Idelalisib (CAL-101, GS-1101)

For research use only. Not for use in humans.

目录号：S2226 中文名称：艾代拉里斯，艾代拉利司

Idelalisib (CAL-101, GS-1101) Chemical Structure

CAS No. 870281-82-6

Idelalisib (CAL-101, GS-1101) 是选择性p110δ抑制剂，在无细胞试验中IC50为 2.5 nM；对 p110δ 表现出的选择性是对 p110α/β/γ 的 40 到 300 倍，对p110δ的选择性是对 C2β，hVPS34，DNA-PK 和 mTOR的400到4000倍。Idelalisib 还可诱导自噬。

规格

价格

库存

购买数量

10mM (1mL in DMSO)	RMB 1279.19	现货
10mg	RMB 972.85	现货
50mg	RMB 3867.24	现货
200mg	RMB 10401.3	现货
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客户使用Selleck生产的Idelalisib (CAL-101, GS-1101)发表文献201篇：

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产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述

特性

Calistoga 暗示 CAL-101治疗血液恶性肿瘤可能有更广泛的应用价值。

靶点

p110δ ^[1] (Cell-free assay)	p110γ ^[1] (Cell-free assay)
2.5 nM	89 nM

体外研究

CAL-101 对p110α, p110β,和p110γ作用效果不大。CAL-101作用于原代嗜碱细胞特定阻断FcϵR1 p110δ调节的 CD63表达，EC50 为8 nM。与急性髓性白血病(AML) 和骨髓增生性肿瘤(MPN) 细胞相比，CAL-101 作用于B-cell急性淋巴细胞白血病(B-ALL)和慢性淋巴细胞白血病(CLL) 细胞时显示更强的活性。CAL-101 作用于SU-DHL-5, KARPAS-422 和CCRF-SB细胞，降低pAktS473, pAktT308, 和下游靶点S6， EC50为0.1到1.0 μM。 ^[1] CAL-101 作用于CLL细胞，诱导选择性细胞毒性，不是通过突变状态或间期细胞遗传学,主要通过caspase依赖机制。与正常B细胞相比，CAL-101作用于CLL 细胞优先产生细胞毒性，和LY294002相比，作用于其他造血细胞不会产生毒性。CAL-101 作用于T 细胞和天然杀伤细胞缺乏直接的细胞毒性潜能。CAL-101抑制炎症细胞因子的产生,比如 IL-6, IL-10, TNF-α,和IFN-γ,且激活诱导的细胞因子,如CD40L。CAL-101 也抗CD40L调节的CLL细胞存活。^[2] CAL-101 作用于L1236和L591细胞, 诱导细胞在G1期积累，在S期下降，说明 CAL-101可以作为治疗霍杰金淋巴瘤(HL)的一种新策略。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
MEC1	MkPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NV\SRXlWTE2VTx?=	NFfrUpZKSzVyPUKwMlQh\|ryP	NF7T[Jo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm5PVM2Oid-MkW5PVk{PTJ:L3G+
CLL PBMCs	Mof1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MknsSG1UVw>?	MV;JR\|UxRTJwOTDuUS=>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTlzN{K2O{c,OjV7MUeyOlc9N2F-
U266	M4fo[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWm0NEDPxE1?	MmH1OFghcA>?		NX[zR5c3PzlwNTWgbY5pcWKrdHnvckBz[XSn	NGfRO\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzPVM{Oid-MkWzN\|k{OzJ:L3G+
K562	M2fWd2Z2dmO2aX;uJGF{e2G7	NIjDcIgyKM7:TR?=	MVezJIg>		MWDJcohq[mm2aX;uJI9nKEGtdDDwbI9{eGixconsZZRqd25?	MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUS3O\|UoRjJ3MEG0O\|c2RC:jPh?=
K562	MWTGeY5kfGmxbjDBd5NigQ>?	MWexJO69VQ>?	M4\6eVMhcA>?		NHHX[ZJKdmirYnn0bY9vKG:oIGC3NHM3UyCyaH;zdIhwenmuYYTpc44>	MoriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUS3O\|UoRjJ3MEG0O\|c2RC:jPh?=
K562	NHf4fXpHfW6ldHnvckBCe3OjeR?=	M{TmSlEh\|ryP	MXqzJIg>		MWnJcohq[mm2aX;uJI9nKEeVS{OgdIhwe3Cqb4L5cIF1cW:w	M{\rcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEG0O\|c2Lz5{NUCxOFc4PTxxYU6=
K562	NYPLT2RqT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2PBe\|Eh\|ryP	MWG3NkBp		M4TlS2lvcGmkaYTpc44hd2ZicILvcIln\XKjdHnvci=>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzNEe3OUc,OjVyMUS3O\|U9N2F-
Primary AML cell	MYXGeY5kfGmxbjDBd5NigQ>?	NYLZOJhUOSEQvF2=	MkLqN{Bp		Moq1TY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:w	M3i3VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEG0O\|c2Lz5{NUCxOFc4PTxxYU6=
Primary AML cell	M1HwSmZ2dmO2aX;uJGF{e2G7	MWGxJO69VQ>?	NXPwTGlQOyCq		MVTJcohq[mm2aX;uJI9nKFB5MGO2T{BxcG:\|cHjvdplt[XSrb36=	M3XUbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEG0O\|c2Lz5{NUCxOFc4PTxxYU6=
Primary AML cell	M2PGTWZ2dmO2aX;uJGF{e2G7	NV[wNIxGOSEQvF2=	MUCzJIg>		NW\4WJVnUW6qaXLpeIlwdiCxZjDHV2s{KHCqb4PwbI9zgWyjdHnvci=>	NITibnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxOFc4PSd-MkWwNVQ4PzV:L3G+
Primary AML cell	Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1eyWVEh\|ryP	MlHuN{Bp		MVnTeZBxemW\|c3nvckBw\iC{Ul7BJJN6dnSqZYPpdy=>	Mnm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUS3O\|UoRjJ3MEG0O\|c2RC:jPh?=
Microglia	M4HoPGZ2dmO2aX;uJGF{e2G7	M3y0dVUh\|ryP	NGPKbpEyOCCq	M{DUSmROW09?	MXvE[YNz\WG\|ZTDv[kBVVk[jIIPlZ5JmfGmxbjDmdo9uKEySUz3zeIlufWyjdHXkJEBxOTFyzsTEPVExSS:GOUGwRUBucWO{b3fsbYE>	NUjPN5h4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlU3QDRpPkK0OlI2Pjh2PD;hQi=>
Primary CLL cell	NVzqTpBITnWwY4Tpc44hSXO\|YYm=	NGrjO4gyKM7:TR?=	MmHrNVUhdWmw	NGHLPGtFVVOR	NXz3UVdtSmyxY3vzJGJEWi2rbnT1Z4VlKEyFUEGgd4VzcW6nLUWgZYN1cX[jdHnvci=>	NWjCT4dIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNFkzOzNpPkK0NFA6OjN\|PD;hQi=>
JEKO-1	NEHlU5VHfW6ldHnvckBCe3OjeR?=	NVLrWmltOSEQvF2=	MUi3NkBp		MVXJcohq[mm2aX;uJI9nKEGtdDDwbI9{eGixconsZZRqd25iaX6gTYdONXO2aX31cIF1\WRiSlXLU{0y	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2MUW0NUc,OjN\|NEG1OFE9N2F-
Granta-519	MoW1SpVv[3Srb36gRZN{[Xl?	NIjiUVYyKM7:TR?=	MlLoNkBp		NHvwTmtKdmirYnn0bY9vKG:oIFHreEh1OzB6KTDwbI9{eGixconsZZRqd25?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2MUW0NUc,OjN\|NEG1OFE9N2F-
Granta-519	M4HiUmZ2dmO2aX;uJGF{e2G7	NW\iPG5HOSEQvF2=	NVrQTWRVOiCq		MX3Jcohq[mm2aX;uJI9nKEGtdDjzOFc{MSCyaH;zdIhwenmuYYTpc44>	NVrnUXpPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOFE2PDFpPkKzN\|QyPTRzPD;hQi=>
JEKO-1	NXq2bII1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M334SlExKM7:TR?=	MoLkO\|IhcA>?		NW\uNXdpUW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9vKHOuaXfoeIx6	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2MUW0NUc,OjN\|NEG1OFE9N2F-
JEKO-1	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYS2XVF3PSEQvF2=	M1P2clczKGh?		NIfzeFBld2W\|IH7veEBqdmS3Y3WgZ4VtdCCleXPs[UBienKnc4Sgc5Ih[XCxcITvd4l{	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ5NkKyNEc,OjN4N{[yNlA9N2F-
MAVER-1	NED0TGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MnH6OUDPxE1?	M{PQWVczKGh?		MX\kc4V{KG6xdDDpcoR2[2ViY3XscEBkgWOuZTDhdpJme3Rib4KgZZBweHSxc3nz	M{fzR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Nke2NlIxLz5{M{[3OlIzODxxYU6=
MINO	NIjw[m1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M{jRW\|Uh\|ryP	MonUO\|IhcA>?		M1vpdYRw\XNibn;0JIlv\HWlZTDj[YxtKGO7Y3zlJIFzemW\|dDDvdkBieG:ydH;zbZM>	MkfUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{[yNlAoRjJ\|Nke2NlIxRC:jPh?=
SP53	MmLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGjofmwxNjFizszN	MoDRO\|IhcA>?		MoWx[I9meyCwb4SgbY5lfWOnIHPlcIwh[3mlbHWgZZJz\XO2IH;yJIFxd3C2b4Ppdy=>	MnnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{[yNlAoRjJ\|Nke2NlIxRC:jPh?=
HH	MlTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVvlZmNyOTBizszN	MX23NkBp	NULVcGlyTE2VTx?=	MkPETY5lfWO2aX;uJI9nKGGyb4D0c5NqeyC\|bHnnbJRtgQ>?	M{LjTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OECxPVU6Lz5{MkiwNVk2QTxxYU6=
Myla	NGrSZWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIPHfXoyOCEQvF2=	MmDjO\|IhcA>?	NYnGVY9wTE2VTx?=	MWrkc4V{KG6xdDDpcoR2[2ViYYDvdJRwe2m\|	MmLaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MEG5OVkoRjJ{OECxPVU6RC:jPh?=
SR786	M3\V[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MoK3NVAh\|ryP	MWC3NkBp	MnTOSG1UVw>?	MmrJ[I9meyCwb4SgbY5lfWOnIHHwc5B1d3Orcx?=	M{XpV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OECxPVU6Lz5{MkiwNVk2QTxxYU6=
HuT78	Mm\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NF\qSJoyOCEQvF2=	MXK3NkBp	MYjEUXNQ	NGnBTFhld2W\|IH7veEBqdmS3Y3WgZZBweHSxc3nz	NXTT[4d[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NFE6PTlpPkKyPFAyQTV7PD;hQi=>
MJ	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmrCNVAh\|ryP	NX;iPG1pPzJiaB?=	M2TPcmROW09?	MlS4[I9meyCwb4SgbY5lfWOnIHHwc5B1d3Orcx?=	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhyMUm1PUc,OjJ6MEG5OVk9N2F-
DERL7	NYm3dZBDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NF7z[JcyOCEQvF2=	Mn;wO\|IhcA>?	MmniSG1UVw>?	NXi1UIh4\G:nczDuc5QhcW6mdXPlJIFxd3C2b4Ppdy=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhyMUm1PUc,OjJ6MEG5OVk9N2F-
L1236	NE\1N4VHfW6ldHnvckBCe3OjeR?=	NUfPO2c2OTBizszN	M3nhZlIhcA>?		M1XIemlvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbh?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJzMEi3O{c,OjJ{MUC4O\|c9N2F-
L428	MYfGeY5kfGmxbjDBd5NigQ>?	MoLxNVAh\|ryP	MX6yJIg>		NWT1[4g5UW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;u	NEjpOYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKxNFg4Pyd-MkKyNVA5Pzd:L3G+
L591	NWf6em06TnWwY4Tpc44hSXO\|YYm=	NWPlXXZoOTBizszN	NVr5cXpWOiCq		MlT6TY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:w	MlrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MUC4O\|coRjJ{MkGwPFc4RC:jPh?=
KMH-2	NXTwRlc{TnWwY4Tpc44hSXO\|YYm=	NXfjNY5rOTBizszN	NUjaRZNROiCq		NYOzfmY4UW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;u	M3TKe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkGwPFc4Lz5{MkKxNFg4PzxxYU6=
L1236	NWGyNW9OTnWwY4Tpc44hSXO\|YYm=	M2fXe\|Uh\|ryP	M4fzR\|I1KGh?		NF\WfY5DdG:la4Ogd4VkemW2aX;uJI9nKHSqZTDDR2w2	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJzMEi3O{c,OjJ{MUC4O\|c9N2F-
L591	M3\tT2Z2dmO2aX;uJGF{e2G7	M2\rUlUh\|ryP	NWf2eXpMOjRiaB?=		NEj2WXRDdG:la4Ogd4VkemW2aX;uJI9nKHSqZTDDR2w2	NG\reWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKxNFg4Pyd-MkKyNVA5Pzd:L3G+
L1236	NWDRUHpOSXCxcITvd4l{KEG\|c3H5	MVe1JO69VQ>?	NIDQNoUzPCCq		NWjNSFdjUW6mdXP0bY9vKG:oIHHwc5B1d3Orcx?=	M1;wPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkGwPFc4Lz5{MkKxNFg4PzxxYU6=
L591	MXHBdI9xfG:\|aYOgRZN{[Xl?	MYO1JO69VQ>?	NWPycGVwOjRiaB?=		M2f1VGlv\HWldHnvckBw\iCjcH;weI9{cXN?	NV;TZ3BVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNVA5PzdpPkKyNlExQDd5PD;hQi=>
U-87MG	Mnn3SpVv[3Srb36gRZN{[Xl?	NVXSXlhmOTByIH7N	NIH2O5EzPCCq	MV\EUXNQ	MojZTY5pcWKrdHnvckBw\iBiY3XscEBucWe{YYTpc44>	NG\C[IQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PVYxQSd-MkKwO\|k3ODl:L3G+
SW1783	MX;GeY5kfGmxbjDBd5NigQ>?	NXyxO\|d1OTByIH7N	MWCyOEBp	MV;EUXNQ	M4q4bGlvcGmkaYTpc44hd2ZiIHPlcIwhdWmpcnH0bY9v	NU\NW4J1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwO\|k3ODlpPkKyNFc6PjB7PD;hQi=>
U-87MG	NXfpV3ZbTnWwY4Tpc44hSXO\|YYm=	NYPJZot[PSEQvF2=	NFKxXXAzPCCq	M3TOV2ROW09?	NELsVFlKdmirYnn0bY9vKG:oIFHreEBxcG:\|cHjvdplt[XSrb36gd5Vje3SjboTpZYxtgQ>?	NIXENnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PVYxQSd-MkKwO\|k3ODl:L3G+
SW1783	NHLwOVJHfW6ldHnvckBCe3OjeR?=	MnrpOUDPxE1?	MlvyNlQhcA>?	Ml7iSG1UVw>?	NI\QT25KdmirYnn0bY9vKG:oIFHreEBxcG:\|cHjvdplt[XSrb36gd5Vje3SjboTpZYxtgQ>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5OU[wPUc,OjJyN{m2NFk9N2F-
U-373MG	NWrKUHpPTnWwY4Tpc44hSXO\|YYm=	NYrYRlJ3PSEQvF2=	NYPPPWRxOjRiaB?=	NWXUO3NjTE2VTx?=	MkjZTY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIIP1ZpN1[W62aXHscJk>	NXvYfYs1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwO\|k3ODlpPkKyNFc6PjB7PD;hQi=>
SK-MG3	M4r4S2Z2dmO2aX;uJGF{e2G7	M1PjdVUh\|ryP	NW\SWXRqOjRiaB?=	MV;EUXNQ	M3LXWWlvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbjDzeYJ{fGGwdHnhcIx6	M4rPSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEe5OlA6Lz5{MkC3PVYxQTxxYU6=
SU-DHL-5	M1iwOGZ2dmO2aX;uJGF{e2G7	MnvxNUDPxE1?	MmXnNlQhcA>?	M1zR[mROW09?	MmL2TY5lfWO2aX;uJI9nKGGyb4D0c5Nqew>?	M4LJOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUW5OlA3Lz5{MEm1PVYxPjxxYU6=
WSU-NHL	NIThTFdHfW6ldHnvckBCe3OjeR?=	NFP3VWwyKM7:TR?=	M2\G[FI1KGh?	NGnXbYNFVVOR	M3qwSGlv\HWldHnvckBw\iCjcH;weI9{cXN?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl3OU[wOkc,OjB7NUm2NFY9N2F-
CCRF-SB	NITYOY1HfW6ldHnvckBCe3OjeR?=	NWK4cHhPOSEQvF2=	NWHwdIR4OjRiaB?=	MVrEUXNQ	Ml[0TY5lfWO2aX;uJI9nKGGyb4D0c5Nqew>?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl3OU[wOkc,OjB7NUm2NFY9N2F-
INA-6	Mo[1SpVv[3Srb36gRZN{[Xl?	NGHUN3M2KM7:TR?=	MmP4OkBp		M{LwbGlvcGmkaYTpc44hd2ZiUFmzT{9Cc3RiYX7kJGVTUyCyYYToe4F6	Mo\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3MEWxOVgoRjJyNUC1NVU5RC:jPh?=
LB	NWS5Opo3TnWwY4Tpc44hSXO\|YYm=	MXy1JO69VQ>?	NXewcldqPiCq		MVPJcohq[mm2aX;uJI9nKFCLNFuvRYt1KGGwZDDFVmsheGG2aIfhfS=>	MnHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3MEWxOVgoRjJyNUC1NVU5RC:jPh?=
B-cells	MXXGeY5kfGmxbjDhd5NigQ>?				M1q1cWlvcGmkaYTpc44hd2ZiUFmzT4RmdHSjIHnuJGIu[2WubIOgZpkheHKxbHnm[ZJifGmxbjDhd5NigSxiSVO1NEA:KDBwMEC2NUDPxE1w	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{NE[4PEc,OjJ7MkS2PFg9N2F-
MOLM14	M2POfmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MoPhN{Bl[Xm\|		MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2RTF2xOEBk\WyuczDh[pRmeiB\|IHThfZMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygTWM2OCB;IEOuOkDPxE1w	NXLhXGc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3O\|QyOjdpPkK3O\|c1OTJ5PD;hQi=>
MV4-11	MX7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MUOzJIRigXN?		NHnNNIVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{Bi\nSncjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhUUN3MDC9JFYvOyEQvF2u	Mk\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{SxNlcoRjJ5N{e0NVI4RC:jPh?=
Jurkat	MoDWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MX[zJIRigXN?		NV\TOY9NSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDKeZJs[XRiY3XscJMh[W[2ZYKgN{Bl[Xm\|IHL5JGNmdGyWaYTldk1IdG9iYYPzZZktKEmFNUCgQUA4NjlizszNMi=>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd5NEGyO{c,Ojd5N{SxNlc9N2F-
Loucy	NG\PdmNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MYOzJIRigXN?		MmHIRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCOb4XjfUBk\WyuczDh[pRmeiB\|IHThfZMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygTWM2OCB;IEiuOEDPxE1w	M3HNT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e0NVI4Lz5{N{e3OFEzPzxxYU6=
MOLT4	M1XDR2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		M1PBSVMh\GG7cx?=		NFrzVJdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3PUHQ1KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDD[YxtXGm2ZYKtS4xwKGG\|c3H5MEBKSzVyIE2gNVAvPiEQvF2u	M4XLPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e0NVI4Lz5{N{e3OFEzPzxxYU6=
insect cells	NX6wdIdPTnWwY4Tpc44h[XO\|YYm=				MlnSTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCodXzsJIxmdme2aDDIbZMufGGpZ3XkJHBKO0upYX3tZUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzMEBKSzVyIE2gNE4xQDlizszNMi=>	MnvBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd6NE[0OVEoRjJ5OES2OFUyRC:jPh?=
SUDHL6	Mn3xRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		Mn;PO\|IhcHK\|		MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOXRFjMOkBk\WyuczDt[YF{fXKnZDDh[pRmeiB5MjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVAhRSByLkGxO\|Yh\|ryPLh?=	NW\CWY9MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke4OFY1PTFpPkK3PFQ3PDVzPD;hQi=>
insect cells	MYHGeY5kfGmxbjDhd5NigQ>?				NEHpU\|lKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIH\1cIwhdGWwZ4ToJGhqey22YXfn[YQhWEl\|S3LleIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{whUUN3MDC9JFAvPTZ3IN88UU4>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzh2NkS1NUc,Ojd6NE[0OVE9N2F-
SU-DHL4	MmLKRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MmrEO\|IhcHK\|		M4P6ZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2WtSGhNPCClZXzsd{Bu\WG\|dYLl[EBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGlEPTBiPTCxMlYh\|ryPLh?=	Mor3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd6NE[0OVEoRjJ5OES2OFUyRC:jPh?=
Pfeiffer	Ml3tRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NEHTWJg4OiCqcoO=		MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCoZXnm[oVzKGOnbHzzJI1m[XO3cnXkJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OCB;IE[uPEDPxE1w	NYTSSIttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke4OFY1PTFpPkK3PFQ3PDVzPD;hQi=>
KARPAS422	NUXnVodQSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MYW3NkBpenN?		NXPtVGk5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLRXJRSVN2MkKgZ4VtdHNibXXhd5Vz\WRiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwJF0hQC5zIN88UU4>	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzh2NkS1NUc,Ojd6NE[0OVE9N2F-
Sf21	NV;t[WxjTnWwY4Tpc44h[XO\|YYm=		MUizNEBucW6\|		NXm2XFFyUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGhqezZvdHHn[4VlKHKnY3;tZolv[W62IH\1cIwudGWwZ4ToJIh2dWGwIGDJN2sheDFzMHLleIEwfW62YXfn[YQhemWlb33ibY5idnRiZoXscEBt\W6pdHigbJVu[W5icEi1ZYxxcGFiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKFOoMkGgbY5{\WO2IHPlcIx{KHW\|aX7nJHBKWDJxQWTQJIF{KHO3YoP0doF1\SCjZoTldkA{OCCvaX7zJIJ6KFSULV\SSXQh[XO\|YYmsJGlEPTBiPTCzMlch\|ryPLh?=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDFyNkm5NUc,OjhzME[5PVE9N2F-
Sf21	NHrNUllHfW6ldHnvckBie3OjeR?=		MUezNEBucW6\|		M3LJZmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDIbZM3NXSjZ3fl[EBz\WOxbXLpcoFvfCCodXzsMYxmdme2aDDoeY1idiCSSUPLJJAyOTCkZYThM5VvfGGpZ3XkJJJm[2:vYnnuZY51KG[3bHygcIVv\3SqIHj1cYFvKHB6NXHsdIhiKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBU\jJzIHnud4VkfCClZXzsd{B2e2mwZzDQTXAzN0GWUDDhd{B{fWK\|dILheIUh[W[2ZYKgN\|AhdWmwczDifUBVWi2IUlXUJIF{e2G7LDDJR\|UxKD1iMz63JO69VS5?	M4HqbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUC2PVkyLz5{OEGwOlk6OTxxYU6=
RPMI8266	MlzCRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M4PwNFczKGi{cx?=		NH\MZmFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLQUWk5OjZ4IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjByNUS5JO69VS5?	NYLROW8{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizNlU3ODFpPkK4N\|I2PjBzPD;hQi=>
Raji	NYfx[W8ySW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M{TpNFczKGi{cx?=		NYPLe4M5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCwMlAxQTl3IN88UU4>	M33zSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{K1OlAyLz5{OEOyOVYxOTxxYU6=
KARPAS422	MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?				M{j3RWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGtCWlCDU{SyNkBk\WyuczDifUBES0t6IHHzd4F6NCCJSUWwJF0hOC54ODFOwG0v	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh\|NUiwOUc,Ojh6M{W4NFU9N2F-
Pfeiffer	MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?				M1LBPWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHBn\WmoZnXyJINmdGy\|IHL5JGNEUzhiYYPzZZktKEeLNUCgQUAxNjd2IN88UU4>	NFPEeHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEizOVgxPSd-Mki4N\|U5ODV:L3G+
Saos-2	NG\G[pNyUFSVIHHzd4F6				NVLtW4NweUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=>	NHvy[Go9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-SH	MYfxTHRUKGG\|c3H5				NEPVdoRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=>	NYHtemNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R)	NVvibYtMeUiWUzDhd5NigQ>?				MUjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
NB1643	M{LPT5FJXFNiYYPzZZk>				M4T6T5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO=	NFLyZmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
OHS-50	MUHxTHRUKGG\|c3H5				M{fWW5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO=	Mlj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh41	NV3Qc25NeUiWUzDhd5NigQ>?				MnHWdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqNEGgZ4VtdHN?	NUD2W5FtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC	MXnxTHRUKGG\|c3H5				MlLCdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO=	M1n2[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1	MoCydWhVWyCjc4PhfS=>				MYjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?=	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LAN-5	MlntdWhVWyCjc4PhfS=>				NFHxXoRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO=	NHnrdW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SUDHL6	NH\oW2RCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NYnFbXdTPzJiaILz		Mlf2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVVVTIUFYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPDT\|gh[XO\|YYmsJGlEPTBiPTCwMlY2KM7:TT6=	NIq1VXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWzOFk{Pid-Mkm1N\|Q6OzZ:L3G+
RPMI8226	MWjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NGrRfpo4OiCqcoO=		M{LMZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUmDNTVgzOjZiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFUvPDlizszNMi=>	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV\|NEmzOkc,Ojl3M{S5N\|Y9N2F-
Raji	NIrXfodCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3P4PFczKGi{cx?=		NHLYfJFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLhbokh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEmuPVUh\|ryPLh?=	M1Xle\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUO0PVM3Lz5{OUWzOFk{PjxxYU6=
SU-DHL6	M2LBXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7				NYTaSWsyT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW1VvRFjMOkBk\WyuczDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5yNEKg{txONg>?	M3;RTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkCxPVkyLz5{OU[wNVk6OTxxYU6=
MOLM13	MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MWi3NkBpenN?		M3zyT2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1QVE1zMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW\|Y3XueEBie3OjeTygS2k2OCB;IEGuO{DPxE1w	NIjC[pQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEC1N\|czOSd-M{CwOVM4OjF:L3G+
MOLM14	NEjIfodIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NWnEflVQPzJiaILz		MlPrS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUW9NVTF2IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDHTVUxKD1iNj60JO69VS5?	MkKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNUO3NlEoRjNyMEWzO\|IyRC:jPh?=
MOLM14	M{TXXWFvfGm2dX3vdkBie3OjeR?=	MWixNFAhdWdxa3e=	NXH5OVlsOTRiZHH5dy=>		MlXsRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE1KGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71M452KG2xdYPlJIF{e2W\|c3XkJIF{KHS3bX;yJIdzd3e2aDDpcohq[mm2aX;uJIF1KDFyMDDt[{9s\yxicH:gZYRucW6rc4TldoVlKGSjaXz5JJZq[SCpYY\h[4Uh\G:\|ZXSg[o9zKDF2IHThfZMh[W6mIH3lZZN2emWmIHThbYx6KGS3cnnu[{Bkd22yb4Xu[EBld3OrbnegdoVt[XSrdnWgeI8h[2:wdILvcC=>	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{eyNUc,OzByNUO3NlE9N2F-
MOLM14	MoDYRY51cXS3bX;yJIF{e2G7	MX[xNFAhdWdxa3e=	NVrQXXN1OTRiZHH5dy=>		M4\sWWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3PUG0yPCClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDueU9vfSCvb4Xz[UBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZZBweHSxc3nzJIF1KDFyMDDt[{9s\yxicH:gZYRucW6rc4TldoVlKGSjaXz5JJZq[SCpYY\h[4Uh\G:\|ZXSg[o9zKDF2IHThfZMh[W6mIH3lZZN2emWmIHThbYx6KGS3cnnu[{Bkd22yb4Xu[EBld3OrbnegZpkhXFWQRVygZoF{\WRiYYPzZZk>	NEiyWVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEC1N\|czOSd-M{CwOVM4OjF:L3G+
MOLM14	M4rzd2FvfGm2dX3vdkBie3OjeR?=	MYKxNFAhdWdxa3e=	NYGwT\|JlOTRiZHH5dy=>		MkPURY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE1KGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71M452KG2xdYPlJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geJVud3JicILvcIln\XKjdHnvckAyODBibXevb4ctKHCxIHHkcYlvcXO2ZYLl[EBl[WmueTD2bYEh\2G4YXflJIRwe2WmIH\vdkAyPCCmYYnzJIFv\CCvZXHzeZJm\CCmYXnsfUBlfXKrbnegZ49ueG:3bnSg[I9{cW6pIHL5JGtqNTZ5IHzhZoVtdGmwZzDiZZNm\CCrbX31co9p	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{eyNUc,OzByNUO3NlE9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Akt(T308) / PDK1(S241) / GSK-3β(S9); PubMed: 27342398 Clin Cancer Res JeKo-1, Mino, and Granta 519 cells or cells from four different MCL patients were serum-starved for 1h and then treated with DMSO, or with 0.5, 1, or 3μM for 1h; the cells were then co-cultured with IgM (10ng/μL) for 15min before harvesting. Cell extracts were prepared, and 30μg (cell lines) or 50μg (primary cells) protein was loaded for immunoblot analyses. The effects of idelalisib on Akt (Thr308) and total Akt, PDK1 (Ser241) and total PDK1, GS3K-3β (Ser9) and total GSK-3β protein expression levels were detected in (A) JeKo-1, Mino and Granta 519 cells. p-FoxO3a / FoxO3a; PubMed: 30224718 Cell Death Dis HepG2 cells were treated with 5 μmol/L idelalisib for 24 h. Indicated protein expression was analyzed by western blotting and p-FoxO3a normalized to FoxO3a, p-AKT normalized to AKT. The data represent the mean ± SD of three independent experiments. P < 0.01 (one-way ANOVA with Tukey’s post hoc test). Mcl-1 / Bcl-2 / Bid / Bcl-xl / Noxa / Bak / Bax ; PubMed: 30224718 Cell Death Dis HepG2 cells were treated with 5 μmol/L idelalisib at indicated time points. The expression of indicated Bcl-2 family members was analyzed by western blotting and normalized to β-actin. The data represent the mean ± SD of three independent experiments. P < 0.01 (one-way ANOVA with Tukey’s post hoc test). Cleaved caspase 3 / Cleaved caspase 9; PubMed: 28008149 Oncotarget HepG2 cells were treated with 5μmol/L idelalisib at indicated time point. Cleaved-caspase 3 and 9 were analyzed by western blotting. p-AKT / AKT; PubMed: 28008149 Oncotarget HCT116 cells were treated with 10 μmol/L idelalisib at indicated time point. Total AKT and p-AKT expression was analyzed Western blotting. p-p65; PubMed: 28008149 Oncotarget HCT116 cells were treated with 10 μmol/L idelalisib at indicated time point. p-p65 (S536) and p65 expression was analyzed by Western blotting. Bim / Bcl-xl / Bid / Mcl-1; PubMed: 28008149 Oncotarget HCT116 cells treated with 10 μmol/L idelalisib at indicated time point. The expression of indicated Bcl-2 family members was analyzed by Western blotting. PUMA / p53 ; PubMed: 28008149 Oncotarget Parental and p53-KD HCT116 cells were treated with 10 μmol/L idelalisib for 24 hours. PUMA expression was analyzed by Western blotting.	27342398 30224718 28008149
Growth inhibition assay	Cell viability; PubMed: 30224718 Cell Death Dis The indicated cell lines were treated with increasing concentrations of idelalisib for 72 h. Cell viability was determined by MTS assay. Cell viability; PubMed: 28008149 Oncotarget Indicated cell lines were treated with different concentrations of idelalisib for 72 hours. Cell proliferation was determined by MTS assay. Results were expressed as means ± SD of three independent experiments.	30224718 28008149

激酶实验：^[2]	- 合并 PI3K实验: 用全CLL和正常B细胞溶解物进行PI3K实验。进行PI3K ELISA 实验。全细胞抽提物加到PI(4,5)P2 底物和反应 buffer（包含 ATP)的混合物中，在室温下温育。加入 PI(3,4,5)P3 探测器和 EDTA混合，反应终止，在室温下温育1小时。混合物转移到PI3K ELISA板上，再温育1小时。冲洗反应板，然后和第二探测器再温育30分钟。再次冲洗反应板, 加入3,3′,5,5′-四甲基联苯胺溶液，反应5分钟，加入H₂SO₄ 终止反应。在450纳米处读数。
细胞实验：^[2]	- 合并 Cell lines: CLL B 细胞或健康志愿者的T细胞或NK细胞 Concentrations: 0.01-100 μM Incubation Time: 48小时 Method: 进行MTT实验测定细胞毒性。1×10⁵个细胞和CAL-101一起温育。加入MTT试剂, 再次温育20小时，然后用溶于PBS的硫酸鱼精蛋白冲洗。加入DMSO, 用分光光度计在540纳米处测定吸光度。使用膜联蛋白/PI液式细胞计检查在不同时间点测定细胞存活力，分析数据。每个样本至少计数10⁴个细胞。以全部阳性细胞与未处理细胞之比百分数的形式来表示实验结果。加入100 μM Z-VAD检测caspase-依赖的细胞凋亡。加入 1 μg/mL CD40L, 800 U/mL IL-4, 50 ng/mL BAFF, 20 ng/mL TNF-α测定存活信号, 或者共培养在纤连蛋白或 HS-5 细胞系基质包被的板上。基质共培养在75cm²培养瓶 (80%-100% 融合率) 培养24 小时，然后加入CLL细胞。 (Only for Reference)

参考文献

溶解度 (25°C)

体外	DMSO	83 mg/mL warmed (199.79 mM)
	Ethanol	23 mg/mL (55.36 mM)
	Water	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+20%PEG 300+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Idelalisib (CAL-101, GS-1101) SDF

分子量	415.42
化学式	C₂₂H₁₈FN₇O
CAS号	870281-82-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-05-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03582098	Completed	Drug: Idelalisib\|Drug: Rituximab	Chronic Lymphocytic Leukaemia	Gilead Sciences	September 12 2018	--
NCT03151057	Active not recruiting	Drug: Idelalisib 100 MG\|Drug: Placebo Oral Tablet	B Cells-Tumors\|B Cell Chronic Lymphocytic Leukemia\|Follicular Lymphoma\|Mantle Cell Lymphoma\|Large B-Cell Diffuse Lymphoma of Bone (Diagnosis)	Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins\|Gilead Sciences	July 31 2018	Phase 1
NCT03568929	Recruiting	Drug: Idelalisib	Follicular Non-Hodgkin''s Lymphoma Refractory	Gilead Sciences	May 25 2018	--

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
What is the recommended dose of CAL-101 and the route of administration for mouse studies?
回答：
According to the following paper, S2226 can be used by I.V. administration at the concentration of 40 mg/kg. https://www.ncbi.nlm.nih.gov/pubmed/24625684

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

泛PI3K抑制剂

LY294002 : Pan-PI3Kα/δ/β inhibitor, IC50=0.5 μM/0.57 μM/0.97 μM.
活性最高的PI3K抑制剂

Duvelisib (IPI-145, INK1197) : PI3K δ, IC50=1 nM.
用于临床的PI3K抑制剂

Hexestrol : Phase II for Advanced Breast Cancer.
最新的PI3K抑制剂

GSK2636771 : Potent, orally bioavailable, PI3Kβ-selective inhibitor, sensitive to PTEN null cell lines.

研究领域

产品类型

定制您的化合物库

Idelalisib (CAL-101, GS-1101)

客户使用Selleck生产的Idelalisib (CAL-101, GS-1101)发表文献201篇：

产品安全说明书

PI3K抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Idelalisib (CAL-101, GS-1101) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-05-17)

技术支持

常见问题及建议解决方法

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

相关PI3K产品