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Idelalisib

目录号：S2226 别名: CAL-101, GS-1101 中文名称：艾代拉里斯，艾代拉利司

仅限科研使用

Idelalisib (CAL-101, GS-1101) 是选择性p110δ抑制剂，在无细胞试验中IC50为 2.5 nM；对 p110δ 表现出的选择性是对 p110α/β/γ 的 40 到 300 倍，对p110δ的选择性是对 C2β，hVPS34，DNA-PK 和 mTOR的400到4000倍。Idelalisib 还可诱导自噬。

CAS: 870281-82-6

规格	价格	库存
10mM (1mL in DMSO)	RMB 1279.19	现货
10mg	RMB 972.85	现货
50mg	RMB 3867.24	现货
200mg	RMB 10401.3	现货
大包装	有超大折扣

今日订购，明日送达全国免运费分装免费分装成小规格产品（单支最小为1mg）如1支10mg产品可分装为10支1mg产品全国免费电话：400-668-6834 \| Email：info@selleck.cn

客户使用Selleck生产的Idelalisib 发表文献233篇：

产品安全说明书

PI3K抑制剂选择性比较

生物活性

产品描述

特性

Calistoga 暗示 CAL-101治疗血液恶性肿瘤可能有更广泛的应用价值。

靶点

p110δ ^[1] (Cell-free assay)	p110γ ^[1] (Cell-free assay)
2.5 nM	89 nM

体外研究

CAL-101 对p110α, p110β,和p110γ作用效果不大。CAL-101作用于原代嗜碱细胞特定阻断FcϵR1 p110δ调节的 CD63表达，EC50 为8 nM。与急性髓性白血病(AML) 和骨髓增生性肿瘤(MPN) 细胞相比，CAL-101 作用于B-cell急性淋巴细胞白血病(B-ALL)和慢性淋巴细胞白血病(CLL) 细胞时显示更强的活性。CAL-101 作用于SU-DHL-5, KARPAS-422 和CCRF-SB细胞，降低pAktS473, pAktT308, 和下游靶点S6， EC50为0.1到1.0 μM。 ^[1] CAL-101 作用于CLL细胞，诱导选择性细胞毒性，不是通过突变状态或间期细胞遗传学,主要通过caspase依赖机制。与正常B细胞相比，CAL-101作用于CLL 细胞优先产生细胞毒性，和LY294002相比，作用于其他造血细胞不会产生毒性。CAL-101 作用于T 细胞和天然杀伤细胞缺乏直接的细胞毒性潜能。CAL-101抑制炎症细胞因子的产生,比如 IL-6, IL-10, TNF-α,和IFN-γ,且激活诱导的细胞因子,如CD40L。CAL-101 也抗CD40L调节的CLL细胞存活。^[2] CAL-101 作用于L1236和L591细胞, 诱导细胞在G1期积累，在S期下降，说明 CAL-101可以作为治疗霍杰金淋巴瘤(HL)的一种新策略。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

MEC1	MlXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NUnwbmxxTE2VTx?=	MkTjTWM2OD1{MD60JO69VQ>?	Ml6yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OUmzOVIoRjJ3OUm5N\|UzRC:jPh?=
CLL PBMCs	Ml6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M2PFN2ROW09?	M2HhTWlEPTB;Mj65JI5O	MmjvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7MUeyOlcoRjJ3OUG3NlY4RC:jPh?=
U266	NEDV[VBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MYm0NEDPxE1?	NEjXUYE1QCCq		Mn\UO\|kvPSViaX7obYJqfGmxbjDyZZRm	NX3VdGFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN\|k{OzJpPkK1N\|M6OzN{PD;hQi=>
K562	MXHGeY5kfGmxbjDBd5NigQ>?	MoC2NUDPxE1?	MWSzJIg>		MoLqTY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:w	NYDtTZJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVQ4PzVpPkK1NFE1Pzd3PD;hQi=>
K562	M2DNPWZ2dmO2aX;uJGF{e2G7	MV:xJO69VQ>?	NGrsO4E{KGh?		NIrZN5pKdmirYnn0bY9vKG:oIGC3NHM3UyCyaH;zdIhwenmuYYTpc44>	NFPxXm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxOFc4PSd-MkWwNVQ4PzV:L3G+
K562	NWH3flJETnWwY4Tpc44hSXO\|YYm=	MUixJO69VQ>?	MmezN{Bp		NIm1bGJKdmirYnn0bY9vKG:oIFfTT\|MheGixc4Doc5J6dGG2aX;u	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzNEe3OUc,OjVyMUS3O\|U9N2F-
K562	NVLaPYs3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWexJO69VQ>?	MUG3NkBp		NWXzUoNSUW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9v	NF;m[YI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxOFc4PSd-MkWwNVQ4PzV:L3G+
Primary AML cell	MkO3SpVv[3Srb36gRZN{[Xl?	NVvsRXZvOSEQvF2=	M1PkSFMhcA>?		M2LLVmlvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbh?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzNEe3OUc,OjVyMUS3O\|U9N2F-
Primary AML cell	M4T4TmZ2dmO2aX;uJGF{e2G7	NV;w[ZZSOSEQvF2=	MYGzJIg>		NEfMUoVKdmirYnn0bY9vKG:oIGC3NHM3UyCyaH;zdIhwenmuYYTpc44>	Mkf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUS3O\|UoRjJ3MEG0O\|c2RC:jPh?=
Primary AML cell	M3jTdmZ2dmO2aX;uJGF{e2G7	NEjCV5IyKM7:TR?=	MlK0N{Bp		NI\OTYhKdmirYnn0bY9vKG:oIFfTT\|MheGixc4Doc5J6dGG2aX;u	MmrZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMUS3O\|UoRjJ3MEG0O\|c2RC:jPh?=
Primary AML cell	NF7GVWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NGqwbW8yKM7:TR?=	MnW5N{Bp		MUDTeZBxemW\|c3nvckBw\iC{Ul7BJJN6dnSqZYPpdy=>	NIDYcJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxOFc4PSd-MkWwNVQ4PzV:L3G+
Microglia	M4nLcmZ2dmO2aX;uJGF{e2G7	MYO1JO69VQ>?	MY[xNEBp	NVn2UIlkTE2VTx?=	NHz5PXBF\WO{ZXHz[UBw\iCWTl\hJJNm[3KndHnvckBnem:vIFzQV{1{fGmvdXzheIVlKCCyMUGw{tRFQTFyQT;EPVExSSCvaXPyc4dtcWF?	M3jJPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK1Olg1Lz5{NE[yOVY5PDxxYU6=
Primary CLL cell	MlTmSpVv[3Srb36gRZN{[Xl?	M4LiRVEh\|ryP	MYexOUBucW5?	MUjEUXNQ	MnHVRoxw[2u\|IFLDVk1qdmS3Y3XkJGxEWDFic3XybY5mNTViYXP0bZZifGmxbh?=	NIDscZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECwPVI{Oyd-MkSwNFkzOzN:L3G+
JEKO-1	MmGzSpVv[3Srb36gRZN{[Xl?	MWixJO69VQ>?	Mlr1O\|IhcA>?		Mlz0TY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIHnuJGloVS2\|dHnteYxifGWmIFrFT28uOQ>?	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2MUW0NUc,OjN\|NEG1OFE9N2F-
Granta-519	NX\TepZ1TnWwY4Tpc44hSXO\|YYm=	MVixJO69VQ>?	MUWyJIg>		NE\QZVVKdmirYnn0bY9vKG:oIFHreEh1OzB6KTDwbI9{eGixconsZZRqd25?	Ml;iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NEG1OFEoRjJ\|M{SxOVQyRC:jPh?=
Granta-519	MV3GeY5kfGmxbjDBd5NigQ>?	NXnyN2d{OSEQvF2=	NYrYZoJiOiCq		MYXJcohq[mm2aX;uJI9nKEGtdDjzOFc{MSCyaH;zdIhwenmuYYTpc44>	M1TtelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{SxOVQyLz5{M{O0NVU1OTxxYU6=
JEKO-1	NGfXeXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVjMb412OTBizszN	MV[3NkBp		MXzJcohq[mm2aX;uJI9nKHC{b3zp[oVz[XSrb36gd4xq\2i2bIm=	NH3zSng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O0NVU1OSd-MkOzOFE2PDF:L3G+
JEKO-1	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1;6dFUh\|ryP	M1LkZ\|czKGh?		MnLQ[I9meyCwb4SgbY5lfWOnIHPlcIwh[3mlbHWgZZJz\XO2IH;yJIFxd3C2b4Ppdy=>	MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{[yNlAoRjJ\|Nke2NlIxRC:jPh?=
MAVER-1	M4HESGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHnkRoo2KM7:TR?=	NWDYVZpqPzJiaB?=		NE\H[VZld2W\|IH7veEBqdmS3Y3WgZ4VtdCCleXPs[UBienKnc4Sgc5Ih[XCxcITvd4l{	MoPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{[yNlAoRjJ\|Nke2NlIxRC:jPh?=
MINO	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MW[1JO69VQ>?	NHXaOnc4OiCq		MVPkc4V{KG6xdDDpcoR2[2ViY3XscEBkgWOuZTDhdpJme3Rib4KgZZBweHSxc3nz	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ5NkKyNEc,OjN4N{[yNlA9N2F-
SP53	MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3;Wd\|AvOSEQvF2=	M1v0[FczKGh?		NGrUUIpld2W\|IH7veEBqdmS3Y3WgZ4VtdCCleXPs[UBienKnc4Sgc5Ih[XCxcITvd4l{	M3O5UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Nke2NlIxLz5{M{[3OlIzODxxYU6=
HH	M3rHR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mm[4NVAh\|ryP	M2n0NlczKGh?	MWrEUXNQ	M4XNW2lv\HWldHnvckBw\iCjcH;weI9{cXNic3zp[4h1dHl?	NYLZcYJjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NFE6PTlpPkKyPFAyQTV7PD;hQi=>
Myla	NULxTpFlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFXFdoMyOCEQvF2=	MWO3NkBp	M17WXGROW09?	NH[wWpFld2W\|IH7veEBqdmS3Y3WgZZBweHSxc3nz	NVvvRlgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NFE6PTlpPkKyPFAyQTV7PD;hQi=>
SR786	M4nPc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEfreYkyOCEQvF2=	M4\QSFczKGh?	NUjwcpZVTE2VTx?=	MojU[I9meyCwb4SgbY5lfWOnIHHwc5B1d3Orcx?=	M3\JU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OECxPVU6Lz5{MkiwNVk2QTxxYU6=
HuT78	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVWxNEDPxE1?	NV6wPHJ[PzJiaB?=	MUPEUXNQ	MmO5[I9meyCwb4SgbY5lfWOnIHHwc5B1d3Orcx?=	NXG4NHJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NFE6PTlpPkKyPFAyQTV7PD;hQi=>
MJ	NGq3SI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFXwUHQyOCEQvF2=	NF3OSYM4OiCq	NX;RN4JSTE2VTx?=	NHvDO\|hld2W\|IH7veEBqdmS3Y3WgZZBweHSxc3nz	Mln5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MEG5OVkoRjJ{OECxPVU6RC:jPh?=
DERL7	NHTSSmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2LEc\|ExKM7:TR?=	NYfudY5pPzJiaB?=	NGPpV3FFVVOR	NVLyXJVp\G:nczDuc5QhcW6mdXPlJIFxd3C2b4Ppdy=>	NWq2T2l4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NFE6PTlpPkKyPFAyQTV7PD;hQi=>
L1236	M1ywUmZ2dmO2aX;uJGF{e2G7	MXuxNEDPxE1?	M2rqeVIhcA>?		NXLEVpN7UW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;u	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJzMEi3O{c,OjJ{MUC4O\|c9N2F-
L428	MXPGeY5kfGmxbjDBd5NigQ>?	MYWxNEDPxE1?	NHnJeJczKGh?		M1S5VGlvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbh?=	NX3xWIN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNVA5PzdpPkKyNlExQDd5PD;hQi=>
L591	NILmPXFHfW6ldHnvckBCe3OjeR?=	MkGzNVAh\|ryP	NFL4TIczKGh?		M3;heGlvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbh?=	MlPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MUC4O\|coRjJ{MkGwPFc4RC:jPh?=
KMH-2	NIH5VmZHfW6ldHnvckBCe3OjeR?=	MmnuNVAh\|ryP	NF63e\|gzKGh?		NXO3XmM1UW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;u	NGPrSVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKxNFg4Pyd-MkKyNVA5Pzd:L3G+
L1236	NVi2ToY1TnWwY4Tpc44hSXO\|YYm=	NXOzbXlNPSEQvF2=	MV:yOEBp		MmG5Roxw[2u\|IIPlZ5JmfGmxbjDv[kB1cGViQ1PMOS=>	MkfJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MUC4O\|coRjJ{MkGwPFc4RC:jPh?=
L591	M2\0O2Z2dmO2aX;uJGF{e2G7	MlLoOUDPxE1?	NWX2dZVwOjRiaB?=		NV\jXoVOSmyxY3vzJJNm[3KndHnvckBw\iC2aHWgR2NNPQ>?	MnfvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MUC4O\|coRjJ{MkGwPFc4RC:jPh?=
L1236	NYPLcZc6SXCxcITvd4l{KEG\|c3H5	M2LadFUh\|ryP	NXvReIhLOjRiaB?=		NWfNbnFrUW6mdXP0bY9vKG:oIHHwc5B1d3Orcx?=	MlLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MUC4O\|coRjJ{MkGwPFc4RC:jPh?=
L591	MlvIRZBweHSxc3nzJGF{e2G7	MoHJOUDPxE1?	NX3CVpNHOjRiaB?=		NVnmVZU3UW6mdXP0bY9vKG:oIHHwc5B1d3Orcx?=	M{LKVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkGwPFc4Lz5{MkKxNFg4PzxxYU6=
U-87MG	MmW2SpVv[3Srb36gRZN{[Xl?	MkLnNVAxKG6P	M1LMO\|I1KGh?	NY\ycIVCTE2VTx?=	MV7Jcohq[mm2aX;uJI9nKCClZXzsJI1q\3KjdHnvci=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5OU[wPUc,OjJyN{m2NFk9N2F-
SW1783	MoG3SpVv[3Srb36gRZN{[Xl?	MVyxNFAhdk1?	M1n6elI1KGh?	M3rqfmROW09?	Mn\VTY5pcWKrdHnvckBw\iBiY3XscEBucWe{YYTpc44>	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5OU[wPUc,OjJyN{m2NFk9N2F-
U-87MG	M4S0eWZ2dmO2aX;uJGF{e2G7	M1v4c\|Uh\|ryP	NGToT4gzPCCq	NGXkdJFFVVOR	M13FT2lvcGmkaYTpc44hd2ZiQXv0JJBpd3OyaH;yfYxifGmxbjDzeYJ{fGGwdHnhcIx6	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5OU[wPUc,OjJyN{m2NFk9N2F-
SW1783	Mmf6SpVv[3Srb36gRZN{[Xl?	NUGwPHJ7PSEQvF2=	MUiyOEBp	MXvEUXNQ	NGfrd4VKdmirYnn0bY9vKG:oIFHreEBxcG:\|cHjvdplt[XSrb36gd5Vje3SjboTpZYxtgQ>?	M{DW[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEe5OlA6Lz5{MkC3PVYxQTxxYU6=
U-373MG	MWLGeY5kfGmxbjDBd5NigQ>?	NV7jTox{PSEQvF2=	NWn0WI4xOjRiaB?=	NH7mNnhFVVOR	NV\sb5FwUW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;uJJN2[nO2YX70bYFtdHl?	NHvEc\|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PVYxQSd-MkKwO\|k3ODl:L3G+
SK-MG3	NULLdo14TnWwY4Tpc44hSXO\|YYm=	MkjEOUDPxE1?	M{XpOVI1KGh?	NGn0cmJFVVOR	NWHEWVREUW6qaXLpeIlwdiCxZjDBb5QheGixc4Doc5J6dGG2aX;uJJN2[nO2YX70bYFtdHl?	MnXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{m2NFkoRjJ{MEe5OlA6RC:jPh?=
SU-DHL-5	M1PXWGZ2dmO2aX;uJGF{e2G7	MorSNUDPxE1?	MnrtNlQhcA>?	Moi5SG1UVw>?	M17nbmlv\HWldHnvckBw\iCjcH;weI9{cXN?	MkLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7NUm2NFYoRjJyOUW5OlA3RC:jPh?=
WSU-NHL	M2ixcGZ2dmO2aX;uJGF{e2G7	MoDINUDPxE1?	MW[yOEBp	MlW1SG1UVw>?	NVXsfZk3UW6mdXP0bY9vKG:oIHHwc5B1d3Orcx?=	NVnKWZhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC5OVk3ODZpPkKwPVU6PjB4PD;hQi=>
CCRF-SB	M3PpUmZ2dmO2aX;uJGF{e2G7	MV6xJO69VQ>?	MX6yOEBp	M4D4cGROW09?	MoXVTY5lfWO2aX;uJI9nKGGyb4D0c5Nqew>?	MkTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7NUm2NFYoRjJyOUW5OlA3RC:jPh?=
INA-6	NGjy[5dHfW6ldHnvckBCe3OjeR?=	NWnmNXNXPSEQvF2=	NUK0RVUxPiCq		MknWTY5pcWKrdHnvckBw\iCSSUPLM2FsfCCjbnSgSXJMKHCjdHj3ZZk>	MnrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3MEWxOVgoRjJyNUC1NVU5RC:jPh?=
LB	M1LEe2Z2dmO2aX;uJGF{e2G7	M4XGTFUh\|ryP	MmXzOkBp		NVTtR3FsUW6qaXLpeIlwdiCxZjDQTVRMN0GtdDDhcoQhTVKNIIDheIh4[Xl?	NUnE[HpWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1NFUyPThpPkKwOVA2OTV6PD;hQi=>
B-cells	M1fM[GZ2dmO2aX;uJIF{e2G7				MkP1TY5pcWKrdHnvckBw\iCSSUPL[IVtfGFiaX6gRk1k\WyuczDifUBxem:uaX\ldoF1cW:wIHHzd4F6NCCLQ{WwJF0hOC5yME[xJO69VS5?	NETTOWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOFY5QCd-MkK5NlQ3QDh:L3G+
MOLM14	M4TodWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NVL3cGFNOyCmYYnz		Mkf3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zNNVQh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IFPlcIxVcXSncj3HcI8h[XO\|YYmsJGlEPTBiPTCzMlYh\|ryPLh?=	NFTrNno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{e3OFEzPyd-Mke3O\|QyOjd:L3G+
MV4-11	NI\LcFRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NVfxdVRUOyCmYYnz		MonTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPVkStNVEh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IFPlcIxVcXSncj3HcI8h[XO\|YYmsJGlEPTBiPTC2MlMh\|ryPLh?=	NYTzTZNIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3O\|QyOjdpPkK3O\|c1OTJ5PD;hQi=>
Jurkat	M{HZVWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M4f4O\|Mh\GG7cx?=		MlTGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCMdYLrZZQh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IFPlcIxVcXSncj3HcI8h[XO\|YYmsJGlEPTBiPTC3Mlkh\|ryPLh?=	MnPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{SxNlcoRjJ5N{e0NVI4RC:jPh?=
Loucy	MUXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M33QdlMh\GG7cx?=		MkP5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCOb4XjfUBk\WyuczDh[pRmeiB\|IHThfZMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygTWM2OCB;IEiuOEDPxE1w	M1KzZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e0NVI4Lz5{N{e3OFEzPzxxYU6=
MOLT4	NWHtcpV2SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M{XRU\|Mh\GG7cx?=		NXPkRpZySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bi\nSncjCzJIRigXNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhUUN3MDC9JFExNjZizszNMi=>	MmjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{SxNlcoRjJ5N{e0NVI4RC:jPh?=
insect cells	NE\FW\|BHfW6ldHnvckBie3OjeR?=				NW\ibGNpUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDmeYxtKGynbnf0bEBJcXNvdHHn[4VlKFCLM1vnZY1u[SCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{NCCLQ{WwJF0hOC5yOEmg{txONg>?	NEm4XGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{i0OlQ2OSd-Mke4OFY1PTF:L3G+
SUDHL6	NV;Lb4Q5SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M13JTlczKGi{cx?=		MlnQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVVVTIUFYh[2WubIOgcYVie3W{ZXSgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR\|UxKD1iMD6xNVc3KM7:TT6=	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzh2NkS1NUc,Ojd6NE[0OVE9N2F-
insect cells	MYrGeY5kfGmxbjDhd5NigQ>?				NWTnXmgxUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDmeYxtKGynbnf0bEBJcXNvdHHn[4VlKFCLM1vi[ZRiKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMtKEmFNUCgQUAxNjV4NTFOwG0v	NFnJfW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{i0OlQ2OSd-Mke4OFY1PTF:L3G+
SU-DHL4	MkDPRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NE\UPIg4OiCqcoO=		M{DC[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2WtSGhNPCClZXzsd{Bu\WG\|dYLl[EBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGlEPTBiPTCxMlYh\|ryPLh?=	MlnvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd6NE[0OVEoRjJ5OES2OFUyRC:jPh?=
Pfeiffer	M1;PZWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NUTQPHh1PzJiaILz		MljURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCSZnXp[oZmeiClZXzsd{Bu\WG\|dYLl[EBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGlEPTBiPTC2Mlgh\|ryPLh?=	M4LFXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OES2OFUyLz5{N{i0OlQ2OTxxYU6=
KARPAS422	MmHXRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MojQO\|IhcHK\|		NX\TflVDSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLRXJRSVN2MkKgZ4VtdHNibXXhd5Vz\WRiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwJF0hQC5zIN88UU4>	M1nGU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OES2OFUyLz5{N{i0OlQ2OTxxYU6=
Sf21	M{fTSWZ2dmO2aX;uJIF{e2G7		MkLYN\|AhdWmwcx?=		NILwUoNKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygTIl{Pi22YXfn[YQhemWlb33ibY5idnRiZoXscE1t\W6pdHigbJVu[W5iUFmzT{BxOTFyYnX0ZU92dnSjZ3fl[EBz\WOxbXLpcoFvfCCodXzsJIxmdme2aDDoeY1idiCyOEXhcJBp[SCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhW2Z{MTDpcpNm[3RiY3XscJMhfXOrbnegVGlROi:DVGCgZZMhe3Wkc4TyZZRmKGGodHXyJFMxKG2rboOgZpkhXFJvRmLFWEBie3OjeTygTWM2OCB;IEOuO{DPxE1w	NXXuSpdGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixNFY6QTFpPkK4NVA3QTlzPD;hQi=>
Sf21	NGq2VnZHfW6ldHnvckBie3OjeR?=		MUKzNEBucW6\|		NInJVlZKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygTIl{Pi22YXfn[YQhemWlb33ibY5idnRiZoXscE1t\W6pdHigbJVu[W5iUFmzT{BxOTFyYnX0ZU92dnSjZ3fl[EBz\WOxbXLpcoFvfCCodXzsJIxmdme2aDDoeY1idiCyOEXhcJBp[SCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhW2Z{MTDpcpNm[3RiY3XscJMhfXOrbnegVGlROi:DVGCgZZMhe3Wkc4TyZZRmKGGodHXyJFMxKG2rboOgZpkhXFJvRmLFWEBie3OjeTygTWM2OCB;IEOuO{DPxE1w	M4[1TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUC2PVkyLz5{OEGwOlk6OTxxYU6=
RPMI8266	M3HRNGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MVO3NkBpenN?		M3HCZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUmDNTVgzPjZiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODB3NEmg{txONg>?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN{NU[wNUc,Ojh\|MkW2NFE9N2F-
Raji	NIXoNnhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MnzSO\|IhcHK\|		MojHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCUYXrpJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkCwPVk2KM7:TT6=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN{NU[wNUc,Ojh\|MkW2NFE9N2F-
KARPAS422	NWnLN3dET3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=				MXfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDLRXJRSVN2MkKgZ4VtdHNiYomgR2NMQCCjc4PhfUwhT0l3MDC9JFAvPjhizszNMi=>	NWDZTlZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4N\|U5ODVpPkK4PFM2QDB3PD;hQi=>
Pfeiffer	NEPHU5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?=				M4TBbWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHBn\WmoZnXyJINmdGy\|IHL5JGNEUzhiYYPzZZktKEeLNUCgQUAxNjd2IN88UU4>	M4D6NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEO1PFA2Lz5{OEizOVgxPTxxYU6=
Saos-2	MnjsdWhVWyCjc4PhfS=>				Mn\NdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?=	MnTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH	MmnSdWhVWyCjc4PhfS=>				MUPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?=	NF3HNpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
MG 63 (6-TG R)	Mlu4dWhVWyCjc4PhfS=>				M2\GPZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?=	MnXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
NB1643	M17QepFJXFNiYYPzZZk>				NIfLO2RyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz	NFjsdlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
OHS-50	NWLaboJmeUiWUzDhd5NigQ>?				NXvZenhbeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF;IV{02OCClZXzsdy=>	NXn1TXI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41	MY\xTHRUKGG\|c3H5				NHTJZ5ZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SK-N-MC	NWq1Z5NpeUiWUzDhd5NigQ>?				NVLsZYdXeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM>	M3f2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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LAN-5	NGrCNXlyUFSVIHHzd4F6				Mn3ydWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy\|	NIPWd5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SUDHL6	MnKxRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NF3Me444OiCqcoO=		MoPpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVVVTIUFYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPDT\|gh[XO\|YYmsJGlEPTBiPTCwMlY2KM7:TT6=	NG\5do49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWzOFk{Pid-Mkm1N\|Q6OzZ:L3G+
RPMI8226	NYfmTmpPSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M4fZPVczKGi{cx?=		MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKSTVm4NlI3KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTC1MlQ6KM7:TT6=	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV\|NEmzOkc,Ojl3M{S5N\|Y9N2F-
Raji	MmnzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MXu3NkBpenN?		M2q4SmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUnHqbUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iOT65OUDPxE1w	NEnhbXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWzOFk{Pid-Mkm1N\|Q6OzZ:L3G+
SU-DHL6	M13zO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7				MVnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTWU1FUEx4IHPlcIx{KGK7IFPlcIxVcXSncj3HcI8h[XO\|YYmsJGdKPTBiPTCwMlA1OiEQvF2u	M{XxfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkCxPVkyLz5{OU[wNVk6OTxxYU6=
MOLM13	MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M4nyV\|czKGi{cx?=		NVjJTGV7T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OTUGzJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIx2dWmwZYPj[Y51KGG\|c3H5MEBIUTVyIE2gNU44KM7:TT6=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{eyNUc,OzByNUO3NlE9N2F-
MOLM14	NEL2c\|ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NHPPdo44OiCqcoO=		MkXYS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUW9NVTF2IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDHTVUxKD1iNj60JO69VS5?	MlnMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNUO3NlEoRjNyMEWzO\|IyRC:jPh?=
MOLM14	MVTBcpRqfHWvb4KgZZN{[Xl?	MlP4NVAxKG2pL3vn	M2T2VVE1KGSjeYO=		MUnBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zNNVQh[2WubIOgfIVvd2e{YX\0[YQhcW5iboWvcpUhdW:3c3WgZZN{\XO\|ZXSgZZMhfHWvb4Kg[5Jwf3SqIHnubIljcXSrb36gZZQhOTByIH3nM4toNCCybzDh[I1qdmm\|dHXy[YQh\GGrbImgeoliKGejdnHn[UBld3OnZDDmc5IhOTRiZHH5d{BidmRibXXhd5Vz\WRiZHHpcJkh\HW{aX7nJINwdXCxdX7kJIRwe2mwZzDy[YxifGm4ZTD0c{Bkd262cn;s	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB3M{eyNUc,OzByNUO3NlE9N2F-
MOLM14	MWTBcpRqfHWvb4KgZZN{[Xl?	Mnn6NVAxKG2pL3vn	MXyxOEBl[Xm\|		MVHBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zNNVQh[2WubIOgfIVvd2e{YX\0[YQhcW5iboWvcpUhdW:3c3WgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIHHwc5B1d3OrczDheEAyODBibXevb4ctKHCxIHHkcYlvcXO2ZYLl[EBl[WmueTD2bYEh\2G4YXflJIRwe2WmIH\vdkAyPCCmYYnzJIFv\CCvZXHzeZJm\CCmYXnsfUBlfXKrbnegZ49ueG:3bnSg[I9{cW6pIHL5JHRWVkWOIHLhd4VlKGG\|c3H5	M3LXU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEWzO\|IyLz5\|MEC1N\|czOTxxYU6=
MOLM14	M1;HVGFvfGm2dX3vdkBie3OjeR?=	MV:xNFAhdWdxa3e=	MW[xOEBl[Xm\|		MULBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zNNVQh[2WubIOgfIVvd2e{YX\0[YQhcW5iboWvcpUhdW:3c3WgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0eY1weiCycn;sbYZmemG2aX;uJFExOCCvZz;r[{wheG9iYXTtbY5qe3SncnXkJIRicWy7II\pZUBo[X[jZ3Wg[I9{\WRiZn;yJFE1KGSjeYOgZY5lKG2nYYP1doVlKGSjaXz5JIR2emmwZzDjc41xd3WwZDDkc5NqdmdiYomgT4kuPjdibHHi[YxtcW6pIHLhd4VlKGmvbYXuc4g>	NGfTeIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEC1N\|czOSd-M{CwOVM4OjF:L3G+

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Akt(T308) / PDK1(S241) / GSK-3β(S9) p-FoxO3a / FoxO3a Mcl-1 / Bcl-2 / Bid / Bcl-xl / Noxa / Bak / Bax Cleaved caspase 3 / Cleaved caspase 9 p-AKT / AKT p-p65 Bim / Bcl-xl / Bid / Mcl-1 PUMA / p53	27342398 30224718 28008149
Growth inhibition assay	Cell viability Cell viability	30224718 28008149

激酶实验：^[2]	PI3K实验: 用全CLL和正常B细胞溶解物进行PI3K实验。进行PI3K ELISA 实验。全细胞抽提物加到PI(4,5)P2 底物和反应 buffer（包含 ATP)的混合物中，在室温下温育。加入 PI(3,4,5)P3 探测器和 EDTA混合，反应终止，在室温下温育1小时。混合物转移到PI3K ELISA板上，再温育1小时。冲洗反应板，然后和第二探测器再温育30分钟。再次冲洗反应板, 加入3,3′,5,5′-四甲基联苯胺溶液，反应5分钟，加入H₂SO₄ 终止反应。在450纳米处读数。
细胞实验：^[2]	Cell lines: CLL B 细胞或健康志愿者的T细胞或NK细胞 Concentrations: 0.01-100 μM Incubation Time: 48小时 Method: 进行MTT实验测定细胞毒性。1×10⁵个细胞和CAL-101一起温育。加入MTT试剂, 再次温育20小时，然后用溶于PBS的硫酸鱼精蛋白冲洗。加入DMSO, 用分光光度计在540纳米处测定吸光度。使用膜联蛋白/PI液式细胞计检查在不同时间点测定细胞存活力，分析数据。每个样本至少计数10⁴个细胞。以全部阳性细胞与未处理细胞之比百分数的形式来表示实验结果。加入100 μM Z-VAD检测caspase-依赖的细胞凋亡。加入 1 μg/mL CD40L, 800 U/mL IL-4, 50 ng/mL BAFF, 20 ng/mL TNF-α测定存活信号, 或者共培养在纤连蛋白或 HS-5 细胞系基质包被的板上。基质共培养在75cm²培养瓶 (80%-100% 融合率) 培养24 小时，然后加入CLL细胞。

参考文献	[1] Lannutti BJ, et al. Blood, 2011, 117(2), 591-594. [2] Herman SE, et al. Blood, 2010, 116(12), 2078-2088. [3] Meadows SA, et al. Blood, 2011 Dec 30.

溶解度（25°C）

体外


体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+20%PEG 300+ddH2O 点击购买： PEG300	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量	415.42
化学式	C₂₂H₁₈FN₇O
CAS号	870281-82-6
储存条件	3年	-20°C	粉状
储存条件	2年	-80°C	溶于溶剂

Download Idelalisib SDF

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

临床试验信息

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03582098	Completed	Drug: Idelalisib\|Drug: Rituximab	Chronic Lymphocytic Leukaemia	Gilead Sciences	September 12 2018	--
NCT03151057	Active not recruiting	Drug: Idelalisib 100 MG\|Drug: Placebo Oral Tablet	B Cells-Tumors\|B Cell Chronic Lymphocytic Leukemia\|Follicular Lymphoma\|Mantle Cell Lymphoma\|Large B-Cell Diffuse Lymphoma of Bone (Diagnosis)	Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins\|Gilead Sciences	July 31 2018	Phase 1
NCT03568929	Completed	Drug: Idelalisib	Follicular Non-Hodgkin''s Lymphoma Refractory	Gilead Sciences	May 25 2018	--

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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常见问题及建议解决方法

问题 1:
What is the recommended dose of CAL-101 and the route of administration for mouse studies?

回答：
According to the following paper, S2226 can be used by I.V. administration at the concentration of 40 mg/kg. https://www.ncbi.nlm.nih.gov/pubmed/24625684

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