LY294002 purchased from Selleck.

  • PTEN transfection or inhibition of the PI3K-AKT and IGF-1R signaling pathways increases the trastuzumab sensitivity of NCI-N87/TR cells. After treating the cells with a PI3K inhibitor (LY294002), the expression of AKT and P-AKT proteins was assessed by Western blotting. Tubulin expression indicated equal loading. All gels run under the same experimental conditions and the experiments were repeated 3 times. The representative images were cropped and shown. Control: cells without any treatment.

    Sci Rep 2015 5, 11634. LY294002 purchased from Selleck.

    We treated all of drugs in T47D which has a PI3KCA H1044R mutation with the concentration shown below for 1 hour and performed western blot analysis using antibodies to phospho-AKT(SERINE 472), and total AKT.

     

     

    2010 Saraswati Sukumar of Johns Hopkins University School of Medicine. LY294002 purchased from Selleck.

  • T47Dcells were pretreated with 100 ng/ml EGF for 20 min and then treated with the indicated concentrations of LY294002 for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. LY294002 purchased from Selleck.

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述 LY294002 是首个合成的已知抑制PI3Kα/δ/β的小分子,在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM;在溶液中比在Wortmannin中稳定,也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶,还能够结合一些与PI3K家族无关的新型靶点。
特性 LY294002是选择性的, 可渗透细胞的特定PI3K抑制剂,可作用于酶的ATP结合位点,且抑制自体吞噬的螯合作用。
靶点
p110α [1]
(Cell-free assay)
p110δ [1]
(Cell-free assay)
p110β [1]
(Cell-free assay)
DNA-PK [6]
(Cell-free)
0.5 μM 0.57 μM 0.97 μM 1.4 μM
体外研究

LY294002是一种非特异性抑制剂,除了class I PI3Ks,它同样还能抑制其他类型PI3Ks、mTOR、DNA-PK以及其他蛋白激酶,如CK2和Pim-1[5]。LY294002使Akt/PKB失活,因此抑制细胞增殖和诱导细胞凋亡。 LY294002作用于结肠癌细胞系,使磷酸化Akt (Ser473)的表达下降, 明显抑制生长和诱导凋亡。[2] LY294002作用于肿瘤细胞,诱导明显的核固缩和减少细胞质体积。因此,在体外,LY294002明显抑制卵巢癌细胞增殖。LY294002诱导细胞停在G1期,导致几乎全部的黑色素瘤细胞增殖被抑制,部分MG-63 (骨肉瘤细胞系)增殖被抑制。LY294002作用于细胞周期的效果可用于研究PI3K激活通路和癌细胞周期之间关系。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-231 cells NW\mW4p7S2WubDDpcpZie2mxbjDhd5NigQ>? NHe5TWEzPCCq MYPJcohq[mm2aX;uJI9nKGWyaYTo[Yxq[WxiZ4Lve5RpKG[jY4Tvdk1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNicILlMYlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHPlcIwhcW64YYPpc44h[XO|YYmsJGlEPTB;MD6zPEDPxE1w MXmyNlgxPDFyOB?=
Sf21 cells NF61b45HfW6ldHnvckBie3OjeR?= M4fZfmlvcGmkaYTpc44hd2ZibX;1d4Uhf2muZDD0fZBmKFCLM1vhcJBp[SCneIDy[ZN{\WRiaX6gV4YzOSClZXzsd{Bkdy2neIDy[ZN{cW6pIF6teIVzdWmwYXygTIl{NXSjZ3fl[EBpfW2jbjDwPFViKHW|aX7nJGwu[WyyaHGtdIhwe3CqYYTp[JltcW6xc3n0c4whe3Wkc4TyZZRmKGK7IGTMR{Bj[XOnZDDwbI9{eGixcjDpcYFocW6pLDDJR|UxRTBwNTFOwG0> NIfr[JMzOjV{ME[zNC=>
Sf21 cells MU\GeY5kfGmxbjDhd5NigQ>? NWHLfHFlUW6qaXLpeIlwdiCxZjDtc5V{\SC{ZXPvcYJqdmGwdDDQTVNM[WyyaHGg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|LXnu[oVkfGWmIGPmNlEh[2WubIOsJGlEPTB;MD61JO69VS5? Ml7yNVk4PDh{Nkm=
Sf9 cells NYHVfHB5Tmy3b4Lld4NmdnRicH;sZZJqgmG2aX;uJIF{e2G7 Mny4TY5pcWKrdHnvckBw\iCqdX3hckBRUTONYXzwbIEh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDifUBndHWxcnXzZ4VvfCCyb3zhdol7[XSrb36gZZN{[XluIFnDOVA:OC53NTFOwG0v M3HOTFIyOTJzNkOx
THP1 cells NG\3PYlHfW6ldHnvckBie3OjeR?= NVGxU2czUW6qaXLpeIlwdiCxZjDTR2YyNWmwZIXj[YQhWEuEL1HreEBxcG:|cHjvdplt[XSrb36gbY4hXEiSMTDj[YxteyxiSVO1NF0yNjBzIN88UU4> MlTyNVY4QDl5NEK=
human PC3 cells M3vWXGZ2dmO2aX;uJIF{e2G7 NV\0XG1zOzBibXnu NInzW|NKdmirYnn0bY9vKG:oIGDJN2shcW5iaIXtZY4hWEN|IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJGFMXCCyaH;zdIhwenmuYYTpc44h[XRic3XybY5mKDR5MzDh[pRmeiB|MDDtbY5{KGK7IFXMTXNCNCCLQ{WwQVEvO87:TT6= NWThdXBmOjN2MUCwNFU>
HeLa (human carcinoma) cells. NVvRPZk3TnWwY4Tpc44h[XO|YYm= MlrJTY4hfmm2cn:gbY5pcWKrdHnvckBw\iCGTlGt[IVx\W6mZX70JJBzd3SnaX6gb4lv[XOnKFTORU1RUyliZoLvcUBJ\UyjIDjoeY1idiClYYLjbY5wdWFrIHPlcIx{NCCLQ{WwQVEvPCEQvF2u MWOxOVY2QDh5MB?=
THP1 cells MlfuSpVv[3Srb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKE2FUEGtbY5lfWOnZDDQT2IwSWu2IIDoc5NxcG:{eXzheIlwdiCrbjDUTHAyKGOnbHzzMEBKSzVyPUGuOlUh|ryPLh?= MmPiNVY4QDl5NEK=
HeLa cells MlewSpVv[3Srb36gZZN{[Xl? Ml7vTY5pcWKrdHnvckBw\iCvVF;SJJBzd3SnaX6gbZNwdGG2ZXSg[pJwdSCKZVzhJINmdGy|LDDJR|UxRTJwNTFOwG0v Mn30NVU3PTh6N{C=
human MCF7 cells NUHpUoF{S3m2b4TvfIlkyqCjc4PhfS=> M13RWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMhcW5icILld4Vv[2Vib3[gNlAh|ryPIHPocI9zd3G3aX7lJIJ6KFOUQjDhd5NigSxiR1m1NF0zNjZ|IN88UU4> Mlu1NVg3QTF6OUS=
HeLa cells NIf0XmZHfW6ldHnvckBie3OjeR?= NIHYd4VKdmirYnn0bY9vKG:oIFHYMVc2ODNiYnnu[Ilv\yC2bzDy[YNwdWKrbnHueEBRdGt|IHX4dJJme3OnZDDpckBJ\UyjIHPlcIx{KGK7IGfld5Rmem5iYnzveEwhUUN3PUOg{txONg>? NXXmWYt2OTdzM{WyOFg>
human MCF7 cells MmLyR5l1d3SxeHnjxsBie3OjeR?= M3\NXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMhcW5icILld4Vv[2Vib3[gNVAhfU1iY3jsc5JweXWrbnWgZpkhW1KEIHHzd4F6NCCJSUWwQVMvODhizszNMi=> NEKzUZIyQDZ7MUi5OC=>
human MCF7 cells NHrh[ZJEgXSxdH;4bYPDqGG|c3H5 NIjwWWFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGK7IGPSRkBie3OjeTygS2k:Oy5zNjFOwG0v NUSzTFdSOTh4OUG4PVQ>
human MDA-MB-231 cells MUHDfZRwfG:6aXRCpIF{e2G7 MnrMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDpckBxemW|ZX7j[UBw\iB{MDFOwG0h[2iub4LvdZVqdmViYomgV3JDKGG|c3H5MEBIUT1|LkOyJO69VS5? NX:0dHpHOTh4OUG4PVQ>
human MDA-MB-231 cells MWTDfZRwfG:6aXRCpIF{e2G7 MlXHR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDpckBxemW|ZX7j[UBw\iBzMDD1UUBkcGyxcn;xeYlv\SCkeTDTVmIh[XO|YYmsJGdKPTB;ND6zOUDPxE1w M2mxZVE5PjlzOEm0
human MDA-MB-468 cells MVTDfZRwfG:6aXRCpIF{e2G7 NELQRpVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFY5KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIEKwJJVOKGOqbH;yc5F2cW6nIHL5JHNTSiCjc4Phfg+9lCCLQ{WwQVQvPzZizszNMi=> NHrFd|EyQDZ7MUi5OC=>
human HCT116 cells NV\IdoRNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmPWOFghcA>? MYPHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMhd3[ncnX4dJJme3OrbnegVGk{U2GucHjhJGgyODR5UjDteZRidnRiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF02NjNizszN NUHrfnZOOjJ{MUK3NlE>
human HCT116 cells MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4XtRVQ5KGh? MWHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21Mlgh|ryPLh?= NU\2R3JsOjJ{MUK3NlE>
HeLa cells NVLtRmF4SmmwZHnu[{Bi\m[rbnn0fUBie3OjeR?= NUfxOG01SmmwZHnu[{Bi\m[rbnn0fUBnd3JiUFmzMYtqdmG|ZTDpd49t[XSnZDDmdo9uKEinTHGgZ4VtdHNuIFvpQVYh|ryP M3PkeFE2PjV6OEew
human HCT116 cells MoLVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnLwOFghcA>? Mm\hS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJHBKO0ujbIDoZUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPU[uO{DPxE1w NFj6eZozOjJzMkeyNS=>
MDA-MB-231 cells NVnlSWxKS3m2b4TvfIlkyqCjc4PhfS=> Mn\lR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9Ok44OSEQvF2u NFLJV3EyQDZ7MUi5OC=>
Sf9 cells M{f4SWZ2dmO2aX;uJIF{e2G7 NWf3NVdOUW6qaXLpeIlwdiCxZjDDT|Ih\XiycnXzd4VlKGmwIGPmPUBk\WyuczygTWM2OD14Lkmg{txONg>? MmDHNVg{OjF5MU[=
human U87MG cells NV\VZmd4TnWwY4Tpc44h[XO|YYm= MnfBTY5pcWKrdHnvckBw\iCJU1uzMYJmfGFiaX6gbJVu[W5iVUi3UWch[2WubIOgZpkhTUyLU1GsJGlEPTB;OD6xJO69VS5? Mm\HNVg{PDV4MEm=
human MDA-MB-468 cells M1XEeWN6fG:2b4jpZ:Kh[XO|YYm= M4\ud2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj20Olgh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiMUCgeW0h[2iub4LvdZVqdmViYomgV3JDKGG|c3H5MEBIUTVyPUiuNkDPxE1w NFfXV2YyQDZ7MUi5OC=>
human A375 cells NHjKbppRem:uaX\ldoF1cW:wIHHzd4F6 M{n5flQ3KGh? MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF|N{WgZ4VtdHNiYX\0[ZIhPDZiaILzMEBKSzVyPUiuOEDPxE1w NULtbZB{OTdyNEmyOFg>
human A375 cells NHvreG5Rem:uaX\ldoF1cW:wIHHzd4F6 M{XnTGlvcGmkaYTpc44hd2Zic3XyeY0ucW6mdXPl[EBxem:uaX\ldoF1cW:wIH;mJIh2dWGwIFGzO|Uh[2WubIOsJGlEPTB;OD60JO69VS5? MYSxO|YxOTd|OR?=
human HL60 cells M2TDTWN6fG:2b4jpZ:Kh[XO|YYm= NUHBT2dvPzJiaB?= M3freWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME25Mlk1KM7:TT6= M1zLSVIzPDhyOEWx
mouse Raw264 macrophage NYOyboJpTnWwY4Tpc44h[XO|YYm= MWTJcohq[mm2aX;uJI9nKEN3YT3t[YRq[XSnZDDQT2IwSWu2IIDoc5NxcG:{eXzheIlwdiCrbjDtc5V{\SCUYYeyOlQhdWGlcn;wbIFo\SxiSVO1NF0yOCEQvF2u MV:xOlc5QTd2Mh?=
Sf9 cells NX;ENoVJTnWwY4Tpc44h[XO|YYm= NH71cI1KdmirYnn0bY9vKG:oIFjpd{11[WepZXSgZo93cW6nIGDJN2sh\XiycnXzd4VlKGmwIGPmPUBk\WyuczygTWM2OD1zMDFOwG0v NW\vXGdtOTB7OUizOVE>
human MDA-MB-468 cells M4DWNGN6fG:2b4jpZ:Kh[XO|YYm= NW\RUlN5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVA:OTBwNDFOwG0v MoL4NVg3QTF6OUS=
human PC3 cell NH3BR|ZRem:uaX\ldoF1cW:wIHHzd4F6 NHHafFc4OiCq NXeyNlU4UW6qaXLpeIlwdiCxZjDoeY1idiCSQ{OgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDzdIVkfHKxcHjveI9u\XS{aXOgZY5idHm|aYOsJGlEPTB;MUKuNUDPxE1w MkjkNlM1OTByMEW=
human HL60 cells MorkVJJwdGmoZYLheIlwdiCjc4PhfS=> M4fUfFczKGh? MkDjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYXLzc5Jj[W6lZTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF6LkSzJO69VS5? MoHGNlU3QTN5OEe=
human BT474 cell MlzYVJJwdGmoZYLheIlwdiCjc4PhfS=> MUi3NkBp NEPMdZBKdmirYnn0bY9vKG:oIHj1cYFvKEKWNEe0JINmdGxicILvcIln\XKjdHnvckBi\nSncjC3NkBpenNiYomgd5Bm[3S{b4Doc5RwdWW2cnnjJIFv[Wy7c3nz89yNKEmFNUC9NlAvPyEQvF2u MnPZNlM1OTByMEW=
THP1 cells MnPNSpVv[3Srb36gZZN{[Xl? MUnJcohq[mm2aX;uJI9nKE2FUEGtbY5lfWOnZDDjbIVud3SjeHnzJI9nKFSKUEGgZ4VtdHNuIFnDOVA:OzdizszNMi=> NInsdlMyPjd6OUe0Ni=>
human 184B5 cells M{flXmN6fG:2b4jpZ:Kh[XO|YYm= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCxPFRDPSClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OzlwM{eg{txONg>? NV3WXHl2OTh4OUG4PVQ>
human KB cells NF;rcWtEgXSxdH;4bYPDqGG|c3H5 M1zUZlczKGh? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTR2Lke2JO69VS5? M1jLXlIyQTR3MkWw
mouse RAW264.7 cells M131SGZ2dmO2aX;uJIF{e2G7 NE\WdpAzPSEQvF2= M2fUNlIxKGh? NXj0WoV1UW6qaXLpeIlwdiCxZjDQTVNMN0GNVDDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIHnOU3MheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEK1JO69VSCjZoTldkAzOCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| Mmj5NlQzQTl4MU[=
human A2780 cells M3LIPGFxd3C2b4Ppd{Bie3OjeR?= NFj4WoYzKM7:TR?= NXjSXGp1OTJiaB?= NG\HR5BKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJINqe3CuYYTpck1z\XOrc4ThcpQhcHWvYX6gRVI4QDBiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gdIhwe3Cqb4L5cIF1\WRiQXv0JIxmfmWuIHH0JFIhfU1iYX\0[ZIhOTJiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXNw NFHBXFgyPzZ6NECxPC=>
human SW480 cells MXjGeY5kfGmxbjDhd5NigQ>? NUfHbGhQOjBizszN MmPlNlQhcA>? NIHL[lNF\WO{ZXHz[UBqdiC|dYL2bZZqdiCneIDy[ZN{cW:wIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF1KDJyIIXNJIFnfGW{IEK0JIhzeyCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz M2W0XlE4PDd{OU[y
mouse mast cells MVnGeY5kfGmxbjDhd5NigQ>? NXnrOFB1OjBizszN NFLXSmZKdmirYnn0bY9vKG:oIGPDSk1qdmS3Y3XkJHBMSi:Da4SgdIhwe3Cqb4L5cIF1cW:wIHnuJI1wfXOnIH3hd5Qh[2WubIOgZZQhOjBizszN MXexOlc5QTd2Mh?=
human BJ cells M2DCS2Z2dmO2aX;uJIF{e2G7 M3jIW|I2KM7:TR?= MmDzNkBp NEi1OppKdmirYnn0bY9vKG:oIGDJN2she2mpbnHsbY5oKGmwIGDES2Yue3SrbYXsZZRm\CC|ZYL1cUB{fGG{dnXkJIh2dWGwIFLKJINmdGy|IITyZY5{\mWldHXkJJdqfGhicHzhd41q\CCHR1\QM3BJKGSxbXHpckBw\iCDa4SgZZN{\XO|ZXSgZZMhe3WycILld5Nqd25ib3[gVGguTUeIUDDy[YRqe3S{aXL1eIlwdiC2bzDwcIF{dWFibXXtZpJidmViYYSgNlUhfU1idILlZZRm\CB{IHjyd{Bj\W[xcnWgVGRITiClaHHscIVv\2ViYomgZ49v\m:lYXygcYlkem:|Y3;wfS=> NHrlXYwyPjd4N{C4OS=>
human IGROV1 cells MmjOSpVv[3Srb36gZZN{[Xl? NXLGWpM4OjRxNEigbC=> Mn\yTY5pcWKrdHnvckBw\iCSSUPLM2FsfCCrbjDoeY1idiCLR2LPWlEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5icHjvd5Bpd3K7bHH0[YQhSUuWIHzleoVtKGG2IEGgeI8hPSC2aX3ld{BKSzVyIHHmeIVzKDJ2IITvJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? M1:5ZlIyOjF4MUWx
human IGROV1 cells NWr3ZnVYTnWwY4Tpc44h[XO|YYm= M4jXVlI1NzR6IHi= M4[weWlvcGmkaYTpc44hd2ZiUFmzT{9Cc3RiaX6gbJVu[W5iSVfSU3YyKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHP5Z4xqdiCGMTDs[ZZmdCCjdDCxJJRwKDVidHnt[ZMhUUN3MDDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M3\yTVIyOjF4MUWx
rat RBL2H3 cells MoHFSpVv[3Srb36gZZN{[Xl? NFPVNJZKdmirYnn0bY9vKG:oIFGyN|E5Py2rbnT1Z4VlKGSnZ4LhcpVt[XSrb36gbY4hemG2IGLCUFJJOyClZXzsdy=> MV2xPFMzQDdzNh?=
human BJ cells MUPGeY5kfGmxbjDhd5NigQ>? NFnSVGozPSEQvF2= NHfFdYEzKGh? MlTpRYN1cX[jdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIHH0JHRpejNyODDpckBRTEeILYP0bY12dGG2ZXSgd4VzfW1ic4ThdpZm\CCqdX3hckBDUiClZXzsd{BifCB{NTD1UUB1emWjdHXkJFIhcHK|IHLl[o9z\SCSRFfGJINp[WyuZX7n[UBjgSCrbX31co9jdG:2dHnu[y=> MnG5NVY4PjdyOEW=
human HCT116 cells MWjGeY5kfGmxbjDhd5NigQ>? MofaNVAh|ryP NXzHUJRjOTBibXnu NUXiXlFVUW6qaXLpeIlwdiCxZjDQTVNM[WyyaHGgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KHW|aX7nJHs{OlCfQWTQJIF1KDFyIIXNJIFnfGW{IEGwJI1qdnN? NHnVOG4zOjJzMkeyNS=>
human HCT116 cells M2L4SmZ2dmO2aX;uJIF{e2G7 M4X5TVExKM7:TR?= MYKxNEBucW5? M1rkdGlvcGmkaYTpc44hd2ZiUFmzT4FteGijIFixNFQ4WiCvdYThcpQhcW5iaIXtZY4hUEOWMUG2JINmdGy|IIXzbY5oKFt|MmDdRXRRKGG2IEGwJJVOKGGodHXyJFExKG2rboO= NXPaSXN7OjJ{MUK3NlE>
human HCT116 cells MXPGeY5kfGmxbjDhd5NigQ>? M4G4Z|ExKM7:TR?= MmHPTY5pcWKrdHnvckBw\iCSSUPLZYxxcGFiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gRYt1KFOnckS3N{BxcG:|cHjvdplt[XSrb36gZZQhOTBidV2gZpkhcW2vdX7vZoxwfCCjbnHsfZNqew>? M3X2[VIzOjF{N{Kx
human HCT116 cells NF3nOGlHfW6ldHnvckBie3OjeR?= MnvUNVAh|ryP NFnsWYNKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDINVA1P1JibYX0ZY51KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHreEBU\XJ2N{OgdIhwe3Cqb4L5cIF1cW:wIHH0JFExKHWPIHL5JIludXWwb3Lsc5Qh[W6jbInzbZM> MW[yNlIyOjd{MR?=
human PC3 cells MWnGeY5kfGmxbjDhd5NigQ>? NGHLXpE2OCEQvF2= NHTwcmo5KGh? MXrJcoR2[3Srb36gc4YheDJzIIDyc5RmcW5iZYjwdoV{e2mxbjDpckBpfW2jbjDQR|Mh[2WubIOgZZQhPTBidV2gbY5kfWKjdHXkJIZweiB6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u MY[yNlk6QDR5Mh?=
human LNCAP cells NWrGWHp2TnWwY4Tpc44h[XO|YYm= MmnVNE4yNTNyIN88US=> NVvXZmx4OzBibXnu Mn3aTY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF1KDBwMTD0c{A{OCC3TTDh[pRmeiB|MDDtbY5{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NFHQU2czOjh|MkOxOi=>
human LNCAP cells MXTGeY5kfGmxbjDhd5NigQ>? NITOXooxNjFvM{Cg{txO M3TUelI1KGh? Ml;qSI94dnKnZ4XsZZRqd25ib3[gd5Vzfmm4aX6g[ZhxemW|c3nvckBqdiCqdX3hckBNVkODUDDj[YxteyCjdDCwMlEhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NWTNb2pWOjJ6M{KzNVY>
HUVEC MYDGeY5kfGmxbjDhd5NigQ>? NW\UR44yOTBvMkWg{txO NH\scoEyPiCq M1iwbGlvcGmkaYTpc44hd2ZiVF7GZYxxcGFvc4TpcZVt[XSnZDDJR2FONTFibWLORUBmgHC{ZYPzbY9vKGmwIFjVWmVEKGG2IEGwJJRwKDJ3IIXNJJBz\XS{ZXH0[YQh\m:{IEG2JIhzeyCkZX\vdoUhXE6IYXzwbIEh[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFYhcHK|IHL5JHJVNVCFUjDhcoFtgXOrcx?= Mn\pNlIxOjZ2MUC=
HUVEC MUXGeY5kfGmxbjDhd5NigQ>? NFq5U4gyOC1{NTFOwG0> NF7F[W8{OCCvaX6= NWjobZNTUW6qaXLpeIlwdiCxZjDUUmZidHCqYT3zeIlufWyjdHXkJIh2dWGwIFnDRW0uOSCycn;tc5RmeiCjY4Tpeol1gSCneIDy[ZN{\WRiaX6gTHVXTUNiYYPz[ZN{\WRiYYOgZoV1[S2pYXzhZ5Rwe2mmYYPlJIFkfGm4aYT5JIF1KDFyIITvJFI2KHWPIIDy[ZRz\WG2ZXSg[o9zKDNyIH3pcpMh[mWob4LlJHRPTmGucHjhJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjC1JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> MXKyNlAzPjRzMB?=
human H1299 cells M2O4SGZ2dmO2aX;uJIF{e2G7 MkDKNE4yNTNyIN88US=> NH;qUlIzPCCq MkDLSI94diC{ZXf1cIF1cW:wIH;mJINKSVB{IHX4dJJme3Orb36gbY4hcHWvYX6gTFEzQTliY3XscJMh[XRiMD6xJJRwKDNyIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NUi0UHhSOjJ4MkKwOlk>
human H1299 cells NUXYRlBWTnWwY4Tpc44h[XO|YYm= NWL5RWlLOC5zLUOwJO69VQ>? NH3TXHYzPCCq NEHlOFFFd3ewIILl[5Vt[XSrb36gc4Yh[0mDUEKg[ZhxemW|c3nvckBqdiCqdX3hckBJOTJ7OTDj[YxteyCjdDCwMlEhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kAyODBidXevcYwhXFKDSVygZY5lKFCLM1ugbY5pcWKrdH;yJGx[Ojl2MECy NH7aSpozOjZ{MkC2PS=>
human H1299 cells M2j3UGZ2dmO2aX;uJIF{e2G7 M4rwTVAvOS1|MDFOwG0> MmrFNlQhcA>? MoTVTY5lfWO2aX;uJI9nKESUNTDlfJBz\XO|aX;uJIlvKGi3bXHuJGgyOjl7IHPlcIx{KGG2IECuNUB1dyB|MDD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M3vyR|IzPjJ{ME[5
human H1299 cells MonySpVv[3Srb36gZZN{[Xl? M4DWblAvOS1|MDFOwG0> MUCyOEBp NH;4d21KdmS3Y4Tpc44hd2ZiSl7LMY1m\GmjdHXkJGNJV1BiZYjwdoV{e2mxbjDpckBpfW2jbjDINVI6QSClZXzsd{BifCByLkGgeI8hOzBidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NVG0emQ5OjJ4MkKwOlk>
HEK293 cells M{PLTWZ2dmO2aX;uJIF{e2G7 MnThNU42KGh? NIDkOZhFcXOybHHj[Y1mdnRib3[gX|NJZUyVRDDmdo9uKGi3bXHuJFVJXDZicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyNjViaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6p MknTNlI2OzdzNUO=
human U251HRE cells NVPqfoVkTnWwY4Tpc44h[XO|YYm= NUfkd49kUW6qaXLpeIlwdiCxZjDofZBwgGmjLXnu[JVk\WRiSFnGNUBi[3SrdnH0bY9vKGmwIHj1cYFvKFV{NUHIVmUh[2WubIOgZpkh[2WubDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7 NH7SeJUyQDVyMU[wNS=>
human HCT116 cells NUXIcJczTnWwY4Tpc44h[XO|YYm= MXOxNEDPxE1? NVThRXJzUW6qaXLpeIlwdiCxZjDBb5Qh\XiycnXzd4lwdiCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZQhOTBidV2gZpkhcW2vdX7vZoxwfCCjbnHsfZNqew>? MlK4NlIzOTJ5MkG=
Sf9 cells M33ufIZtfW:{ZYPj[Y51KHCxbHHybZpifGmxbjDhd5NigQ>? NI\hcpVKdmirYnn0bY9vKG:oIHj1cYFvKFCLM1vnZY1u[SCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIJ6KG[udX;y[ZNk\W62IIDvcIFzcXqjdHnvckBie3OjeR?= MVyyNVEzOTZ|MR?=
human HCT116 cells MYjQdo9tcW[ncnH0bY9vKGG|c3H5 NVPtXm1ZPzJiaB?= NWnSbJlxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBi[nOxcnLhcoNmKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OVEvQDJizszNMi=> NWO5dlltOjV4OUO3PFc>
human HCT116 cells M3PFfGN6fG:2b4jpZ:Kh[XO|YYm= NYq5R5c5PzJiaB?= NVPZPGJlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PTZwMEGg{txONg>? M2HpRlIyQTR3MkWw
human PC3 cells NVrzV2NqS3m2b4TvfIlkyqCjc4PhfS=> MYe3NkBp MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD14MT6zOUDPxE1w MXuyNlQ5ODh3MR?=
human HuH7 cells M4jze3Bzd2yrZnXyZZRqd25iYYPzZZk> NEToPIo4OiCq MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjYoPvdoJidmOnIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PjdwMUig{txONg>? M1rFSFI2Pjl|N{i3
human A549 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 Mn65O|IhcA>? MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjYoPvdoJidmOnIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QDJwM{Kg{txONg>? MmXoNlU3QTN5OEe=
human A549 cells MXTDfZRwfG:6aXRCpIF{e2G7 Mn33O|IhcA>? NVfocWFGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 M3rXUFIzPDhyOEWx
human PC3 cells MVLQdo9tcW[ncnH0bY9vKGG|c3H5 M4XJXFI2KM7:TR?= NIOwbIsyOjBiaB?= MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjdDCyOUB2VSCjZoTldkAyOjBiaILzJIJ6KE2WVDDhd5NigSC{ZXzheIl3\SC2bzDEUXNQ MU[xOlY5ODF3OR?=
human H460 cells MXrGeY5kfGmxbjDhd5NigQ>? NFvofFE{OCEQvF2= M{HkVmlvcGmkaYTpc44hd2ZiUFmzT{BqdiCqdX3hckBJPDZyIHPlcIx{KGG|c3Xzd4VlKGG|IFHreEBxcG:|cHjvdplt[XSrb36gZZQhW2W{IES3N{B2eCC2bzCzNEB2VQ>? MlLBNVg2ODF4MEG=
human IGROV1 cells NVnq[HY6TnWwY4Tpc44h[XO|YYm= M1\IWVI1KGh? NFLER5VE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBKT1KRVkGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFexJJBp[XOnIHH0JFEh\m:uZDDJR|UxKGGodHXyJFI1KGi{czDifUBndG:5IHP5eI9u\XS{eR?= MX[yNVIyPjF3MR?=
human IGROV1 cells NHXM[YNHfW6ldHnvckBie3OjeR?= M4jIcVI1KGh? M{Lwb2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGlIWk:YMTDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRiR{GgdIhie2ViYYSgOUBnd2ymIFnDOVAh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 MYqyNVIyPjF3MR?=
human H4 cells MorESpVv[3Srb36gZZN{[Xl? M4rHe|IhcA>? NILxfJRF\WO{ZXHz[UBqdiCIWW\FMXJHWCtidnXzbYNt\SCrboTlcpNqfHlicHXyJINmdGxiaX6gbJVu[W5iSESgZ4VtdHNiYX\0[ZIhOiCqcoOgdoVt[XSrdnWgeI8h[2:wdILvcC=> MnO4NVgxOjR3OES=
human MGC803 cells MWDGeY5kfGmxbjDhd5NigQ>? MXuyOUDPxE1? MV60PEBp NYD0V41US2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hVUeFOECzJINmdGy|IHHzd4V{e2WmIHHzJIFk[3WvdXzheIlwdiCjdDDHNk9OKHCqYYPlJIF1KDJ3IIXNJIFnfGW{IES4JIhzeyC3c3nu[{Bxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIFnfGW{IES4JIhzeyCkeTDmcI94KGO7dH;t[ZRmeiC{ZXzheIl3\SC2bzDjc451em:u M1TBXVI1OTF7OE[5

... Click to View More Cell Line Experimental Data

体内研究 体内,LY294002也抑制肿瘤生长和诱导凋亡,尤其作用于LoVo肿瘤, 因此作用于鼠癌性腹膜炎模型具有明显药效。[2] LY294002明显抑制卵巢癌生长和腹水形成。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[4]

+ 展开

激酶实验:

使用重组酶和1 μM ATP,通过辐射实验测定LY294002对PI3K的抑制效果。室温下(24oC)进行激酶反应1小时,然后加入PBS终止反应。通过剂量反应曲线测定IC50值。通过激酶选择性筛选来抑制CK2和GSK3β。加入10 μM ATP,测定LY294002作用于一组激酶的效果。
细胞实验:

[2]

+ 展开
  • Cell lines: 结肠癌细胞系: DLD-1, LoVo, HCT15, 和Colo205
  • Concentrations: 0-50 μM
  • Incubation Time: 0-48小时
  • Method:

    1×105个细胞(每孔100 μL)接种在96孔板上。加入LY294002,重复加三次,在37oC下培养0-48小时。处理后, 10 μL Premix WST-1 加到每孔中,然后在37oC下温育60分钟,然后用微型计数板在450nm处测定吸光值。


    (Only for Reference)
动物实验:

[3]

+ 展开
  • Animal Models: 腹腔注射OVCAR-3细胞的无胸腺裸鼠(5-7周大)
  • Formulation: 溶解在DMSO和0.25 ml PBS中
  • Dosages: 0-100 mg/kg
  • Administration: 每天腹腔注射,持续3周
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 36 mg/mL (117.13 mM)
Ethanol 21 mg/mL (68.32 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 307.34
化学式

C19H17NO3

CAS号 154447-36-6
稳定性 powder
in solvent
别名 SF 1101, NSC 697286

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I want to buy a inhibitor that can inactivates Akt/PI3K. I notice the protocol of LY294002 (catalog no. s1105) says it will inactivates Akt/PKB. Is LY294002 available for Akt/PI3K inhibition?

  • 回答:

    LY294002 is the first synthetic molecule known to inhibit PI3Kα/δ/β with IC50 of 0.5 μM/0.57 μM/0.97 μM, respectively. It can inhibit PI3K/AKT. PKB is the alternative name of AKT.

  • 问题 2:

    What is the suggested formulation of this compound for mouse injection(i.p.)?

  • 回答:

    It can be dissolved in 4% DMSO/30% PEG 300/5% Tween 80/ddH2O at 5 mg/ml clearly, and the concentration of DMSO is safe for in vivo experiments.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID